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Bipolar Heterothallism, a Principal Mating System of Cordyceps militaris In Vitro

  • Shrestha Bhushan;Kim Ho Kyung;Sung Gi Ho;Spatafora Joseph W.;Sung Jae Mo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.440-446
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    • 2004
  • Interest in in vitro study of entomopathogenic fungi, including Cordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. Among Cordyceps species, in vitro stromata of C militaris has been successfully produced and cultivated for industrial purposes. However, genetic study on in vitro stromata formation of C militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation of C militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, mono­ascospore cultures without combination produced either no stromata or only abnormal non­perithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains of C militaris. These observations indicated that C militaris behaves as a bipolar het­erothallic fungus and requires two mating compatible strains in order to produce regular club­shaped perithecial stromata, a fundamental requirement for its industrial cultivation.

Influences of the Irradiation of Intense Pulsed ion Beam (IPIB) on the Surface of Ni$_3$Al Base Alloy IC6

  • Le, X.Y.;Yan, S.;Zhao, W.J.;Han, B.H.;Wang, Y.G.;Xue, J.M.;Zhang, H.T.
    • Journal of Korean Vacuum Science & Technology
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    • v.6 no.2
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    • pp.92-96
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    • 2002
  • In this paper, we treated the Ni$_3$Al based alloy samples with intense pulsed ion beams (IPIB) at the beam parameters of 250KV acceleration voltage, 100 - 200 A/cm$^2$ current density and 60 u pulse duration. We simulated the thermal-mechanical process near the surface of Ni$_3$Al based alloy with our STEIPIB codes. The surface morphology and the cross-section microstructures of samples were observed with SEM, the composition of the sample surface layer was determined by X-ray Energy Dispersive Spectrometry (XEDS) and the microstructure on the surface was observed by Transmission Electron Microscope (TEM). The results show that heating rate increases with the current density of IPIB and cooling rate reached highest value less than 150 A/cm$^2$. The irradiation of IPIB induced the segregation of Mo and adequate beam parameter can improve anti-oxidation properly of IC6 alloy. Some craters come from extraneous debris and liquid droplets, and some maybe due to the melting of the intersection region of interphase. Increasing the pulse number enlarges average size of craters and decreases number density of craters.

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Study on the Metal Ore Deposits of Gyeongsang buk-do Area (경상북도(慶尙北道) 일원(一圓)에 부존(賦存)하고 있는 금속지하자원(金屬地下資源)의 지질광상학적(地質鑛床學的) 연구(硏究))

  • Kim, Y.K.;Lee, J.Y.;Kim, S.W.;Koh, I.S.
    • Economic and Environmental Geology
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    • v.9 no.3
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    • pp.143-156
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    • 1976
  • The Cretaceous metal ore deposits in the Gyeongsang basin of Gyeongsangbuk-do are characterized by the formation of metallogenic provinces which show zonal distribution pattern around Yeonil province where pneumatolytic type is dominated and hydrothermal type are distributed in the order of decreasing temperature type outward. Some Cretaceous granitic rocks include zoned alkali feldspars which reflect rapid variation of $H_2O$ during emplacement and crystallization of the water-saturated granitic magma. The ore deposits are considered to be originated from upward transportation of ore solution from the excess of water exhausted from uprising magma, which seems to be intimately related to the fact that the majority of the ore deposits in Daegu area are cummulated around the granites including zoned alkali feldspars. In order to collect geochemical data necessary for geochemical exploration in the study area, certain trace elements were chosen as pathfinders from monzonite and soil in the vicinity of Dalsung Tungsten Mine by studying the dispersion patterns of trace elements: Ba and Sr show trends to decrease toward ore deposit while Cu, Pb, and Mo increase. Around mining area there are distributed apparently Equisetum arvense Linne and Mentha sachinensis Kudo which may be used as index plants. In the viewpoint of geologic structure, the trends of the ore veins in contact aureole around the Palgongsan granite body correspond with the pre- and syn- plutonism joint pattern in hornfels in the area.

