• 한국미생물·생명공학회지
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• 제26권3호
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• pp.264-268
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• 1998

덱스트란수크라제는 Sucrose를 이용하여 덱스트란의 합성을 촉진하는데 sucrose이외에 다른 탄수화물이 효소 반응기 중에 존재하는 경우에는 Sucrose의 glucose를 이 탄수화물에 전달하는 반응을 촉진하여 새로운 구조의 산물을 생산한다. Leuconostoc mesenteroides B-742CB로 부터 얻은 덱스트란수크라제를 이용하여 플루란을 변형하고 그 조건을 최적화 하고자 했다. 수용성 변형 플루란은 이론적 수율의 57%(<$\pm$5)를 얻었다. 플루란 변형의 최적 조건으로는 pH 5.2, 28$^{\circ}C$ 에서 기질 0.37%(w/v)와 반응한 효소의 농도와 Sucrose농도가 각각 0.1 U/$m\ell$과 48mM일 때였다. 변형 플루란을 pullulanase, endodextranase로 처리하여 변형 전의 플루란과가수분해 상태를 비교 분석한 결과 변형전의 산물에 비해 이들 가수 분해 효소에 대해 더 저항성을 보였다. 변형 플루란을 methylation과 산가수분해 후 TLC한 결과 sucrose의 glucose가 플루란 glucose의 C3, C4, C6 위치의 free-OH group에 수식된 새로운 구조의 변형 플루란임을 확인하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.544-545
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• 2013

Recently, low-temperature atmospheric-pressure plasmas have been investigated [1,2] for biomedical applications and surface treatments. Experiments for improving hydrophilicity of stainless steel (SUS 304) plate with atmospheric microwave argon and H2O2 mixture plasma jet [3] were carried out and experimental measurements and plasma simulations were conducted for investigating the characteristics of plasma for the process. After 30 s of low power (under 10 W) and low temperature (under $50^{\circ}C$) plasma treatment, the water contact angle decreased rapidly to around $10^{\circ}$ from $75^{\circ}$ and was maintained under $30^{\circ}$ for a day (24 hours). The surface free energy, calculated from the contact angles, increased. The chemical properties of the surface were examined by X-ray Photoelectron Spectroscopy (XPS) and the surface morphology and roughness were examined by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) respectively. The characteristics of plasma sources with several frequencies were investigated by Optical Emission Spectroscopy (OES) measurement and one-dimensional Particle-in-Cell (PIC) simulation and zero-dimensional global simulation [4]. The relation between plasma components and the efficacy of the surface modification were discussed.

• 한국산업융합학회 논문집
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• 제23권3호
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• pp.491-498
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• 2020

Recently, In the production line of batteries, charge and discharge tests are essential to verify battery characteristics. In this case, the battery charging uses a unidirectional AC/DC converter capable of output voltage and current control, and the discharge uses a resistive load. Since this method consumes energy during discharge, it must be replaced with a bi-directional AC/DC converter system capable of charging and discharging. Although it is difficult to replace the connected inverter part of the bi-directional AC/DC converter system due to the high cost, the spread of the solar-connected inverter rapidly increases as the current solar supply business is activated, and thereby the solar-connected type Inverter prices are plunging. If it can be used as a power converter for battery discharge without program modification of the solar-powered inverter, it will have competition. In this paper, propose a new battery discharge system using a combination of a photovoltaic DC/DC simulator and photovoltaic PCS using a battery to be used as a power converter for battery discharge without program modification of a low-cost photovoltaic inverter. In addition, propose an optimal solar characteristic curve for the stable operation of PCS. The validity of the proposed system was verified using a 500[W] class solar DC/DC simulator and a solar PCS prototype.

