• Title/Summary/Keyword: Vegetative buffer

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Biochemical Properties and Localization of the β-Expansin OsEXPB3 in Rice (Oryza sativa L.)

  • Lee, Yi;Choi, Dongsu
    • Molecules and Cells
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    • v.20 no.1
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    • pp.119-126
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    • 2005
  • ${\alpha}$-Expansins are bound to the cell wall of plants and can be solubilized with an extraction buffer containing 1 M NaCl. Localization of ${\alpha}$-expansins in the cell wall was confirmed by immunogold labeling and electron microscopy. The subcellular localization of vegetative ${\beta}$-expansins has not yet been studied. Using antibodies specific for OsEXPB3, a vegetative ${\beta}$-expansin of rice (Oryza sativa L.), we found that OsEXPB3 is tightly bound to the cell wall and, unlike ${\alpha}$-expansins, cannot be solubilized with extraction buffer containing 1 M NaCl. OsEXPB3 protein could only be extracted with buffer containing SDS. The subcellular localization of the OsEXPB3 protein was confirmed by immunogold labeling and electron microscopy. Gold particles were mainly distributed over the primary cell walls. Immunohistochemistry showed that OsEXPB3 is present in all regions of the coleoptile and root tissues tested.

Effects of Vegetative Buffers on Reducing Soil Erosion and Nutrient Loss of Highland Field in Korea (고랭지밭의 토양침식 저감을 위한 완충식생대의 효과)

  • Jin, Yong-Ik;Lee, Jeong-Tae;Lee, Gye-Jun;Hwang, Seon-Woong;Zhang, Yong-Seon;Park, Chang-Young;Seo, Myung-Chul;Ryu, Jong-Soo;Jeong, Jin-Cheol;Chung, Ill-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.231-238
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    • 2009
  • This study was carried out to investigate the effect of vegetative buffer to reduce runoff and soil and nutrient loss at highland agricultural area. The soil of experimental field was classified as Ungyo series (Fine, Humic Hapludults). An area of each field with lysimeter was $50m^2(width\;2.5m{\times}length\;20m)$ and was a gradient of 17%. Chinese cabbage (Brassica campestris L.) was cultivated by general management in each field. For establishing vegetative buffer, rye (Secalecereale L.), tall fescue (Festucaarundinacea Schreb) and orchard grass (Dactylis glomerata L.) were planted at the edge of field. Rye buffers were 1m, 2m and 4m wide. Both orchard grass and tall fescue buffers were 2m wide. Vegetative buffers were set up in September 2005 and chinese cabbage was planted in June 2006. Soil loss, runoff and nutrient loss were measured from June to August in 2006. Since the precipitation amount was heavy in July, amounts of runoff, soil erosion and nutrient loss were the highest in July during this study period. In comparison with control, vegetative buffers of rye 2m, orchard grass 2m and tall fescue 2m reduced runoff by 3%, 1% and 2%, respectively. In comparison among width of rye buffer, rye 1m, rye 2m, and rye 4m reduced by 1%, 4% and 13%, respectively. Vegetative buffers of rye 2m, orchard grass 2m and tall fescue 2m showed the reducing of soil loss by 59%, 46% and 28%, respectively. In comparison among width of rye buffer, the highest reducing effect of 88% was observed in 4m treatment. Additionally, vegetative buffer reduced N, P and K losses in runoff and eroded soil which were 10 to 54%, 7 to 24% and 11 to 21%, respectively. In different widths, wider vegetative buffer showed lower loss of N, P and K in runoff and eroded soil. As a result of this study, the vegetative buffer of rye was most effective for reducing runoff and soil loss in comparisons with other plants. In addition, wider range of buffers recommended for reducing runoff and soil loss, if possible.

Heat Resistance of Vegetative and Starved Listeria monocytogenes Sott A (Listeria monocytogenes Scott A의 세포상태에 따른 열 저항성)

  • 이신호;손수정
    • Microbiology and Biotechnology Letters
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    • v.21 no.2
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    • pp.176-180
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    • 1993
  • Survival and heat resistance of Listeria monocytogenes Scott A in various nutritional environments and cell type on stainless steel were determined. Viable cell of L. monocytogenes Scott A was most rapidly decreased in phosphate buffer among various media such as NSM (30 g TSB/1 l D.W.), LNM (2 g TSB/1 l D.W.) and phosphate buffer (pH=7) during incubation at 21 and 35C but survived for 15 days at 21C. Vegetative and starved L. monocytogenes Scott A were survived after heat treatment for 5 min at 65C while not detected at 72C.

