• Title/Summary/Keyword: Various reducing conditions

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Antioxidant activities of Erythrina stricta Roxb.using various in vitro and ex vivo models

  • AsokKumar, K;UmaMaheswari, M;Sivashanmugam, AT;SubhadraDevi, V;Subhashini, N;Ravi, TK
    • Advances in Traditional Medicine
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    • v.8 no.3
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    • pp.266-278
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    • 2008
  • Erythrina stricta, a deciduous tree widely used traditionally in indigenous system of medicine for various ailments such as rheumatism, fever, leprosy, epilepsy etc. The leaves of Erythrina stricta was extracted with ethanol (70%) and used for the evaluation of various in vitro antioxidant assays which includes H - donor activity, nitric oxide scavenging, superoxide anion scavenging, reducing ability, hydroxyl radical, hydrogen peroxide scavenging, total phenolic content, total flavonoid content, total antioxidant activity by thiocyanate and phosphomolybdenum method, metal chelating, $\beta$-carotene bleaching, total peroxy radical assays. The pro-oxidant activity was measured using bleomycin-dependent DNA damage. Ex vivo models like lipid peroxidation and erythrocyte haemolysis were also used to study the antioxidant property of the extract. The various antioxidant activities were compared with suitable standard antioxidants such as ascorbic acid, butylated hydroxyl toluene, $\alpha$-tocopherol, curcumin, quercetin and Trolox. The generation of free radicals viz. $O_2^{{\cdot}-}$, $OH^{\cdot}$, $H_2O_2$, $NO^{\cdot}$ and peroxyl radicals were effectively scavenged by the ethanolic extract of Erythrina stricta. In all the methods, the extract offered strong antioxidant activity in a concentration dependent manner. The total phenolic content, flavonoid content and total antioxidant activity in Erythrina stricta were determined as microgram (g) pyrocatechol, quercetin and $\alpha$-tocopherol equivalent/mg respectively. The extract did not exhibit any prooxidant activity when compared with ascorbic acid. The results obtained in the present study clearly indicates that Erythrina stricta scavenges free radicals and reduces lipid peroxidation, ameliorating the damage imposed by oxidative stress in different disease conditions and serve as a potential source of natural antioxidant.

A Study on the pH Reduction of Cement Concrete with Various Mixing Conditions (시멘트 콘크리트의 배합조건에 따른 pH 저감에 관한 연구)

  • Jo, Young-Kug
    • Journal of the Korea Institute of Building Construction
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    • v.8 no.4
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    • pp.79-85
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    • 2008
  • The purpose of this study is to evaluate the mix design of pH reducing cement concrete which can be used for environment-friendly concrete. Cement pastes and concretes are prepared with water-binder ratios and various admixtures such as blast-furnace slag, fly ash and recycled cement, and tested for compressive strength and pH. pH is measured through pore solution expressed from hydrated cement paste by special apparatus. From the test results, regardless of water-binder ratio, The pH of expressed pore solution from hydrated cement paste which is made of ordinary portland cement with blast-furnace slag, fly ash is decreased with increasing of admixtures content, and compressive strength is also slightly improved. The compressive strength of cement paste made of recycled cement which is burnt at $1000^{\circ}C$, for 2 hours is considerably increased compared with that of none-burnt recycled cement due to restoration of hydraulic property, but pH is a little higher. Porous concrete with ordinary portland cement has high pH in the range of 12.22 to 12.59, however, that is reduced to the range of 8.95 to 10.39 by carbonation at the surface of porous concrete. The pH reduction of porous concrete is possible by various admixture addition, however their degrees are very slight. Therefore, to reduce the pH considerably, carbonation method of porous concrete is better in pH reduction methods for plant survival condition of pH of 9.0 or less. In this study, it is apparent that pH for the environment-friendly porous concrete products used in the construction field can be suppressed by this carbonation method and various admixtures addition.