• International Journal of Automotive Technology
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• 제7권5호
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• pp.571-577
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• 2006

This paper deals with dynamic tensile characteristics for the polypropylene used in an IP(Instrument Panel). The polypropylene is adopted in the dash board of a car, especially PAB(Passenger Air Bag) module. Its dynamic tensile characteristics are important because the PAB module undergoes high speed deformation during the airbag expansion. Since the operating temperature of a car varies from $-40^{\circ}C$ to $90^{\circ}C$ according to the specification, the dynamic tensile tests are performed at a low temperature($-30^{\circ}C$), the room temperature($21^{\circ}C$) and a high temperature($85^{\circ}C$). The tensile tests are carried out at strain rates of six intervals ranged from 0.001/sec to 100/sec in order to obtain the strain rate sensitivity. The flow stress decreases at the high temperature while the strain rate sensitivity increases. Tensile tests of polymers are rather tricky since polymer does not elongate uniformly right after the onset of yielding unlike the conventional steel. A new method is suggested to obtain the stress-strain curve accurately. A true stress-strain curve was estimated from modification of the nominal stress-strain curves obtained from the experiment. The modification was carried out with the help of an optimization scheme accompanied with finite element analysis of the tensile test with a special specimen. The optimization method provided excellent true stress-strain curves by enforcing the load response coincident with the experimental result. The material properties obtained from this paper will be useful to simulate the airbag expansion at the normal and harsh operating conditions.

• KSBB Journal
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• 제28권6호
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• pp.356-365
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• 2013

The novel Aloe gels were prepared with dewatering and impregnation by soaking (DIS) processing of Aloe vera leaf slice at four different temperatures (25, 35, 45 and $55^{\circ}C$), using dehydration solution of 40% (w/v) polyethylene glycol (PEG4000). The PEG-impregnation to Aloe vera leaf slice during DIS was observed depending on immersion temperature, and the PEG-impregnated Aloe vera gel (PEG-i-AVG) obtained was characterized using $^1H$ NMR, FT-IR, GPC, XRD and TGA. The PEG-i-AVG had the higher levels of Aloe bioactives (glucomannan and O-acetyl contents) and better quality indices by $^1H$ NMR and FT-IR spectroscopy than those of native Aloe gel. Also, the obtained Aloe gel maintained the bimodal patterns in higher molecular weight region by GPC indicating no degradation of polysaccharide from native Aloe gel. The result observed by SEM confirmed a surface modification by forming the porous structure, and TGA result exhibited better thermal stability than that of native Aloe gel. XRD result revealed that the crystalline structure in Aloe gel was led by incorporation of PEG. Significant decrease of %insolubility and high enhancement of water solubility index were observed, respectively, and highly ordered conformation such as a helix structure was also indicated by Congo red reaction. We concluded that the modification effect for enhancing function of native Aloe gel was successfully obtained by DIS process using PEG as a dehydrating agent. These results suggested that this DIS process had a high potential for developing a new minimally processed product from Aloe vera leaf.

• Journal of Biosystems Engineering
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• 제31권3호
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• pp.175-181
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• 2006

Nursery pig building is imperative to provide environmental conditions favorable to maintenance of piglet health and the efficiency of growth rate. To meet the ultimate goal, it is necessary to apply proper ventilation design and construction to a confinement livestock building. This study was conducted to investigate the performance of a modified ventilation system in terms of devised slot-inlet (modification I) and exhaust fan (modification II) to improve air change rate in a confined nursery pig building, with dimension of 5.9 m(W) ${\times}$ 12.6 m(L) ${\times}$ 2.2 m(H) in an Darby Genetic Station. The experiment was carried out in August, especially when the outdoor peak temperature were above $30^{\circ}C$ and the measured indoor environmental factors were temperature, air velocity, humidity and ammonia concentration which have been known to affect the piglet health and growth. There was no difference in indoor temperature between the original and modified ventilation systems, however the air velocity and ammonia concentration in confined nursery pig building with modified ventilation system were, in most cases, better performance than original ventilation system. Therefore, it was concluded that the slot-inlet system that kept indoor environmental factors pertinent and had an economic advantage, should be considered as a ventilation system for decreasing sensible heat from piglet in confined nursery pig building during extreme summer season.