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Effect of pH and Nisin on Heat Resistance of Listeria monocytogenes Scott A (Listeria monocytogenes의 열저항성에 미치는 pH와 Nisin의 효과)

  • 이신호;조현순
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.200-206
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    • 1993
  • The effect of pH (7, 5 and 4) and nisin (100 and 200IU/ml) on heat resistance of Listeria monocytogenes Scott A were determined using citrate-phosphate buffer system. Heat resistance of vegetative and starved cell was decreased as pH value was lower at 65 and 72C. Starved L. monocytogenes was more resistant than vegetative cell at both temperature. Heat resistance of vegetative and starved cell was decreased significantly with treatment of nisin. The effect of nisin was increased significantly at low pH(5, 4). Adherent microcolony was more resistant to heat and nisin than planktonic cell. Contamination of L. monocytogenes may be prevent by using nisin in food and food processing environments.

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The Resistance of Bacillus subtilis in Makgeolli to Hydrostatic Pressure (막걸리에 접종한 Bacillus subtilis의 초고압에 대한 저항력)

  • Lee, Eun-Jung;Kim, Joo-Sung;Oh, Se-Wook;Kim, Yun-Ji
    • Korean Journal of Food Science and Technology
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    • v.44 no.3
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    • pp.312-316
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    • 2012
  • In order to understand the effect of hydrostatic pressure (HP) on Bacillus subtilis isolated from makgeolli, the survival of B. subtilis after HP treatment (400 MPa for 5 min) in various substrates including phosphate buffer, tryptone soya broth at pH 7 and 4, and makgeolli at pH 4 was evaluated depending on bacterial forms (spores and vegetative cells) and adaptation conditions ($25^{\circ}C$ for 3 h, or $10^{\circ}C$ for 24 h). Spores were generally resistant to HP (<1 log reduction) regardless of conditions. In contrast, vegetative cells were generally susceptible to HP (up to 3 log reduction-except makgeolli) and were more susceptible after 3 h at $25^{\circ}C$ compared to 24 h at $10^{\circ}C$. In vegetative cells inoculated makgeolli (7 log CFU/mL), the colonies were not detected after 24 h at $10^{\circ}C$. Consequently, B. subtilis in makgeolli easily existed as spores and the spores were resistant to HP. Results demonstrate that HP was more promising in the inactivation of vegetative cells.

In Vitro Dissolution and Proteolytic Activation of $\delta$-endotoxin and Antigenic Expression Pattern of Bacillus thuringiensis subsp, sotto (Bacillus thuringiensis subsp. sotto의 내독소 결정체 용해 과정 및 활성기작과 항원 발현 양상)

  • Nam, Gi-Bum;Cho, Jae-Min;Hong, Soon-Bok;Lee, Hyung-Hoan;Cho, Myung-Hwan
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.730-736
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    • 1995
  • The solubilization and proteolytic process of $\delta $-endotoxin was analvsed to compare the biochemical property of the toxin isolated from B. thuringiensis subsp. sotto. The purified crystals were dissolved in 50 mM carbonate buffer containing 10 mM dithiothreitol at pH 10 for various times. The electrophoretic pattern showed that a rapid disappearance of 138 kDa protein band. This disappearance of protein with high molecular weight was accompanied by the appearance of new protein fragment with 104 kDa, 60 kDa, and 25 kDa. For proteolvtic processing, the soluble crystals were digested with trypsin for various times. The soluble crystal protein of 104 kDa was completely disappeared. However, the protein fragment of 60 kDa and 25 kDa still remained after complete proteolysis. The comparative immunoblot analysis showed that the antiserum against intact crystals showed strong immunoreactivity to the homologous inclusion protein of 138 kDa, 104 kDa, and 25 kDa, and to the intact spores of 221 kDa and 138 kDa, but not to the vegetative cell homogenate. The sera against crystals and spores had no immunoreactivity to the vegetative cell homogenate.

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Purification and risk assessment of Bacillus thuringiensis Vip3Aa protein against Apis mellifera (Bacillus thuringiensis 유래 Vip3Aa 단백질 순수분리 및 꿀벌 (Apis mellifera)에 대한 위해성평가)

  • Jung, Young Jun;Yoo, Su-Hyang;Lee, Jung Ro
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.585-591
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    • 2019
  • Most insect-resistant LMOs have been produced by applying Cry and Vip3Aa proteins. Vip3Aa protein is activated during the vegetative stage of Bacillus thuringensis (Bt) and the inhibitory activity of the Vip3Aa protein against pathogenic attacks from lepidopteran insect species is well known. However, a risk assessment of the Vip3Aa protein compared to the Cry protein has not been conducted in South Korea. This study demonstrates a possible risk assessment method for Vip3Aa protein against honeybees (Apis mellifera). For the risk assessment of the protein, we purified the recombinant Vip3Aa protein in Escherichia coli. The survival rate and symptoms of general intoxication of 4 months honeybees were measured after Vip3Aa exposure. These results indicated that there was no significant difference in the survival rate and the symptom between Vip3Aa and the control buffer. In this study, we established standard methods of Vip3Aa protein purification and oral adult toxicity test using A. mellifera as an LMO risk assessment technique for preserving the natural ecosystem of South Korea.

