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Gene polymorphisms influencing yield, composition and technological properties of milk from Czech Simmental and Holstein cows

  • Citek, Jindrich;Brzakova, Michaela;Hanusova, Lenka;Hanus, Oto;Vecerek, Libor;Samkova, Eva;Krizova, Zuzana;Hostickova, Irena;Kavova, Tereza;Strakova, Karolina;Hasonova, Lucie
    • Animal Bioscience
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    • v.34 no.1
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    • pp.2-11
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    • 2021
  • Objective: The aim of the study was to evaluate the influence of polymorphic loci and other factors on milk performance and the technological properties of milk. Methods: The analysis was performed on Simmental and Holstein cows in field conditions (n = 748). Milk yield in kg, fat and protein percentage and yield were evaluated. Technological properties were evaluated by milk fermentation ability, renneting, and an alcohol test. Polymorphisms in the acyl-CoA diacylgycerol transferase 1 (DGAT1), leptin (LEP), fatty acid synthase (FASN), stearoyl CoA desaturase 1 (SCD1), casein beta (CSN2), casein kappa (CSN3), and lactoglobulin beta genes were genotyped, and association analysis was performed. Results: The DGAT1 AA genotype was associated with higher milk, protein and fat yields (p<0.05). The MM genotype in the LEP gene was associated with a lower protein percentage and the W allele with a higher protein percentage (p<0.05). In cows with the FASN GG genotype, the protein percentage was higher, but the A allele was associated with higher milk, protein and fat yields than the G allele. The TT genotype in SCD1 was associated with the lowest milk, protein and fat yields and with the highest milk protein percentage (p<0.01). The T allele had higher values than the C allele (p<0.05) except for fat percentage. The genotype CSN3 AA was associated with a significantly heightened milk yield; BB was associated with a high protein percentage. The effect of the alleles on the technological properties was not significant. The CSN2 BB genotype was associated with the best alcohol test (p<0.01), and the renneting order was inverse. Milk from cows with the CSN2 A1A1 genotype was best in the milk fermentation ability. CSN3 significantly affected the technological properties. Conclusion: The findings revealed the potential of some polymorphic loci for use in dairy cattle breeding and for the management of milk quality. In field research, the pivotal role of farms in milk yield, composition and technological properties was confirmed.

FREE-FLOATING PLANETS, THE EINSTEIN DESERT, AND 'OUMUAMUA

  • Gould, Andrew;Jung, Youn Kil;Hwang, Kyu-Ha;Dong, Subo;Albrow, Michael D.;Chung, Sun-Ju;Han, Cheongho;Ryu, Yoon-Hyun;Shin, In-Gu;Shvartzvald, Yossi;Yang, Hongjing;Yee, Jennifer C.;Zang, Weicheng;Cha, Sang-Mok;Kim, Dong-Jin;Kim, Seung-Lee;Lee, Chung-Uk;Lee, Dong-Joo;Lee, Yongseok;Park, Byeong-Gon;Pogge, Richard W.
    • Journal of The Korean Astronomical Society
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    • v.55 no.5
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    • pp.173-194
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    • 2022
  • We complete the survey for finite-source/point-lens (FSPL) giant-source events in 2016-2019 KMTNet microlensing data. The 30 FSPL events show a clear gap in Einstein radius, 9 𝜇as < 𝜃E < 26 𝜇as, which is consistent with the gap in Einstein timescales near tE ~ 0.5 days found by Mróz et al. (2017) in an independent sample of point-source/point-lens (PSPL) events. We demonstrate that the two surveys are consistent. We estimate that the 4 events below this gap are due to a power-law distribution of free-floating planet candidates (FFPs) dNFFP/d log M = (0.4 ± 0.2) (M/38 M)-p/star, with 0.9 ≲ p ≲ 1.2. There are substantially more FFPs than known bound planets, implying that the bound planet power-law index 𝛾 = 0.6 is likely shaped by the ejection process at least as much as by formation. The mass density per decade of FFPs in the Solar neighborhood is of the same order as that of 'Oumuamua-like objects. In particular, if we assume that 'Oumuamua is part of the same process that ejected the FFPs to very wide or unbound orbits, the power-law index is p = 0.89 ± 0.06. If the Solar System's endowment of Neptune-mass objects in Neptune-like orbits is typical, which is consistent with the results of Poleski et al. (2021), then these could account for a substantial fraction of the FFPs in the Neptune-mass range.

