• Title/Summary/Keyword: WATER STRESS

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Protective Effects of Omijatang on Oxidative Stress-Induced Apoptosis of H9c2 Cardiomyoblast Cells (오미자탕(五味子湯)이 산화적 손상으로 유발된 세포고사에 미치는 영향)

  • Choi, Jin-Young;Shin, Sun-Ho;Lee, Yun-Jae
    • The Journal of Internal Korean Medicine
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    • v.26 no.2
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    • pp.420-430
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    • 2005
  • The water extract of Omijatang(OMJT) has been traditionally used for treatment of abscess and heart palpitation in oriental medicine, However, little is known about the mechanism by which the water extract of OMJT rescues cells from these damages. This study was designed to investigate the protective mechanisms of OMJT in H9c2 cardiomyoblasts on oxidative stress-induced cytotoxicity including $H_2O_2,\;ZnCl_2$, hypoxia, and reoxygenation. Oxidative stress markedly decreased the viability of H9c2 cells. This was characterized with apparent apoptotic features such as chromatin condensation as well as fragmentation of genomic DNA and nuclei. However, OMJT significantly reduced $H_2O_2$-induced cell death and apoptotic characteristics as well as $ZnCl_2$, hypoxialreoxygenation. Taken together, this study suggests that the water extract of OMJT has the protective effects against oxidative injuries.

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Numerical Simulation of Temperature and Stress Distribution in Mass Concrete with pipe cooling and Comparision with Experimental Measurements (매스콘크리트 시험체의 수화열 해석 및 실험)

  • 주영춘;김은겸;신치범;조규영;박용남
    • Proceedings of the Korea Concrete Institute Conference
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    • 1999.04a
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    • pp.269-274
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    • 1999
  • Various method have been developed for mass concrete structures to reduce the temperature increase of concrete mass due to exothermic hydration reactions of concrete compounds and thereby to avoid thermal cracks. One of the methods widely acceptable for practical use is pipe cooling, in which cooling is achieved by circulating cold water through thin-wall steel pipes embedded in the concrete. A numerical simulation was performed to investigate the effectiveness of pipe cooling. A three-dimensional finite element method was proposed to analyse the transient three-dimensional heat transfer between the hardening concrete and the cooling water in pipe and to predict the stress development during the curing process. The effects of the cement type and content and the environment were taken into consideration by the heat generation rate and the boundary conditions, respectively. In order to test the validity of the numerical simulation, a model RC structure with pipe cooling was constructed and the time-dependent temperature and stress distributions within the structure as well as the variation of the temperature of cooling water along the pipe were measured. The results of the simulation agreed well the experimental measurements. The results of this study have important implications for the optimal design of the cooling pipe layout and for the estimation of thermal stress in order to eliminate thermal cracks.

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Effect of Dissolved Oxygen on the Stress Cor rosion Cracking Behavior of 3.5NiCrMoV Steels in High Temperature Water

  • Lee, J.H.;Maeng, W.Y.;Kim, U.C.
    • Corrosion Science and Technology
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    • v.2 no.4
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    • pp.178-182
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    • 2003
  • Slow Strain Rate Tests (SSRT) were carried out to investigate the effect of environmental factors on the Stress Corrosion Cracking (SCC) susceptibility of 3.5NiCrMoV steels used in discs for Low-Pressure (LP) steam turbines in electric power generating plants. The influences of dissolved oxygen on the stress corrosion cracking of turbine steel were studied, For this purpose, specimens were strained at variously oxygenated conditions at $150^{\circ}C$ in pure water. When the specimen was strained with $1{\times}10^{-7}s^{-1}$ at $150^{\circ}C$ in pure water, increasing concentration of dissolved oxygen decreased the elongation and the UTS. The corrosion potential and the corrosion rare increased as the amounts of dissolved oxygen increased. The increase of the SCC susceptibility of the turbine steel in a highly dissolved oxygen environment is due to the non protectiveness of the oxide layer on the turbine steel surface and the increase of the corrosion current. These results clearly indicate that oxygen concentration increases Stress Corrosion Cracking susceptibility in turbine steel at $150^{\circ}C$.

