• 식물병연구
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• 제13권1호
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• pp.30-33
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• 2007

석량풋콩 품종의 콩밭에서 모자이크 증상이 약하게 나타난 186개의 잎을 채집하여, Cowpea mosaic virus(CPMV)에 대한 ELISA 검정을 하였다. 그 결과 93개의 시료가 양성반응을 보였으며, 이 가운데 55개의 시료는 매우 높은 바이러스 밀도를 나타내고 있었다. 이러한 결과는 석량풋콩 품종의 동부모자이크병은 ELISA 검정, 바이러스 순화, 전자현미경검경 결과와 바이러스 표피 단백질분석 결과 Cowpea mosaic virus에 의한 병해로 확인되었다.

• 한국동물위생학회지
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• 제23권3호
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• pp.301-306
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• 2000

In an outbreak of acute porcine diarrhea in newborn piglets, an etiological study was carried out using piglets submitted in Cheju Province Institute for Livestock Promotion(Cheju Veterinary Service for the disease diagnosis). Sixteen piglets(2-7 days old) were collected from 4 farms during outbreaks of diarrhea disease(from January to April 2000). Specimens were taken after necropsy and examined by immunohistochemistry using of monoclonal antibodies for porcine epidemic diarrhea(PED) virus, transmissible gastroenteritis(TGE) virus, and porcine rotavirus. Immunohistochemistry showed that PED virus antigens, but both TGE virus and rota virus antigens not, were localized in the some epithelial cells of the intestines of 14 animals among 16 piglets examined. PEB virus antigens were mainly detected in the cytoplasm of enterocytes. Infected cells, which were most abundant in the villous epithelial cells of the jejunum and ileum, were uncommon in the crypt, epithelial cells, the lamina propria and Peyer's patches of piglets examined. The results suggest that PED virus is one of the most prevailing agents in an outbreak of fatal diarrhea in newborn piglets on Cheju island and PED virus was need to further study to prevent this disease.

• Asian-Australasian Journal of Animal Sciences
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• 제9권4호
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• pp.465-469
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• 1996

Pathological and virological investigations were conducted on suspected outbreaks of infectious bursal disease (IBD) in a broiler farm and five pullet-raising poultry farms of Mymensingh and Tangail districts of Bangladesh. About 80 to 100 percent chicks were affected at the age of 26 to 45 days and mortality varied from 20 to 30 percent in broilers and 40 to 80 percent in layer chicks. Signs, symptoms, gross and microscopic lesions were typical of acute IBD. Several isolates of virus could be obtained by embryo inoculation and the virus was diagnosed as infectious bursal disease virus (IBDV) by agar gel immunodiffusion test (AGID). The virus isolate belonged to the very virulent pathotype of IBDV causing 100 percent mortality in three weeks old chicks on experimental infection.

• 한국유기농업학회지
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• 제23권1호
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• pp.123-131
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• 2015

2006년부터 2010년까지 국내 고추 노지재배 포장을 대상으로 경상남도와 제주도를 제외한 강원도 11개 지역 등 전국에 걸쳐 총 83개 지역을 중심으로 바이러스 이병체를 채집하여 연차별 고추 발생 바이러스의 발병률, 바이러스 종류 및 감염 형태를 조사하였다. 2006년부터 2010년까지의 고추에서의 평균 단독 감염률은 46.6%, 복합 감염률은 48.0%로 조사되어 고추 생산에 있어서 가장 큰 위해요소는 바이러스 발병으로 나타났다. 바이러스별로는 CMV가 평균 34.8% 발병률로 가장 우점 바이러스로 조사되었다. 반면 BBWV2는 낮은 발병율을 보여주었으며, TSWV는 충남과 전남지역에 국한되는 것으로 조사되었다. 따라서 고추 노지재배 시 발생하는 바이러스의 위해도를 발병률에 따라 구분하면 CMV, PMMoV, 그리고 PepMov 순으로 조사되었다. 또한 복합 감염의 발생도 모든 조사지역 시료에서 검출되었으며, 이에 대한 감염 별 바이러스 종류와 발생률을 분석하였다. 복합 감염에서 가장 빈번하게 검출된 바이러스는 CMV로, CMV+BBWV2, CMV+PepMoV, CMV+PMMoV의 복합 감염형태가 검출률이 높게 나타났다. 따라서 고추의 유기농 재배 생산에 가장 큰 위협요인으로 작용하는 바이러스의 적절한 대책마련을 위한 지속적인 바이러스 모니터링과 3종 이상의 복합 감염 위해성을 판단하기 위한 추가 연구가 필요하다고 사료된다.

• 한국가금학회지
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• 제39권2호
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• pp.113-120
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• 2012

본 연구를 통하여 Vero 세포에서 강독형 NDV인 Kr005주를 55대 연속 계대하여 Vero 세포에 적응된 Kr005/V 주를 작성하였다. Kr005/V 주는 Vero 세포에서 $10^{7.8}/mL$의 높은 바이러스 증식성을 보였으며, 감염세포는 cell rounding과 같은 CPE를 형성하였다. 또한 이열성(heat labile) NDV Kr005주와 달리 NDV Kr005/V 주는 내열성(thermostable)을 보였다. 계태아에서 평균 치사 시간(MDT)를 측정한 결과, 강독형인 Kr005주(49.6시간의 MDT)와 달리 Kr005/V 주는 120시간이상의 MDT를 보여 약독형 바이러스(lentogenic virus)로 분류되었다. Kr005/V 주의 HN 단백질과 F 단백질의 염기서열을 분석한 결과, HN 단백질의 443번째 아미노산에서만 변이(T에서 S)가 관찰되었다. Kr005/V 주와 Vero 세포를 사용하여 야외 닭 혈청을 대상으로 혈청중화 항체가를 측정한 결과, Kr005주와 CEF 세포를 사용하여 측정한 혈청중화 항체가와 97%의 상관성을 나타내었다.

