• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.1
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• pp.68-73
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• 1986

In order to identify the physiological maturity and to determine the proper time of harvesting as fresh seed for cooking with rice and germination ability according to the harvesting time in cowpea (Vigna unguiculata (L.) Walp), this experiment was conducted. The results obtained are as follows; 1. The length and width of pods became maximum size on 10 days after flowering(DAF) and thickness of them increased to 14 DAF and then decreased to 18 DAF. 2. The weight of pod wall reached the maximum on 14 DAF, the thickness of them increased to 8 DAF and decreased to 18 DAF and it was stabilized. 3. The length and thickness of the seeds became larger to 10 DAF, were not changed from 10 DAF to 16 DAF and then decreased after 16 DAF. 4. The seeds coloured 2 days earlier than the pod wall on 8 DAF and the grade increased by degrees to 16 DAF. 5. The moisture content of seeds and pod wall started to decrease on 8 DAF and the moisture content of pod wall was higher than that of seeds in the middle stage but the condition changed after 18 DAF. 6. When the weight of seeds reached the maximum on 16 DAF, the moisture content of them was 54.5%. 7. The content of total nitrogen, phosphate and oil decreased but the carbohydrate increased by degrees along the passing of DAF. The silicic acid did not exist and K$_2$O, CaO, MgO, protein etc. existed but did not show regular tendency. 8. The physiological maturity was 16 DAF and the proper time of harvesting as fresh seed for cooking with rice was 12 DAF to 16 DAF. 9. Germination was possible after 10 DAF but we could get the normal roots from the seeds harvested after 14 DAF.

• Asian Journal of Turfgrass Science
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• v.9 no.2
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• pp.137-157
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• 1995

Some indices of species of the Arundinella hirta communities in Cheolwon area were studied and the following results were obtained. The vegetational date was analyzed from six grass stands selected with the distance away from the DMZ in Cheolwon area. Species diversity was higher in the stand away from the DMZ than in that neat to it. All six stands are not considered to be free of disturbance. Species composition of the stands might be related to the degree of disturbance. It can be said that the destruction of the vegetation by periodical fire or cutting simplifies the species composition. In the rear district the human inter ference is supposed to be more frequent. But it is supposed to be slight in near the DMZ and that the degree of disturbance is not so destructive. This could be the explanation of high diversity in these stands.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.1
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• pp.1-7
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• 2013

The purpose of this study was to provide basic information for breeding cowpeas (Vigna unguiculata L.) by investigating the crop characteristics of 245 accessions of cowpea collected in Korea and abroad. All specimens flowered within 41 to 50 days (51.5%) or 51 to 60 days (43.7%) of sowing and matured 21 to 30 days (53.9%) or 31 to 40 days (23.7%) from flowering. Thus, the total time from sowing to maturity was either 71 to 80 days (26.9%) or 81 to 90 days (23.4%) for all specimens. The accessions were classified into indeterminate type (72.7%), intermediate type (25.7%) and determinate type (1.6%) based on growth; prostrate type (78.8%) and erect type (21.2%) based on plant type; heart shape (98.4%) and lanceolate (1.6%) based on leaflet shape; and purple (85.2%), white (13.6%) and light green (1.2%) based on corolla color. The accessions were classified into brown (54.7%) and yellowish brown (37.6%) based on color at pod maturity; and downward (90.6%), middle (5.7%) and standing upright (3.7%) based on pod setting position. Seed coat color varied as 25.3% were brown, 23.3% were black, and 20.8% were white. Seed shape also varied as 66.9% were egg-shaped, 24.9% were rectangular and 8.2% were kidney-shaped. Pod lengths ranged from 10.1-20.0 cm and from 20.1-30.0 cm in 89.0% and 8.6% of specimens, respectively. There were 12.1-15.0, 9.1-12.0, and 15.1-18.0 seeds per pod in 62.0%, 25.7% and 9.1% of specimens, respectively. The weight of one hundred seeds ranged from 15.1-20.0 g (37.6%) and 10.1-15.0 g (28.6%). Seed yields per plant were 100.1-200.0 g (52.7%), less than 100 g (22.9%), and 200.1-300.0 g (15.9%). The starch content in the seeds of the seven selected resources ranged from 44.1 to 57.0% while the protein content ranged from 23.3 to 27.5% with significant differences. The sucrose content ranged from 1.46 to 2.03%, also with significant differences.

