• Journal of Microbiology
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• v.37 no.3
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• pp.180-184
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• 1999

hybridoma cells producing IgM anti-pneuococcal 6B polysaccharide antibodies were induced to switch to IgG-producing cells in vitro by treating with acridine orange. Treating 0.5 $\mu\textrm{g}$/ml of acridine orange for 24 hours generated maximal number of variant cells. The maximal isotype switch frequency was 3${\times}$10-5, which is about 30-fold higher than the frequency of spontaneous switching. Resulting IgG-producing variants were enriched by sib selection and ELISA spot assay. Two IgG3-producing variant cells were finally cloned by limiting dilution. The variant cells produced similar amounts of antibodies as their parental cells did. The two switched antibodies had similar reactivity to pneumococcal 6B polysaccharide. When compared to their parental IgM antibodies, the switched IgG3 than that of IgM antibody. The antibodies will be useful as essential tools for comparative study of the role of heavy chain isotypes in protection against Streptococcus pneumoniae.

• Korean Journal of Cognitive Science
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• v.3 no.1
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• pp.25-39
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• 1991

When an ending which begins with 'a/a'combines to a stem,'a/a'can be deleted.Especially when ot combines to an h-irregular verb,it is represented as a variant like '-a-','-e-','-ia-',or'-ie-'.In order to analyze the variants of 'a/a',we suggest the format of a grammatical morpheme dictionary which is represented as a binary tree and several procedures which process the variants so that the unexpected errors can be removed which occur frequently when we analyze Korean worl phrase.

• Journal of the Korean Operations Research and Management Science Society
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• v.32 no.2
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• pp.99-107
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• 2007

We consider (worst-case) robust optimization versions of the Economic Order Quantity (EOQ) model with decreasing cost functions. Two variants of the EOQ model are discussed, in which the purchasing costs are decreasing power functions in either the order quantity or demand rate. We develop the corresponding worst-case robust optimization models of the two variants, where the parameters in the purchasing cost function of each model are uncertain but known to lie in an ellipsoid. For the robust EOQ model with the purchasing cost being a decreasing function of the demand rate, we derive the analytical optimal solution. For the robust EOQ model with the purchasing cost being a decreasing function of the order quantity, we prove that it is a convex optimization problem, and thus lends itself to efficient numerical algorithms.

• BMB Reports
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• v.48 no.2
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• pp.61-67
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• 2015

Norovirus is a major cause of viral gastroenteritis and a common cause of foodborne and waterborne outbreaks. Norovirus outbreaks are responsible for economic losses, most notably to the public health and food industry field. Norovirus has characteristics such as low infectious dose, prolonged shedding period, strong stability, great diversity, and frequent genome mutations. Besides these characteristics, they are known for rapid and extensive spread in closed settings such as hospitals, hotels, and schools. Norovirus is well known as a major agent of food-poisoning in diverse settings in South Korea. For these reasons, nationwide surveillance for norovirus is active in both clinical and environmental settings in South Korea. Recent studies have reported the emergence of variants and novel recombinants of norovirus. In this review, we summarized studies on the molecular epidemiology and nationwide surveillance of norovirus in South Korea. This review will provide information for vaccine development and prediction of new emerging variants of norovirus in South Korea.

• Proceedings of the KSRS Conference
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• v.2
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• pp.615-618
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• 2006

By introducing Web Services, distributed GIS services from different vendors can be dynamically integrated into a GIS application using the interoperable standard SOAP protocol. However, it is debatable whether SOAP can really meet the performance requirements of GIS. This paper presents an experimental evaluation of the performance of different SOAP variants: standard SOAP, SwA/MIME, and SOAP/MTOM. The objective of this paper is to demonstrate that SOAP performance in communicating large volumes of GIS data could be effectively improved by recent standards. Moreover, SOAP/MTOM is identified to be the fastest and the most efficient messaging protocol.

• Journal of the Korean Academy of Esthetic Dentistry
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• v.12 no.2
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• pp.31-38
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• 2003

Amelogenesis imperfecta patients suffered common clinical problems of poor esthetics, teeth sensitivity, and loss of occlusal vertical dimension. Amelogenesis imperfecta is a group of inherited disorders primarily affecting dental enamel. Variants of amelogenesis imperfecta generally classified hypoplastic, hypocalcified, or hypomaturation types based on the primary enamel defects. The mildest problems were found in the pitted hypoplastic type whereas the most severe problems were encountered in the hypocalcified type amelogenesis imperfecta. Management stragies include composite resin veneer and jacket crowns for anterior teeth as well as steel crowns for posterior teeth. Knowledge of the clinical features and dental complications of each variants if amelogenesis impecta helps in the diagnosis of the condition and allows institution of early preventive measures. The objective of this paper is to provide a review of the current concepts of the wide spectrum of etiological factors involved in the pathogenesis of this significance clinical entity in the primary dentition.

• Phonetics and Speech Sciences
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• v.7 no.1
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• pp.133-138
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• 2015

The purpose of this study is to provide deeper understanding of how it is possible for people to understand PC communication language they have never seen or heard before without any problem. In order to answer this question, we focused on the vowel variation through which new variants are created (for PC communication), and hypothesized that there is a phonetic constraint which requires the vowel of the variant to be phonetically similar (to the maximum) to the vowel of the original word. Through the corpus analysis of the dictionary of PC communication language, we show that our hypothesis is justified by the fact that most of the variants we collected from the dictionary, that is, 90% of them, conformed to the phonetic constraint we postulated.

• 대한관절경학회:학술대회논문집
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• 2009.10a
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• pp.183-187
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• 2009

Labral lesions and anatomic variants have been studied by several authors in the last decade. Buford complex and sublabral recess and foramen are most common variants. Their recognition is important in order to distinguish them from superior and anterior labral tear. We report rare normal variant of biceps anchor and superior labrum that was observed during arthroscopic surgery for SLAP lesion.

• Korean Journal of Computational Design and Engineering
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• v.5 no.3
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• pp.224-231
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• 2000

In order to satisfy customers'various needs in the market, manufacturing companies tend to add similar variants to an existing product model. This variety causes immense growth of product configuration and makes data management uncontrollable. In order to resolve this problem, we propose a method to efficiently represent the variants in a single schema of product structure and configuration and manage the product configuration. First of all, the product structure which adopts the concept of features and options is suggested. Second, the method to represent option specifications which restricts option configurations is proposed. Finally, the prototype module, which inspects if a product configuration violates the option specifications, is introduced.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.212-218
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• 2006

Three out of fourteen Microcystis-dominant cyanobacterial blooms in Central India were found to be toxic to mice ($LD_{50}$ ranging from 35-450 mg bloom dry mass/kg body weight). The liver architecture of the treated mice showed characteristic symptoms of hepatotoxicity relative to the untreated controls, with increased enzyme activities of serum lactate dehydrogenase (LDH), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), and serum glutamate pyruvate transaminase (SGPT). RP-HPLC revealed the presence of microcystin-LR, microcystin-RR, and desmethyl microcystin-RR in the given region to maximum amounts of 390, 1,030, and $860{\mu}g/g$ bloom dry weight, respectively, corresponding to a maximum of 2.8 mg/l microcystin-LR in the lake water. Further confirmation of the microcystin variants was conducted using a MALDI-TOF MS analysis.