• Microbiology and Biotechnology Letters
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• v.49 no.1
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• pp.75-87
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• 2021

Type I Interferons (IFNα) are known for their role as biological anticancer agents owing to their cell-apoptosis inducing properties. Development of an appropriate, cost-effective host expression system is crucial for meeting the increasing demand for proteins. Therefore, this study aims to develop codon-optimized IFNα-2b in L. lactis NZ3900. These cells express extracellular protein using the NICE system and Usp₄₅ signal peptide. To validate the mature form of the expressed protein, the recombinant IFNα-2b was screened in a human colorectal cancer cell line using the cytotoxicity assay. The IFNα-2b was successfully cloned into the pNZ8148 vector, thereby generating recombinant L. lactis pNZ8148-SP_Usp45-IFNα-2b. The computational analysis of codon-optimized IFNα-2b revealed no mutation and amino acid changes; additionally, the codon-optimized IFNα-2b showed 100% similarity with native human IFNα-2b, in the BLAST analysis. The partial size exclusion chromatography (SEC) of extracellular protein yielded a 19 kDa protein, which was further confirmed by its positive binding to anti-IFNα-2b in the western blot analysis. The crude protein and SEC-purified partial fraction showed IC₅₀ values of 33.22 ㎍/ml and 127.2 ㎍/ml, respectively, which indicated better activity than the metabolites of L. lactis NZ3900 (231.8 ㎍/ml). These values were also comparable with those of the regular anticancer drug tamoxifen (105.5 ㎍/ml). These results demonstrated L. lactis as a promising host system that functions by utilizing the pNZ8148 NICE system. Meanwhile, codon-optimized usage of the inserted gene increased the optimal protein expression levels, which could be beneficial for its large-scale production. Taken together, the recombinant L. lactis IFNα-2b is a potential alternative treatment for colorectal cancer. Furthermore, its activity was analyzed in the WiDr cell line, to assess its colorectal anticancer activities in vivo.

• Composites Research
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• v.19 no.5
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• pp.28-33
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• 2006

In this paper, we have studied the complex permittivities and their influence on the design of microwave absorbers of E-glass fabric/epoxy composite laminates containing three different types of carbon-based nano conductive fillers such as carbon black (CB), carbon nano fiber (CNF) and multi-wall nano tube (MWNT). The measurements were performed fur permittivities at the frequency band of 0.5 GHz$\sim$18.0 GHz using a vector network analyzer with a 7 mm coaxial air line. The experimental results show that the complex permittivities of the composites depend strongly on the natures and concentrations of the conductive fillers. The real and imaginary parts of the complex permittivities of the composites were proportional to the filler concentrations. But, depending on the types of fillers and frequency band, the increasing rates of the real and imaginary parts with respect to the filler concentrations were all different. These different rates can have an effect on the thickness in designing the single layer microwave absorbers. The effect of the different rates at 10 GHz was examined by using Cole-Cole plot; the plot is composed of a single layer absorber solution line and measured permittivities from these three types of composites. Single layer absorbers of 3 different thicknesses using carbon nano materials were fabricated and the -10 dB band of absorbing performances were all about 3 GHz.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.21 no.1
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• pp.45-51
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• 2003

Stereo bird's-eyes-view was prepared for 3-dimensional view of various forms of Jecheon city, and 3-dimensional simulation was applied to it so as to show it in moving pictures in spatial. In manufacturing stereo bird's-eyes-view, perspective technology was used in image-making technology, and the basic material images are prepared as fellows: used EOC Images from Arirang-1 satellite, created DEM whose error was optionally geometric corrected after drawn from the contour line of the map on a scale of l/5,000 manufactured by national geography institute as a national standard map, and classified road lines which were manufactured as a road layer vector file of a map on a scale of l/l,000 and then overlay it over the three dimensional image of target area. Especially for the connectivity with address system to be used in new address, an arterial road map on a scale of l/l,000 that had been manufactured to grant new address was used in maximum in road network structure data of city area in this study.

• The Journal of the Korea institute of electronic communication sciences
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• v.15 no.1
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• pp.15-20
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• 2020

In this paper, delay diffusion for exhibition hall and conference room was measured and analyzed in the indoor environment of the building. Scenarios for the indoor environment of the two buildings were written. also, the system configuration and measurement methods were conducted under the same conditions. The measurement conditions were set to 3 GHz and 6 GHz of center frequencies and analysis band selected 2 GHz. The measurement system consisted of vector network analyzer, power amplifier, omni-directional transmit and receive antenna, and transmission line. According to the indoor environment scenario of the two buildings, the location of the receiving antenna was divided into three zones based on the location of the transmitting antenna and this was measured at 1 m intervals according to 18 locations. The power delay profiles, RMS delay spread, and K-factor results of two buildings were compared and analyzed.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.3
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• pp.61-69
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• 2010

This paper presents an efficient object selection algorithm by analyzing and detecting of human activity. Generally, when people point any something, they will put a face on the target direction. Therefore, the direction of the face and fingers and was ordered to be connected to a straight line. At first, in order to detect the moving objects from the input frames, we extract the interesting objects in real time using background subtraction. And the judgment of movement is determined by Principal Component Analysis and a designated time period. When user is motionless, we estimate the user's indication by estimation in relation to vector from the head to the hand. Through experiments using the multiple views, we confirm that the proposed algorithm can estimate the movement and indication of user more efficiently.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4435-4439
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• 2012