• Research in Plant Disease
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• v.17 no.2
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• pp.211-215
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• 2011

Cucumber mosaic virus (CMV)-like isolate was collected from Raphanus sativus (cv. Choon-hyang), which showed mosaic symptoms. The isolate was confirmed to a strain of CMV by host responses in Vigna unguiculata, Chenopodium amaranticolor and Gomphrena globosa, by viral genome composition with RT-PCR and PCR-RFLP, and by serological analysis. Symptom developed by the strain of CMV was severe in Nicotiana benthamiana, N. glutinosa, N. tabacum (cv. Samsun, cv. Xanthi), Cucumis melo (cv. Early hanover), Cucumis sativus (cv. White wonder), Capsicum annuum (cv. Chung-yang and cv. Geum-top), but mild symptom was developed in Raphanus sativus (cv. Choon-hyang), Brassica rapa ssp. pekinensis (cv. Bul-Am No. 3), and B. juncea (cv. Daenong Jukgot). Newly isolated strain of CMV could infect diverse crops including Solanaceae, Cucurbitaceae and Brassicaceae. We designated the new strain of CMV as Gn-CMV based on the novel infectivity of Brassicaceae. In double-stranded (ds) RNA analysis, Gn-CMV consisted of 3.3, 3.0, and 2.2 kb genomes likewise other strains of CMV. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) showed 28 kDa of the CMV coat protein. By restriction enzyme mapping using Cac8I, ClaI and MspI of RT-PCR products indicated that Gn-CMV belongs to CMV subgroup I.

• The Korean Journal of Food And Nutrition
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• v.31 no.4
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• pp.502-510
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• 2018

To begin with, this study identified and reviewed the proximate compositions, quality and physicochemical characteristics of three Korean cowpea (Vigna unguiculata L. Walp.) cultivars grown in two different seedling periods. The proximate compositions of the cowpea showed significant differences according to three cultivars (Seowon, Yeonboon and Okdang) and two different seeding periods (5 June and 5 July). Through this lens, the lightness, redness and yellowness of the cowpea cultivars increased slightly during the 5 July cultivation compared to the 5 June cultivation. This brings us to understand that the water binding capacity, water solubility index, and swelling power were significantly different among the cultivars. In this case, the water binding capacity of the cowpea was 108.87~143.19%, and decreased during the 5 July cultivation compared to the 5 June cultivation. As shown, the total polyphenol and flavonoid contents of cowpea showed significant difference according to cultivars. In this case, the total polyphenol content of 5 June and 5 July cultivation on the Seowon 4.17 and 4.12 mg GAE/g, respectively. In these terms, the DPPH radical scavenging activity of cowpea showed a significant difference according to the cultivars, and the Yeonboon and Okdang increased slightly during 5 July cultivation. The ABTS radical scavenging activity of the cowpea showed significant difference according to the cultivars, and all cultivars decreased slightly during the 5 July cultivation. In other words, the phenol contents and radical scavenging activity differ measurable depending on the cultivars and seeding periods. Therefore, it is necessary to study the proper cultivar and seeding time considering the environment of the applicable cultivation area.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.361-366
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• 1995

A virus was isolated from petunia (Petunia hybrida Vilm.) plants showing chlorotic ring spots on the leaves and color breaking on the flowers, and was identified as petunia asteroid mosaic virus (PAMV). Identification of the PAMV was established by host range test, electron microscopy, serological reaction, and physical properties of the virus. In the host range test, Nicotiana glutinosa, N. rustica, N. clevelandii, P. hybrida, Gomphrena globosa, and Chenopodium amaranticolor were systemically infected with the virus. The virus produced local lesions on inoculated leaves of N. tabacum‘Samsun’, N. tabacum‘Xanthi nc’, Datura stramonium, Vigna unguiculata‘White eye’, C. quinoa, Capsicum annuum, Vicia faba, and Lycopersicon esculentum‘Rutgers’. However, Cucurbita sativus and C. moschata did not show any symptoms. PAMV particles were isometric with 30 nm in diameter. The crude sap from G. globosa infected with the virus reacted positively with antiserum to tomato bushy stunt virus (TBSV) in agar gel double diffusion test. Thermal inactivation point of the virus was 8$0^{\circ}C$ and the virus retained its infectivity at the dilution of 10-4. Longevity in vitro of the virus was estimated longer than 35 days.

