• Journal of Advanced Marine Engineering and Technology
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• v.35 no.8
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• pp.1117-1123
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• 2011

Ungrounded power systems are adopted onboard vessels which enable more stabilized power supply even in case of electric leakage to hull. If earthing faults happen at these systems, they make grounding impedances of power lines unbalanced each other on the three phases, resulting in high voltages to hull which can bring more possibilities of electric shocks and electric fires. This study focuses on how to configure a calculation module for transferring a grounded condition by lowered insulation resistance into a vector diagram of the voltages to hull. By using the module, the loci of neutral points were acquired to analyze how voltages to hull are affected by earthing faults and the distributed capacitances between power lines and hull. The suggested module was simulated and compared to the measured values from a test power system in good results.

• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.161-166
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• 1998

To study the usefulness of Se301 cells, which is originated from Spodoptera exigua and has susceptibility to the Autographa californica NPV (AcNPV), as a host for the AcNPV-based expression vector system, we compared the characteristics of AcNPV in Se301 and Sf-21 cells. The symptom by viral infection was similar in both of cells, but the ratio of polyhedra released from the cell was higher in Se301 cells than in Sf-21 cells. The overall PIB productivity of AcNPV was similar in both cells but the size of polyhedra was larger in Se301 cells. While the polyhedrin expression efficiency was about 2.4 times higher in Se301 cells than in Sf-21 cells, the viral growth was higher in Sf-21 cells. These results suggested that Se301 cell is very useful in the AcNPV-based expression system as a host.

• Korean Journal of Veterinary Research
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• v.53 no.4
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• pp.217-224
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• 2013

Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-$S_{0.7}$) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-$S_{0.7}$ gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-$S_{0.7}$ protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-$S_{0.7}$ antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.1-6
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• 2011

Inefficiency of in vivo gene transfer using currently available vectors reflects a major hurdle in cancer gene therapy. Both viral and non-viral approaches have been described to improve gene transfer efficiency but suffer from a number of limitations. Here we tested an adenovirus carrying the small peptide ligand derived from heregulin${\beta}$ EGF-like domain onto fiber, the adenoviral capsid protein, to deliver transgene to ovarian cancer cells which overexpress ErbB, the cognate receptors for heregulin. The attachement of 53 amino acids to fiber didn't affect on the fiber's trimer structure that is critical for the viral entry to cells. The fiber-modified adenovirus can mediate entry and expression of a ${\beta}$-galactosidase into cancer cells in an increased efficiency compared the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, an ovarian cancer cell line. Such transduction systems hold promise for delivering genes to ErbB receptor overexpressing cancer cells, and could be used for future cancer gene therapy.

• BMB Reports
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• v.34 no.2
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• pp.176-181
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• 2001

Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatibility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDRI gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, thus virus will be used to infect hematopoietic stem cells to co-express MDRl and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.5
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• pp.998-1004
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• 2016

This paper investigates a new sensorless control appling a virtual flux oriented vector control without the line voltage sensor for the power factor correction of 3 phase PWM converter. The DC output voltage is controlled by applying the kalman filter algorithm for the virtual flux estimation and the synchronous phase is obtained by using the estimated virtual flux equation based on kalman filter. This method is used to reduce the calculation time of the system and obtain a stable control that the input current including the harmonics and the noise is improved. The proposed system implement PFC algorithm in the variable region of DC output voltage. It can obtain the unity power factor, and can precisely control the DC output voltage in the load variation and in the variable voltage range. The performance of the proposed algorithm is verified through simulation and experiment.

