• Journal of Nutrition and Health
• /
• 제30권9호
• /
• pp.1061-1066
• /
• 1997

This study was undertaken to evaluate the effect of 4 weeks of $\alpha$-tocopherol(800 I.U./d) supplementation on oxidant stress of eleven female aerobic -majoring students during rest and exercise. Changes in the activity of the antioxidant enzyme glutathione peroxidase were also studied. Serum $\alpha$-tocopherol concentration was significantly increased with vitamin E supplementation(710.1$\pm$113.8$\mu\textrm{g}$/dl vs. 1,485,8$\pm$105.2$\mu\textrm{g}$/dl). In addition, serum MDA concentration, an index of lipid peroxidation, significantly decreased after vitamin E supplementation. However, MDA values after exercise increased to pre-supplementation levels. Serum glutathione peroxidase activity significantly increased with vitamin E supplementation. The enzyme activity showed a trend toward decrease after exercise. Serum cholesterol values were not significantly affected by vitamin E supplementation. However, serum triglycerides significantly increased after supplementation against oxidative stress during resting periods. These supplements appraently work by decreasing lipid peroxidation and increasing glutathione peroxidase activity. However, vitamin E supplementation did not prevent exercise-induced increases in lipid peroxidation.

• Journal of Nutrition and Health
• /
• 제33권2호
• /
• pp.167-178
• /
• 2000

The effect of oral vitamin e (800IU/day) and C (500mg/day) supplementation for 10 days and/or smoking cessation for 5 days on oxidative damage to the red blood cells (RBC) of male smokers (22.2$\pm$0.2 years old) was studied. RBC were tested for their ability to protect against smoking-induced oxidative damage by measuring heme proteins (carboxyhemoglobin, hemoglobin, methemoglobin, oxyhemoglobin), hemolysis and thiobarbiturinc acid reactive substances (TBARS). Plasma levels of vitamin c, A, E, $\beta$-catotene, total cholesterol, glutamic pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) were also analyzed. In experiment one, a comparison was made of heme proteins and lipid damage to RBC, plasma antioxidant status (indexed by plasma levels of vitamin C, E, A and $\beta$-carotene) between smokers(n=56) and non-smokers (n=16). No differences were found in plasma antioxidant status, heme protein damage and TBARS concentration of RBC. In experiment two, 46 fasting male smokers from experiment one were divided into 4 groups. The groups were smoking with placebo group(SP, n=14), smoking cessation with vitamins supplementatin group (SV, n=13), smoking cessation with placebo group (NSP, n=9) and smoking cessation with vitamins supplementation group (NSV, n=10). After supplementing antioxidant vitamins, significant increases were seen in plasma vitamins supplementation group (NSV, n=10). After supplementing antioxidant vitamins, significant increases were seen plasma vitamin C (p<0.05) and vitamin E levels (p<0.05). The plasma vitamin E level was highest in the NSV group. Vitmain E and C supplementation provided some protection against heme proteins and lipid damage by lowering methemoglobin, hemolysis and TBARS concentration of RBC. Smoking cessation significantly decreased TBARS of RBC and plasma total cholesterol concentration. Supplementing vitamin E and C with smoking cessation considerably lowered plasma total cholesterol. These results point to a special association among smoking, oxidative damage and plasma antioxidant vitamin status. They indicate that increases in plasma antioxidant status can be detected after the supplementation of vitamin C and E and that smoking cessation had an additional effect on plasma vitamin E level. The present data suggest that improved antioxidant status induced by antioxidant supplementation or smoking cessation may help prevent oxidative damage in smokers.

