• Journal of Pharmaceutical Investigation
• /
• v.37 no.5
• /
• pp.315-321
• /
• 2007

The purpose of the present study was to evaluate the bioequivalence of two lercanidipine hydrochloride tablets, Zanidip tablet (LG Life Sciences Ltd., Korea, reference drug) and Samchundang Lercanidipine tablet 10 mg (Sam Chun Dang Pharm. Co. Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (amlodipine maleate) to human serum, serum samples were extracted using hexan-isoamyl alcohol (100:1, v/v). Compounds were analyzed by liquid chromatography/tandem mass spectrometry. This method showed linear response over the concentration range of 0.05-20 ng/mL with correlation coefficient of 0.9999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ng/mL which was sensitive enough for pharmacokinetic studies. Thirty healthy male Korean volunteers received each medicine at the lercanidipine hydrochloride dose of 20 mg in a $2\;{\times}\;2$ crossover study. There was a one-week washout period between the doses. Serum concentrations of lercanidipine were monitored by an LC/MS/MS fer over a period of 24 hr after the administration. $AUC_t$ (the area under the serum concentration-time curve from time 0 to 24 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (the maximum serum drug concentration) and $T_{max}$ (the time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters, indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Samchundang Lercanidipine/Zanidip were log 0.9505-log 1.2258 and log 0.9987-log 1.2013, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Samchundang Lercanidipine tablet 10 mg and Zanidip tablet are bioequivalent.

• Journal of Pharmacopuncture
• /
• v.17 no.3
• /
• pp.25-30
• /
• 2014

Objectives: This study was performed to evaluate the single-dose intravenous toxicity of Guseonwangdo-go glucose 20% pharmacopuncture. Methods: Forty Sprague-Dawley rats were divided into four groups of five males and five females per group: an intravenous (IV) injection of 1.0 mL of normal saline solution per animal was administered to group 1 (G1, control group); an IV injections of 0.1, 0.5, and 1.0 mL of Guseonwangdo-go glucose pharmacopuncture per animal were administered to experimental groups 2, 3, and 4 (G2, G3, and G4), respectively. General symptoms, body weights, hematological and biochemical test results, and necropsy histopathological observation were recorded in all groups. In the statistical analyses, significance was determined by using the one-way analysis of variance (ANOVA). The significance level was 0.05 in all comparisons. Results: For 14 days, no deaths or abnormalities were observed in any of the 4 groups. The body weights of all groups continuously increased during the observation period. In the hematological test, the WBC count was significantly increased in female rats of G4 compared to the control group, but this difference was considered not to be statistically meaningful. No significant biochemical changes were observed. On necropsy, crust formation was observed in one rat of the control group, and granulation tissues were observed around the injection site in one rat of G4; these changes were concluded to have been caused by injection of the needle into a vein. Conclusion: The findings suggest that the lethal dose of Guseonwangdo-go glucose pharmacopuncture is more than 1.0 mL per animal in both male and female rats. Thus, we can conclude that Guseonwangdo-go glucose pharmacopuncture injection is relatively safe to use in acute toxicity tests. Further studies are needed to establish more detailed evidences of its toxicity.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.31 no.1
• /
• pp.60-69
• /
• 2005

Orthopedic rapid maxillary expansion(RME) has been a common treatment modality used to widen narrow maxillae in young children. However, since more skeletally matured adolescents or adults has closed midpalatal suture, the result of RME was undesirable because of dental tipping with little or no basal skeletal movement and resulted to many other complications. After such treatment, complications often occurred such as alveolar bending, compression of periodontal ligament, extrusion, buccal tipping, and severe relapse. Thus, surgically assisted rapid maxillary expansion(SA-RME) is required, especially for patients over 14 years old, to skeletally release maxillary expansion. We used two methods of maxillary expansion surgery. Surgically assisted rapid maxillary expansion(SA-RME) & surgically assisted posterior segmental expansion(SA-PSE) were used for narrow maxilla. The study was divided into two groups(SA-RME group and SA-PSE group). SA-RME group was consisted of 2 males and 4 females, and the ages of materials ranged from 15 years to 25 years with a mean of 20.2 years. SA-PSE group was consisted of 1 male and 5 females, and the ages ranged from 13 years to 23 years with a mean of 18.7 years. Dental study models were fabricated before starting the expansion and immediately after the expansion was completed. It was fabricated again 1 month later, 3 months later when the expansion device was removed, and 6 months later after the expansion was completed. A repeated measures analysis of variance(ANOVA) test was applied to assess changes between each groups over time. The amount of expansion and the amount of tipping movement each in both groups were compared by using paired t-test and it was also compared between each subjects within the group by using independent t-test. Both SA-RME and SA-PSE group showed stable results, but SA-PSE group showed statical significance in tipping movement of second premolar. We compared 6 patients who recieved SA-RME with 6 patients who received SA-PSE, and appraised the clinical usefulness.