• The Transactions of the Korea Information Processing Society
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• v.5 no.9
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• pp.2294-2301
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• 1998

The aim of this paper is to extract features from each news scenes for example, symbol icon which can be distinct each broadcasting corp, icon and caption which are has feature and important information for the scene in respectively, In this paper, we propose extraction methods of caption that has important prohlem of news videos and it can be classified in three steps, First of al!, we converted that input images from video frame to YIQ color vector in first stage. And then, we divide input image into regions in clear hy using equalized color histogram of input image, In last, we extracts caption using edge histogram based on vertical and horizontal line, We also propose the method which can extract news icon in selected key frames by the difference of inter-histogram and can divide each scene by the extracted icon. In this paper, we used comparison method of edge histogram instead of complex methcxls based on color histogram or wavelet or moving objects, so we shorten computation through using simpler algorithm. and we shown good result of feature's extraction.

• Korean Journal of Poultry Science
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• v.26 no.2
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• pp.119-129
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• 1999

In recent years, numerous researches have been carried out in author's laboratory to develop several kinds of methods for producing transgened chicken, leaving a lot of new findings. Some of them are very useful to search for new approaches necessary to improve the efficiency of hatchability and the survival rate of developing trasgened embryos. The results obtained hitherto might be summarized as follows: (1) foreign gene(Lac Z/ Miw Z) introduced into blastodermal cells of developing embryos was successfully transferred to embryos, leading to the production of primordial germ cells(PGCs) carrying foreign DNA. However, hatched hickens failed to show the incorporation of introduced gene into the gonads. (2) When foreign gene was introduced into germinal crescent region (GCR), the gene was also efficiently incorporated into germ cells, resulting in the production of transgened chickens(offspring) which produced fruther offspring having foreign gene in the gonads. In this case, 2nd and 3rd generations of chickens were obtained through the reproduction of transgened birds. (3) In another way, the gene was injected into blood vessels of developing embryos at stage 13∼15, creating PGCs having foreign gene, and produced some transgened chickens. In this work, the PGCs were transfered between embryos, resulting in the production of transgenic chickens. (4) in these experiments, PGCs were effectively employed for producing transgenic birds, developing some kinds of chimeric chickens from homo- or hetero-sexual transfer of the PGCs from embryos. This means that the gonads from donor PGCs developed in some degree to the stage of hatching. However, these gonads showed slightly abnormal tissues similar to ovotestis like organs through histological examination. (5) Avian Leukosis Virus(ALV) induced B cell line(DT40) successfully carried foreign genes into chicken embryos, suggesting the possibility of the cells as a vector in this field of study in the future. (6) Inter-embryonic transfer of the PGCs also gave us some.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.9
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• pp.884-890
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• 2008

A new guidance law to reach circular target area with grazing angle constraint is proposed as one of midcourse guidance laws of unmanned air vehicles. The purpose of the law is to control the grazing angle between the velocity vector of the vehicle and the line of sight to the aiming point, the center of the circular target area, when the vehicle passes any point on the circle. The optimal solution is derived based on the optimal control theory minimizing a range weighted control energy subject to the nonlinear dynamic equations of the vehicle approaching to the circular target area with grazing angle constraint. The major properties including a convergence of the solution are examined and the performance of the law applied to some typical scenarios is shown by the numerical simulation.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.44-44
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• 2003

In previous reports, pVPSV.IGR2.1 transgenic mouse were described that brain tumor and lymphoma by reason of Vasopressin-SV40 T antigen. In this study, we produced pVPSV.IGR3.6 transgenic mouse that used pVPSV.IGR3.6 vector. Expression of transgene was vary different in transgenic mouse. We obtained 6 transgenic mouse line, moreover they had died at the age of 2~6 weeks without transmitting the transgene to their offspring, and had tumorigenesis on same location with pVPSV.IGR2.1 transgenic mouse. Only a founder mouse was investigated for expression of fusion gene. Here we extended this transgenic approach to the study of tumor progression. From the mouse, we confirmed brain tumor cell, after then cultured for investigate characterization. In this report, we demonstrate that reduction of survival rate in transgenic mouse fused vasopressin gene length, acquisition of brain tumor cell, composition with astrocyte cells and neuronal cells. Finally, cells had no change with increase of passage.