• Applied Biological Chemistry
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• 제30권1호
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• pp.54-59
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• 1987

미숙잠두 종자의 추출물을 silica gel chromatography, Sephadex LH-20 chromatography, TLC, 역, 순상의 HPLC 등에 의해 분획정제하여 BR 활성물질을 한국산 벼를 이용한 생물검정법에 의해 검색한 결과, 2종의 BR의 존재가 인정되었으며, BR 보다 분자량과 극성이 작은 물질의 존재가 시사되었다.

• Applied Biological Chemistry
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• 제31권1호
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• pp.106-113
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• 1988

벼의 lamina inclination test를 이용하여 미숙 잠두종자 추출물에 포함되어 있는 BR 활성물질을 검색하였다. silica gel chromatraphy에 의해 극성이 다른 두 활성구를 얻었다. 극성이 높은 활성구를 정제하여 GC/MS, GC/SIM 분석에 의해 활성본체를 castasterone과 brassinolide로 동정하였다. 또, 극성이 낮은 활성구에서 활성본체를 결정으로 분리하고 MS, NMR등의 분석에 의해 이 물질이 4-Cl-IAA-Me임 을 밝혔다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1471-1478
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• 2015

The present study aimed to assess the effect of dietary substitution of soybean meal (SBM) with dehulled-micronized faba bean (Vicia faba var. minor) in guinea fowl broilers on their growth traits, carcass quality, and meat fatty acids composition. In this trial, 120 day-old guinea fowl keets were randomly assigned to two treatments which were fed from hatch to 12 weeks of age. Birds were fed two wheat middlings-based diets comprising of a control treatment which contained SBM (78.3 g/kg) and a test diet containing dehulled-micronized faba bean (130 g/kg) as the main protein source. Substituting SBM with faba bean had no adverse effect on growth traits, dressing percentage, or breast and thigh muscles relative weight of the guinea fowls. Conversely, a decrease (p<0.05) of abdominal fat was found in guinea fowls fed the faba bean-diet. Breast muscle of birds fed faba bean had higher $L^*$ score (p<0.05) and water-holding capacity (p<0.05) than the SBM control diet. Meat from guinea fowls fed faba bean had less total lipids (p<0.05) and cholesterol (p<0.01), and higher concentrations of phospholipids (p<0.01). Feeding faba bean increased polyunsaturated fatty acid concentrations in breast meat and decreased the saturated fatty acid levels. Moreover, dietary faba bean improved the atherogenic and thrombogenic indexes in guinea fowl breast meat. Results indicated that substitution of SBM with faba bean meal in guinea fowl diet can improve carcass qualitative traits, enhancing also meat lipid profile without negatively affecting growth performance.

• The Plant Pathology Journal
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• 제36권1호
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• pp.11-28
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• 2020

Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes. The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible. The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.

• 식물병연구
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• 제13권2호
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• pp.115-118
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• 2007

2004년부터 2006년까지 3년 동안 경남농업기술원 잠두재배포장에서 지제부위의 줄기와 꼬투리가 수침상으로 물러지고 썩으면서 그 부위에 흰색 곰팡이와 균핵이 생기고 시들어 죽는 증상이 발생하였다. 감자한천배지 상에서 균총은 흰색에서 연한 회색을 띄었다. 균핵은 구형, 타원형 또는 불규칙하며 검은색이었다. 자낭반은 컵모양이며 크기는 $5{\sim}10\;mm$이었으며 자낭은 원통형이고 크기는 $90{\sim}180{\times}12\;{\mu}m$이었다. 자낭포자는 타원형이며 무색으로 크기는 $8{\sim}12{\times}4{\sim}6\;{\mu}m$이었다. 균사생육 최적온도는 $25^{\circ}C$, 균핵형성 적온은 $20^{\circ}C$이었다. 이상과 같이 잠두에 발생한 병징, 병원균의 균학적 특징 및 병원성 검정 결과를 토대로 병원균을 Sclerotinia sclerotiorum (Lib.) do Bary으로 동정하였으며, 이 병을 잠두 균핵병으로 명명할 것을 제안한다.

