• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.4B
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• pp.771-782
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• 2000

In this paper, an H.263 video codec is implemented by adopting the concept of hardware and software co-design. Each module of the codec is investigated to find which approach between hardware and software is better to achieve real-time processing speed as well as flexibility. The hardware portion includes motion-related engines, such as motion estimation and compensation, and a memory control part. The remaining portion of theH.263 video codec is implemented in software using a RISC processor. This paper also introduces efficient design methods for hardware and software modules. In hardware, an area-efficient architecture for the motion estimator of a multi-resolution block matching algorithm using multiple candidates and spatial correlation in motion vector fields (MRMCS), is suggested to reduce the chip size. Software optimization techniques are also explored by using the statistics of transformed coefficients and the minimum sum of absolute difference (SAD)obtained from the motion estimator.

• Development and Reproduction
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• v.17 no.4
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• pp.379-387
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• 2013

Embryonic stem (ES) cells can self-renew and differentiate to various cells depending on the culture condition. Although ES cells are a good model for cell type specification and can be useful for application in clinics in the future, studies on ES cells have many experimental restraints including low transfection efficiency and transgene expression. Here, we observed that transgene expression after transfection was enhanced by treatment with histone deacetylse (HDAC) inhibitors such as trichostatin A, sodium butyrate, and valproic acid. Transfection was performed using conventional transfection reagents with a retroviral vector encoding GFP under the control of CMV promoter as a reporter. Treatment of ES cells with HDAC inhibitors after transfection increased population of GFP positive cells up to 180% compared with untreated control. ES cells showed normal expression of stem cell markers after treatment with HDAC inhibitors. Transgene expression was further enhanced by modifying transfection procedure. GFP positive cells selected after transfection were proved to have the stem cell properties. Our improved protocol for enhanced gene delivery and expression in mouse ES cells without hampering ES cell properties will be useful for study and application of ES cells.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1529-1540
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• 2011

Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is relatively nil. In the present study, we have validated the interfering effects of siRNA (small interfering RNA) using both quantitative and qualitative gene silencing in buffalo granulosa cells. Qualitative gene knockdown was validated using a fluorescent vector, enhanced green fluorescence protein (EGFP) and fluorescently labeled siRNA (Cy3) duplex. While quantitatively, siRNA targeted against the luciferase and CYP19 mRNA was used to validate the technique. CYP19 gene, a candidate fertility gene, was selected as a model to demonstrate the technique optimization. However, to sustain the expression of CYP19 gene in culture conditions using serum is difficult because granulosa cells have the tendency to luteinize in presence of serum. Therefore, serum free culture conditions were optimized for transfection and were found to be more suitable for the maintenance of CYP19 gene transcripts in comparison to culture conditions with serum. Decline in fluorescence intensity of green fluorescent protein (EGFP) was observed following co-transfection with plasmid generating siRNA targeted against EGFP gene. Quantitative decrease in luminescence was seen when co-transfected with siRNA against the luciferase gene. A significant suppressive effect on the mRNA levels of CYP19 gene at 100 nM siRNA concentration was observed. Also, measurement of estradiol levels using ELISA (enzyme-linked immunosorbent assay) showed a significant decline in comparison to control. In conclusion, the present study validated gene silencing using RNAi in cultured buffalo granulosa cells which can be used as an effective tool for functional analysis of target genes.

• Composites Research
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• v.24 no.2
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• pp.38-45
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• 2011

In this paper, we present an optimization method for the single Dallenbach-layer type microwave absorbers composed of E-glass fabric/epoxy composite laminates. The composite prepreg containing carbon nanotubes (CNT) was used to control the electrical property of the composites laminates. The design technology using the genetic algorithm was used to get the optimal thicknesses of the laminates and the filler contents at various center frequencies, for which the numerical model of the complex permittivity of the composite laminate was incorporated. In the optimal design results, the content of CNT increased in proportion to the center frequency, but, on the contrary, the thickness of the microwave absorbers decreased. The permittivity and reflection loss are measured using vector network analyzer and 7 mm coaxial airline. The influence of the mismatches in between measurement and prediction of the thickness and the complex permittivity caused the shift of the center frequency, blunting of the peak at the center frequency and the reduction of the absorbing bandwidth.

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.388-393
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• 2017

We report an Agrobacterium-mediated transformation procedure optimized for 'Kyoho' that is a major table grapevine cultivar in Korea, and its transgenic plants with antifreeze protein gene of carrot (DcAFP). The full length of DcAFP coding region in accordance with the previous report was isolated from young leaves of carrot and recombined into a plant transformation vector. Ethylene inhibitors such as silver nitrate and aminoethoxyvinylglycin (AVG) supplemented in a co-cultivation medium distinctly increased frequency of shoot regeneration when explants were sub-cultured in a selection medium: particularly ten-fold higher in treatment with 0.1 mg/L AVG than one without ethylene inhibitor. Among various antibiotics and their concentrations, the combination of 150 mg/L cefotaxime plus 150 mg/L $Clavamox^{TM}$ was selected for elimination of Agrobacterium cells in addition to minimization of adverse effect on shoot regeneration, while 50 mg/L kanamycin monosulfate effectively suppressed regeneration of non-transgenic shoots. Applying the elucidated culture condition, we finally obtained a total of 5 transgenic 'Kyoho' plantlets with DcAFP, of which integration with the grapevine genome and transcription was confirmed by nucleic acid analyses.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.6B
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• pp.1183-1190
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• 1999