• Animal Bioscience
• /
• v.35 no.7
• /
• pp.1010-1020
• /
• 2022

Objective: The experiment was conducted to evaluate the effects of maternal undernutrition during late pregnancy on the expressions of genes involved in growth and development in ovine fetal perirenal brown adipose tissue (BAT). Methods: Eighteen ewes with singleton fetuses were allocated to three groups at day 90 of pregnancy: restricted group 1 (RG1, 0.33 MJ metabolisable energy [ME]/kg body weight [BW]^0.75/d, n = 6), restricted group 2 (RG2, 0.18 MJ ME/kg BW^0.75/d, n = 6), and a control group (CG, ad libitum, 0.67 MJ ME/kg BW^0.75/d, n = 6). The fetuses were removed at day 140 of pregnancy. All data were analyzed by using the analysis of variance procedure. Results: The perirenal fat weight (p = 0.0077) and perirenal fat growth rate (p = 0.0074) were reduced in RG2 compared to CG. In fetal perirenal BAT, the protein level of uncoupling protein 1 (UCP1) (p = 0.0001) was lower in RG1 and RG2 compared with CG and UCP1 mRNA expression (p = 0.0265) was decreased in RG2. The protein level of myogenic factor 5 (Myf5) was also decreased in RG2 (p = 0.0001). In addition, mRNA expressions of CyclinA (p = 0.0109), CyclinB (p = 0.0019), CyclinD (p = 0.0015), cyclin-dependent kinase 1 (CDK1) (p = 0.0001), E2F transcription factor 1 (E2F1) (p = 0.0323), E2F4 (p = 0.0101), and E2F5 (p = 0.0018) were lower in RG1 and RG2. There were decreased protein expression of peroxisome proliferator-activated receptor-γ (PPARγ) (p = 0.0043) and mRNA expression of CCAAT/enhancer-binding protein-α (C/EBPα) (p = 0.0307) in RG2 and decreased PPARγ mRNA expression (p = 0.0008) and C/EBPα protein expression (p = 0.0015) in both RG2 and RG1. Furthermore, mRNA expression of bone morphogenetic protein 4 (BMP4) (p = 0.0083) and BMP7 (p = 0.0330) decreased in RG2 and peroxisome proliferator-activated receptor co-activator-1α (PGC-1α) reduced in RG2 and RG1. Conclusion: Our observations support that repression of regulatory factors promoting differentiation and development results in the inhibition of BAT maturation in fetal perirenal fat during late pregnancy with maternal undernutrition.

• Journal of Environmental Health Sciences
• /
• v.50 no.3
• /
• pp.181-190
• /
• 2024

Background: Air pollutants have been reported to have harmful effects on human health. Busan is a vulnerable area in terms of air quality due to the installation of various industrial complexes, particularly the port industry. However there is limited research data on the ambient air quality of residential areas near ports and industrial complexes. Objectives: This study aimed to determine the quarterly levels of air pollutants near industrial complexes and ports and to identify trends and characteristics of air pollutant exceedances. Methods: Air measurements were conducted quarterly. The measured air pollutants included O₃, SO₂, CO, NO₂, PM₁₀, and PM_2.5. PM₁₀ and PM_2.5 were measured using BAM-1020 equipment, while O₃, SO₂, CO, and NO₂ were measured using AP-370 Series equipment. The quarterly concentration levels of air pollutants were determined, and the influence of precipitation and commuting hours on fine particulate matter was examined. Analysis of variance (ANOVA) was conducted to determine if there was significance between the concentrations of fine particulate matter during commuting hours and non-commuting hours. Results: The concentrations of air pollutants were generally higher in the first and second quarters. Furthermore, the concentrations of PM₁₀ and PM_2.5 tended to decrease continuously following consecutive rainfall, with concentrations at the end of rainfall periods lower than those observed at the beginning. The frequency of exceeding average concentrations of PM₁₀ and PM_2.5 was higher on weekdays. Moreover, the average concentrations of PM₁₀ and PM_2.5 during weekday commuting hours were higher compared to non-commuting hours. Conclusions: The concentrations of air pollutants in the survey area were found to be higher than the overall average in Busan. Based on this study, continuous air quality monitoring is necessary for residential areas near industrial complexes and ports. For further research, health biomonitoring of residents in these areas should be conducted to assess their exposure levels.

