Purpose : Investigate the effects of Haeganjeon water extract (HGJ) on immobilization-stress or cold-stress in C57BL/6J mice. Methods : Male C57BL/6J 30 mice of weighting 18${\pm}$2g, were divided into sixs groups including the immobilization-stress group(5heads), after immobilization-stress HGJ oral administration(500mg/kg) groups(5heads), cold-stress group(5heads) and after cold-stress HGJ oral administration(500mg/kg) groups(5heads). then we observed changes in the serum histamine and corticosterone level and changes immune system. Results : HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells. In addition, HGJ enhanced the cell viability of thymocytes decreased by immobilization-stress or cold-stress and decreased DNA fragmentation of thymocytes increased by immobilization- stress or cold-stress. Also, HGJ increased the cell viability of splenocytes decreased by cold-stress and decreased DNA fragmentation of splenocytes increased by cold-stress. HGJ decreased the population of thymic CD4+ cells increased by immobolization-stress. HGJ increased the population of B220+ cells decreased by immobilization-stress and decreased the population of Thy1+ cells increased by immobilization-stress. Also, HGJ decreased the population of splenic CD4+ cells increased by immobolization-stress. HGJ enhanced the production of ${\gamma}-interferon$ decreased by immobilization-stress or cold-stress and increased the production of interleukin-4 decreased by immobilization-stress. Furthermore, HGJ enhanced the phagocytic activity decreased by immobilization-stress or cold-stress and enhanced the level of nitric oxide decreased by immobilization-stress or cold-stress. Conclusion : HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.
This atudy was conducted to determine the growth characteristics and photosynthesis of soybean (Glycine max L. cv. Keumjongkongl) 30 day old seedling to 100mM NaCl concentration containing 1/2 Hoagland`s nutrient solution in sand culture. The nodule formation of root is not found perfectly with NaCl stress. The leaf dry matter weight (g/plant) of stressed plant is more reduction in 77% to control than any other characters. The water content (%) is tend to increase but water potential (MPa) is tend to decrease at NaCl stress. The chlorophyll content (SPAD) is tend to increase at growing leaf age of control but decrease at NaCl stress. The photosynthesis, stomatal conductance and transpiration are tend to decrease sharply at NaCl stress.
This experiment was carried out on plastic pots ($40cm{\times}25cm{\times}30cm$) filled with sand soil at greenhouse using two soybean cultivars with small seed; one was Pungsannamulkong (PSNK) recognized as a tolerant cultivar against excessive water stress and the other one was Sobaeknamulkong (SBNK) recognized as a susceptible cultivar. Seed was sown with 30 plants of 2 hills, and the amount of applied fertilizer was N; 3.0 g, P; 3.0 g, and K; 3.4 g per $m^2$ with all basal fertilizations. Plants were grown under photoperiod of natural light with day temperature of $31{\pm}5^{\circ}C$ and night temperature of $22{\pm}1^{\circ}C$. The flooding treatment was done for 3, 5, 7 and 10 days by filling pots with tap water up to 1 cm above the level of the soil surface when plants were 2 days after emerging. Nitrogen uptake by leaves of soybeans decreased significantly by the flooding after 6 days. This significant reduction of N uptake by flooding was evidently recognized from the chlorosis of leaves. The dry matter of flooded soybean seedlings significantly decreased compared to non-flooded soybean seedlings at 10 days. The dry matter of roots also showed similar result of the shoot. Shoots had more N reduction than roots under the flooding. This N reduction was more pronounce in SBNK than in PSNK. Chlorophyll content of flooded soybeans showed decreasing or non-increasing tendency, and the reduction of chlorophyll content was more in SBNK than in PSNK from the flooding stress. Nitrate content of soybean seedlings with flooding stress showed decreasing tendency in shoot and root parts. Ammonium content, however, was higher in flooding stress compared to the non-flooding. Flooding caused a remarkable change in the AA (amino acid) composition and TAA (total amino acid) concentration in the leaves of soybean seedlings.