Characterization of Chitinase in Oak Tissues and Changes in Its Activity Related to Water Stress and Inoculation with Hypoxylon atropunctatum

  • Chun, Se-Chul;Fenn, Patrick;Kim, Kyung-Soo
    • The Plant Pathology Journal
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    • v.15 no.3
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    • pp.144-151
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    • 1999
  • Chitinase activities from Shumard oak tissues were determined to study changes in chitinase activities related to water stress. The enzyme extracted in sodium acetate buffer (0.1M, pH 4.5) was assayed by a colorimetric method. In addition, the fungal hyphae of Hypoxylon atropunctatum in xylem tissues of oak were observed through scanning electron microscopy. The enzyme in oak tissues was mainly endochitinase, and optimum pH for enzyme activity was 5. Specific chitinase activities from both of stems held under high relative humidity (ranges of 0.63-1.11 pKatal/$\mu\textrm{g}$ of protein) and stems held under low relative humidity (ranges of 0.41-0.99 pKatal/$\mu\textrm{g}$ of protein) were significantly increased following fungal inoculation with H. atropunctatum. However, there was no significant difference in chitinase activities between tissues held under high and low humidities, which might be due to fungal chitinase. Scanning electron microscopy showed holes in fungal hyphae in the xylem tissues of stems held under high humidity but not in the stems held under ow humidity, suggesting that hyphae might be hydrolyzed by plant hydolases such as chitinase.

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Effects of Erythropoietin on Memory Deficits and Brain Oxidative Stress in the Mouse Models of Dementia

  • Kumar, Rohit;Jaggi, Amteshwar Singh;Singh, Nirmal
    • The Korean Journal of Physiology and Pharmacology
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    • v.14 no.5
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    • pp.345-352
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    • 2010
  • The present study was undertaken to explore the potential of erythropoietin in memory deficits of mice. Memory impairment was produced by scopolamine (0.5 mg/kg, $i.p.$) and intracerebroventricular streptozotocin (i.c.v STZ, 3 mg/kg, $10{\mu}l$, $1^{st}$ and $3^{rd}$ day) in separate groups of animals. Morris water-maze test was employed to assess learning and memory. The levels of brain thio-barbituric acid reactive species (TBARS) and reduced glutathione (GSH) were estimated to assess degree of oxidative stress. Brain acetylcholinesterase enzyme (AChE) activity was also measured. Scopolamine/streptozotocin administration induced significant impairment of learning and memory in mice as indicated by marked decrease in Morris water-maze performance. Scopolamine/streptozotocin administration also produced a significant enhancement of brain AChE activity and brain oxidative stress (an increase in TBARS and a decrease in GSH) levels. Treatment of erythropoietin (500 and 1,000 IU/Kg i.p.) significantly reversed scopolamine- as well as streptozotocin-induced learning and memory deficits along with attenuation of those-induced rise in brain AChE activity and brain oxidative stress levels. It may be concluded that erythropoietin exerts a beneficial effect in memory deficits of mice possibly through its multiple actions including potential anti-oxidative effect.

Filtering System Design and Structural Analysis for Intake Water of Ship's Ballast Tank (선박 밸러스트 탱크 유입수 필터링 시스템 설계 및 구조해석)

  • Yun, Sang-Kook;Park, Byung-Geun
    • Journal of Advanced Marine Engineering and Technology
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    • v.33 no.2
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    • pp.282-287
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    • 2009
  • As current international guideline and IMO regulation give severe restrictions for ships to manage ballast water to reduce unintentional organism transfers, several ballast water treatment systems recently have been being developed together with filtration. That is because discharging ballast water from ships causes many pollutions by foreign biological invasive species. The primary treatment system being considered in this study was based on fine screen filtration technology applied to ballast water filter in ballast tank in order to reduce the load of ballast water treatment system. New ballast water filtration system was invented and analysed. The structural stress and strain analysis for ballast filtration systems which are current and invented filters were carried out using UGS and Ansys. The results showed that the structure of current filtering module was not designed to meet the requirement of sea water filtration during ballasting operation. The studies also showed that the invented design of filtration system equipped with back washing and automatic scrapper for eliminating cake of bio-species might be a potentially effective technology for ballast water management of ship's ballast tank.