Sinomonas terrae sp. nov., Isolated from an Agricultural Soil

  • Hyosun Lee;Ji Yeon Han;Dong-Uk Kim
    • Journal of Microbiology and Biotechnology
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    • v.33 no.7
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    • pp.909-914
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    • 2023
  • While searching for the bacteria which are responsible for degradation of pesticide in soybean field soil, a novel bacterial strain, designated 5-5T, was isolated. The cells of the strain were Gram-staining-positive, aerobic and non-motile rods. Growth occurred at 10-42℃ (optimum, 30℃), pH 5.5-9.0 (optimum, pH 7.0-7.5), and 0-2% (w/v) NaCl (optimum, 1%). The predominant fatty acids were C15:0 anteiso, C17:0 anteiso, and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The predominant menaquinone was MK-9 (H2). Diphosphatidylglycerol, glycolipids, phosphatidylinositol, and phosphatidylglycerol were the major polar lipids. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 5-5T is a member of the genus Sinomonas and its closest relative is Sinomonas humi MUSC 117T, sharing a genetic similarity of 98.4%. The draft genome of strain 5-5T was 4,727,205 bp long with an N50 contig of 4,464,284 bp. Genomic DNA G+C content of strain 5-5T was68.0 mol%. The average nucleotide identity (ANI) values between strain 5-5T and its closest strains S. humi MUSC 117T and S. susongensis A31T were 87.0, and 84.3 % respectively. In silico DNA-DNA hybridization values between strain 5-5T and its closest strains S. humi MUSC 117T and S. susongensis A31T were 32.5% and 27.9% respectively. Based on the ANI and in silico DNA-DNA hybridization analyses, the 5-5T strain was considered as novel species belonging to the genus Sinomonas. On the basis of the results from phenotypic, genotypic and chemotaxonomic analyses, strain 5-5T represents a novel speciesof the genus Sinomonas, for which the name Sinomonas terrae sp. nov. is proposed. The type strain is 5-5T (=KCTC 49650T =NBRC 115790T).

Evaluation of Nutrient Condition and Fruit Quality of 'Campbell Early' Grapevines in Hwaseong Area (화성지역 '캠벨얼리' 포도원의 수체영양과 과실품질의 평가)

  • Lee, Y.C.;Moon, B.W.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.5 no.1
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    • pp.28-35
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    • 2003
  • This study was conducted to investigate the nutrition status, growth and fruit quality of 'Campbell Early' grapes in Hwaseong area. Organic matter content of 3 vineyard soils was significantly lower than standard value. P2O5 and K contents of Y vineyard and P2O5 of C vineyard were very high. But there were no significant differences in contents of Ca, Mg and CEC. Compared to the standard value. T-N, Ca and Mg contents of petiole were adequate. P2O5 contents of Y and C vineyard and K contents of Y vineyard were significantly higher than standard value. Berry firmness, color and bloom occurrence on fruit skin showed no differences among 3 vineyard. Soluble solids content poorly, yield per tree and goods fruit of C vineyard decreased significantly compared to the fruit in Y and H vineyards. Russetted fruit of Y vineyard, colored fruit of H vineyard, and unfertilized fruit, cracked fruit, and bitter rot were observed frequently.

Nature of the Interfacial Regions in the Antiferromagnetically-coupled Fe/Si Multilayered Films

  • Moon, J.C.;Y.V. Kudryavtsev;J.Y.Rhee;Kim, K.W.;Lee, Y.P.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.174-174
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    • 2000
  • A strong antiferromagnetic coupling in Fe/Si multilayered films (MLF) had been recently discovered and much consideration has been given to whether the coupling in the Fe/Si MLF system has the same origin as the metal/metal MLF. Nevertheless, the nature of the interfacial ron silicide is still controversial. On one hand, a metal/ semiconductor structure was suggested with a narrow band-gap semiconducting $\varepsilon$-FeSi spacer that mediates the coupling. However, some features show that the nature of coupling can be well understood in terms of the conventional metal/metal multilayered system. It is well known that both magneto-optical (MO) and optical properties of a metal depend strongly on their electronic structure that is also correlated with the atomic and chemical ordering. In this study, the nature of the interfacial regions is the Fe/Si multilayers has been investigated by the experimental and computer-simulated MO and optical spectroscopies. The Fe/Si MLF were prepared by rf-sputtering onto glass substrates at room temperature with the number of repetition N=50. The thickness of Fe sublayer was fixed at 3.0nm while the Si sublayer thickness was varied from 1.0 to 2.0 nm. The topmost layer of all the Fe/Si MLF is Fe. In order to carry out the computer simulations, the information on the MO and optical parameters of the materials that may constitute a real multilayered structure should be known in advance. For this purpose, we also prepared Fe, Si, FeSi2 and FeSi samples. The structural characterization of Fe/Si MLF was performed by low- and high -angle x-ray diffraction with a Cu-K$\alpha$ radiation and by transmission electron microscopy. A bulk $\varepsilon$-FeSi was also investigated. The MO and optical properties were measured at room temperature in the 1.0-4.7 eV energy range. The theoretical simulations of MO and optical properties for the Fe/Si MLF were performed by solving exactly a multireflection problem using the scattering matrix approach assuming various stoichiometries of a nonmagnetic spacer separating the antiferromagnetically coupled Fe layers. The simulated spectra of a model structure of FeSi2 or $\varepsilon$-FeSi as the spacer turned out to fail in explaining the experimental spectra of the Fe/Si MLF in both intensity and shape. Thus, the decisive disagreement between experimental and simulated MO and optical properties ruled out the hypothesis of FeSi2 and $\varepsilon$-FeSi as the nonmagnetic spacer. By supposing the spontaneous formation of a metallic ζ-FeSi, a reasonable agreement between experimental and simulated MO and optical spectra was obtained.