We examined changes in the physiological responses of gray mullet Mugil cephalus exposed to acidic seawater (pH 6.0, 6.5, 7.0) and normal seawater (pH 8.0, control) for 15 days. As pH decreased, survival rate and body weight also decreased. Levels of aminotransferase, total protein and triglycerides also differed significantly with changes in pH, presumably due to stress caused by exposure to acidic water. The level of osmotic pressure was significantly higher in the pH 6.0 group than in other groups. Superoxide dismutase was significantly higher in the pH 6.5 and 7.0 groups than in the pH 8.0 group, and glutathione level was lowest in the pH 6.0 group. We conclude that decreasing the pH level of seawater induces a stress response in fish, damaging their ability to control their hematological and osmotic pressure. Antioxidant enzymes are generally sensitive to osmotic stress; in this study, antioxidant activity significantly changed with pH level. These results indicate that physiological stress induced by exposure to acidification reduces survival rates and inhibits growth in M. cephalus.
The stress dependence of ultrasonic wave velocity is known as the acoustoelastic effect. This effect is useful for stress monitoring if the acoustoelastic coefficient of a subject medium is known. The acoustoelastic coefficients of metallic materials such as steel have been studied widely. However, the acoustoelastic coefficient of concrete has not been well understood yet. Basic constituents of concrete are water, cement, and aggregates. The mix proportion of those constituents greatly affects many mechanical and physical properties of concrete and so does the acoustoelastic coefficient of concrete. In this study, influence of the water-cement ratio (w/c ratio) and the fine-coarse aggregates ratio (fa/ta ratio) on the acoustoelastic coefficient of concrete was investigated. The w/c and the fa/ta ratios are important parameters in mix design and affect wave behaviors in concrete. Load-controlled uni-axial compression tests were performed on concrete specimens. Ultrasonic wave measurements were also performed during the compression tests. The stretching coda wave interferometry method was used to obtain the relative velocity change of ultrasonic waves with respect to the stress level of the specimens. From the experimental results, it was found that the w/c ratio greatly affects the acoustoelastic coefficient while the fa/ta ratio does not. The acoustoelastic coefficient increased from $0.003073MPa^{-1}$ to $0.005553MPa^{-1}$ when the w/c ratio was increased from 0.4 to 0.5. On the other hand, the acoustoelastic coefficient changed in small from $0.003606MPa^{-1}$ to $0.003801MPa^{-1}$ when the fa/ta ratio was increased from 0.3 to 0.5. Finally, it was also found that the relative velocity change has a linear relationship with the stress level of concrete.
Journal of the Korea Institute of Building Construction
/
v.18
no.3
/
pp.235-242
/
2018
The objective of the paper is to investigate the effect of mix designing factors on the workability and rheological parameters of self compacting concrete in order to facilitate the difficulties of quality control of high sensitivity of SCC. Mix proportions of SCC were prepared with various conditions of coarse, and fine aggregate, and unit water content, and the SCC mixtures were tested on workability, rheological properties to provide basic data for quantitative evaluation. Test results indicated that the yield stress of SCC decreased with increasing the coarse aggregate volume ratio, and increased with increasing the amount of VMA. However, unit water content, fine aggregate type, and air content didn't affect the yield stress value. The plastic viscosity according to the mixing factors showed a similar tendency to the yield stress. In addition, there was no correlation between yield stress and workability (flow, T50, V-lot). However, there was closely correlation among plastic viscosity and T50 and V-lot. Especially, T50 and V-lot time decreased with decreasing plastic viscosity.
Jin, Chang Hyun;Cho, Byoung Ok;Choi, Dae Seong;Ryu, Hyung Won;Baek, Ji Yeong;Jeong, Il Yun
Journal of Radiation Industry
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v.5
no.2
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pp.125-130
/
2011
The purpose of this study was to evaluate the protective effect of the Perilla frutescens cv. Chookyoupjaso mutant water extract (PFWE) on gamma ray-induced oxidative stress in mice. Gamma-ray is one of the sources for inducing oxidative stress. The study was divided into 6 groups with 6 mice for each treatment. Groups I and II were treated with saline (vehicle) only, groups III, IV, V, and VI were pretreated with PFWE 10, 20, 50, $50mg\;kg^{-1}$ respectively for 2 weeks before gamma radiation. And then groups II, III, IV, V were irradiated. We found that the activities of aspartate transaminase (AST) and alanine transaminase (ALT) were increased and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were decreased by irradiation in mice. However, treatment of PFWE attenuated the activities of AST and ALT in a dose-dependent manner in irradiated mice. Furthermore, treatment of PFWE significantly increased the activities of SOD, GPx, and GR in a dose-dependent manner in irradiated mice, except for the CAT. Interestingly, the activities of GPx and GR were significantly increased by PFWE treatment. Taken together, PFWE could be effective in protecting on gamma ray-induced oxidative stress in mice.
