• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.805-813
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• 2018

Vanda is an aerial tropical orchid native to Thailand and nitrogen (N) fertilizer is mainly used to promote its growth and quality. However, little is known about the characteristics of N absorption and assimilation in Vanda. The objective of this study was to determine the appropriate source of N for Vanda cultivation. In this experiment, shoots and roots of Vanda 'Ratchaburi Fuchs-Katsura' were sprayed weekly with 100 ml of $^{15}N$ tracer solution (1) 10 mM of $^{15}NO_3{^-}$, (2) 5 mM of $^{15}NO_3{^-}$ plus 5 mM of $NH_4{^+}$, (3) 5 mM of $NO_3{^-}$ plus 5 mM of $^{15}NH_4{^+}$ and (4) 10 mM of $^{15}NH_4{^+}$. The results indicated that plants fed with a combined N fertilizer gave the highest of $^{15}N$ use efficiency ( $^{15}NUE$) of about 21.8%, 30 days after the first feeding (DAF), compared with those fed sole sources of $^{15}NO_3{^-}$ (21.0%) and $^{15}NH_4{^+}$ (16.6%). However, a sole nitrate fertilizer or combination fertilizer did not significantly affect the total N and labelled N content. Alanine was a major amino acid found in leaves and roots at 7 DAF, whereas glutamine was mainly found in stems. At 30 DAF, tyrosine and alanine became major components in the leaves, and glutamine decreased in stems when plants were fed with a single $^{15}NH_4{^+}$ source.

• Korean Journal of Plant Taxonomy
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• v.44 no.1
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• pp.39-50
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• 2014

Phylogenetic positions of Sedirea and Neofinetia were addressed using the chloroplast matK and the nuclear ITS sequences. We also evaluate the usefulness of the makers for the identification of species and localities. Sedirea and Neofinetia form an independent monophyletic genus, respectively, in both matK and nuclear ITS trees. The sister genus of the Neofinetia was Vanda in both trees. In addition, our trees support the separate recognition of the Neofinetia from Vanda rather than the inclusion of Neofinetia into Vanda. The sister group of the Sedirea was (Dimorphorchis(Pteroceras(Saccolabiun+Phalaeonopsis))) clade. The Dimorphorchis was one of the most probable sister genus to the Sedirea. The sister group relationship between Sedirea and Aerides was suggested by their similar morphology, but not supported in molecular trees. The identification of species and localities of Neofinetia was possible using our two molecular markers. However, several pseudo-gene sequences are discovered from the public data base. In addition, the horizontal gene transfer of chloroplast genomes is frequent events in orchid hybrids. Therefore, we need a careful evaluation for the data prior to systematic use. Generation of sequence data from multiple accessions of a species may helpful to reduce these types of error.

• Journal of Cardiovascular Imaging
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• v.31 no.2
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• pp.121-123
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• 2023

• Plant Biotechnology Reports
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• v.2 no.2
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• pp.145-152
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• 2008

Aerides vandarum and Vanda stangeana are two rare and endangered vandaceous orchids with immense floricultural traits. The intergeneric hybrids were synthesized by performing reciprocal crosses between them. In vitro germination response of the immature hybrid embryos was found to be best on half-strength Murashige and Skoog medium supplemented with 20% (v/v) coconut water/liquid endosperm from tender coconut. Determination of hybridity was made as early as the immature seeds or embryos germinated in vitro, using randomly amplified polymorphic DNA (RAPD) markers. Out of 15 arbitrarily chosen decamer RAPD primers, two were found to be useful in amplification of polymorphic bands specific to the parental species and their presence in the reciprocal crosses. However, a decisive profile that can identify the reciprocal crosses could not be provided by RAPD. Amplification of the trnL-F non-coding regions of chloroplast DNA of the parent species and hybrids aided easy identification of the reciprocal crosses from the fact that maternal inheritance of chloroplast DNA held true for these intergeneric hybrids. Subsequent restriction digestion of the polymerase chain reaction (PCR) amplified trnL-F non-coding regions of chloroplast DNA also consolidated the finding. Such PCR-based molecular markers could be used for early determination of hybridity and easy identification of the reciprocal crosses.