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Improvement of lower hybrid current drive systems for high-power and long-pulse operation on EAST

  • M. Wang;L. Liu;L.M. Zhao;M.H. Li ;W.D. Ma;H.C. Hu ;Z.G. Wu;J.Q. Feng ;Y. Yang ;L. Zhu ;M. Chen ;T.A. Zhou;H. Jia;J. Zhang ;L. Cao ;L. Zhang ;R.R. Liang;B.J. Ding ;X.J. Zhang ;J.F. Shan;F.K. Liu ;A. Ekedahl ;M. Goniche ;J. Hillairet;L. Delpech
    • Nuclear Engineering and Technology
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    • v.54 no.11
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    • pp.4102-4110
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    • 2022
  • Aiming at high-power and long-pulse operation up to 1000 s, some improvements have been made for both 2.45 GHz and 4.6 GHz lower hybrid (LH) systems during the recent 5 years. At first, the guard limiters of the LH antennas with graphite tiles were upgraded to tungsten, the most promising material for plasma facing components in nuclear fusion devices. These new guard limiters can operate at a peak power density of 12.9 MW/m2. Strong hot spots were usually observed on the old graphite limiters when 4.6 GHz system operated with power >2.0 MW [B. N. Wan et al., Nucl. Fusion 57 (2017) 102019], leading to a reduction of the maximum power capability. With the new limiters, 4.6 GHz LH system, the main current drive (CD) and electron heating tool for EAST, can be operated with power >2.5 MW routinely. Long-pulse operation up to 100 s with 4.6 GHz LH power of 2.4 MW was achieved in 2021 and the maximal temperature on the guard limiters measured by an infrared (IR) camera was about 540 ℃, much below the permissible value of tungsten material (~1200 ℃). A discharge with a duration of 1056 s was achieved and the 4.6 GHz LH energy injected into the plasma was up to 1.05 GJ. Secondly, the fully-active-multijunction (FAM) launcher of 2.45 GHz system was upgraded to a passive-active-multijunction (PAM), for which the density of optimum coupling was relatively low (below the cut-off value). Good coupling with reflection coefficient ~3% has been achieved with plasma-antenna distance up to 11 cm for the new PAM. Finally, in order to eliminate the effect of ion cyclotron range of frequencies (ICRF) wave on 4.6 GHz LH wave coupling, the location of the ICRF launcher was changed to a port that is located 157.5° toroidally from the 4.6 GHz LH system and is not magnetically connected.

Effect of treadmill exercise on apoptosis in the retinas of streptozotocin-induced diabetic rats (트레드밀 운동이 streptozotocin에 의해 유발된 당뇨 쥐의 망막 신경세포 사멸에 미치는 영향)

  • Kim, D.Y.;Jung, S.Y.;Kim, T.W.;Sung, Y.H.
    • Exercise Science
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    • v.21 no.3
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    • pp.289-298
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    • 2012
  • In the present study, we investigated the effect of treadmill exercise on apoptotic neuronal cell death in the retinas of streptozotocin-induced diabetic rats. Twenty-eight male Sprague-Dawley rats were used for this study. The animals were divided into four groups(n = 7 in each group):(1) control group, (2) exercise group, (3) diabetes-induced group, (4) diabetes-induced and exercise group. Diabetes mellitus(DM) was induced by intraperitoneal injection of streptozotocin. The rats in the exercise groups were forced to run on the treadmill for 30 minutes once a day, five times per a week, during 12 weeks. In this study, a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay and western blot for the expressions of caspase-3, cytochrome c, Bax, and Bcl-2 in the retinas were conducted for the detection of apoptotic retinal cell death. The present results showed that the number of TUNEL-positive cells was increased in the retinas of the diabetic rats, whereas treadmill exercise suppressed this number. The expressions of pro-apoptotic factors caspase-3, cytochrome c, and Bax were enhanced and the expressions of anti-apoptotic factor Bcl-2 was decreased in the retinas of the diabetic rats. In contrast, treadmill exercise suppressed the expressions of caspase-3, cytochrome c, and Bax and increased the expression of Bcl-2. The present study demonstrated that treadmill exercise suppressed diabetes-induced apoptotic neuronal cell death in the retinas. Based on the present results, treadmill exercise may be effective therapeutic strategy for the alleviating complications of diabetes patients.