• 한국응용곤충학회지
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• 제16권2호
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• pp.87-92
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• 1977

1963년부터 중부지방에 큰 피해를 주고 있는 맥류의 바이러스병을 동정하기 위하여 전자현미경에 의한 바이러스입자 관찰과 매개충을 이용한 전염시험 및 기주범위조사를 한 결과 정상입자의 평균 크기는 길이가 300-370nm였고 폭이 57-60nm였다. 매개충은 애멸구 Laodelphax striatellus(Fallen)로서 충체내 잠복기간은 7-19일이였고 대부분 10일간이었다. Host range는 보리, 옥수수, 밀, Rye맥, 구리 등으로 옥수수외의 기주식물들은 포장에서도 발병이 되고 있다. 그러므로 중부지방에서 발생되고 있는 맥류 바이러스병은 대부분이 북지모자익바이러스병으로 매년 $10\%$내외의 감염율을 보여주고 있으며 춘파맥에서는$100\%$ 감염 될 때도 있다.

• Journal of Animal Science and Technology
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• 제57권4호
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• pp.10.1-10.7
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• 2015

Control and prevention of foot and mouth disease (FMD) by vaccination remains unsatisfactory in endemic countries. Indeed, consistent and new FMD epidemics in previously disease-free countries have precipitated the need for a worldwide control strategy. Outbreaks in vaccinated animals require that a new and safe vaccine be developed against foot and mouth virus (FMDV). FMDV can be eradicated worldwide based on previous scientific information about its spread using existing and modern control strategies.

• The Plant Pathology Journal
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• 제23권3호
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• pp.215-218
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• 2007

The occurrence and relative incidence of viruses including Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Papaya rings pot virus (PRSV), and Watermelon mosaic virus (WMV), Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Melon necrotic spot virus (MNSV) were surveyed from main melon (Cucumis melo L.) production areas in Jeonnam province during 2000-2002. Virus disease incidences of melon cultivating fields were 0% and 11% in spring and fall 2000; 40%, 2.1%, and 8.8% in spring, summer, and fall 2001; and 6.3 % in spring 2002 in main cultivated areas in Jeonnam province, respectively. Field disease incidences of melon virus infections were 0% and 18.8% in spring and fall 2000; 50%, 38.5%, and 82.6% in spring, summer, and fall 2001; and 47.4% in spring 2002, respectively. Total of 101 melon samples showing typical disease symptoms were collected from 2000 to 2002 and tested for virus infection by RT-PCR. Potyvirus-specific DNA fragments for WMV, ZYMV, and PRSV were amplified from 46, 5, and 4 samples, respectively. MNSV specific DNA fragment was amplified from 18 samples. CMV-specific DNA fragment was detected from only 3 samples.

• 대한수의학회지
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• 제62권3호
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• pp.24.1-24.7
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• 2022

Incidences of major feline viral diseases provide basic information for preventing viral disease in cats. Despite the growing interest in feline viral diseases, sero-surveillances have been lacking. In this study, we analyzed the diagnoses of feline viral diseases and conducted a sero surveillance of feline panleukopenia virus (FPV), feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), and feline infectious peritonitis virus (FIPV) in Korean cats. Of the 204 confirmed cases since 2015, the numbers of diagnoses for FPV, FIPV, FCV, feline influenza virus, and FHV-1 were 156, 32, 12, 3, and 1 case, respectively. In total, 200 sera, collected between 2019 and 2021, were screened for the presence of antibodies against FPV, 2 FCVs, FHV-1, and FIPV using a hemagglutination inhibition test and a virus-neutralizing assay (VNA). The overall seropositive rates in cats tested for FPV, the 2 FCVs, FHV-1, and FIPV were 92.5%. 42.0%, 37.0%, 52.0%, and 14.0%, respectively. A low correlation (r = 0.466) was detected between the VNA titers of 2 FCV strains. The highest incidence and seropositive rate of FPV reveal that FPV is circulating in Korean cats. The low r-value between 2 FCVs suggests that a new feline vaccine containing the 2 kinds of FCVs is required.

• 식물병연구
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• 제15권1호
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• pp.8-12
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• 2009

Virus disease surveys of strawberries cultivated and preserved as germplasm resources in Korea was conducted during 2007-2008. Virus detection was conducted by RT-PCR using total RNAs extracted from strawberry samples. We detected the infection with Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry vein banding virus (SVBV) and Strawberry pallidosis associated virus (SPaV) while no infection with Strawberry crinkle virus (SCV), Strawberry necrotic shock virus (SNSV), Strawberry latent ring spot virus (SLRSV) and Arabis mosaic virus (ArMV) was observed. The infection rate of virus disease on 4 cultivars including Seolhyang, Maehyang, Gumhyang, and Dahong, bred in Korea, was 0.1, 1.9, 0, and 0%, respectively. Surprisingly, however, cultivar Red Peal introduced from Japan in 1997 revealed 48.3% virus infection rate. SMYEV, SMoV and SPaV were also identified in strawberries growing in the farm fields of Korea. In the field, however, SMYEV was the most predominant virus (97.4%) among those 3 identified viruses. SVBV was detected only in strawberry kept as a germplasm.