• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.4B
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• pp.771-782
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• 2000

In this paper, an H.263 video codec is implemented by adopting the concept of hardware and software co-design. Each module of the codec is investigated to find which approach between hardware and software is better to achieve real-time processing speed as well as flexibility. The hardware portion includes motion-related engines, such as motion estimation and compensation, and a memory control part. The remaining portion of theH.263 video codec is implemented in software using a RISC processor. This paper also introduces efficient design methods for hardware and software modules. In hardware, an area-efficient architecture for the motion estimator of a multi-resolution block matching algorithm using multiple candidates and spatial correlation in motion vector fields (MRMCS), is suggested to reduce the chip size. Software optimization techniques are also explored by using the statistics of transformed coefficients and the minimum sum of absolute difference (SAD)obtained from the motion estimator.

• Journal of Periodontal and Implant Science
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• v.32 no.3
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• pp.603-614
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• 2002

Human gingival fibroblasts have proven to useful as a species specific cell culture system in various system on periodontal disease and regeneration. However, their use is limited, since they are hard to obtain and lifespan is short due to replicative senescence. To overcome these disadvantages, we transfected primary human gingival fibroblasts by the E6 and E7 genes of the Human papilloma virus(HPV) 16. The full length of HPV 16 E6 and E7 was cloned from the pBR322 into BamHl and Sal I of a pBabe vector including hygromycin B resistance. Before pBabeE6/E7 plasmid transfection, peak 8 GFP including G418 resistance was transfected into primary GF to check the transfection efficency. PBabe E6/E7 plasmid was transfected using Lipofectamine plus following manufacter's instruction into primary normal human gingival fibroblasts in 60mm dishes with FBS free DMEM. After 2 days of transfection, the cells were treated with hygromycin for 2 weeks until the transfected control cells died. The resulting hygromycin resistant colonies were pooled, and clonned, and sucessful　transfection was established for immortalized gingival fibroblast cell lines. Immoralized GF cells showed stellate shape, that is similar to that of orange grains, and more rapid growth and higher proliferation than that of primary gingival fibroblasts. This cell lines overcame crisis and could be cultured over 30 subcultured, could be use for three dimentional culture, epithelial-mesenchymal interaction study.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.33 no.3
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• pp.401-410
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• 2023

In this paper, we propose optimization methods for implementing SPARKLE, one of the NIST LWC finalists, on a 64-bit ARMv8 processor. The proposed methods consist of two approaches: an implementation using ARM A64 instructions and another using NEON ASIMD instructions. The A64-based implementation is optimized by performing register scheduling to efficiently utilize the available registers on the ARMv8 architecture. By utilizing the optimized A64-based implementation, we can achieve speeds that are 1.69 to 1.81 times faster than the C reference implementation on a Raspberry Pi 4B. The ASIMD-based implementation, on the other hand, optimizes data by parallelizing the ARX-boxes to perform more than three of them concurrently through a single vector instruction. While the general speed of the optimized ASIMD-based implementation is lower than that of the A64-based implementation, it only slows down by 1.2 times compared to the 2.1 times slowdown observed in the A64-based implementation as the block size increases from SPARKLE256 to SPARKLE512. This is an advantage of the ASIMD-based implementation. Therefore, the ASIMD-based implementation is more efficient for SPARKLE variant block cipher or permutation designs with larger block sizes than the original SPARKLE, making it a useful resource.

• The Korean Journal of Food And Nutrition
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• v.15 no.2
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• pp.112-118
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• 2002

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by matting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB(spo5)1. We futher analyzed six recombinant plasmids, pDB(spo5)2, pDB(spo5)3, pDB(spo5)4, pDB(spo5)5, pDB (spo5)6, pDB(spo5)7 and found each of these plasmids is able to rescue the spo5-2, spo5-3, spo5-4, spo5-5, spo5-6, spo5-7 mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB(spo5)1, and pDB(spu5)Rl contained the spo5 gene. Transcripts of spo5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 2.5kb were detected with 5kb Hind Ⅲ fragment containing a part of the spo5 gene as a probe. The small mRNA(2.5kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2kb) was produced constitutively. Appearance of a 2.5kb spo5-mRNA depends upon the function of the meil, mei2 and mei3 genes.