Fog Generated Field Test for Luminance Criteria of Variable Speed-Limit Signs (가변속도형 표지 휘도기준 정립을 위한 안개재현 현장실험)

  • Kim, Yongseok;Lee, Sukki;Kim, Soullam
    • International Journal of Highway Engineering
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    • v.18 no.6
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    • pp.77-85
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    • 2016
  • OBJECTIVES : A fog generated field test was conducted to analyze the relationship between the luminance of variable speed-limit signs and the legibility distance under various fog density conditions. By using this study, appropriate luminance values can be selected depending on the density of fog. METHODS : An actual tunnel was selected as the area to conduct the test, as other places cannot maintain the fog condition because of rapid air current. Ninety-two subjects were recruited for this test, which took place during the course of three days. Visibility-distance detecting sensor was used to measure the visibility distance due to the fog density time, simultaneously with the evaluation of legibility distance by subjects. RESULTS : The test results show the relationship between luminance values and the legibility distance corresponding to the visibility distance due to fog. According to the technical test results, lower luminance value such as $7000cd/m^2$ corresponds to less legibility distance compared to higher values such as $20000cd/m^2$ or $40000cd/m^2$. However, the amount of difference between $20000cd/m^2$ and $40000cd/m^2$ is negligible in our test. CONCLUSIONS : The results of this study can be used to select appropriate luminance of valuable speed signs under fog conditions. Hence, drivers can expect to have more room to respond to adverse weather conditions, thereby reducing the risk of accidents.

Changes in Tasting Constituents (Tannin, Free Sugar, Total Nitrogen ) of Green Tea by Leaching Condition (침출조건에 따른 녹차의 성분(Tannin, Free Sugar, Total Nitrogen) 함량변화)

  • Lee, Mi-Gyeong;Lee, Sung-Woo;Kim, Sung-Soo;Lee, Sang-Hyo;Oh, Sang-Lyong
    • Journal of the Korean Society of Food Culture
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    • v.4 no.4
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    • pp.411-416
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    • 1989
  • Optimum leaching conditions of Korean green tea were determined by examining the relationship between sensory evaluation and tasting constituents of green tea infusion at various leaching conditions. Total nitrogen, total reducing sugar and tannin contents of green tea were $4.70{\sim}5.02%$, $16.5{\sim}18.0%$ and $10.3{\sim}12.7%$, respectively. They increased with the increased of leaching temperature and time. The sensory score was significantly correlated with the tannin contents of green tea in fusion in the range of $15{\sim}30\;mg/g$.(r=-0.9061). Optimum leaching conditions of green tea were $0.5{\sim}1.0$ min at $55^{\circ}C$, 0.5 min at $70{\sim}85^{\circ}C$ in high grade parched green tea, $1.0{\sim}2.0$ min at $55^{\circ}C$ and $0.5{\sim}1.0$ min at $70^{\circ}C$ in medium one.

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Effect of curing on alkalinity and strength of cement-mortar incorporating palm oil fuel ash

  • Payam Shafigh;Sumra Yousuf;Belal Alsubari;Zainah Ibrahim
    • Advances in concrete construction
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    • v.15 no.3
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    • pp.191-202
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    • 2023
  • Palm oil fuel ash (POFA) is a newly emerging pozzolanic material having high amount of silica content. Various forms of POFA were used in cement-based materials (CBMs) in replacement of cement in different dosages of low and high volume. Although, there are many researches on POFA to be used in concrete and mortar, however, this material was not practically used in the construction industry. Engineers and designers need to be confident to use any new developed materials by knowing all engineering properties at short and long terms. As durability concern, concrete pH value is one of the most important properties. Portland cement produces are alkaline initially, however, it may be reduced due to aging and its components. It is believed that by incorporation of supplementary cementitious materials in CBMs the pH value reduces due to utilization of Ca(OH)2 in pozzolanic reaction. This study is the first attempts to understand the pH value of mortars containing up to 30% POFA under different curing conditions and its changes with time. The results were also compared with the pH of ground granulated ballast furnace slag (GGBFS) and fly ash (FA) content mortars. In addition, the compressive strength of different mortars under different curing conditions were also studied. The results showed that the pH value of control mix (without cementitious materials) was more than all the blended cement mortars indifferent curing conditions at the same ages. However, there was a reducing trend in the pH value of all mortar mixes containing POFA.