• PNF and Movement
• /
• v.19 no.3
• /
• pp.423-433
• /
• 2021

Purpose: Proprioceptive neuromuscular facilitation (PNF) is a method for promoting functional movements by facilitating neuromuscular responses through the stimulation of proprioceptors in the body using spiral and diagonal patterns. Irradiation, a basic principle of PNF, is a phenomenon in which the muscle activity of a body part caused by resistance is increased or spread into muscles in other parts via their connected muscles. Resistance training can be divided by body alignment into closed and open chain exercises. Methods: In this study, 19 healthy men in their 20s and 30s were selected as subjects. They performed PNF hip flexion, abduction, and internal rotation motions on their dominant side in an open chain exercise posture in which the nondominant sole was away from the wall, and in a closed chain exercise posture in which the sole was fixed to the wall. The nondominant leg's muscle activity was measured while resistance was maintained with applied pressure at 0%, 25%, 50%, 75%, and 100% of the maximum muscle strength in the last range of motions. A two-way analysis of variance (ANOVA) was conducted for a comparative analysis of the contralateral leg's muscle activity according to the chain exercise postures and the intensity of resistance intensity during PNF hip flexion, abduction, and internal rotation. In addition, an independent sample T-test was conducted for a comparative analysis of each chain exercise posture according to the intensity of resistance. A one-way ANOVA and a Scheffe post-hoc test were also performed to analyze the contralateral leg's muscle activity according to the intensity of resistance in the closed and open chain exercise postures. Results: Results of the two-way ANOVA found that the gluteus medius and the biceps femoris had statistically significant differences in both the chain exercise postures and resistance intensity (p<0.05), and that the vastus medialis and the gastrocnemius did not exhibit statistically significant differences in the chain exercise postures (p>0.05) but showed statistically significant differences in resistance intensity (p<0.05). As a result of the independent sample T-test, the application of the PNF hip flexion-abduction-internal rotation pattern led to a statistically significant difference in the contralateral gluteus medius during the closed chain exercise posture (p<0.05). According to the results of the one-way ANOVA and the Scheffe post-analysis, statistically significant differences were observed in the gluteus medius at 50%, biceps femoris at 75%, vastus medialis at 100%, and gastrocnemius at 100% during the closed chain exercise posture based on a resistance intensity of 0% (p<0.05). In the open chain exercise posture, statistically significant differences were found in the gluteus medius at 50%, biceps femoris at 50%, and vastus medialis at 75% based on the resistance intensity of 0% (p<0.05). In the same posture, there was no significant difference in the gastrocnemius's resistance intensity (p>0.05). Conclusion: When the PNF leg pattern is applied, each muscle requires effective chain exercise postures and resistance intensity to generate the contralateral leg muscle's irradiation.

• Korean Journal of Environment and Ecology
• /
• v.28 no.4
• /
• pp.472-483
• /
• 2014

Major cities have strengthened their efforts to improve the environmental function of the urban forest through aspects of quantity and quality. Yet, it is difficult to propose both long-term improvement and management policy direction continuously due to a lack of evaluating method that social needs could be reflected. After all, effective strengthening of the function of an urban forest, composed of social and biotic environments, is essential to create evaluation criteria. Such data reflects a quantity variation and identifies the distribution and habitation of biotic sources as well. An assessment of utilization possibilities presents such values as a green connectivity, an ecological healthiness, and usage and access opportunities. Consequently, this research was executed to create an urban forest sustainability index that proposes a management and policy direction based on the evaluation of quantity and quality aspects. This study was processed by the following steps: a degree of importance of ecological aspect, a sub-classification index choice, and an examination of adequacy. This research was conducted from premises that urban forest had another functions comparisons to a natural forest. As a result, a criteria of sustainability should be considered to evaluate an ecological aspect, as well as the social and management aspects regarding human disturbance. This research is designed to develop an evaluation index for ecological aspect. In order to evaluate the ecological healthiness of an urban forest, a vegetation index considers aspects of related rarity, damage possibility, naturalness, area, and diversity. In a wildbird index-related study, forest duration and area were selected. Finally, a variance analysis is presented, inclusive of stability, naturalness, diversity, potentiality, and possibility of wildlife inhabitation, which is indicative of an urban forest sustainability index.

• Imaging Science in Dentistry
• /
• v.36 no.2
• /
• pp.89-94
• /
• 2006

Purpose : To evaluate clinical usefulness of facial soft tissue thickness measurement using 3D computed tomographic images. Materials and Methods : One cadaver that had sound facial soft tissues was chosen for the study. The cadaver was scanned with a Helical CT under following scanning protocols about slice thickness and table speed; 3 mm and 3 mm/sec, 5 mm and 5 mm/sec, 7 mm and 7 mm/sec. The acquired data were reconstructed 1.5, 2.5, 3.5 mm reconstruction interval respectively and the images were transferred to a personal computer. Using a program developed to measure facial soft tissue thickness in 3D image, the facial soft tissue thickness was measured. After the ten-time repeation of the measurement for ten times, repeated measure analysis of variance (ANOVA) was adopted to compare and analyze the measurements using the three scanning protocols. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant intraobserver differences in the measurements of the facial soft tissue thickness using the three scanning protocols (p>0.05). There were no statistically significant differences between measurements in the 3 mm slice thickness and those in the 5 mm, 7 mm slice thickness (p>0.05). There were statistical differences in the 14 of the total 30 measured points in the 5 mm slice thickness and 22 in the 7 mm slice thickness. Conclusion : The facial soft tissue thickness measurement using 3D images of 7 mm slice thickness is acceptable clinically, but those of 5 mm slice thickness is recommended for the more accurate measurement.