The Effect of Imagined Contact on Attraction to Unattractive Women (상상 접촉이 이성에 대한 호감 및 매력 지각에 미치는 영향)

  • Yong Hoe Heo;Sean S. Kim;Sun W. Park
    • Korean Journal of Culture and Social Issue
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    • v.22 no.2
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    • pp.203-222
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    • 2016
  • Previous research has demonstrated that imagined contact is useful to reducing prejudice against minorities (e.g. Turner & Crisp, 2010). In this research, we investigated whether imagined contact increases attraction to physically unattractive women, and whether this effect is moderated by perceiver's physical attractiveness and appearance orientation in romantic relationships. Single male participants (n = 98) responded to items assessing their physical attractiveness and appearance orientation, and then were randomly assigned to one of the two conditions. In the imagined contact condition, participants were told to imagine communicating with a physically unattractive woman and come up with some interesting things about her. In the control condition, participants were told to simply imagine a physically unattractive woman. Next, participants saw a picture of a physically unattractive woman and rated her likeability, perceived attractiveness, and willingness to ask her out, which were averaged to form the composite measure of attraction. We found a significant main effect of imagined contact, as expected. This main effect was moderated by appearance orientation, but not by perceivers' physical attractiveness. The effect of imagined contact was significant among those low in appearance orientation, but not among those high in appearance orientation.

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Explicit and Implicit Attitudes Toward Homosexuals (동성애자에 대한 외현적 및 암묵적 태도)

  • Lee Hyun Yoon ;Min Hee Yoo ;Jae Hee Ryu ;Sun W. Park
    • Korean Journal of Culture and Social Issue
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    • v.22 no.3
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    • pp.343-362
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    • 2016
  • Despite recent social movements to protect homosexuals' rights in Korea, psychological research investigating attitudes toward homosexuals has been largely ignored. The present study examined Koreans' explicit and implicit attitudes toward homosexuals and how openness is related to them. College students (N = 56) responded to questionnaires assessing explicit attitudes toward homosexuals and openness, one of the five factors of personality. They then took an Implicit Association Test designed to assess implicit attitudes toward homosexuals. We found that participants in general had more negative explicit attitudes toward gay men than lesbians. Implicit prejudice against gay men was also higher than lesbians. There was no participant sex difference in implicit attitudes toward gay men. However, male participants had more negative implicit attitudes toward lesbians than female participants did; in fact, females' implicit attitudes toward lesbians were not biased. While openness was negatively related only to explicit prejudice, values, one of the facets of openness, was negatively related to both explicit and implicit prejudice. This was the first study in Korea that investigated both explicit and implicit attitudes toward gay men and lesbians.

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Evaluation of Reproductive Performance in Heifers with Different Energy Intake (육성기 에너지 급여 차이에 따른 젖소 번식능력 평가)

  • Lee, W.Y.;Kim, J.N.;Cho, K.H.;Yeo, J.M.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.2
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    • pp.49-56
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    • 2018
  • Recently, attempts have been made by dairy farms to shorten the time until the first estrus by feeding animals a high-energy diet during the heifer period. Energy consumption during the heifer period has been shown to be related to milk yield and reproductive performance. The present study was conducted to analyze the relationship between reproductive performance and energy intake in heifers. There were no differences in height or body weight between animals that were fed the Korean standard diet versus a high energy diet (110% of the Korean standard). However, there was a difference in the timing of the first estrus: 329.3 days under normal energy conditions versus 311 days under high energy conditions. There was no difference in the number of artificial insemination per gestation, but both the rate of female calf production and the weight of individual female calves increased in response to the high energy diet. Based on these results, it may be predicted that the reproductive performance of dairy cows will increase when the animals are fed a high energy diet (110% of the Korean standard). These results demonstrate a correlation between the amount of energy consumed and reproductive performance, and will be of great value to the dairy farming industry.