• The Journal of Information Technology
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• v.7 no.2
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• pp.93-104
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• 2004

Sports scene is characterized by large amount of global motion due to pan and zoom of camera motion, and includes many small objects moving independently. Some short period of sports games is thrilling to televiewers, and important to producers. At the same time that kinds of scenes exhibit exceptionally dynamic motions and it is very difficult to analyze the motions with conventional algorithms. In this thesis, several algorithms are proposed for global motion analysis on these dynamic scenes. It is shown that proposed algorithms worked well for motion compensation and panorama synthesis. When cascading the inter frame motions, accumulated errors are unavoidable. In order to minimize these errors, interpolation method of motion vectors is introduced. Affined transform or perspective projection transform is regarded as a square matrix, which can be factorized into small amount of motion vectors. To solve factorization problem, we preposed the adaptation of Newton Raphson method into vector and matrix form, which is also computationally efficient. Combining multi frame motion estimation and the corresponding interpolation in hierarchical manner enhancement algorithm of motion parameters is proposed, which is suitable for motion compensation and panorama synthesis. The proposed algorithms are suitable for special effect rendering for broadcast system, video indexing, tracking in complex scenes, and other fields requiring global motion estimation.

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.7-12
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• 2001

An antiserum against the carrot HSP17 (17 KDa heat shock protein) was raised using the HSP17 purified after being expressed in a recombinant E.coli in order to develop an assay system for thermotolerance in crops. The DCHsp17.7 including the coding sequence corresponding to a carrot HSP17 protein was recombined within pET-32(b) vector and achieved a maximum expression in 4 hours after an induction in E.coli. The purified DCHsp17.7 was used as an antigen to generate the corresponding antibody. The polyclonal antiserum was confirmed for it's specificity only to the low molecular weight (1mw) HSP. Besides, the possibilities to use the antiserum to interact with 1mwHSPs from other plants such as rice, cucumber, tomato, and hot pepper were examined to be plausible. To reveal any specific correlation between the amounts of 1mwHSP expressed upon HS conditions and an acquisition of thermotolerance two different approaches have been applied. first, it has been shown that only the pre-HS conditions inducing the synthesis of HSP17 allowed for the seedlings to achieve an thermotolerance and to survive the following lethal condition. Second, a western analysis using 15 different collected lines of hot peppers was performed to distinguish each other in terms of the amount of 1mwHSP. The results indicated that all 14 hot pepper lines were able to synthesize HSPs in response to an exposure to HS conditions and the amounts of the proteins synthesized at different HS temperatures were variable among the lines. There are several different patterns of 1mwHSP synthesized as a function of temperature increase observed and their correlation to physiological aspects of thermotolerance remains to be analyzed.

• Journal of Biosystems Engineering
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• v.8 no.2
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• pp.26-38
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• 1983

Binder harvesters introduced to Korea were originally designed to be used for Japonica varieties which are highly resistant to shattering. In order to improve the performance of the binder to Indica varieties which are easily shattered and have shorter stem, mechanical modifications of the binder are inevitable. Shattering losses of the binder can be classified into two major parts; one incurred before and one after binding operations. The latter has been evaluated as great as the former. Previous studies indicated that the high discharge losses resulted from a great impact force of the discharge arm on the rice bundle during the discharge process. This study was intended to theoretically analyze the discharge mechanism of four-bar linkage. For this purpose, two commercially available binder harvesters having a four-bar linkage as a discharge mechanism were analyzed. Using the results from the motion analysis and the other structural constraints of the machines, they were modified and experimentally compared with the machines without modification to see whether any decrease in grain losses was obtained. The results obtained in this study are summarized as follows: 1. The path, velocity and acceleration of discharge arm were computer analyzed by vector analysis. Using results of the analysis and intrinsic constraints of the binder, discharge mechanism was modified to reduce the impact force on bundle by discharge arm in the range where the discharge performance was not deteriorated. This modification of the discharge mechanism could be done with an aid of four-bar linkage synthesis technique. As a result, average velocity and acceleration of the discharge arm during the discharge process were reduced respectively by 19 percent and 33 percent for binder A, and 17 percent and 35 percent for binder B. 2. Through the modification of the discharge mechanism, discharge losses of binder A were reduced by 42-56 percent for Milyang 23, Poongsan and Hangang chal, and discharge losses of binder B were reduced by 13-20 percent for Milyang 23 and Poongsan. 3. Discharge losses were decreased as the bundle size became larger and the size effect on the decrease rate appeared more significant in the binders with modifications than in those without modifications.