• Journal of Korean Academy of Nursing
• /
• v.30 no.4
• /
• pp.880-892
• /
• 2000

The purpose of this study was to investigate the factors influencing health perception in the elderly, to provide the basic data for health behavior program and nursing intervention. The subjects of this study were 240 elderly person over the age 60, living in Seoul and Kangnung. They were conveniently sampled for this study and the data was collected from June 1999 to September 1999. The instruments for this study were the Health Perception Questionaire developed by Ware(1979), the OARS Functional Assessment Questionaire (Duke University 1978), and Multidimensional Health Locus of Control by Wallston, Wallston, and De Vills(1978). The data were analyzed by using SPSS Win computer Program. The results are as follows; 1. The total mean score of the health perception was 52.02(S.D=${\pm}$7.07) in a range of 33 to 69, and the mean score of the functional status was 27.02 (SD=${\pm}$2.75) in a range of zero to 28 and the mean score of the health locus of control was 65.66(S.D=${\pm}$8.68) in a range of 43 to 90. And The mean scores on the HLOC subscales were HLOC-I: 23.73${\pm}$4.56 (range: 6-30), HLOC-P: 23.07${\pm}$4.74 (range: 6-30), HLOC-C: 18.55${\pm}$4.03 (range: 11-30). 2. There was a significant positive correlation between health perception and functional status(r=.216, p=.001), and health perception and the health locus of control(HLOC) were not correlated at the level of statistical significance. However, the HLOC-I and health perception were correlated positively(r=.328, p=.000), and the HLOC-P were correlated negatively (r=-.129, p=.046). 3. There was a significant difference statistically in the degree of health perception according to the age(F=3.351, p=.002), spouse(t=2.232, p=.021), education level(F=7.373, p=.001), disease(t=3.639, p=.000), group activity (t=2.458, p=.015). drink(t=2.327, p=.021). 4. Stepwise multiple regression analysis revealed that the most powerful predictor of health perception was internal health locus of control. A combination of HOLC-I, functional status, HLOC-P, group activity explained 17.9% of the variance for health perception in the elderly. In conclusion, the results of this study show that internal health locus of control factor is very important in explaining the health perception for the elderly. Therefore, it will be considered internal health locus of control factor in nursing intervention and program in order to enforce the health behavior for elderly people .

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.6 no.1
• /
• pp.28-37
• /
• 1996

Benzidine, an aromatic amine used primarily in the manufacture of azo dyes, is recognized as a urinary bladder carcinogen in humans. In rats, mice, and hamsters, chronic exposure to benzidine resulted in tumors of the liver. The present study was undertaken to suggest analyzing the metabolites of benzidine with the optimal condition, identify the metabolites of benzidine, and observe time variance of the metabolites in the isolated perfusated rat liver. N-acetylbenzidine was synthesized by acetylation of benzidine with acetic anhydride and separated by thin layer chromatography(TLC) and high performance liquid chromatography(HPLC). To analysis benzidine and the metabolites of benzidine, HPLC operating condition has been optimized by means of preliminary experiment. The mobile phase consisted of acetonitrile(37%) in phosphate buffer, flow rate maintained at 1.0 ml/min. Optimal detective conditions were electrochemicaldetector(ECD) at 0.75 V for benzidine and N-acetylbenzidine and ultravioletdetector(UVD) at 287 nm for N,N'-diacetylbenzidine. The separation system was composed of a guard column and a separation column(Polymer C18, $4.6{\times}250cm$) at a temparature of $40^{\circ}C$. The perfusion system was equilibrated for 30 minutes before addition of benzidine to the perfusate. Samples of the perfusate were collected at time intervals(0, 10, 20, 30, 60, 90, 120 min) during the 2 hour perfusion. Before analyzing samples by HPLC/ECD/UVD, samples had been treated with sep-pak. Samples of perfusate analyzed by HPLC/ECD/UVD and the metabolites of benzidine in the isolated perfused rat liver were N-acetylbenzidine and N,N'-diacetylbenzidine. Benzidine metabolized over 60% during the initial 30 minutes of perfusion, extensively by 1 hour, and was undetectable in the perfusate. N-acetylbenzidine increased by 30 minutes of perfusion, declined. N,N'-diacetylbenzidine increased the 0-90 minutes period, remained constant during the 90-120 minutes period.