• 한국식품영양과학회지
• /
• 제22권6호
• /
• pp.664-672
• /
• 1993

알코올의 섭취가 Se가 vitamin E 수준을 달리한 흰주의 체내 Se 수준 및 심장, 간세포 형태에 미치는 영향을 살펴보기 위하여 평균 체중이 58~62g인 Sprague-Dawley계의 젖을 갖뗀 숫쥐 72마리를 12군으로 나누어 Se의 수준을 식이 kg당 0mg, 0.4mg,10mg으로 달리하고 vitamin E는 식이 Kg당 0mg, 150mg으로 하여 7주간 사육시켰다. 알코올 섭취는 실험시작 3주째부터 ethanol을 급수물에 10% 맞추어 섭취군과 비섭취군으로 나누어서 섭취군에서는 제한없이 먹게 하였다. 얻어진 결과를 요약하면 다음과 같다. 혈액과 소변의 Se의 수준은 식이내 Se 수준의 차이에 따라 유의적인 차이를보여 식이내 Se수준이 높은 H-군, 정상적으로 섭취한 C-군, 결핍된 L-군의 순으로 높았으며 알코올 섭취군들은 낮은 경향을 보였다. 간장, 신장의 Se의 농도는 식이 Se 수준에 비례하여 H-, C-, L- 순으로 높았으며 알코올 섭취로 인한 Se농도의 변화는, 알코올을 섭취한 군둘의 Se 농도가 낮은 경향이나 신장에서는 반대로 높은 경향이었다. 전자현미경을 통한 관찰에서 심근세포 조직형태의 변화는 식이내 Se수준이 높은 H-군들, Se수준이 낮은 L-군에서 lysosome의 증가 및 지방적 출현, mitochondria의 종창, 특히 모세혈관의 내피세포에 심한 종창이 일어났고, 알코올을 섭취한 군들에도 심근세포의 종창, lysoscme의 증가, mitochondria 변화를 보였다. Se을 과잉으로 섭취하고 vitamin E를 정상으로 섭취한군(HC-)에서는 특이한 변화가 없었으나 알코올을 투여한 HCA군에서 지방적이 나타났다. 특히 Se과 vitamin E이 결핍되고 알코올을 섭취한 LLA군에서는 lysosome의 증가 및 지방적이 많이 증가 하였다.

• Journal of Animal Science and Technology
• /
• 제46권4호
• /
• pp.635-644
• /
• 2004

본 시험은 vitamin C와 E 급여가 한우 거세우 쇠고기의 보존에 미치는 영향을 규명하기 위하여 실시하였다. 시험구는 대조구(비타민 C 비급여 및 미타민 E 20IU/kg feed), 비타민 C 급여구(비타민 C 0.1%/kg feed 및 비타민 E 20IU/kg feed), 비타민 E 급여구(비타민 E 200IU/kg feed) 및 비타민 C와 E 혼합 급여구(비타민 C 0.1%/kg feed 및 비타민 E 220 IU/kg feed)의 네 처리구로 나누어 출하전 90일 동안 사양시험을 실시하였고, 도축 후 5$^{\circ}C$에서 정장하면서 1, 3, 5, 7 및 10일째 배최장근의 pH, TBARS, 표면색, 육색소 분포, 보수력, 가열감량, 육즙손실 및 포장감량을 조사하였다. pH는 저장기간이 경과함에 따라 증가하였고, 비타민 C와 E 혼합 급여구가 저장 전 기간동안 높게 나타났다. 지방산패도는 저장기간이 경과함에 따라 증가하였고, 비타민 급여구가 대조구보다 낮았고, 특히 비타민 C와 E를 혼합하여 급여하였을 때 지방산패도가 낮았다. Hue angle은 저장기간이 경과함에 따라 높아졌고, 특히 대조구가 비타민 급여구보다 상당히 증가 하였다. Metmyoglobin 함량은 저장기간이 경과 할수록 증가하였고, 특히 대조구가 비타민 급여구보다 유의적(p<0.05)으로 높았다.Oxymyoglobin 함량은 저장기간이 경과하면 감소하였으나, 비타민 E 또는 C와 E 혼합 급여구가 대조구보다 유의적(P<0.05)으로 높았다. 이는 비타민 C와 E를 급여하면 쇠고기의 육색을 안정화시키는 것으로 사료된다. 보수력은 저장 7일째까지는 처리구간에 유의적인 차이는 없었고, 가열감량은 저장기간별로는 차이가 없었으나, 비타민 E 급여구 또는 비타민 C와 E 혼합 급여구가 대조구 및 비타민 C 급여구보다 저장 전기간 동안 유의적(P<0.05)으로 높았으며, 육즙손실과 저장감량은 저장기간이 경과함에 따라 증가하는 경향을 나타내었다. 그러므로 본 시험결과에 의하면 사료 중에 비타민 C 0.1% 또는 사료 kg당 비타민 E 220 IU를 첨가하면 지방산화가 지연되고, 또한 사료 중에 비타민 C 0.1%와 사료 kg당 비타민 E 220 IU를 함께 첨가해주면 육색이 안정화되는 것으로 사료된다.