• The Plant Pathology Journal
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• v.17 no.5
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• pp.300-304
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• 2001

Apple is the most economically important fruit in Korea. The suspected viroid disease of dapple apple was found in apple fruits cultivated in Kyungpook province. Symptoms begin in mid-July as small circular spots, which stand out against the background color on the young fruit. Dappling of the fruit becomes more intense and easier to detect as the fruit approaches maturity; the affected spots remain yellowish as the fruit matures. no leaf or bark syndromes have been associated with this disease. The infected fruits are downgraded considerably during quality grading. The low molecular weight RNA containing viroid RNA molecules were extracted from the peels of the apples with dapple symptoms. The RNA molecules were extracted from the apples using Qiagen column chromatography. The purified RNAs were used for the synthesis of cDNA with RT-PCR. The PCR products were then ligated into a pGEM-T Easy vector, cloned and sequenced. The sequence of the viroid RNA molecule shows 331 nucleotides with one base difference ("G" insertion between the position of 133 and 134) compared with that of the Apple scar skin viroid (ASSVd) reported by Hashimoto and Koganezawa in Japan. This is the first report on the occurrence of the ASSVd in apple trees cultivated in Korea, as well as the identification of a new Korean strain of the ASSVd.the ASSVd.

• BMB Reports
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• v.39 no.3
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• pp.329-334
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• 2006

RNAi (RNA interference) has become a popular means of knocking down a specific gene in vivo. The most common approach involves the use of chemically synthesized short interfering RNAs (siRNAs), which are relatively easy and fast to use, but which are costly and have only transient effects. These limitations can be overcome by using short hairpin RNA (shRNA) expression vectors. However, current methods of generating shRNA expression vectors require either the synthesis of long (50-70 nt) costly oligonucleotides or multi-step processes. To overcome this drawback, we have developed a one-step short-oligonucleotides-based method with preparation costs of only 15% of those of the conventional methods used to obtain essentially the same DNA fragment encoding shRNA. Sequences containing 19 bases homologous to target genes were synthesized as 17- and 31-nt DNA oligonucleotides and used to construct shRNA expression vectors. Using these plasmids, we were able to effectively silence target genes. Because our method relies on the onestep ligation of short oligonucleotides, it is simple, less error-prone, and economical.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.6B
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• pp.1183-1190
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• 1999

This paper presents an efficient hardware architecture to improve the frame memory interface occupying the largest hardware area together with motion estimator in implementing MPEG-2 video encoder as an ASIC chip. In this architecture, the memory size for internal data buffering and hardware area for frame memory interface control logic are reduced through the efficient memory map organization of the external SDRAM having dual bank and memory access timing optimization between the video encoder and external SDRAM. In this design, 0.5 m, CMOS, TLM (Triple Layer Metal) standard cells are used as design libraries and VHDL simulator and logic synthesis tools are used for hardware design add verification. The hardware emulator modeled by C-language is exploited for various test vector generation and functional verification. The architecture of the improved frame memory interface occupies about 58% less hardware area than the existing architecture[2-3], and it results in the total hardware area reduction up to 24.3%. Thus, the (act that the frame memory interface influences on the whole area of the video encoder severely is presented as a result.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.505.1-505
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• 1986

