• 대한수의학회지
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• 제40권1호
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• pp.42-48
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• 2000

Akabane disease is transmitted through mosquitoes in cattle, sheep and goats. It shows congenital abnormalities including encephalomyetitis, hydranencephaly, neurogenic arthrogryposis, and deformed neonatal calves. Akabane viruses, 93FMX and K-9 strain, were isolated from fetal matrix of aborted cow and blood of healthy cow, respectively. S gene sequences of 93FMX and K-9 showed 100% homology with that of OBE-1 strain isolated in Japan. Based upon our sequencing data, we synthesized specific primers for PCR diagnosis. Using these primers, we were able to amplify the S gene of Akabane virus not only from the culture fluid of Vero cells but also from the brain tissue of suckling mouse inoculated with, Akabane virus. These PCR products were confirmed by Southern blot hybridization. Not only the sensitivity of PCR test was high enough to detect the viruses of $10^{1.0}TCID_{50}/ml$, but also the time for diagnosis was significantly shorter than that of the virus isolation by tissue culture method. This method was also effective for the detection of Akabane virus in the cerebrum of fetus. RT-PCR method may be used for a useful diagnostic test of the clinical cases of Akabane disease.

• 융합보안논문지
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• 제5권1호
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• pp.53-62
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• 2005

virus 방역 체계 관리는 network infra 구조관리, traffic 소통경로관리, 방역 zone 설정, gateway구간 방역관리이다. 본 논문은 일반적인 방역체계 구조의 성격과 취약점을 진단하고 이를 개선할 수 있는 대책으로서 개선된 방역체계를 설계하였다. 또한 설계된 방역체계와 configuration, mechanism하에서는 어느 정도의 개선효과 나타나고 있는지 분석하였다. 개선된 다단계 방역체계 하에서는 gateway 단계에서 불필요한 mail을 걸러줌으로서 server에 주는 부하는 감소하며 virus wall상의 CPU 부하의 감소와 virus 치료율의 상승으로 송신 적체 건수는 감소하고 시스템 process수는 증대하고 있다.

• 한국사이버테러정보전학회:학술대회논문집
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• 한국사이버테러정보전학회 2004년도 제1회 춘계학술발표대회
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• pp.187-198
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• 2004

virus방역 체계 관리는 network infra구조관리, traffic소통경로관리. 방역 zone설정, gateway구간 방역관리이다. 본 논문은 일반적인 방역체계 구조의 성격과 취약점을 진단하고 이를 개선할 수 있는 대책으로서 개선된 방역체계를 설계하였다. 또한 설계된 방역체계와 configuration, mechanism하에서는 어느 정도의 개선효과 나타나고 있는지 분석하였다. 개선된 다단계 방역체계 하에서는 gateway 단계에서 불필요한 mail을 걸러주므로서 server에 주는 부하는 감소하며 virus wall상의 CPU부하의 감소와 virus 치료율의 상승으로 송신 적체 건수는 감소하고 시스템 process수는 증대하고있다.

• Fisheries and Aquatic Sciences
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• 제1권2호
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• pp.187-191
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• 1998

Chemotherapy can not be applied for the control of fish viral diseases because viruses depend on host machinery for their replication. Although new control strategies including vaccination are under development, avoidance of virus introduction by rapid and correct diagnosis is the best way of fish viral disease control. Although observation of virus particles with an electron microscope is an easy method for virus detection, it take a few days for the sample preparation. In order to shorten the sample preparation time, microwave radiation was applied in the procedure. With this method, 15 seconds was enough for fixation of virus infected fish samples or cultured cells inoculated with infectious hematopoietic necrosis virus, which takes 2-4 hours with routine methods. Also four minutes was enough for polymerization of embedding resin which takes 24-48 hours with routine methods. Samples prepared with microwave were good enough for direct electron microscopic observation and immunogold labeling assay.

• The Plant Pathology Journal
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• 제39권3호
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• pp.303-308
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• 2023

The global climate change and international trade have facilitated the movement of plants across borders, increasing the risk of introducing novel plant viruses in new territories. Ixora coccinea exhibited virus-like foliar symptoms, including mosaic and mild mottle. An Oxford Nanopore Technologies-based compact and portable MinION platform was used to identify the causal viral pathogen. The complete genome sequence of jasmine virus H (JaVH; 3867 nt, JaVH-CNU) was determined and found to share 88.4-90.3% nucleotide identity with that of Jasminum sambac JaVH isolate in China. Phylogenetic analysis based on the complete amino acid sequences of RNA-dependent RNA polymerase and coat protein revealed that JaVH-CNU was grouped separately with other JaVH isolates. This is the first report of a natural JaVH infection of I. coccinea. The application of rapid nanopore sequencing for plant virus identification was demonstrated and is expected to provide accurate and rapid diagnosis for virus surveillance.

