• Title/Summary/Keyword: WAsP Engineering

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Design of an Advanced Kerbros P2P Authentication System to Share Digital Content (디지털 콘텐츠 공유를 위한 개선된 Kerberos P2P 인증시스템 설계)

  • Kim Jong-Woo;Han Seung-Jo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.7
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    • pp.1516-1523
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    • 2004
  • In the paper, an algorithm fitted to P2P system was proposed by improving Kerberos which is an algorithm for mutual authentication. To keep the role of Kerberos and minimize load to server, the proposed algorithm imposed the server role of ticket recognition to the opposite peer. Using this method, the number of sewers as ticket recognition server was averted and function of server for authentication was minimized so that server load was mininized. The proposed algorithm enables the server to play the minimum of the role and to perform strong mutual authentication, while imposeing on the peers the role of authentication. To make suitable to P2P system, trial number oriented authentication limit was given, not time-oriented authentication expiration time. In the paper, a new P2P system was designed using this algorithm.

JXTA Based P2P Services for Mobile Devices

  • Park, Hae-Woo;Jung, Hun;Seo, Yeung-Su;Yu, Chun-Gun;Lee, Jun-Hyuk;Kang, Byung-Wook
    • 한국정보컨버전스학회:학술대회논문집
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    • 2008.06a
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    • pp.195-198
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    • 2008
  • The JXTA was made in order to solve the problem of being generated with the computer platform suggested with the P2P(Peer-to-peer) method in the distributed computing. JXTA makes a communication between the various network and the devices is connected to a network which possible. It was designed to the client having the minimum computing ability could attend in the peer-to-peer network. However, for smoothly applying JXTA in the wireless environment, there is a limit with the limited resource of the Mobile Device. By using the Relay of the JXTA based for the Mobile Device, this paper implemented the P2P service which is flexibly effective in the wireless network environment.

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Zeta-potentials of Oxygen and Nitrogen Enriched Activated Carbons for Removal of Copper Ion

  • Park, Kwan-Ho;Lee, Chang-Ho;Ryu, Seung-Kon;Yang, Xiaoping
    • Carbon letters
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    • v.8 no.4
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    • pp.321-325
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    • 2007
  • The oxygen and nitrogen enriched activated carbons were obtained from modification of commercial activated carbon by using nitric acid, sodium hydroxide and urea. Zeta-potentials of modified activated carbons were investigated in relation to copper ion adsorption. The structural properties of modified activated carbons were not so much changed, but the zeta-potentials and isoelectric points were considerably changed. The zeta-potential of nitric acid modified activated carbon was the most negative than other activated carbons in the entire pH region, and the $pH_{IEP}$ was shifted from pH 4.8 to 2.6, resulted in the largest copper ion adsorption capacities compare with other activated carbons in the range of pH 3~6.5. In case of urea modified activated carbon, copper ion adsorption was larger than that of the as-received activated carbon from pH 2 to pH 6.5 even though the $pH_{IEP}$ was shifted to pH 6.0, it was due to the coordination process operated between nitrogen functional groups and copper ion. The adsorption capacity of copper ion was much influenced by zeta-potential and $pH_{IEP}$ of carbon adsorbent.

Enhancement of cis,cis-Muconate Productivity by Overexpression of Catechol 1,2-Dioxygenase in Pseudomonas putida BCM114

  • Kim, Beum-Jun;Park, Won-Jae;Lee, Eun-Yeol;Park, Cha-Yong
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.2
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    • pp.112-114
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    • 1998
  • For enhancement of cis,cis-muconate productivity from benzoate, catechol 1,2-dioxygenase (C12O) which catalyzes the rate-limiting step (catechol conversion to cis,cis-muconate) was cloned and expressed in recombinant Pseudomonas putida BCM114. At higher benzoate concentrations (more than 15 mM), cis,cis-muconate productivity gradually decreased and unconverted catechol was accumulated up to 10 mM in the cae of wild-type P. putida BM014, whereas cis,cis-muconate productivity continuously increased and catechol was completely transformed to cis,cis-muconate for P. putida BCM114. Specific C12O activity of P. putida BCM114 was about three times higher than that of P. putida BM014, and productivity was enhanced more than two times.

