• Korean Journal of Veterinary Service
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• v.45 no.1
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• pp.19-30
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• 2022

In this study, a simple loop-mediated isothermal amplification (LAMP) combined with visual detection method (vLAMP) assay was developed for the rapid and specific detection of African swine fever virus (ASFV), overcoming the shortcomings of previously described LAMP assays that require additional detection steps or pose a cross-contamination risk. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubation for 40 min at 62℃. The assay specifically amplified ASFV DNA and no other viral nucleic acids. The limit of detection of the assay was <50 DNA copies/reaction, which was ten times more sensitive than conventional polymerase chain reaction (cPCR) and comparable to real-time PCR (qPCR). For clinical evaluation, the ASFV detection rate of vLAMP was higher than cPCR and comparable to OIE-recommended qPCR, showing 100% concordance, with a κ value (95% confidence interval) of 1 (1.00~1.00). Considering the advantages of high sensitivity and specificity, no possibility for cross-contamination, and being able to be used as low-cost equipment, the developed vLAMP assay will be a valuable tool for detecting ASFV from clinical samples, even in resource-limited laboratories.

• Journal of Microbiology and Biotechnology
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• v.33 no.8
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• pp.1091-1100
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• 2023

Human papillomavirus (HPV) types 16 and 18 are the major causes of cervical lesions and are associated with 71% of cervical cancer cases globally. However, public health infrastructures to support cervical cancer screening may be unavailable to women in low-resource areas. Therefore, sensitive, convenient, and cost-efficient diagnostic methods are required for the detection of HPV16/18. Here, we designed two novel methods, real-time ERA and ERA-LFD, based on enzymatic recombinase amplification (ERA) for quick point-of-care identification of the HPV E6/E7 genes. The entire detection process could be completed within 25 min at a constant low temperature (35-43℃), and the results of the combined methods could be present as the amplification curves or the bands presented on dipsticks and directly interpreted with the naked eye. The ERA assays evaluated using standard plasmids carrying the E6/E7 genes and clinical samples exhibited excellent specificity, as no cross-reaction with other common HPV types was observed. The detection limits of our ERA assays were 10⁰ and 10¹ copies/µl for HPV16 and 18 respectively, which were comparable to those of the real-time PCR assay. Assessment of the clinical performance of the ERA assays using 114 cervical tissue samples demonstrated that they are highly consistent with real-time PCR, the gold standard for HPV detection. This study demonstrated that ERA-based assays possess excellent sensitivity, specificity, and repeatability for HPV16 and HPV18 detection with great potential to become robust diagnostic tools in local hospitals and field studies.

• Journal of Korean Society of Rural Planning
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• v.14 no.1
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• pp.59-71
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• 2008

The landscape commonly refers to the appearance of the land, including its shape, texture, and colours. Among many factors contributing to the landscape, tree and plants factors playa significant role in rural landscape as a colours. Therefore the rural traditional theme village where had been designated under the rural traditional theme village since 2007 in Naegok-ri, Eohang-myeon, Haman-gun has planned to design the tree plant planning for creating a unique rural landscape. The landscape proposal for the new Rural Village in Naegokri, Yeohang-myeon, Haman-gun suggests three main strategies; 1) The riverside area and reservoir area adjoined forest was required to be prepared to offer organization waterside landscape and forest landscape through landscape planting and selecting trees i.e. able to reflect sense of the season that promote high quality values of landscape area in rural. 2) The area adjoined stable was required to strengthen the screen planting and buffer planting with multiple plant layer structure and trees of branches and leaves closeness, shrubs of beauty flowers in order to improve disamenity landscape and odours. 3) The rural traditional theme village adjoined entrance was required to open space such as multipurpose garden, specialized or themed garden which include Landmark factors and prepared to the space with the various theme and visual diversity in order to take charge of function as landmark characteristic in Naegok-ri, Eohang-myeon, Haman-gun, and so on. In conclusion, this winning principal purpose of this study is applied to basic tree plant model for sustainable rural landscape creation in rural areas by selecting beautiful landscape plants and the tree plant planning.

• Proceedings of the KSR Conference
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• 2009.05a
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• pp.860-865
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• 2009

This thesis deals with application of Windows CE for Embedded System and the development methode of "Speed Gate Controller Tester" taking advantage of development program. We can improve reliability, stability and convenience of maintenance work as use of "Speed Gate Controller Tester" which was developed and applied "Embedded System" We can provide customers with more qualified service naturally because of the higher rate of operation which makes people use more pleasant and comfortable subway facility. And also it is possible to manage processor, time schedule and hardware resource as application of Embedded System and Windows CE. Embedded System applied OS Windows CE makes it possible to develop other various products, another application of equipment and tester. Thus this paper treats problems the moment developed and the present condition, development process, field application results.

• Korean Journal of Environmental Agriculture
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• v.35 no.2
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• pp.152-157
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• 2016

BACKGROUND: Since bottom ash (BA) contains considerable amounts of CaO and MgO, it could be a useful amendment to increase soil pH and to immobilize cadmium (Cd). This study was conducted to evaluate effect of BA application in reducing Cd phytoavailability.METHODS AND RESULTS: Bottom ash was applied at the rate of 0, 20, 40, and 80 Mg/ha to Cd contaminated soil, and then lettuce was cultivated under field condition. soil pH and net negative charge increased slightly with increasing BA application; however, there was no statistical difference among the rates. Water soluble, exchangeable+acidic, reducible, and oxidizable fraction of Cd decreased with increasing bottom ash application rate, whereas residual fraction of Cd increased with increasing bottom ash application rate. Lettuce yield increased with rate of bottom ash up to 40 kg/ha. Visual evidences of cadmium toxicity and growth inhibition were not found during lettuce cultivation.CONCLUSION: Bottom ash was effective to reduce phytoextractability of Cd and to increase lettuce yield. Conclusively, BA could be a good soil amendment to reduce Cd phytoavailability in contaminated arable soil.

• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.3 s.116
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• pp.104-119
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• 2006

The eastern rear business complex of the Gwangyang Harbor (second stage) has undergone the 'alternative bid' process by which a construction company is selected based on their suggestions for an alternative plan and the bid for the construction expenses is based on the previously completed original plan, thereby implicitly accepting the arrangement and scale of the park which was presented in the original plan. It is possible, however, that the concept and plan of each park needs to be approached in a new way within the extent of given design guidelines. This study focuses on the alternative plan process and the Symbol Park (the second neighborhood park) and Waterfront Park (the third neighborhood park) among the five neighborhood parks in the eastern rear business complex (second stage). These two parks provide scale and function for the structure of the park and greenways system at the eastern rear complex. The neighboring waterway is a significant resource for emphasizing the pre-development features of the site. The alternative plan process focuses on determining the resources for the park's plan based on the environmental analyses of the site and utilizing the results. That is, through an analysis of the current state, all available resources are determined and the facilities and activities are derived based on these results. By this, Symbol Park and Waterfront Park reflect the regional features of the eastern rear complex, and these parks will feasibly perform the role of the region's key parte. The limit of this study is the fact that there is no chance for landscape architecture to actively play a role in the creation of the master plan, land utilization plan, and plan for the complex. Therefore there is a problem that the function scale and location of the park are not distributed appropriately in the rear complex. Also the plan for protecting the waterway's shore, a significant resource, only considers the structural stability of the engineering works, degrading the visual and ecological quality. These experiences will be used as a case for further designing the industrial complex and for the resulting park plan.

• Korean journal of applied entomology
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• v.58 no.2
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• pp.143-149
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• 2019

This study was conducted to examine the attractiveness of yellow sticky trap (YST) for insect pests by the angle of inclination of the trap surface. In strawberry farms with high bed system, YSTs were installed to attract insect pests in vertical direction with one surface, horizontal direction with upper and under surface, and angle of $45^{\circ}$ with upper and lower sticky surface. Thrips (Frankliniella occidentalis in dominance) and Sciaridae (Bradysia agrestis in dominance) species were more attracted on upper surface of $45^{\circ}$ angle trap, vertical surface and upper surface of horizontal trap than on lower surface of $45^{\circ}$ angle trap or horizontal trap. Cicadellidae (Empoasca vitis in dominance) species were caught more on vertical surface and upper surface of $45^{\circ}$ angle trap than on other traps. There were no specific trend in capture of aphid (Aphis gossypii in dominance) and white fly (Trialeurodes packardi in dominance) species among traps, probably because of a low density of the pest species.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1098-1101
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• 2004

The ordinary stereo image of an object consists of data of left and right views. Therefore, the left and right image pairs have to be transmitted simultaneously in order to display 3-dimentional video at the remote site. However, due to the twice data in comparing with a monoscopic image of the same object, it needs to be compressed for fast transmission and resource saving. Hence, it needs an effective coding algorithm for compressing stereo image. It was found previously that compressing left and right frames independently will achieve the compression ratio lower than compressing by utilizing the spatial redundancy between both frames. Therefore, in this paper, we study the stereo image compression technique based on the multiresolution wavelet transform using varied disparity-block size for estimation and compensation. The size of disparity-block in the stereo pair subbands are scaling on a coarse-to-fine wavelet coefficients strategy. Finally, the reference left image and residual right image after disparity estimation and compensation are coded by using SPIHT coding. The considered method demonstrates good performance in both PSNR measures and visual quality for stereo image.

• Proceedings of the KSRS Conference
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• 2002.10a
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• pp.183-190
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• 2002

In this paper forest fire statistical information management system is constructed on web environment using web based GIS(Geographic Information System) technology. Though this system, general users can easily access forest fire statistical information and obtain them in visual method such as maps, graphs, and text if they have web browsers. Moreover, officials related to forest fire can easily control and manage all information in domestic by accessing input interface, retrieval interface, and out interface. In order to implement this system, IIS 5.0 of Microsoft is used as web server and Oracle 8i and ASP(Active Server Page) are used for database construction and dynamic web page operation, respectively. Also, Arc IMS of ESRI is used to serve map data using Java and HTML as system development language. Through this system, general users can obtain the whole information related to forest fire visually in real time also recognize forest fire prevention. In addition, Forest officials can manage the domestic forest resource and control forest fire dangerous area efficiently and scientifically by analyzing and retrieving huge forest data through this system. So, they can save their manpower, time and cost to collect and manage data.

• Journal of Soil and Groundwater Environment
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• v.18 no.3
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• pp.11-22
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• 2013

SEAWAT, a linked modeling program of Visual MODFLOW was used to analyze the change in groundwater levels and salinity related groundwater dam construction in Cheongsan island, Wando-Gun, Jeollanam-Do. The steady-state model results show the groundwater flow and salinity distribution of the studied area. The groundwater flows from north-west and south-east highlands into the river, located in the middle part of the basin, and is eventually discharged to the ocean. Part of the sea water infiltrates into the river; and through the estuary's alluvium aquifer, the sea water intrusion takes place spreading to about 830 m from the ocean. The transient model results show that after the groundwater dam construction, groundwater levels will rise to a maximum of 2.0 m upstream, and the groundwater storage will increase 21,000 after 10 years. Meanwhile 31% of the total area affected by sea water intrusion will decrease. To conclude, the groundwater dam is a very useful method for a secure water resource in preparation for drought and water shortages in the island regions.