Climate change, driven by the natural process of global warming, is a worldwide issue of significant concern because of its adverse effects on livestock output. The increasing trend of environmental temperature surging has drastically affected meat production and meat product quality, hence result in economic losses for the worldwide livestock business. Due to the increasing greenhouse gas emissions, the situation would get prolonged, and heat exposure-related stress is expected to worsen. Heat exposure causes metabolic and physiological disruptions in livestock. Ruminants and monogastric animals are very sensitive to heat stress due to their rate of metabolism, development, and higher production levels. Before slaughter, intense hot weather triggers muscle glycogen breakdown, producing pale, mushy, and exudative meat with less water-holding capacity. Animals exposed to prolonged high temperatures experience a decrease in their muscle glycogen reserves, producing dry, dark, and complex meat with elevated final pH and increased water-holding capacity. Furthermore, heat stress also causes oxidative stresses, especially secondary metabolites from lipid oxidation, severely affects the functionality of proteins, oxidation of proteins, decreasing shelf life, and food safety by promoting exfoliation and bacterial growth. Addressing the heat-related issues to retain the sustainability of the meat sector is an essential task that deserves an inclusive and comprehensive approach. Considering the intensity of the heat stress effects, this review has been designed primarily to examine the consequences of hot environment temperatures and related stresses on the quality and safety of meat and secondarily focus on cutting edge technology to reduce or alleviate the situational impact.
Proceedings of the Korean Geotechical Society Conference
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2003.03a
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pp.257-264
/
2003
Tunnelling in water bearing soils influences the ground water regime. It has been indicated in the literature that the existence of ground water above a tunnel influences tunnel stability and the settlement profile. Only limited research, however, has been done on ground water movements around tunnels and their influence on tunnel performance. Time dependent soil behaviour can be caused by the changes of pore water pressure and/or the viscous properties of soil(creep) under the stress change resulting from the advance of the tunnel face. De Moor(1989) demonstrated that the time dependent deformations due to tunnelling are mainly the results of pore pressure dissipation and should be interpreted in terms of effective stress changes. Drainage into tunnels is governed by the permeability of the soil, the length of the drainage path and the hydraulic boundary conditions. The potential effect of lime dependent settlement in a shallow tunnel is likely to occur rapidly due to the short drainage path and possibly high coefficient of consolidation. Existing 2D modelling methods are not applicable to these tunnelling problems, as it is difficult to define empirical parameters. In this paper the time-based 2D modelling method is adopted to account for the three dimensional effect and time dependent behaviour during tunnel construction. The effect of coupling between the unloading procedure and consolidation during excavation is profoundly investigated with the method. It is pointed out that realistic modelling can be achieved by defining a proper permeability at the excavation boundary and prescribing appropriate time for excavation Some guidelines for the numerical modelling of drained and undrained excavation has been suggested using characteristic time factor. It is highlighted that certain range of the factor shows combined effect between the unloading procedure due to excavation and consolidation during construction.
With the increasing tension of current coal resources and the increasing depth of coal mining, the gob-side entry retaining technology has become a preferred coal mining method in underground coal mines. Among them, the technology of the gob-side entry retaining with the high-water filling material can not only improve the recovery rate of coal resources, but also reduce the amount of roadway excavation. In this paper, based on the characteristics of the high-water filling material, the technological process of gob-side entry retaining with the high-water filling material is introduced. The early and late stress states of the filling body formed by the high-water filling materials are analyzed and studied. Taking the 8th floor No.3 working face of Xin'an coal mine as engineering background, the stress and displacement of surrounding rock of roadway with different filling body width are analyzed through the FLAC3D numerical simulation software. As the filling body width increases, the supporting ability of the filling body increases and the deformation of the surrounding rock decreases. According to the theoretical calculation and numerical simulation of the filling body width, the filling body width is finally determined to be 3.5m. Through the field observation, the deformation of the surrounding rock of the roadway is within the reasonable range. It is concluded that the gob-side entry retaining with the high-water filling material can control the deformation of the surrounding rock, which provides a reference for gob-side entry retaining technology with similar geological conditions.
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