• The Plant Pathology Journal
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• v.29 no.4
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• pp.410-417
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• 2013

In 2011-2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in Riyadh region. Mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for Alfalfa mosaic virus (AMV) by ELISA were produced systemic mosaic on Vigna unguiculata and Nicotiana tabacum, local lesion on Chenopodium amaranticolor and C. quinoa. Vicia faba indicator plants that induce mosaic and mottle with AMV-Sagir isolate and no infection with AMV-Wadi aldawasser isolate. Approximately 700-bp was formed by RT-PCR using AMV coat protein specific primer. Samples from infected alfalfa gave positive results, while healthy plant gave negative result using dot blot hybridization assay. The nucleotide sequences of the Saudi isolates were compared with corresponding viral nucleotide sequences reported in GenBank. The obtained results showed that the AMV from Australia, Brazil, Puglia and China had the highest similarity with AMV-Sajer isolate. While, the AMV from Spain and New Zealaland had the lowest similarity with AMV-Sajer and Wadi aldawasser isolates. The data obtained in this study has been deposited in the GenBank under the accession numbers KC434083 and KC434084 for AMV-Sajer and AMV-Wadialdawasser respectively. This is the first report regarding the gnetic make up of AMV in Saudi Arabia.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.625-629
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• 1998

Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-poly-merase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 nm in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV--specfic oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var. hebecarpa, Rorippa cantoniensis and Thlaspi arvense.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.142-142
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• 2023

Cowpea(Vigna unguiculata L. Walp) has excellent physical properties and texture of starch, so it is mainly used in various ways for songpyeon sediment, rice-cake paste, and porridge. To utilize it as various processed products such as songpyeon sediment and cowpea milk, 'Okhyun', which has green cotyledons and black seed coats and can be harvested by combine was developed. A cowpea variety 'Okhyun' was improved from the cross between IT145379 and IT208081 at the JARES in 2022. 'Okhyun' has an erect plant with an intermediate plant habit, a light purple-colored corolla, and heart-shaped leaflets. 'Okhyun' has green cotyledons and black seed coats, and has straight black brown pods when mature. The stem length of 'Okhyun' is 47cm, and the weight of 100-seed weight was 12.9g, which was lower than the control variety 'Okdang'(16.6g). 'Okhyun' contains 25.8mg/100g of anthocyanin, 279mg/100g of total polyphenols, and 335mg/100g of total flavonoids, respectively. The average yield of 'Okhyun' was 1.97ton per hectare, 11% higher than that of the control cultivar 'Okdang'. 'Okhyun'' does not require the installation of an espalier-net net, so it is possible to harvest with a combine. In addition, 'Okhyun' variety with green cotyledons and a black seed coat is expected to contribute to developing regional specialties and various processed products.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.419-426
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• 1985

Two cowpea varieties, VITA #5 &Jungweon local var., had been sown at intervals of 15 days from May 1 to Aug. 29, and the following results were obtained. The earlier the two varieties had been sown, the shorter period from planting to first blooming and maturing, the more peduncles, the more pods per peduncle and plant, the heavier 100-grain weight, and the more grain yield we had. VITA #5 was earlier in maturity and higher in yielding performance than Jungweon local var., and both could not bloom in late sowing after Aug. 14. During the period of flowering and seed-setting, bad weather condition decreased the number of grains per pod. Limit sowing date of VITA #5 was Jun. 30 and that of Jungweon local var. was Jun. 15 in the middle part of Korea. Growing degree days (GDD) was available in cowpea.