Relationship Between Relative Water Content and Ascorbate Redox Enzymes Activity in Lettuce Leaves Subjected to Soil Water Stress (토양 수분 Stress에 따른 상추의 엽중 상대수분 함량과 아스코브산 관련 효소 활성도)

  • Kang, Sang-Jae;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.32-39
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    • 2013
  • The relationship between relative water contents of lettuce leaves and biochemical activities in lettuce was examined in this study to explore an adaptation response of lettuce to water stress from soils. Soil water contents and relative water contents of leaves were positively related to show $R^2$=0.8728. Hydrogen peroxide contents of leaves rapidly increased with reduction of soil water content, whereas soluble protein contents and dry matters rapidly decreased. And chlorophyll a and b contents of leaves decreased with increase in carotenoid content. Furthermore, the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) increased dramatically, and mRNA transcript levels of APX, MDHAR and DHAR also increased. Relationship of relative water content of lettuce leaves to hydrogen peroxide, to ascorbate peroxidase activity, to dehydroascorbate reductase activity, and to monodehydroascorbate reductase activity was shown to be positively correlated. It is highly plausible from this study that these enzyme activities could be developed as an indicator of water states in soils.

Effects of the Vibration Stress on Cortisol and Hematological Characteristics in Soft-shelled Turtle, Pelodiscus sinensis (진동 스트레스에 따른 자라, Pelodiscus sinensis의 코티졸 및 혈액학적 특성)

  • Hur, Jun-Wook;Lee, Jeong-Yeol;Chang, Young-Jin;Bai, Sung-Chul;Park, In-Seok
    • Development and Reproduction
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    • v.13 no.1
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    • pp.43-49
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    • 2009
  • We examined the effects of the vibration stress on cortisol secretion and hematological characteristics in soft-shelled turtle, Pelodiscus sinensis. For the stressed group vibration of $45{\sim}78 dB(V)$ from electric vibrator applied for 30 min with 2-h intervals during daytime ($08:00{\sim}18:00$) up to 28 days. Using the blood samples collected from ten turtles held once a week after vibration stress, we measured hematocrit, hemoglobin, red blood cells, cortisol, glucose, lactic acid, osmolality, $Na^+,\;K^+,\;Cl^-$, aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results have showed that P. sinensis received vibration stress exhibit the 'typical' stress-induced physiological responses (cortisol, glucose, lactic acid, osmolality, ions, hematocrit and hemoglobin) induced by vibration stress. Our data suggested that chronic vibration stress caused substantial stress in the animal, and in particular, the persisting elevated levels of AST and ALT would be highly correlated with the adverse effects of the stress. The high hematological characteristics during entire experimental period showed that the P. sinensis could not adapt to chronic stimuli provoked by vibration stress.

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Elastic Modeling for the Behavior of Undrained Pore Water Pressure in Saturated Sand (포화된 사질토에서 비배수 공극수압거동에 대한 탄성해석모델의 개발)

  • Eam, Sung-Hoon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.47 no.5
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    • pp.43-49
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    • 2005
  • In this study. it was suggested that the elastic model to analyze the behavior of pore water pressure in saturated sand specimen on the condition of non-drainage. The model based on the experiments which were performed for the relationships between the pore water pressure and the grain size of specimen, and effective stress, respectively. The suggested model embodied the pore water and soil grain as separate elastic springs of different stiffness. The springs were joined parallel and the axial strains were restricted to the same deformation. The suggested model was well consistent with the experiments.

Rheological Properties According to the Hardness of Dampening solution for Lithographic Ink (축임물의 경도에 따른 평판인쇄잉크의 유동성 변화)

  • Cho, Jin-Woo;Kim, Sung-Bin;Park, Jeung-Min
    • Journal of the Korean Graphic Arts Communication Society
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    • v.20 no.2
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    • pp.7-18
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    • 2002
  • The emulsification of lithographic printing inks are very important to determine printing quality. In this study, we measured the variation of water pickup according to the hardness of water in dampening solution. In order to observe the rheorogical properties of the emulsified inks, we measured the viscosity, the elastic return, the thixotropy area and yield stress value of the emulsified inks. In this paper, we found that the water pickup of the ink increase the hardness of water with the amount of dampening solution. Also, The viscosity, the yield stress value and the thixotropy area were increase by the water pickup of the ink.

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