• Korean Journal of Radiology
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• v.22 no.8
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• pp.1310-1322
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• 2021

Immunotherapy is an effective treatment option for gynecological malignancies. Radiologists dealing with gynecological patients undergoing treatment with immune checkpoint inhibitors should be aware of unconventional immune-related imaging features for the evaluation of tumor response and immune-related adverse events. In this paper, immune checkpoint inhibitors used for gynecological malignancies and their mechanisms of action are briefly presented. In the second part, patterns of pseudoprogression are illustrated, and different forms of immune-related adverse events are discussed.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.2
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• pp.154-163
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• 2017

This study aims to investigate the utilization status of dental services by foreign children living in Seoul and their level of satisfaction with the treatments. We developed a structured questionnaire with 35 questions, which comprised 14 questions (demographic characteristics) and 21 questions (oral hygiene and dental experience of the child). In this study, the distribution of nationality of 391 participants differed from the actual statistics in Korea. It consisted of high percentage of participants from African countries (23.6%), as well as North American countries (24.1%). In addition, the education status of most parents was above the level of college graduate. Despite the relatively high socioeconomic status of the participants, they showed minimal dental health knowledge regarding the aspect of oral hygiene. Utilization of dental health services and the types of dental clinics visited by them were similar before and after coming to Korea. The participants showed overall satisfaction with the care, but many of them expressed the need for improvement with regard to treatment fee and communication. In conclusion, foreign children living in Seoul require appropriate dental health education and governmental support to promote regular dental check-ups, to improve their overall oral health, and prevent the incidence of dental caries.

• Asian-Australasian Journal of Animal Sciences
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• v.3 no.3
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• pp.161-164
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• 1990

Ninety six Rambouillet ${\times}$ Kaghani intact male lambs of 18 months of age were divided into 6 groups of 16 lambs each, groups being G-1, G-2, G-3, G-4, G-5 and G-6. Average liveweight of animals on different treatments at day 0 of the experiment was G-1 (24.68 S.D 3.35), G-2 (24.56 S.D 2.55), G-3 (24.53 S.D 2.90), G-4 (24.51 S.D 3.38), G-5 (24.58 S.D 3.58) and G-6 (24.81 S.D 3.43). Animals on treatment G-1 were fed only maize silage ad libitum, G-2 had been offered maize silage ad libitum plus 4.8 kg of commercial concentrate (Sona Vanda) per group, G-3 maize silage ad libitum plus 4.8 kg of crushed maize grain per group. G-4 lambs were fed oat silage ad libitum plus 4.8 kg of crushed maize per group, G-5 oat silage plus 4.8 kg of commercial concentrate per group and G-6 only oat silage ad libitum. The results showed highly significant differences (p<0.01) among treatment groups in silage intake throughout the feeding trial. G-2 group showed the highest silage intake while treatment G-4 showed the lowest silage intake for the first two months and treatment G-6 for the last two months. The results of growth rates (g/d/animal) showed that treatments G-1 and G-6, where no supplementation was given, had a weight loss throughout the feeding trial. However, weight loss in treatment G-6 was more severe than treatment G-1. Treatments G-2, G-3, G-4 and G-5 all showed weight gain however, treatment G-2 had the most gain. G-2 group also showed the highest wool production while G-6 the lowest. These results indicated that silage when made from full bloom crops of oats and silage fed alone without any supplementation causes weight loss in sheep. Supplementation with concentrates having 19% CP is far better than the crushed maize grain and maize silage is better than oats silage. Maize silage is superior than oat silage however, better performance could be expected if silages were made at the early bloom (dough) stage of plant maturity. The results indicate that treatment G-2 shows highest response while treatment G-6 the lowest. So the response of different treatments on both the parameters of weight gain and wool production is almost similar.