• 식물병연구
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• 제16권1호
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• pp.1-9
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• 2010

Among the plant specimens requested from agricultural actual places of farmers, Agency of agricultural extension services and so forth for the diagnosis of plant virus diseases in 2009, the rate of crop types was 87.5% for vegetables, 4.0% for upland crops and 3.5% for orchids. In vegetables, the crops damaged severely by viral diseases were red pepper and tomato by the infection rate of 51.6% and 26.5%, orderly. Virus species occurring vegetables were 19 and the economically important viruses were Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), Tomato yellow leaf curl virus (TYLCV), Pepper mild mottle virus (PMMoV) with the infection rate of 33.2%, 16.9%, 16.1% and 7.4%, respectively. Rice stripe virus (RSV) occurred at the whole areas of west coast in Korea in 2009, and its incidence was 14.2% mainly on the susceptible cultivars and yield loss was estimated up to 50%. TYLCV was spread at 34 areas of Si and/or Gun, 22 areas in 2009 and 12 in 2008. Distribution of TSWV was expanded newly in 6 areas of Si and/or Gun including Gangryung, Gangwondo in 2009, and its occurrence areas were 23 Si and/or Gun after first incidence at Anyang area in 2004. Tomato bushy stunt virus (TBSV) was incited newly at Gimcheon area in 2009 with the infection rate of 65.2%, and its soil transmission rate was 55.0% in average.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제16권4호
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• pp.225-232
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• 2013

Children with abnormal liver function can often be seen in outpatient clinics or inpatients wards. Most of them have respiratory disease, or gastroenteritis by virus infection, accompanying fever. Occasionally, hepatitis by the viruses causing systemic infection may occur, and screening tests are required. In patients with jaundice, the tests for differential diagnosis and appropriate treatment are important. In the case of a child with hepatitis B virus infection vertically from a hepatitis B surface antigen positive mother, the importance of the recognition of immune clearance can't be overstressed, for the decision of time to begin treatment. Early diagnosis changes the fate of a child with Wilson disease. So, screening test for the disease should not be omitted. Non-alcoholic fatty liver disease, which is mainly discovered in obese children, is a new strong candidate triggering abnormal liver function. Muscular dystrophy is a representative disease mimicking liver dysfunction. Although muscular dystrophy is a progressive disorder, and early diagnosis can't change the fate of patients, it will be better to avoid parent's blame for delayed diagnosis.

• 대한치과의사협회지
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• 제48권5호
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• pp.365-370
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• 2010

Herpes virus family is highly infectious to patients, their families and dentists. The diagnosis of herpes infection is based on the characteristic clinical appearance and the location of the lesions. Herpes Simplex Virus(HSV) usually acquired through direct contact with infected lesions or body fluids, and the prevalence of HSV infection increases progressively from childhood. Primary infections provoke herpetic gingivostomatis typically affects the tongue, lips, gingival, buccal mucosa and palate. Recurrent infections give rise to vesiculo-ulcerative lesions at vermilion border of lip(herpes labialis). In the form of chickenpox, Varicella Zoster Virus(VZV) usually is infected in childhood. VZV spreads in the affected primary afferent nerve to the skin and produces a vesicular rash and pain. Epstein-Barr Virus(EBV) infects B cells and cause infectious mononucleosis. Latent EBV infection has also been implicated in Burkitt lymphoma, nasopharyngeal carcinoma. Cytomegalovirus(CMV) is associated with immune-compromised patient such as organ transplantation and AIDS patients.

• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.313-324
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• 2020

Coronavirus disease 2019 (COVID-19), which causes serious respiratory illness such as pneumonia and lung failure, was first reported in Wuhan, the capital of Hubei, China. The etiological agent of COVID-19 has been confirmed as a novel coronavirus, now known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is most likely originated from zoonotic coronaviruses, like SARS-CoV, which emerged in 2002. Within a few months of the first report, SARS-CoV-2 had spread across China and worldwide, reaching a pandemic level. As COVID-19 has triggered enormous human casualties and serious economic loss posing global threat, an understanding of the ongoing situation and the development of strategies to contain the virus's spread are urgently needed. Currently, various diagnostic kits to test for COVID-19 are available and several repurposing therapeutics for COVID-19 have shown to be clinically effective. In addition, global institutions and companies have begun to develop vaccines for the prevention of COVID-19. Here, we review the current status of epidemiology, diagnosis, treatment, and vaccine development for COVID-19.

• 미생물학회지
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• 제43권1호
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• pp.1-6
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• 2007

Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV), parvovirus B19 (B19)등 4종의 바이러스는 인체에 감염을 일으키는 병원체로서 DNA를 유전물질로 함유한다. 각 바이러스 유전자의 염기서열을 분석하여 EBV CMV, HBV의 pol 유전자와 B19의 ns 유전자에 특이적으로 결합할 수 있는 primer를 설계 제작하고 단일 시험으로 4종의 바이러스를 동시에 검출할 수 있는 다중 중합효소 연쇄반응(Multiplex PCR)법을 확립하였다. Primer 염기서열, PCR 반응조성물의 농도, PCR 반응시간 및 온도조건을 최적화하여 민감도를 증대시킴으로써, 단일 시험으로 5-10 분자수의 유전물질까지 검출이 가능하였다. 또한 4종의 바이러스 사이에 교차반응이 일어나지 않았으며 생체시료를 이용한 시험에서도 특이성과 민감도가 유지됨을 확인하였다. 그러므로 본 연구에서 확립한 다중 중합효소 연쇄반응은 세포배양액 또는 생체 시료에 감염된 4종 DNA 바이러스진단에 효율적으로 이용할 수 있을 것이라고 판단된다.