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Aerodynanamic design and performance analysis of a 5kW HAWT rotor blades (5Kw급 수평축 풍력 터빈 로터블레이드의 공력 설게 및 성능예측)

  • Kim, Mun-Oh;Kim, Bum-Suk;Mo, Jang-Ho;Lee, Young-Ho
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.182.1-182.1
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    • 2010
  • 현재 전 세계적으로 가장 널리 개발하고 보급되어지고 있는 풍력산업의 시장 규모는 매년 확대되고 있다. 특히 소형 풍력발전 시스템은 낙도 등의 전력 공급이 어려운 지역에 경제성 있는 전력 보급을 가능하게 한다. 국내의 미전화 지역과 일반 가정에서 풍력 에너지 자원을 적극 활용 개발하기 위해서 보다 우수한 성능의 풍력발전기용 블레이드를 설계하고자, 공기역학적인 최적설계에 대해 연구함으로써 추후 보급형 풍력발전 시스템의 개발에 필요한 설계 기술을 확립하고자한다. 본 연구는 설계된 블레이드의 유동해석 및 성능예측을 위하여 경제적으로 많은 지원이 필요한 대규모 풍동실험이 아닌 상용 CFD를 사용하여 보다 효율적으로 우수한 성능을 가지는 풍력 터빈을 설계함에 있다. Reynolds Averaged Navier-Stokes 방정식에 기반을 둔 CFD의 경우 이론적으로 명확한 해석이 가능하고, 실제 터빈의 운전 환경과 동일한 다양한 물리적 변수를 입력 데이터로서 활용할 수 있는 장점이 있기 때문에 풍력 터빈의 설계 과정에서 반영된 미소한 블레이드 형상변화 및 운전 조건의 변화에 따른 유동장의 변화 및 풍력터빈 성능을 정확히 예측할 수 있는 장점을 가지고 있다.

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Channel geometry-dependent characteristics in silicon nano-ribbon and nanowire FET for sensing applications

  • Choe, Chang-Yong;Hwang, Min-Yeong;Kim, Sang-Sik;Gu, Sang-Mo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.33-33
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    • 2009
  • Silicon nano-structures have great potential in bionic sensor applications. Atomic force microscopy (AFM) anodic oxidation have many advantages for the nanostructure fabrication, such as simple process in atmosphere at room temperature, compatibility with conventional Si process. In this work, we fabricated simple FET structures with channel width W~ 10nm (nanowire) and $1{\mu}m$ (nano-ribbon) on ~10, 20 and 100nm-thinned silicon-on-insulator (SOI) wafers in order to investigate the surface effect on the transport characteristics of nano-channel. For further quantitative analysis, we carried out the 2D numerical simulations to investigate the effect of channel surface states on the carrier distribution behavior inside the channel. The simulated 2D cross-sectional structures of fabricated devices had channel heights of H ~ 10, 20, and 100nm, widths of L ~ $1{\mu}m$ and 10nm respectively, where we simultaneously varied the channel surface charge density from $1{\times}10^{-9}$ to $1{\times}10^{-7}C/cm2$. It has been shown that the side-wall charge of nanowire channel mainly affect the I-V characteristics and this was confirmed by the 2D numerical simulations.

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저온공정에서 제작한 ZnO:Al 박막의 특성 분석

  • Jung, Yu-Sup;Kim, Sang-Mo;Hong, Jung-Soo;Son, In-Hwan;Kim, Kyung-Hwan
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.201-202
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    • 2009
  • ZnO:Al transparent conductive films for solar cells were deposited on the glass substrates at room temperature by facing target sputtering (FTS) method. The sputtering targets were 100 mm diameter disks of 2w.t..%. AZO and Zn metal. ZnO:Al thin films were deposited as a function film thickness. A base pressure was $2{\times}10^{-6}$torr, and a working pressure was 1mTorr. The properties of thin films on the structural, electrical and optical properties of the deposited films were investigated using a four-point probe (Chang-min), an X-ray diffraction (Rigaku), a Hall Effect measurement (Ecopia), an UV/VIS spectrometer (HP) and a $\alpha$-step (Tencor). The lowest resistivity of film was $5.67{\times}10^{-4}[{\Omega}-cm]$ at 500nm. The average transmittance of over 80% was seen in the visible range.

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A simulation study on the figure of merit optimization of a 1200V 4H-SiC DMOSFET (1200V급 4H-SiC DMOSFET 성능지수 최적화 설계 시뮬레이션)

  • Choi, Chang-Yong;Kang, Min-Suk;Bang, Wook;Kim, Sang-Chul;Kim, Nam-Kyun;Koo, Sang-Mo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.63-63
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    • 2009
  • In this work, we demonstrate 800V 4H-SiC power DMOSFETs with several structural alterations to observe static DC characteristics, such as a threshold voltage ($V_{TH}$) and a figure of merit ($V_B^2/R_{SP,ON}$). To optimize the static DC characteristics, we consider four design parameters; (a) the doping concentration ($N_{CSL}$) of current spreading layer (CSL) beneath the p-base region, (b) the thickness of p-base ($t_{BASE}$), (c) the doping concentration ($N_J$) and width ($W_J$) of a JFET region, (d) the doping concentration ($N_{EPI}$) and thickness ($t_{EPI}$) of epi-layer. Design parameters are optimized using 2D numerical simulations and the 4H-SiC DMOSFET structure results in high figure of merit ($V_B^2/R_{SP,ON}$>~$340MW/cm^2$) for a power MOSFET in $V_B{\sim}1200V$ range.