The gene for iso-1-cytochrome c for Saccharomyces cerevisiae was recloned into a pSP65 vector containing an active bacteriophage SP6 promoter. The iso-1-cytochrome c gene was cloned as an 856 bp Xho 1-Hind III fragment. When the resulting plasmid was digested at the Hind 111 site 279 bases downstream from the termination codon of the gene and transcribed in vitro using SP6 RNA polymerase, full length transcripts were produced. The SP6 iso-1-cytochrome c mRNA was translated using a rabbit reticulocyte lysate system and the protein products analyzed on SDS polyacrylamide gels. One major band was detected by autofluorography. This band was found to have a molecular weight of 12,000 Da and coincided with the Coomassie staining band of apocytochrome c from S. cerebisiae. The product was also shown to be identical with that of standard yeast apocytochrome c on an isoelectric focusing gel. The in vitro synthesized iso-a-cytochrome c was methylated by adding partially purified S-adenosyl-L-methionine . protein-lysine N-methyltransferase (Protein methylase III; EC 2.1.1.43) from S. cerevisiae along with S-adenosyl-L-methionine to the in vitro translation mixtures. The methylation was shown to be inhibited by the addition of the methylase inhibitor S-adenosyl-L-homocysteine or the protein synthesis inhibitor pu omycin. The methyl derivatives in the protein were identified as $\varepsilon$-N-mono, di and trimethyllysine by amino acid analysis. The molar ratio of methyl groups incorporated to that of cytochrome c molecules synthesized showed that 23% of the translated cytochrome c molecules were methylated by protein methylase III.

• Journal of Microbiology and Biotechnology
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• v.2 no.4
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• pp.243-247
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• 1992

The thr operon of Escherichia coli TF427, an $\alpha$-amino-$\beta$-hydroxyvaleric acid (AHV)-resistant threonine overproducer, was cloned in a pBluescriptII $KS^＋$ plasmid by complementation of E. coli mutants. All clones contained a common 8.8 kb HindIII-generated DNA fragment and complemented the thrA, thrB, and thrC mutants by showing that these clones contained the whole thr operon. This thr operon was subcloned in the plasmid vectors pBR322, pUC18, and pECCG117, an E. coli/Corynebacterium glutamicum shuttle vector, to form recombinant plasmids pBTF11, pUTF25 and pGTF18, respectively. The subcloned thr operon was shown to be present in a 6.0 kb insert. A transformant of E. coli TF125 with pBTF11 showed an 8~11 fold higher aspartokinase I activity, and 15~20 fold higher L-threonine production than TF125, an AHV-sensitive methionine auxotroph. Also, it was found that the aspartokinase I activity of E. coli TF125 harboring pBTF11 was not inhibited by threonine and its synthesis was not repressed by threonine plus isoleucine.

• The Journal of the Korea Contents Association
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• v.16 no.6
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• pp.9-18
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• 2016

This paper proposes a multi-view Wyner-Ziv Video coding scheme based on spatio-temporal adaptive estimation. The proposed algorithm is designed to search for a better estimated block with joint bi-directional motion estimation by introducing weights between temporal and spatial directions, and by classifying effectively the region of interest blocks, which is based on the edge detection and the synthesis, and by selecting the reference estimation block from the effective motion vector analysis. The proposed algorithm exploits the information of a single frame viewpoint and adjacent frame viewpoints, simultaneously and then generates adaptively side information in a variety of closure, and reflection regions to have a better performance. Through several simulations with multi-view video sequences, it is shown that the proposed algorithm performs visual quality improvement as well as bit-rate reduction, compared to the conventional methods.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.383-391
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• 2008

The glycans linked to the insect cell-derived glycoproteins are known to differ from those expressed in mammalian cells, partly because of the low level or lack of glycosyltransferase activities. GnT II, GnT IV, GnT V, and ST3Gal IV, which play important roles in the synthesis of tetraantennarytype complex glycan structures in mammalian cells, were overexpressed in Trichoplusia ni cells by using a baculovirus expression vector. The glycosyltransferases, expressed as a fusion form with the IgG-binding domain, were secreted into the culture media and purified using IgG sepharose resin. The enzyme assay, performed using a pyridylaminated-sugar chain as an acceptor, indicated that the purified glycosyltransferases retained their enzyme activities. Human erythropoietin expressed in T. ni cells (rhEPO) was subjected to in vitro glycosylation by using recombinant glycosyltransferases and was converted into complex-type glycan with terminal sialic acid. The presence of Nacetylglucosamine, galactose, and sialic acid on the rhEPO moiety was detected by a lectin blot analysis, and the addition of galactose and sialic acid to rhEPO was confirmed by autoradiography using $UDP-^{14}C-Gal\;and\;CMP-^{14}C-Sia$ as donors. The in vitro glycosylated rhEPO was injected into mice, and the number of reticulocytes among the ed blood cells was counted using FACS. A significant increase in the number of reticulocytes was not observed in the mice injected with in vitro glycosylated rhEPO as compared with those injected with rhEPO.