• Fisheries and Aquatic Sciences
• /
• 제13권2호
• /
• pp.118-126
• /
• 2010

A feeding trial was conducted to investigate the effects of different levels of dietary n-3 highly unsaturated fatty acids (HUFA) (1.1-5.6%) and vitamin E (70 and 400 mg/kg) on the growth and body composition of juvenile rockfish. Six isonitrogenous (45% crude protein) and isolipidic (17% crude lipid) diets were formulated to contain graded levels of n-3 HUFA and vitamin E. Diets 1, 2 and 3 consist of 400 mg vitamin E/kg diet with graded levels of 1.1, 3.0, and 5.6% n-3 HUFA, respectively. Graded levels of n-3 HUFA (1.1, 3.0, and 4.0%) were added in diets 4, 5 and 6, respectively, containing 70 mg vitamin E/kg diet each. At the end of feeding trial, growth performance of rockfish was affected by neither dietary n-3 HUFA nor vitamin E levels. Feed efficiency and hepatosomatic index were slightly decreased (P<0.05) with increment of dietary n-3 HUFA at each dietary vitamin E level. Dietary vitamin E and n-3 HUFA levels did not affect proximate composition and vitamin E concentration in the dorsal muscle of rockfish. Liver moisture and crude protein contents positively related to dietary n-3 HUFA levels. Liver lipid content and hematocrit value were significantly decreased (P<0.05) by increasing dietary n-3 HUFA levels. Eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) concentrations in the dorsal muscle significantly correlated to dietary n-3 HUFA levels, except for fish fed the diet 6 containing 4% n-3 HUFA and 70 mg vitamin E/kg diet. EPA concentration in the dorsal muscle of fish fed the diet 6 was significantly lower than that of fish fed the diets 2, 3 and 5. The present findings suggest that feeding of diets containing excessive n-3 HUFA level with varying addition of vitamin E may alter fatty acid composition in the dorsal muscle, but do not affect growth of juvenile rockfish.

• Journal of Nutrition and Health
• /
• 제32권3호
• /
• pp.239-247
• /
• 1999

With the increasing elderly population in Korea, dementia has become a mojor health problem in Korea. Several studies have been conducted on the association between dementia and dietary intake, especially vitamin E and vitamin C. The purpose of this study was to investigate the effect of vitamin E and vitamin C supplementation on the decrease in cognitive function induced by scopolamine(300mg/kg) in rats. Rats were divided into three groups : control, vitamin C, and vitamin E supplementation(2.4g Vit C or Vit E /100g diet) and fed the diets for 6 weeks. There were significant decreases in active avoidance response and brain acetylcholinesterase activity in the control group, but no significant differences were observed in the vitamin E and C groups after scopolamine treatment. Brain dopamine concentration of vitamin E and C groups was significantly higher than those of control group after scopolamine injection. The concentrations of brain norepinephrine also showed similar tendence, even though it was not statistically significant. These results indicate that vitamin E and vitamin C may protect against the cognitive function decrease induced by scopolamine. However, it is still unclear how vitamin E and C influence brain neurotransmitters and improve cognitive function. Further study is need to elucidate the role of vitamin E and C supplementation in the prevention of dementia.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권4호
• /
• pp.500-506
• /
• 2006

An experiment was conducted with twenty crossbred male calves (7-15 days old) divided into 4 different experimental groups on the basis of body weights to study the effect of vitamin E supplementation on the serum ${\alpha}$-tocopherol concentration and immune response of the calves. All the calves were fed on milk and calf starter up to 13 weeks and afterwards, they were fed on concentrate mixture and oat hay up to 32 weeks of age. In addition, the calves in groups I, II, III and IV were supplemented with 0, 125, 250 and 500 IU feed grade DL-${\alpha}$-tocopheryl acetate, respectively. Blood samples were collected at 0 day and subsequently at 1, 2, 3, 4, 6 and 8 months of age to monitor the serum ${\alpha}$-tocopherol concentration in crossbred calves. After 24 weeks of experimental feeding, 4 animals from each group were intramuscularly inoculated with single dose (3 ml) of Haemorrhagic septiceaemia (Pasteurella multocida P52 strain) oil adjuvant vaccine. The cumulative group mean serum ${\alpha}$-tocopherol concentration (${\mu}g/100ml$) was 88.12, 210.11, 235.21 and 294.02 in-groups I, II, III and IV, respectively and differed significantly (p<0.001) among the four groups. Lymphocyte stimulation indices (LSI) did not differ among the groups significantly. The pooled mean ELISA antibody titer against Pasteurella multocida (P52 strain) was 788.02, 926.85, 1,214.00 and 1,109.51 for group I, II, III and IV, respectively, which indicated higher antibody titer in groups supplemented with vitamin E as compared to the control group. It may be concluded that vitamin E supplementation increased the ${\alpha}$-tocopherol concentration in serum and dietary supplementation of vitamin E at higher level has a humoral immune enhancing effect against killed bacterial antigen.