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Proposed dynamic p-y curves on a single pile considering shear wave velocity of soil

  • Song, Sumin;Lim, Hyunsung;Park, Seongyong;Jeong, Sangseom
    • Earthquakes and Structures
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    • v.23 no.4
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    • pp.353-361
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    • 2022
  • The dynamic behavior of a single pile was investigated by using analytical and numerical studies. The focus of this study was to develop the dynamic p-y curve of a pile for pseudo-static analysis considering the shear wave velocity of the soil by using three-dimensional numerical analyses. Numerical analyses were conducted for a single pile in dry sand under changing conditions such as the shear wave velocity of the soil and the acceleration amplitudes. The proposed dynamic p-y curve is a shape of hyperbolic function that was developed to take into account the influence of the shear wave velocity of soil. The applicability of pseudo-static analysis using the proposed dynamic p-y curve shows good agreement with the general trends observed by dynamic analysis. Therefore, the proposed dynamic p-y curve represents practical improvements for the seismic design of piles.

Indole Derivatives I, The Kinetics and Mechanism of the Nucleopilic Addition of Thiourea to 1-Benzylindole-3-Acetophenone (인돌유도체 I, 1-Benzylindole-3-acetophenone유도체에 대한 Thiourea의 친핵성 첨가반응 메카니즘과 그 반응속도론적 연구)

  • Lee, Ki-Chang;Hwang, Sung-Kwu;Ryu, Jung-Wook;Hwang, Young-Hyun;Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.8 no.2
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    • pp.175-181
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    • 1991
  • The kinetics of the addition of 1-benzylindole-3-(p-substituted) acetophenone derivatives was investigated by ultraviolet spectrophotometery in 30% dioxane -$H_2O\;at\;25^{\circ}C$. A rate equation which can be applied over wide pH range was obtained. The Substituent effects on 1-benzylindole-3-(p-substituted) acetophenone derivatives were studied, and addition were facilitated by electron attracting groups. On the base of the rate equation, substituent effect, and general base effect the plausible addition mechanism was proposed : Below pH 3.0, only neutral thiourea molecule was added to the carbon-carbon double bond, and in the range of pH 0.0${\sim}$14.0, netural thiourea molecule and thiourea anion competitively attacted the double bond. By contrast, above pH 10.0, the reaction was dependent upon only the addition of thiourea anion.

Nucleotide Sequence Analyses of p10 Gene and its Promoter of Hyphantria cunea Nuclear Polyhedrosis Virus (Hyphantria cunea Nuclear Polyhedrosis Virus p10유전자와 프로모터의 염기서열 결정)

  • Park, Sun-A;Cha, Sung-Chul;Chang, Jae-Hyeok;Lee, Hyung-Hoan
    • The Journal of Korean Society of Virology
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    • v.26 no.1
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    • pp.131-137
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    • 1996
  • The sequences of p10 gene its promoter of Hyphantria cunea NPV were determined. According to the sequence analysis, the putative p10 gene ORF has 285 bp. The 5'-non-coding leader sequence of the p10 gene promoter contained the TATA box and the putative transcription initiation site TAAG motif. Poly (A) tail signals, AATAAA sequence was at site 65 base upstream from the 3' terminus. The deduced amino acid sequence of p10 protein was 95 with a predicted molecular weight of 10.26 kDa. In the p10 protein sequence, a hydrophobic region was present at the N-terminus of the protein, whereas the C-terminus was highly hydrophilic. The p10 protein of H. cunea NPV did not contain cysteine, histidine, trytophan, tryptophane, tyrosine, glutamine and asparagine residues.