• 식물병연구
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• 제9권1호
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• pp.21-24
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• 2003

2002년 5월 경남 남해군 창선면 잠두재배 농가 포장에서 B. cinerea에 의한 잠두 잿빛곰팡이병이 발생하였다. 병징은 잎에 발생하여 병든 부분이 수침상으로 물러지면서 썩으며 병반부위에 회색의 곰팡이가 많이 생기고 심할 경우 말라죽었다. 분생포자는 무색, 단포자이며 난형 또는 타원형으로 크기는 6~22$\times$4~16 $\mu\textrm{m}$였다. 분생자경 위에 분생포자가 아주 많이 형성되었다. 분생자경은 갈색으로 격막이 있고 폭은 15-37 $\mu\textrm{m}$였다. 감자한천배지(PDA) 에서 균사생육 적온은 2$0^{\circ}C$였으며, 균핵형성도 2$0^{\circ}C$에서 가장 많았다. 병원균은 Botrytis cinerea로 동정하였으며 잠두 잿빛곰팡이병으로 명명할 것을 제안한다.

• Mycobiology
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• 제29권4호
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• pp.198-204
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• 2001

Analysis of leaf exudates of Vicia faba using paper chromatography to identify individual amino acids and sugars qualitatively was investigated. The results revealed that the number of identified amino acids detected in the leaf exudates of the susceptible plants was more than those of resistant plants. The results also showed an increase in the number of amino acids exuded by infected leaves, but no marked difference in sugars of infected and non infected plants. Lithium chloride application led to decrease in amino acid and sugar contents. The number of amino acids and sugars was also decreased with leaf age. Botrytis fabae and the selected fungal species(Alternaria alternata, Fusarium oxysporum and Aspergillus niger) were used to show the effect of individual amino acid and sugar on their spore germination. It was observed that all amino acids stimulated the fungal spore germination except serine which inhibited its spore germination. In case of A. alternata, spore germination was stimulated by all amino acids except serine, alanine, glutamic acid, arginine and methionine which caused the inhibition. In case of F. oxysporum, aspartic and glutamic acids inhibited spore germination but the other amino acids stimulated its spore germination. Aspartic acid and phenyl alanine inhibited the spore germination of A. niger. All the identified sugars(galactose, glucose, fructose and rhamnose) stimulated spore germination of all tested fungi.

• The Plant Pathology Journal
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• 제23권1호
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• pp.31-33
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• 2007

Phytophthora rot on broad bean(Vicia faba) occurred in the experimental field at Gyeongsangnam-do Agricultural Research and Extension Services from 2004 to 2006. The fungus isolated from the diseased plants grew well on potato dextrose agar and showed an arachnoid or rosaceous colony pattern. Sporangia were conspicuously papillated, noncaducous, ovoid to globose, and $25-64{\times}18-44{\mu}m$ in size. Oogonia and oospores were spherical and measured as 20-32 ${\mu}m$ and 16-28 ${\mu}m$ in size, respectively. Oospores were relatively small and aplerotic. Antheridia were amphigynous, spherical, and unicellula. Chlamydospores were globose and 18-40 ${\mu}m$ in size. Optimum temperature for growth was about $28^{\circ}C$ on potato dextrose agar. The disease occurred in all parts of the plant including roots, stems, leaves and pods in the field. The symptoms similar to those of naturally infected plants were induced by artificial inoculation and the pathogen was re-isolated from the plant. On the basis of mycological and pathological characteristics, the causal pathogen of broad bean rot was identified as Phytophthora nicotianae. This is the first report of Phytophthora rot of broad bean caused by P. nicotianae in Korea.

• The Plant Pathology Journal
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• 제30권4호
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• pp.355-366
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• 2014

In this study, resistance responses were investigated during the interaction of Botrytis fabae with two faba bean cultivars expressing different levels of resistance against this pathogen, Nubaria (resistant) and Giza 40 (susceptible). Disease severity was assessed on leaves using a rating scale from 1 to 9. Accumulation levels of reactive oxygen species (ROS), lipid peroxidation and antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) were measured in leaf tissues at different times of infection. The expression profiles of two pathogenesis-related proteins (PRPs) encoded by the genes PR-1 and ${\beta}$-1,3-glucanase were also investigated using reverse transcription RT-PCR analysis. The accumulation of these defense responses was induced significantly in both cultivars upon infection with B. fabae compared with un-inoculated controls. The resistant cultivar showed weaker necrotic symptom expression, less ROS accumulation, a lower rate of lipid peroxidation and higher activity of the enzymatic ROS scavenging system compared with susceptible cultivar. Interestingly, ROS accumulated rapidly in the resistant leaf tissues and peaked during the early stages of infection, whereas accumulation was stronger and more intense in the susceptible tissues in later stages. Moreover, the response of the resistant cultivar to infection was earlier and stronger, exhibiting high transcript accumulation of the PR genes. These results indicated that the induction of oxidant/antioxidant responses and the accumulation of PRPs are part of the faba bean defense mechanism against the necrotrophic fungus B. fabae with a different intensity and timing of induction, depending on the resistance levels.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.109.1-109
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• 2002