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Effect of an Acid pH Shock on Physiological Changes of Chlamydomonas acidophila (Chlorophyta), UTCC 122 (pH 충격에 의한 Chlamydomonas acidophila (Chlorophyta), UTCC 122의 생리적 변화에 관한 연구)

  • Lee, Kyung;Ki, Jang-Seu;Kim, Say-Wa;Han, Myung-Soo;Choi, Young-Kil;Yoo, Kwang-Il
    • Korean Journal of Ecology and Environment
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    • v.35 no.3 s.99
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    • pp.145-151
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    • 2002
  • The effect of low pH on physiological changes was studied with the acidophilic green alga, Clamydomonas acidophila, UTCC 122. The growthrates (${\mu}$) were identical, $0.5{\sim}0.7\;day^{-1}$, at pH 3.7${\sim}$6.7 and no significantly different (ANOVA, p =0.134), showing cell volume reduced gradually as they were growing, whereas that at pH 2.7 was falling to zero and cell volume increased dramatically. Chlorophyll a concentration of the cultures incubated for one day was $191{\sim}255\;pg\;cell^{-1}$, after then it declined from $60{\sim}103\;pg\;cell^{-1}$ at pH 3.7${\sim}$6.7 except $210\;pg\;cell^{-1}$ at pH 2.7, which was directly related with cell volume. External carbonic anhydrase (CA) activity was varied from1.1 to$3.7{\times}10^{-4}\;E.U.\;mm^{-2}$, showing the gradualincrease during culture, except at 2.7 and pH 5.7. However there was not found any relationship among the pH gradient cultures. CA molecular mass of C. acidophila was 29 kBa, and concentration of that was identical in all cultures. The proteins of 41 kDa and 63 were not or very faintly expressed in low pH cultures, in contrast that of 17 kDa more expressed. In this work, we found that C. acidophila could live optimally within a wide range of acidic pH, and 17 kDa of unidentified protein might be concerned with tolerating in low acid environment.

Preparation of Drinkable Yoghurt Added with Green Tea Powder (가루녹차를 첨가한 Drinkable Yoghurt의 제조)

  • Jung Da-Wa;Park Shin-In
    • The Korean Journal of Food And Nutrition
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    • v.18 no.4
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    • pp.349-356
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    • 2005
  • For the purpose of making a new type of functional drinkable yoghurt, skim milk containing $0.5\~2.0\%$(w/v) green tea powder was fermented by the mixed stains of Streptococcus thermophilus and Lactobacillus acidophilus. Quality characteristics of the drinkable yoghurts were evaluated in terms of quality-keeping properties(number of viable cells, pH, titratable acidity) and sensory properties. When the drinkable yoghurts added with $0.5\~2.0\%$ green tea powder were kept at $4^{\circ}C\;and\;20^{\circ}C$ for 20 days, the number of viable cell counts of the lactic acid bacteria($2.1\times10^8\~6.2\times10^8$ CFU/mL). pH($4.16\~4.22$) and titratable acidity($0.792\~0.881\%$) were not significantly changed for all drinkable yoghurts during the storage at $4^{\circ}C\;and\;20^{\circ}C$ days, but the number of viable cell counts($4.2\times10^3\~1.8\times10^5$ CFU/mL), pH($3.82\~3.92$) and titratable acidity($1.057\~1.174\%$) were markedly changed f3r the storage at $20^{\circ}C$ for 20 days. Therefore the keeping quality of the drinkable yoghurts with addition of green tea powder was relatively good at $4^{\circ}C$ for 20 days. The results of sensory evaluation of the drinkable yoghurts containing peen tea powder indicated that flavor, sweet taste, mouthfeel and aftertaste of the drinkable yoghurt with $0.5\%$ green tea powder showed higher preference than others. And the drinkable yoghurt containing $0.5\% green tea powder added $20\%$(v/v) oligosaccharide had the higher sensory scores in sweet taste, aftertaste and overall acceptability among the treatments.

Evaluation of Carbon Sequestration Capacity of a 57-year-old Korean Pine Plantation in Mt. Taeh wa based on Carbon Flux Measurement Using Eddy-covariance and Automated Soil Chamber System (에디 공분산 및 자동화 토양챔버 시스템을 이용한 탄소 플럭스 관측 기반 태화산 57년생 잣나무조림지의 탄소흡수능력 평가)