Screening of Extraction Solvent Condition of Fermented Rhus verniciflua Stem Bark by Antioxidant Activities (항산화활성 비교를 통한 발효 칠피의 추출용매 조건 탐색)

  • Kim, Myeong-Ok;Kim, Ju-Sung;Sa, Yeo-Jin;Jeong, Hyun-Ju;Chun, Wan-Joo;Kwon, Yong-Soo;Kim, Tae-Young;Choi, Han-Seok;Yu, Chang-Yeon;Kim, Myong-Jo
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.4
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    • pp.217-223
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    • 2010
  • In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with $RC_{50}$ value of $10.5{\pm}1.4{\mu}g/m{\ell}$, reducing power value 1.04 Abs (concentration of 1 mg/$m{\ell}$), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 mg/$m{\ell}$). M80 extract showed the high content of total phenolic (319.7 mg GAE/$m{\ell}$ extract) and total flavonoid (111.6 mg QE/$m{\ell}$ extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging ($R^2$=0.911 and 0.912, each extract) and reducing power ($R^2$=0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity ($R^2$=0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.

Reduced Protein Denaturation in Thermotolerant Cells by Elevated Levels of HSP70 (열내성이 유도된 세포에서 HSP70 단백질 증가에 의한 단백질 변성 감소)

  • Han, Mi-Young;Park, Young-Mee
    • The Korean Journal of Pharmacology
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    • v.32 no.3
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    • pp.433-444
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    • 1996
  • We describe a novel approach to evaluate quantitatively the amounts of denatured proteins in cells upon heat exposure. A thiol compound, diamide [azodicarboxylic acid bis (dimethylamide)] causes protein cross-linking with exposed sulfyhydryl residues of denatured proteins. Since denatured proteins expose normally well-hidden sulfhydryl groups, these will be preferentially cross-linked by diamide. Thus diamide acts to 'trap' denatured proteins. We observed that protein aggregates (high molecular weight protein aggregates, HMA) appeared on SDS-polyacrylamide gels run under non-reducing conditions and that the amount of HMA can be quantified by scanning the gels using a gas flow counter. Heating cells followed by a fixed dose of diamide exposure resulted in HMA increases in a heat-dose dependent manner, demonstrating that the quantitation of HMA could serve as a measure of heat-denatured proteins. We compared thermotolerant and nontolerant cells and found decreased HMA in tolerant cells upon heat treatment. As an attempt to examine the kinetics of protein renaturation (or 'repair'), we measured the amounts of aggregates formed by the addition of diamide at various times after heat shock. Such experiments demonstrate an equally rapid disappearance of HMA in previously unheated and in thermotolerant cells. Levels of HMA in tolerant cells increased significantly after electroporation of HSP70 specific mAbs, suggesting an involvement of HSP70 in reducing HMA levels in thermotolerant cells upon heat exposure. Immunoprecipitation studies using anti-HSP70 antibody indicated an association of HSP70 with heat-denatured proteins. Our results suggest that heat induces protein denaturation, and that elevated level of HSP70 present in thermotolerant cells protects them by reducing the level of protein denaturation rather than by facilitating the 'repair' (or degradation) process.

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The Influences of Pulsed Electromagnetic Field Treatment Following Experimentally Induced Delayed-Onset Muscle Soreness in Biceps Brachii (펄스자기장이 위팔 두갈래근의 지연성 근육통에 미치는 영향)