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팽나무버섯(Flammulina velelutipes)균의 액체배양 생장조건 규명(Growth condition of Flammulina velutipes in Liquid culture)

  • Hong, Seong-Jun;Lee, Won-Ho;Park, Gi-Beom;Park, Gi-Byeong;Seong, Jae-Mo
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.355-358
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    • 2001
  • Selection of optima] nutrient sources and cultural methods for liquid spawn culture of Flammulina velutipes were carried out. The optimal temperature and pH for mycelial growth of F. velutipes were $20^{\circ}C$ and 6.0 to 7.5. respectively. In the 250ml ${Delta}$-flask culture. the amount of inoculum and culture period for the optimal mycelial growth of F. velutipes were 3 mycelial disks(diametcr 6mm) and 6 days, respectively. For the mass production of submerged culture. the optimal inoculum amount and aeration rate of F. velutipes were 5%(inoculum vol/medium vol.) and l.0vvm(vol of air/vol. of medium/min), respectively.

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Calculation of the Number of Channels and Bandwidth for Common Frequency Band Based on Queueing Theory (대기행렬이론에 의한 공유주파수대역의 적정 채널수 및 대역폭 산출)

  • Lee, Gwang-Hui;Gang, Sang-Gi;Hwang, Taek-Jin;Hong, Heon-Jin;Cha, Jae-Sang;Lee, Il-Gyu;Yang, Chung-Mo;Kim, Seong-Gwon
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2007.04a
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    • pp.473-476
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    • 2007
  • 본 논문에서는 LBT(Listen Before Talk) 방식을 사용하는 ZigBee와 FH(Frequency Hopping) 방식을 사용하는 DCP, RFID, Bluetooth 등의 소출력 무선기기가 공유주파수대역에 공존할 경우 요구되는 적정 채널수를 산출하고 전체 공유주파수대역폭 산출법을 제시하였다. 한정된 주파수 대역에서 LBT 및 FH 방식을 사용하는 총 User 수가 포용되는 공유주파수대역폭 산출은 중요한 작업이다. 소출력 무선기기 시스템의 간섭 회피 기술로 사용되는 FH 방식과 LBT 방식에 대기행렬이론(Queueing Theory)을 적용하였으며, 주위의 전파환경을 감지하여 유휴 주파수대역을 찾아 데이터전송을 시도하는 LBT 방식은 random하게 주파수채널을 이동하며 통신을 시도하는 FH 방식과는 구별된다. 채널수 별 User의 통신시도 시간간격을 통계적으로 처리하여 Throughput을 분석한 결과, Throughput 70% 조건에서 FH 방식과 LBT 방식을 사용하는 250mW 소출력 무선기기들이 공존하는 공유주파수대역의 적정 채널수는 30개를 가지며, 전체 공유주파수대역폭은 채널수에 채널당 대역폭의 곱으로 산출이 가능하다.

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Development, Validation, and Application of a Portable SPR Biosensor for the Direct Detection of Insecticide Residues

  • Yang, Gil-Mo;Cho, Nam-Hong
    • Food Science and Biotechnology
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    • v.17 no.5
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    • pp.1038-1046
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    • 2008
  • This study was carried out to develop a small-sized biosensor based on surface plasmon resonance (SPR) for the rapid identification of insecticide residues for food safety. The SPR biosensor module consists of a single 770 nm-light emitting diodes (LED) light source, several optical lenses for transferring light, a hemisphere sensor chip, photo detector, A/D converter, power source, and software for signal processing using a computer. Except for the computer, the size and weight of the sensor module are 150 (L)$\times$70 (W)$\times$120 (H) mm and 828 g, respectively. Validation and application procedures were designed to assess refractive index analysis, affinity properties, sensitivity, linearity, limits of detection, and robustness which includes an analysis of baseline stability and reproducibility of ligand immobilization using carbamate (carbofuran and carbaryl) and organophosphate (cadusafos, ethoprofos, and chlorpyrifos) insecticide residues. With direct binding analysis, insecticide residues were detected at less than the minimum 0.01 ppm and analyzed in less than 100 sec with a good linear relationship. Based on these results, we find that the binding interaction with active target groups in enzymes using the miniaturized SPR biosensor could detect low concentrations which satisfy the maximum residue limits for pesticide tolerance in Korea, Japan, and the USA.