  • Lee, Hojin;Ju, Hyungjun;Jeon, Jihyeon;Lee, Minsu;Suh, Sang-Uk;Kim, Hyun Seok
    • Journal of Korean Society of Forest Science
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    • v.110 no.4
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    • pp.554-568
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    • 2021
  • Forests are the largest carbon (C) sinks in terrestrial ecosystems. Recently, as enhancing forest C sequestration capacity has been proposed as a basic direction of the Republic of Korea's "2050 Carbon Neutral Strategy," accurate estimation of forest C sequestration has been emphasized. According to the Intergovernmental Panel on Climate Change guidelines, sequestration quantity is calculated from changes in C stocks in forest C pools, such as biomass, deadwood, litter and soil layer, and harvested wood products. However, in Korea, only the overstory biomass increase is now considered the amount of sequestration quantity, so there can be a significant difference from the actual forest C sequestration. In this study, we quantified forest C exchange through C flux measurement using an eddy covariance system and an automated soil chamber system in a 57-year-old Korean pine plantation located in Mt. Taehwa, Gwangju-si, Gyeonggi-do. Then, the net amount of C sequestration was compared with the amount of the overstory biomass increase. We estimated the annual C stock change in the remaining C pools by comparing the net sequestration amount from the C flux measurement with the overstory biomass increase and C stock change in the litter layer. Therefore, the net C sequestration of the Korean pine plantation estimated from the flux measurement was 5.96 MgC ha-1, which was about 2.2 times greater than 2.77 MgC ha-1 of the overstory biomass increase. The annual C stock increase in the litter layer was estimated to be 0.75 MgC ha-1, resulting in a total annual C stock increase of 2.45 MgC ha-1 in the remaining C pools. Our results indicate that the domestic forest is a larger C sink than the current methods, implying that more accurate calculations of the C sequestration capacity are necessary to quantify C stock changes in C pools along with the C flux measurement.

Productivity and Cost of Mechanized Felling and Processing Operations Performed with an Excavator-based Stroke Harvester by Tree Species (수종에 따른 스트로크 하베스터의 벌도⋅조재작업 생산성 및 비용)

  • Yun-Sung, Choi;Min-Jae, Cho;Ho-Seong, Mun;Jae-Heun, Oh
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.567-582
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    • 2022
  • Chainsaw use for motor-manual timber harvesting in South Korea is associated with worker safety issues. However, forestry operations such as timber harvesting have already been mechanized to reduce hazards to workers and increase productivity. This study analyzed the productivities and costs of felling and processing, felling and processing using an excavator-based stroke harvester for Pinus rigida and Quercus mongolica stands. To efficiently operate the stroke harvester, we developed a regression equation to estimate the productivities of felling and processing, felling, and processing operations,and we conducted sensitivity analysis of the operation costs using DBH and machine utilization. The felling and processing productivity was 6.53 and 4.02 m3/SMH for P. rigida a nd Q. mongolica, respectively, and the cost was 17,983 and 29,210 won/m3, respectively. The felling productivity for P. rigida a nd Q. mongolica wa s 40.9 and 23.0 m3/SMH, respectively, and the cost was 2,667 and 4,743 won/m3, respectively. The processing productivity for P. rigida and Q. mongolica was 8.25 and 7.75 m3/SMH, respectively, and the cost was 15,296 and 16,283 won/m3, respectively. In the developed regression equation, the DBH, traveling distance, and number of cuttings were found to be important factors (p<0.05). Therefore, it is necessary to construct a DB considering the various conditions and species associated with harvester operations, and further research is needed to increase the accuracy of predicting operation productivity and costs.

Reading the text of transformation from Seoljanggo Nori to dance - Regarding the transformation of Honam Udo Farmers' Music Lee Gyeonghwa Seoljanggo Dance - (설장고 놀이로부터 춤 변용으로의 텍스트 읽기 - 호남우도농악 이경화 설장고춤의 변용에 관해 -)

  • Kim, Ji-Won
    • (The) Research of the performance art and culture
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    • no.19
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    • pp.161-190
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    • 2009
  • This study presents matters of how to analyse the dance of artistic form on the course of transforming folk art to be separated from leisure to become the art form. In particular, the traditional art of dance in Korea has been of collective act like dureh, rather than of individual art, that it had to choose the repeated style of same form and rhythm. In this respect, before it can be said that the dance in its own form became more sophisticated and adopted the artistic segment in the time of modernisation, it is viewed that in the very heart of folk dance there was sufficient ability of artistic material to seek its own right. In this regard, the artistic transformation of seoljanggo nori into seoljanggo dance is an art form which is found in Korea, and expressing rhythm and playfulness is evident and sought attention. Therefore this study puts its importance in analysing how, in the aspect of the course of life of traditional arts, dance is formed in its own right and developed a form of art from fun entertainment. I have chosen, among them, seoljanggo, which used to be a form of fun entertainment and later transformed into a form of art on stage, in particular LeeGyeongh wa seoljanggo dance which maintains the style of Honam Udo farmers' music, and tried to read the text from it. It has resulted in that, Lee Gyeonghwa seoljanggo dance did a new try on tradition, in its development of expressing art through dance and onto more technical sophistication, found in the style of tune and choreography fused into its distinctive form. The art of traditional dance concerns here that seoljanggo has changed from agrarian entertainment to modern stage art, which shows how tradition can be adopted to the contemporary cultural life or to be reinvented to the needs of the aesthetic style that the current society consumes. Thus, it is necessary to think about its role in education and to represent cultural creativity from local developments.