Development of Guidelines for Animal Waste Land Application to Minimize Water Quality Impacts (축산분뇨 농지환원을 위한 적정관리방안)

  • 홍성구
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.44 no.5
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    • pp.136-146
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    • 2002
  • Land application of manure compost is considered one of the widely-used animal waste management practices. Many livestock farms adopt composting for their animal waste disposal and apply the compost to crop fields. While standard rates have been established based on researches with respect to land application of manure compost recently, there have been few discussions on water quality impact of the application. Water quality impact should be taken into account in land applications of manure compost. In this study, management practices were proposed based on the investigation of water quality of leachate from manure compost under rainfall simulation, field studies, and monitoring runoff water quality from farm fields after land application of animal waste. The concentrations of major water quality parameters of the leachate were significantly high, whereas those of runoff from soils after tillage for soil incorporation, were not affected by the application based on a series of experiments. Runoff water from farm fields after land application also showed high concentrations of pollutants. Appropriate management practices should be employed to minimize pollutant loading from manure applied fields. Proposed major management practices include 1) application of recommended amounts, 2) proper tillage for complete soil and manure incorporation, 3) field management to prevent excessive soil erosion, 4) complete diversion of inflow into the field from outside, 5) implementation of vegetative buffer strips near boundaries, and 6) prevention of direct discharge of runoff water front fields Into streams.

Management Strategies to Conserve Soil and Water Qualities in the Sloping Uplands in Korea (한국의 경사지 밭의 토양 및 물의 보전 관리 전략)

  • Yang, Jae-E.;Ryu, Jin-Hee;Kim, Si-Joo;Chung, Doug-Young
    • Korean Journal of Agricultural Science
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    • v.37 no.3
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    • pp.435-449
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    • 2010
  • Soils in the sloping uplands in Korea are subject to intensive land use with high input of agrochemicals and are vulnerable to soil erosion. Development of the environmentally sound land management strategy is essential for a sustainable production system in the sloping upland. This report addresses the status of upland agriculture and the best management practices for the uplands toward the sustainable agriculture. More than 60% of Korean lands are forest and only 21% are cultivating paddy and upland. Uplands are about 7% of the total lands and about 62% of the uplands are in the slopes higher than 7%. Due to the site-specificity of the upland, many managerial and environmental problems are occurring, such as severe erosion, shallow surface soils with rocky fragments, and loadings of non-point source (NPS) contaminants into the watershed. Based on the field trials, most of the sloping uplands were classified as Suitability Class III-V and the major limiting factor was slope and rock fragments. Due to this, soils were over-applied with N fertilizer, even though N rate was the recommendation. This resulted in decreases in yield, degradation of soil quality and increases in N loading to the leachate. Various case studies drew management practices toward sustainable production systems. The suggested BMP on the managerial, vegetative, and structural options were to practice buffer strips along the edges of fields and streams, winter cover crop, contour and mulching farming, detention weir, diversion drains, grassed waterway, and slope arrangement. With these options, conservation effects such as reductions in raindrop impact, flow velocity, runoff and sediment loss, and rill and gully erosion were observed. The proper management practice is a key element of the conservation of the soil and water in the sloping upland.

A Study on Non-point Source Pollutants from Pavement of Coastal Area and Guidance for Selecting BMP (연안지역 포장면 비점오염물질 유출 및 최적관리방안 시설의 선정을 위한 가이던스에 관한 연구)

  • Ko, Woong;Park, Kisoo;Chen, Yaoping;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.15 no.4
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    • pp.543-553
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    • 2013
  • In Korea, there have been many studies and progresses on various types of pilot scale or commercialized field scale Best Management Practices(BMP) during the last 10 years. Runoff characteristics of diffuse pollutants from different land uses are well identified and documented. However, for the coastal area connected directly with shoreline, runoff patterns and management schemes of non-point pollution were not fully studied. Recently, coastal area is emerging as a new development axis especially in west coast side of Korea such as Incheon city and Chungnam province, thus urbanized area rapidly increased but there are no buffer zones and BMP facilities to aim at preventing direct discharge of the first flush into coastal sea and beaches. In this study, parking area in Deacheon harbor, Boryeong City in Chungnam Province was selected and rainfall runoff was monitored for two year period in order to examine run-off features from which proper type of BMP suitable for coastal area is proposed. Coastal area usually has a low ground water level and consists of plain land, so that large scale BMP such as storm water retention pond and wetland requiring great excavation works is not best management plan. In addition, monitoring study shows that storm water from the paved parking area has a high salinity compared with those in storm water from the inland. High salinity is detrimental for the vegetation. Therefore, BMP employing least vegetative cover and also in terms of maintenance is a good option such as infiltration trench and porous pavement.