This paper presents an efficient hardware architecture to improve the frame memory interface occupying the largest hardware area together with motion estimator in implementing MPEG-2 video encoder as an ASIC chip. In this architecture, the memory size for internal data buffering and hardware area for frame memory interface control logic are reduced through the efficient memory map organization of the external SDRAM having dual bank and memory access timing optimization between the video encoder and external SDRAM. In this design, 0.5 m, CMOS, TLM (Triple Layer Metal) standard cells are used as design libraries and VHDL simulator and logic synthesis tools are used for hardware design add verification. The hardware emulator modeled by C-language is exploited for various test vector generation and functional verification. The architecture of the improved frame memory interface occupies about 58% less hardware area than the existing architecture[2-3], and it results in the total hardware area reduction up to 24.3%. Thus, the (act that the frame memory interface influences on the whole area of the video encoder severely is presented as a result.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.66 no.9
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• pp.1392-1401
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• 2017

In this study, the design methodology of pattern classification is introduced for avoiding faults through partial discharge occurring in the power facilities and local sites. In order to classify some partial discharge types according to the characteristics of each feature, the model is constructed by using the Radial Basis Function Neural Networks(RBFNNs) and Particle Swarm Optimization(PSO). In the input layer of the RBFNNs, the feature vector is searched and the dimension is reduced through Principal Component Analysis(PCA) and PSO. In the hidden layer, the fuzzy coefficients of the fuzzy clustering method(FCM) are tuned using PSO. Raw datasets for partial discharge are obtained through the Motor Insulation Monitoring System(MIMS) instrument using an Epoxy Mica Coupling(EMC) sensor. The preprocessed datasets for partial discharge are acquired through the Phase Resolved Partial Discharge Analysis(PRPDA) preprocessing algorithm to obtain partial discharge types such as void, corona, surface, and slot discharges. Also, when the amplitude size is considered as two types of both the maximum value and the average value in the process for extracting the preprocessed datasets, two different kinds of feature datasets are produced. In this study, the classification ratio between the proposed RBFNNs model and other classifiers is shown by using the two different kinds of feature datasets, and also we demonstrate the proposed model shows superiority from the viewpoint of classification performance.

• Journal of Korea Multimedia Society
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• v.15 no.4
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• pp.485-491
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• 2012

This paper proposes 'Coding Unit Tree' based on quadtree efficiently with motion vector to represent splitting information of a Coding Unit (CU) in HEVC. The new international video coding, High Efficiency Video Coding (HEVC), adopts various techniques and new unit concept: CU, Prediction Unit (PU), and Transform Unit (TU). The basic coding unit, CU is larger than macroblock of H.264/AVC and it splits to process image-based quadtree with a hierarchical structure. However, in case that there are complex motions in CU, the more signaling bits with motion information need to be transmitted. This structure provides a flexibility and a base for a optimization, but there are overhead about splitting information. This paper analyzes those signals and proposes a new algorithm which removes those redundancy. The proposed algorithm utilizes a type code, a dominant value, and residue values at a node in quadtree to remove the addition bits. Type code represents a structure of an image tree and the two values represent a node value. The results show that the proposed algorithm gains 13.6% bit-rate reduction over the HM-1.0.

• Research in Plant Disease
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• v.18 no.3
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• pp.231-235
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• 2012

Indian citrus ring spot virus (ICRSV) is known to cause a serious disease to citrus, especially to Kinnow mandarin, the popular cultivated citrus species in India. In this study, we developed diagnostic systems based on enzyme-linked immunosorbent assay (ELISA). In order to generate antibodies against ICRSV coat protein, we overexpressed the coat protein in Escherichia coli using the pET15b expression vector containing an optimized ICRSV coat protein gene. The recombinant ICRSV coat protein was overexpressed as soluble form at $37^{\circ}C$ upon IPTG induction. The protein was purified to 95% in purity by Ni-NTA column chromatography. The purified protein was immunized to rabbit for the generation of polyclonal antibody (PAb). The PAb showed a specific immunoreaction to recombinant ICRSV coat protein in western blot analysis and ELISA. Diluted rabbit antisera (10,000 fold) could detect less than 10 ng and 5 ng of the target protein in western blot and ELISA analysis, respectively.

• Journal of Korea Multimedia Society
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• v.10 no.11
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• pp.1530-1537
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• 2007

In this paper, we implemented a speech emotion recognition system that can distinguish human emotional states from recorded speech captured by a single microphone and classify them into four categories: neutrality, happiness, sadness and anger. In general, a speech recorded with a microphone contains background noises due to the speaker environment and the microphone characteristic, which can result in serious system performance degradation. In order to minimize the effect of these noises and to improve the system performance, a MA(Moving Average) filter with a relatively simple structure and low computational complexity was adopted. Then a SFS(Sequential Forward Selection) feature optimization method was implemented to further improve and stabilize the system performance. For speech emotion classification, a SVM pattern classifier is used. The experimental results indicate the emotional classification performance around 65% in the computer simulation and 62% on the ARM platform.