Effects of Alginic Acid, Cellulose and Pectin Level on Bowel Function in Rats (알긴산과 셀룰로오스 및 펙틴 수준이 흰쥐의 대장기능에 미치는 영향)

  • 이형자
    • Journal of Nutrition and Health
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    • v.30 no.5
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    • pp.465-477
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    • 1997
  • The purpose of this article is to know the effects on bowel function of the kind of fiber and the amount of fiber in SD-rats. To do this experiment, we select of $\alpha$-cellulose as n insoluble cellulose source and alginic acid and pectin as soluble cellulose source. The rats diets contained callolose camcentrations of 1.0%, 3.6%, 6.0% and 10.0%. After that, we raised the SD-rats for 4weeks and measured the amount of food intake, body weight, the food effciency ratio, the length of liver and stomach the weight of the intestines, the transit time through the intestines, pH in feces, and the amount of bile acid and Ca, Mg, pp. 1) The amount of food intake was 15.75-31.00g/day. It was highest in the 10.0% cellulose group and the lowest in the 3.6% and 6.0% alginic acid group (p<0.05). The body weights of rats were 277.50-349.809. It was highest in the 1.0% pectin group and lowest in the 3.6% alginic acid group, 6.0% cellulose group, and 10.0% pectin group. It had differences according to the content fiber and the kind of dietary(p<0.01). The food efficiency ratio was (p<0.01). The higher the content of dietary fiber, the lower the calory and the food efficiency ratio. 2) Transit time was 446.0-775.0 minutes and it showed signidicant ifferences according to the content and kind of dietary fiber(p<0.01). It was long in the 1.0% cellulose group and 1.0% pectin group but short in the 10.0% alginic acid group. As the content of dietary fiber increased, the transit time through the intestines was shortened. The length of small intestine was 101.03-120.40cm and there were no difference cegardloss of the content and kind of fiber. The length of the large intestine was 20.92-25.42cm and there were significant differences according to the content and kind of the fiber. High-fiber diets resulted in increases in the length of the large intestine. 3) The weight of the liver was 8.68-10.96g and there were no differences according to the content and kind of fiber. The weight of stomach was 1.28-1.74g and there were no differences resulting from the kind of dietary fiber, but it was highest in the 10.0% alginic acid group. The weight of the small intestine was 5.52-8.04g with no difference resulting from to the kind of fiber. It was highest in the 10.0% the alginic acid group and lowest in the 1.0% alginic acid group(p<0.05). The weight of large intestine was 2.50-3.30g with no differences related to the kind of dietary fiber. It was heaviest in the 6.0% and 10.0% alginic acid groups and in the 10.0% pectin group with differences related to the content of fiber(p<0.05). 4) The pH of the feces was 5.82-6.86 according to the kind of dietary fiber, alginic acid group was high at 6.66, the cellulose group was 6.26. but the pectin group was low at 6.30. There were difference according to the content of fiber, but no consistency. The content of bile acid was 6.25-34.77umol per 1g of dry feces. According to the kind of dietary fiber, the alginic acid group was low at 12.91umol, cellulose group was 18.64umol and, the pectin group was the highest at 27.78umol(p<0.001). Based on the content of dietary fiber, alginic acid group was low at 1.0%, but high at 3.6% pectin group(p<0.001). 5) The amount of feces was 1.00-5.10g/day. The weight of rat feces was 2.23g/day in the alginic acid goup, 2.75g/day in the cellulose group, and 1.82g/day in the pectin group. According to the content of fiber, cellulose group was high at 10.0% but alginic acid group was 1.0%, and there were significant difference according to the dietary fiber. The more the content of fiber, the more increase the content of feces in alginic acid, cellulose and pectin group. The content of Ca in the feces was 80.10-207.82mg/1g of dry feces. In the dietary fiber, alginic acid group was 193.08mg, cellulose group was 87.5mg, pectin group was 138.16mg. In the content of fiber, alginic acid group was high at 1.0% and 3.6% but low at 10.0% of Pectin group. The content of Mg was 19.15-44.72mg/1g of dry feces. According to the kind of dietary fiber, alginic acid group was 35.33mg, cellulose group was 23.60mg, and pectin was 36.93mg. According to the content of fiber, pectin group was high at 1.0% and low at 10.0% of cellulose group. The content of P was 1.65-4.65mg/1g of dry feces. According to the kind of dietary fiber, alginic acid group 2.23mg/g dry feces, cellulose group was 2.29mg/g, pectin group wa 4.08mg/g dry feces. In the content of fiber, pectin group was high at 6.0% and low at 6.0% alginic acid group, but there were significant difference among the analysis value. The conetnt of Ca and MG was higher in soluble alginic acid group and pectin group than in insoluble cellulose group. The high the content of the dietary fiber, the lower the food efficiency ratio and the short the transit time through intestine with the increase of the length of large intestin as well as the higher level of the stomach, the small intestine and the large intestine. According to the content of the dietary fiber, the amount of the feces, Ca, Mg and P was increased but the length the small intestin, the weight of liver, pH of the feces and the amount of bile acid showed no differences and consistency.

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