• 전기화학회지
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• 제3권3호
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• pp.173-177
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• 2000

리튬 이차 전지의 성능은 부극으로 쓰이는 탄소재료의 표면의 미세 구조에 크게 의존한다. 본 연구에서는 이러한 표면 구조의 개질을 위해 유동상 화학증착법을 도입하여 금속 및 금속 산화물을 탄소재료 표면에 코팅하여 그 성능을 전기 화학적으로 평가하였다. 주석산화물을 코팅한 탄소 전극은 원래의 탄소 전극에 비해 용량의 상승을 나타내었으나 사이클이 진행됨에 따라 주석산화물이 코팅된 전지의 용량은 심각한 부피 변화에 의해 저하되어 사이클 수명이 감소되었다. 그러나, 부피 변화를 완화시켜주는 비활성 매트릭스 역할을 하는 구리를 주석 산화물 위에 코팅함으로 인해 부피 변화에 의한 용량 저하를 감소시킬 수 있었다.

• 생명과학회지
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• 제15권4호
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• pp.633-640
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• 2005

Bacillus pumilus TX703으로부터 xylanase를 정제한 후 효소의 활성부위를 조사하기 위하여 여러 가지 화학수식제를 사용하여 효소활성의 저해도를 측정하였다. 여러 가지 화학수식제 중에서 carbodiimide와 N-bromosuccinimide가 효소활성을 완전히 저 해시 켜 glutamic acid 또는 aspartic acid 잔기와 tryptophan 잔기가 효소의 활성부위에 관여하리라 추측되었다. 각각의 경우에 효소 실활은 수식제의 첨가농도에 따라 pseudo first-order kinetics 양식을 보여주었으며, car-bodiimide와 N-bromosuccinimide는 각각 비경쟁적 저해와 경쟁적 저해방식을 나타내었다. 기질첨가에 의한 효소활성 보호실험을 통하여 tryptophan 잔기가 기질결합부위라 판단되었다. 효소실활속도의 분석에 의해 효소활성에는 2개의 glutamic acid 또는 aspartic acid 잔기와 1개의 tryptophan 잔기가 관여하는 것으로 나타났다.

• Journal of Industrial and Engineering Chemistry
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• 제68권
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• pp.187-195
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• 2018

$Sr_{0.92}Y_{0.08}Ti_{1-x}Ni_xO_{3-{\delta}}$ (SYTN) was investigated in the presence of $H_2S$ containing fuels to assess the feasibility of employing oxide materials as alternative anodes. Aliovalent substitution of $Ni^{2+}$ into $Ti^{4+}$ increased the ionic conductivity of perovskite, leading to improved electrochemical performance of the SYTN anode. The maximum power densities were 32.4 and $45.3mW/cm^2$ in $H_2$ at $900^{\circ}C$ for the SYT anode and the SYTN anode, respectively. However, the maximum power densities in 300 ppm of $H_2S$ decreased by 7% and by 46% in the SYT and the SYTN anodes, respectively. To enhance the sulfur tolerance and to improve the electrochemical properties, the surface of SYTN anode was modified with samarium doped ceria (SDC) using the sol-gel coating method. For the SDC-modified SYTN anode, the cell performance was mostly recovered in the pure $H_2$ condition after 500-ppm $H_2S$ exposure in contrast to the irreversible cell performance degradation exhibited in the unmodified SYTN anode.

• Earthquakes and Structures
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• 제23권1호
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• pp.23-34
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• 2022

This paper presents experimental studies on reinforced concrete moment resisting frames that have engineered cementitious composite (ECC) in plastic hinge length (PHL) of beam/column members and beam-column joints. A two-story frame structure reduced by a 1:3 scale was further tested through a shake-table (seismic simulator) using multiple levels of simulated earthquake motions. One model conformed to all the ACI-318 requirements for IMRF, whereas the second model used lower-strength concrete in the beam/column members outside PHL. The acceleration time history of the 1994 Northridge earthquake was selected and scaled to multiple levels for shake-table testing. This study reports the observed damage mechanism, lateral strength-displacement capacity curve, and the computed response parameters for each model. The tests verified that nonlinearity remained confined to beam/column ends, i.e., member joint interface. Calculated response modification factors were 11.6 and 9.6 for the code-conforming and concrete strength deficient models. Results show that the RC-ECC frame's performance in design-based and maximum considered earthquakes; without exceeding maximum permissible drift under design-base earthquake motions and not triggering any unstable mode of damage/failure under maximum considered earthquakes. This research also indicates that the introduction of ECC in PHL of the beam/column members' detailing may be relaxed for the IMRF structures.