Purpose: This study aimed to explore the role of the Twist gene in the epithelial-mesenchymal transition of ovarian cancer. Methods: An RNA interference plasmid expressing a small interfering RNA (siRNA)-targeting Twist (Twist siRNA vector) was designed, constructed, and transfected into the human ovarian cancer cell line A2780. Transfection efficiency was assessed under a fluorescence microscope. Changes in the expression of Twist mRNA in A2780 after transfection with the pGenesil Twist shRNA plasmid were analyzed through RT-PCR. MTT assays and adhesion experiments were applied to determine changes in proliferation and adhesion ability of A2870 after transfection with the Twist shRNA plasmid. Changes in the expression of the E-cadherin and N-cadherin proteins in A2780 after transfection with the Twist shRNA plasmid were analyzed using Western blotting. Result: The restructuring plasmid pGenesil-Twist shRNA was constructed successfully. After 48 h of culture, 80% of the cells expressed high-intensity GFP fluorescence and stability. The expression of Twist decreased significantly after the transfection of the Twist shRNA plasmid (P<0.05). Proliferation of the transfected Twist shRNA cells showed no difference with that of the A2780-nontransfection or A2780-si-control groups (P>0.05) but the adhesion ability of A2780 decreased dramatically (P<0.05). Expression of the E-cadherin protein increased, whereas that of the N-cadherin protein decreased compared with that in the A2780-nontransfection or A2780-si-control groups (P<0.05). Conclusion: Twist is essential for epithelial-mesenchymal transition, invasion, and metastasis of ovarian cancer.

• The Journal of the Acoustical Society of Korea
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• v.24 no.7
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• pp.423-430
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• 2005

In this paper. a microphone array beamforming algorithm that reduces the signal distortion caused by reverberation and near-field effect in car environment is proposed. When reverberation or near-field effect is present, an optimum beamformer should be constructed with a steering vector consisting of transfer functions between source and microphones, but it is generally difficult to estimate transfer functions on-line without knowledge of the source signal. Instead, a sub-optimal beamforming algorithm that reduces signal distortion is proposed. It is constructed with steering vectors consisting of relative transfer functions between reference sensor and other sensors. In order to evaluate the performance of the proposed algorithm. we had recorded noisy speech database in a car, and performed speech recognition experiments with HMM Toolkit (HTK) released by Cambridge University. The recognition rate of the proposed algorithm was 15 percents higher than that of the conventional far-field beamformers in best case.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.11
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• pp.1574-1579
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• 2006

This study was performed to determine the developmental potentials of nuclear transfer (NT) embryos using life-span extended cells transfected with a foreign gene as donor cells. A life-span extended bovine embryonic fibroblast cell line was transfected with an expression vector in which the human type II collagen (BOMAR) and ear fibroblasts were used as a donor cell. Cytogenetic analysis was performed to analyze the chromosomal abnormality of donor cells. The fusion rate of 1.8 kV/cm for $15{\mu}sec$ given twice was significantly higher than that of other groups (p<0.05) and the embryos lysed were significantly higher after 1.8 kV/cm for $20{\mu}sec$ given once compared to other groups (p<0.01). The blastocyst development in the ear cell group was statistically significant compared to both BOMAR groups (p<0.01). Both BOMAR groups cultured more than 40 passages (>40 passages) had a lower number of chromosomes; however, fresh granulosa cell (GC) and BOMAR groups cultured less than 20 passages had normal chromosome numbers. Both >40 passages BOMAR groups had numerous obscure debris in metaphase spreads. The transfected foreign gene was expressed in all BOMAR groups, but not in the GC group. Based on these results, the lower developmental potential of NT embryos using life-span extended donor cells transfected with a foreign gene might be a cause of chromosomal abnormality in donor cells.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.4
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• pp.1730-1747
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• 2018

Multi-view video plus depth (MVD) is a mainstream format of 3D scene representation in free viewpoint video systems. The advanced 3D extension of the high efficiency video coding (3D-HEVC) standard introduces new prediction tools to improve the coding performance of depth video. However, the depth video in 3D-HEVC is time consuming. To reduce the complexity of the depth video inter coding, we propose a fast coding unit (CU) size and mode decision algorithm. First, an off-line trained Bayesian model is built which the feature vector contains the depth levels of the corresponding spatial, temporal, and inter-component (texture-depth) neighboring largest CUs (LCUs). Then, the model is used to predict the depth level of the current LCU, and terminate the CU recursive splitting process. Finally, the CU mode search process is early terminated by making use of the mode correlation of spatial, inter-component (texture-depth), and inter-view neighboring CUs. Compared to the 3D-HEVC reference software HTM-10.0, the proposed algorithm reduces the encoding time of depth video and the total encoding time by 65.03% and 41.04% on average, respectively, with negligible quality degradation of the synthesized virtual view.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.105-105
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• 2003

In previous reports, pVPSV.IGR2.1 transgenic mouse were described that brain tumor and lymphoma by reason of Vasopressin-SV40 T antigen. In this study, we produced pVPSV.IGR3.6 transgenic mouse that used pVPSV.IGR3.6 vector. Expression of transgene was vary different in transgenic mouse. We obtained 6 transgenic mouse line, moreover they had died at the age of 2-6 weeks without transmitting the transgene to their offspring, and had tumorigenesis on same location with pVPSV.IGR2.1 transgenic mouse. Only a founder mouse was investigated for expression of fusion gene. Here we extended this transgenic approach to the study of tumor progression. From the mouse, we confirmed brain tumor cell, after then cultured for investigate characterization. In this report, we demonstrate that reduction of survival rate in transgenic mouse fused vasopressin gene length, acquisition of brain tumor cell, composition with astrocyte cells and neuronal cells. Finally, cells had no change with increase of passage.