• 한국가축번식학회지
• /
• 제24권2호
• /
• pp.163-170
• /
• 2000

본 연구는 rBST, vitamin E 및 selenium (Se)의 투여가 한우 종모우의 혈액성분 및 호르몬의 농도변화에 미치는 영향을 검토하였다. 1. rBST 투여구, Vit. E 투여구 및 Se 투여가 한우 종모우의 혈액성분에 미치는 효과를 조사한 결과, 혈중 albumin의 함량은 Se 투여구가 대조구, rBST 투여구 및 Vit. E 투여구보다 통계적으로 유의하게 높은 함량을 나타났으며 (P＜0.05), 혼합투여구는 여타구 (대조구, rBST 투여구 및 vitamin E 투여구)보다 통계적으로 유의하게 높게 나타났다 (P＜0.05). 혈중 BUN (Blood Urea Nitrogen)과 creatinine의 함량은 rBST 투여구가 여타구보다 통계적으로 유의하게 높게 나타났다 (P＜0.05). 혈중 total protein 함량은 rBST 투여구, Se 투여구 및 혼합투여구가 대조구보다 통계적으로 유의하게 높은 함량을 나타냈다 (P＜0.05). 혈중 calcium, cholesterol, glucose, inorganic phosphoros 의 함량은 투여구간에 차이가 없었다 (P＞0.05). 2. rBST, Vit. E 및 Se 투여가 한우 종모우의 혈중내 호르몬의 변화에 미치는 효과를 조사한 결과, 혈중 estradiol의 농도는 Se 투여구와 혼합투여구가 여타구 (대조구, rBST 투여구 및 Vit. E 투여구 )보다 다소 높게 나타났지만 유의차는 인정되지 않았으며 (P＞0.05), 혈중 testeserone의 농도는 투여구간에 커다란 차이는 없었다 (P＞0.05).

• 한국가금학회지
• /
• 제21권4호
• /
• pp.227-237
• /
• 1994

This study was conducted to investigate the interaction of vitamins A and E on their transfer from diet to chicken eggs and the effect of vitamin A flood-dosing on its concentration in eggs and livers. In Experiment I, forty-two 45-wk-old brown layers (Bobeock) were divided into seven groups and fed one of seven diets: control, three vitamin A supplemented diets(8, OOO, 16, 000, and 64, 000 IU /kg diet) or three vitamin E supplemented diets (50, 100, and 200 IU/kg diet). In Experiment II, a total of thirty-two 35-wk-old white layers (Hy-ine) were divided into four groups and fed one of four diets :control, vitamin A 20, 00O+vitamin E 200 TU /kg, vitamin A 50, O00+vitamin E 200 IU /kg or vitamin A 100, OO0+vitamin E 200 lU/kg supplemented diets. In Experiment III, a total of fifty-six 35-wk-old white layers (Hy-line) was divided into four groups and fed one of four diets: control or three vitamin A supplemented diets (80, 000, 120, 000 and 160, 000 lU/kg diet). In Experiment I, vitamin E levels of egg yolk in hens fed the vitamin A supplemented diets decreased as dietary vitamin A level increased (P

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권11호
• /
• pp.1614-1618
• /
• 2003

Twenty crossbred (HF${\times}$Tharparkar) dry pregnant cows were divided into four equal groups. They were supplemented with 1,000 I.U. $\alpha$-tocopheryl acetate from 0 (group I), 15 (group II), 30 (group III) and 60 (group IV) days before parturition to 1month of lactation. All the cows were kept under similar feeding and management conditions. Blood plasma samples collected on specific days were analyzed for $\alpha$-tocopherol, retinol, total antioxidant activity (FRAP), immunoglobulin and calcium. Plasma $\alpha$-tocopherol concentration at 30 days prepartum averaged 3.5, 4.1, 4.4 and $3.9{\mu}g/ml$ and decreased by 50.0, 41.4, 34.1 and 33.3 percent on the day of parturition in the four respective groups. After calving, plasma vitamin E started to recover earlier in groups II, III and IV as compared to group I. Mean plasma $\alpha$-tocopherol concentration at 21 days postpartum was significantly higher in groups II, III and IV (2.9, 3.5 and $3.1{\mu}g/ml$) compared to group I ($1.9{\mu}g/ml$) cows. Plasma retinol concentration also showed a substantial decrease in all the groups on the day of calving but recovered to its normal value at 3 weeks postpartum. Plasma total antioxidant activity averaged 901, 895, 859 and $875{\mu}mol/l$ in the four respective groups on 30 days prepartum and decreased on the day of calving in all the groups, but the decrease was less in groups III and IV. Plasma immunoglobulin concentration was higher in group IV, followed by groups III, II and I, respectively, showing better immune status of vitamin E supplemented cows due to less oxidative stress. Supplementation of vitamin E resulted in higher plasma calcium concentration. The data showed that vitamin E supplementation should be started at least 30 days prepartum to reduce oxidative stress in periparturient cows.