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Roles of Putative Sodium-Hydrogen Antiporter (SHA) Genes in S. coelicolor A3(2) Culture with pH Variation

  • Kim, Yoon-Jung;Moon, Myung-Hee;Lee, Jae-Sun;Hong, Soon-Kwang;Chang, Yong-Keun
    • Journal of Microbiology and Biotechnology
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    • v.21 no.9
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    • pp.979-987
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    • 2011
  • Culture pH change has some important roles in signal transduction and secondary metabolism. We have already reported that acidic pH shock enhanced actinorhodin production in Streptomyces coelicolor. Among many potential governing factors on pH variation, the putative $Na^+/H^+$ antiporter (sha) genes in S. coelicolor have been investigated in this study to elucidate the association of the sha on pH variation and secondary metabolism. Through the transcriptional analysis and overexpression experiments on 8 sha genes, we observed that most of the sha expressions were promoted by pH shock, and in the opposite way the pH changes and actinorhodin production were enhanced by the overexpression of each sha. We also confirmed that sha8 especially has a main role in maintaining cell viability and pH homeostasis through $Na^+$ extrusion, in salt effect experiment under the alkaline medium condition by deleting sha8. Moreover, this gene was observed to have a function of pH recovery after pH variation such as the pH shock, being able to cause the sporulation. However, actinorhodin production was not induced by the only pH recovery. The sha8 gene could confer on the host cell the ability to recover pH to the neutral level after pH variation like a pH drop. Sporulation was closely associated with this pH recovery caused by the action of sha8, whereas actinorhodin production was not due to such pH variation patterns alone.

Construction of Shuttle Promoter-probe and Expression Vectors for Escherichia coli and Bacillus subtilis, and Expression of B. thuringiensis subsp. kurstaki HD-73 Crystal Protein Gene in the Two Species

  • Park, Seung-Hwan;Koo, Bon-Tag;Shin, Byung-Sik;Kim, Jeong-Il
    • Journal of Microbiology and Biotechnology
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    • v.1 no.1
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    • pp.37-44
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    • 1991
  • A shuttle promoter-probe vector, pEB203, was derived from pBR322, pPL703 and pUB110. Using the vector, a useful DNA fragment, 319 bp EcoRI fragment, having strong promoter activity has been cloned from Bacillus subtills chromosomal DNA. Selection was based on chloramphenicol resistance which is dependent upon the introduction of DNA fragments allowing expression of a chloramphenicol acetyl transferase gene. The nucleotide sequence of the 319 bp fragment has been determined and the putative -35 and -10 region, ribosome binding site, and ATG initiation codon were observed. This promoter was named EB promoter and the resultant plasmid which can be used as an expression vector was named pEBP313. The crystal protein gene from B. thuringiensis subsp. kurstaki HD-73 was cloned downstream from the EB promoter without its own promoter. When the resultant plasmid, pBT313, was introduced into Escherichia coli and B. subtilis, efficient synthesis of crystal protein was observed in both cells, and the cp gene expression in B. subtilis begins early in the vegetative phase. The cell extracts from both clones were toxic to Hyphantria cunea larvae.

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The Characteristics of SBR Treatment with Different Types of Piggery Wastewater (축산폐수성상에 따른 SBR 처리특성)

  • Jun, Byong-Hee;Poo, Kyung-Min;Kim, Yang-Hoon;Im, Jeong-Hoon;Kim, Chang-Won
    • Journal of Korean Society on Water Environment
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    • v.18 no.4
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    • pp.435-440
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    • 2002
  • A pilot scale SBR (effective volume, $20m^3$) for the treatment of piggery wastewater treatment was performed with three different kinds of wastewater; fermenter effluent, scraper type and slurry type. The react phase in SBR was performed by sub-cycle operation consisting of repeated short cycle of anoxic-aerobic step. The fermenter effluent was characterized by the rapid nitrification and $NO_X-N$ accumulation due to depletion of organic matter in wastewater. The scraper type wastewater showed appropriate nitrogen removal efficiency, however, a poor response capacity for high loading rate often resulted in increased nitrogen concentration in effluent. Moreover, severe P release was the most serious problem in scraper type wastewater. SBR treated slurry type wastewater with high nitrogen removal efficiency to satisfy effluent quality requirement. It was thought that high concentration of organic matter in slurry made it possible to uptake P during SBR operation, where P concentration of 140mgP/l was decreased to 8mgP/l. As results, SBR was suitable to treat slurry type wastewater which has been discharged to the ocean till now.