  • Kang, Sun-Young;Park, Joo-Hee;Jeon, Hye-Seon;Lee, Hyun-Sook
    • Physical Therapy Korea
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    • v.20 no.2
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    • pp.11-19
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    • 2013
  • Delayed onset muscle soreness (DOMS) is a painful condition that arises from exercise-induced muscle damage after unaccustomed physical activities. Various therapeutic interventions have been applied to reduce the intensity and duration of DOMS-related symptoms. Recently, pulsed electromagnetic field (PEMF) intervention has been introduced as an alternative noninvasive treatment for DOMS. This randomized, double-blind, placebo-controlled experiment was conducted to examine the effects of PEMF therapy on DOMS in elbow flexors at 24, 48, and 72 hours after the experimental DOMS induction. Thirty healthy volunteers ($23{\pm}2.4$ yrs, $175{\pm}5.7$ cm, and $74{\pm}7.8$ kg) participated in this study. Each was randomly assigned to a PEMF or placebo group. On the first day, DOMS was induced in the elbow flexors by repeated isokinetic motions at low ($60^{\circ}/s$) and fast ($120^{\circ}/s$) speeds in all subjects. Thereafter, the PEMF group received 15-min daily treatment with a PEMF device. The placebo group received sham treatment of the same duration. Overall, PEMF application was more effective than the sham treatment in reducing the physiological symptoms associated with the DOMS including perceived soreness, median frequency, and electromechanical delay of the surface electromyography. In addition, median frequency and isokinetic peak torque of the PEMF group recovered to the pre-DOMS induction level earlier than the placebo group. In conclusion, this study suggests that PEMF can be applied as a new recovery strategy in reducing DOMS symptoms. Further experiments are required to examine the effect of the PEMF treatment on different types of exercise conditions and to determine the optimal treatment dosage and duration in a real clinical setting.

Effects of Process Variables on Preparation of Silver-Coated Copper Flakes Using Hydroquinone Reducing Agent (하이드로퀴논 환원제를 사용한 은코팅 구리 플레이크의 제조에서 공정 변수의 영향)

  • Chee, Sang-Soo;Lee, Jong-Hyun
    • Journal of the Microelectronics and Packaging Society
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    • v.24 no.3
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    • pp.57-62
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    • 2017
  • In the process for preparing Ag-coated Cu flakes by electroless silver plating using hydroquinone reducing agent, Ag coating qualities were compared by changing various process parameters such as type of pretreatment solution, plating temperature, pH of plating solution, type and injection rate of plating solution, and pulp density. Effective pretreatment solution for removing the oxide layer on a Cu flake was preferentially suggested. The conditions of low plating temperature, pH value of 4.34, slow injection rate of Ag plating solution, elimination of deionized water in the Ag plating solution, and high pulp density significantly suppressed the formation of separated tiny Ag particles, and thus the surface coverage of Ag coating on Cu flakes was enhanced.

Annual energy yield prediction of building added PV system depending on the installation angle and the location in Korea (건물적용 태양광발전시스템의 국내 지역에 따른 설치각도별 연간 전력생산량 예측에 관한 연구)

  • Kim, Dong Su;Shin, U Cheol;Yoon, Jong Ho
    • KIEAE Journal
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    • v.14 no.1
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    • pp.67-74
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    • 2014
  • There have distinctly been no the installation criteria and maintenance management of BIPV systems, although the BIPV market is consistently going on increasing. In addition, consideration of the BIPV generation quantity which has been installed at several diverse places is currently almost behind within region in Korea. Therefore, the main aim of this study is to evaluate the BIPV generation and to be base data of reducing rate depending on regional installation angles using PVpro which was verified by measured data. Various conditions were an angle of inclination and azimuth under six major cities: Seoul, Daejeon, Daegu, Busan, Gwangju, Jeju-si for the BIPV system generation analysis. As the results, Seoul showed the lowest BIPV generation: 1,054kWh/kWp.year, and Jeju-si have 5percent more generation: 1,108.0kWh/kWp.year than Seoul on horizontal plane. Gwangju and Daejeon turned out to have similar generation of result, and Busan showed the highest generation: 1,193.5kWh/kWp.year, which was increased by over 13percent from Seoul on horizontal plane. Another result, decreasing rate of BIPV generation depending on regional included angle indicate that the best position was located on azimuth: $0^{\circ}$(The south side) following the horizontal position(an angle of inclination: $30^{\circ}$). And the direction on a south vertical position(azimuth: $0^{\circ}$, an angle of inclination: $90^{\circ}$) then turned out reducing rate about 40percent compared with the best one. Therefore, these results would be used to identify the installation angle of the BIPV module as an appropriate position.