• Title/Summary/Keyword: VanB

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Comparison of TiO2 and ZnO catalysts for heterogenous photocatalytic removal of vancomycin B

  • Lofrano, Giusy;Ozkal, Can Burak;Carotenuto, Maurizio;Meric, Sureyya
    • Advances in environmental research
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    • v.7 no.3
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    • pp.213-223
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    • 2018
  • Continuous input into the aquatic ecosystem and persistent structures have created concern of antibiotics, primarily due to the potential for the development of antimicrobial resistance. Degradation kinetics and mineralization of vancomycin B (VAN-B) by photocatalysis using $TiO_2$ and ZnO nanoparticles was monitored at natural pH conditions. Photocatalysis (PC) efficiency was followed by means of UV absorbance, total organic carbon (TOC), and HPLC results to better monitor degradation of VAN-B itself. Experiments were run for two initial VAN-B concentrations ($20-50mgL^{-1}$) and using two catalysts $TiO_2$ and ZnO at different concentrations (0.1 and $0.5gL^{-1}$) in a multi-lamp batch reactor system (200 mL water volume). Furthermore, a set of toxicity tests with Daphnia magna was performed to evaluate the potential toxicity of oxidation by-products of VAN-B. Formation of intermediates such as chlorides and nitrates were monitored. A rapid VAN-B degradation was observed in ZnO-PC system (85% to 70% at 10 min), while total mineralization was observed to be relatively slower than $TiO_2-PC$ system (59% to 73% at 90 min). Treatment efficiency and mechanism of degradation directly affected the rate of transformation and by-products formation that gave rise to toxicity in the treated samples.

Phenotypic and Genotypic Differences of the Vancomycin-Resistant Enterococcus faecium Isolates from Humans and Poultry in Korea

  • Oh, Jae-Young;An, Seung-Hun;Jin, Jong-Sook;Lee, Yoo-Chul;Cho, Dong-Teak;Lee, Je-Chul
    • Journal of Microbiology
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    • v.45 no.5
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    • pp.466-472
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    • 2007
  • A total of 98 vancomycin-resistant Enterococcus faecium (VREF) isolates (58 isolates from patients and 40 isolates from poultry) were compared based on their antimicrobial susceptibility, Tn1546 element organization, and pulsed-field gel electrophoresis (PFGE) patterns. This comparison aided in determining the relationships between the groups of isolates. All the VREF isolates harbored the vanA gene; however, 29 (29.6%) of the isolates exhibited the VanB phenotype-vanA genotype. Furthermore, the VREF isolates from humans and poultry exhibited distinct antimicrobial resistance patterns. The PCR mapping of the Tn1546 elements exhibited 12 different transposon types (A to L). The VREF isolates of poultry were classified into types A to D, whereas the human isolates were classified into types E to L. A PFGE analysis demonstrated a high degree of clonal heterogeneity in both groups of isolates; however, the distinct VREF clones appeared in each group of isolates. The deletion of the vanX-vanY genes or insertion of IS1216V in the intergenic region from the vanX-vanY genes is directly associated with the incongruence of the VanB phenotype-vanA genotype in human VREF isolates. These data suggest that the VREF isolates exhibit distinct phenotypic and genotypic traits according to their origins, which suggests that no evidence exists to substantiate the clonal spread or transfer of vancomycin resistance determinants between humans and poultry.

A NOTE ON THE MIXED VAN DER WAERDEN NUMBER

  • Sim, Kai An;Tan, Ta Sheng;Wong, Kok Bin
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.6
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    • pp.1341-1354
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    • 2021
  • Let r ≥ 2, and let ki ≥ 2 for 1 ≤ i ≤ r. Mixed van der Waerden's theorem states that there exists a least positive integer w = w(k1, k2, k3, …, kr; r) such that for any n ≥ w, every r-colouring of [1, n] admits a ki-term arithmetic progression with colour i for some i ∈ [1, r]. For k ≥ 3 and r ≥ 2, the mixed van der Waerden number w(k, 2, 2, …, 2; r) is denoted by w2(k; r). B. Landman and A. Robertson [9] showed that for k < r < $\frac{3}{2}$(k - 1) and r ≥ 2k + 2, the inequality w2(k; r) ≤ r(k - 1) holds. In this note, we establish some results on w2(k; r) for 2 ≤ r ≤ k.

Detection of Vancomycin-Resistant Enterococci and Related Genes Using VITEK 2 System and Multiplex Real-time PCR Assay (VITEK 2 시스템과 Multiplex Real-time PCR을 이용한 반코마이신 내성 장알균(VRE)과 내성관련 유전자 검출)

  • Jeong, Min-Kyung;Yu, Young-Bin;Kim, Sang-Ha;Kim, Sunghyun;Kim, Young-Kwon
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.4
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    • pp.401-406
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    • 2017
  • In this study, using the VITEK 2 system, 74 samples (22.6%) out of 327 specimens were identified by the growth of Enterococcosel media (EV6 agar) supplemented with $6{\mu}g/mL$ of vancomycin. Enterococcus faecium was identified as 55 strains (74.3%), Enterococcus casseliflavus as 2 strains (2.7%), Enterococcus avium as 1 strain (1.4%), and Enterococcus gallinarum as 16 strains (21.6%). Among the 55 phenotypes of Enterococcus faecium, 42 (76.4%), 9 (16.4%), and 4 strains (7.3%) showed the vanA, vanB, and vanC phenotype, respectively. The 16 strains of Enterococcus gallinarum and 2 strains of Enterococcus casseliflavus showed the vanC phenotype and the 1 strain of Enterococcus avium had the vanB phenotype. The one strain of Enterococcus faecium propagated only in EV4 and was susceptible to both vancomycin and teicoplanin according to the antimicrobial susceptibility test using the VITEK 2 system. The vancomycin resistance phenotype gene was not detected by PCR. A total of 327 specimens were cultured in Enterococcosel broth supplemented with $6{\mu}g/mL$ of vancomycin (EV6 broth), and 120 strains (36.7%) were isolated. These 120 strains were subjected to vancomycin resistant genotyping by a multiplex real-time polymerase chain reaction and 51 strains (42.5%) showed vanA; 5 strains (4.2%) showed vanA and vanC; and 18 strains (15%) showed vanC. Vancomycin resistance genotypes were not detected in the remaining 46 strains (38.3%).

High prevalence of Enterococcus spp. from dogs with otitis externa

  • Jo, Hyun-Jung;Chae, Hee-Sun;Kim, Hyun-Ju;Kim, Min-Ju;Park, Gyu-Nam;Kim, Sang-Hun;Chang, Kyung-Soo
    • Korean Journal of Veterinary Service
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    • v.35 no.2
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    • pp.99-104
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    • 2012
  • Otitis externa (OE) is a frequent disease in the ear canals of dogs. To identify the pathogens causing OE in dogs and to determine their antimicrobial resistances, specimens were collected from animal hospitals in Daejeon. The isolates were examined by morphological and biochemical tests, 16S rRNA analysis and antimicrobial susceptibility tests. We analyzed correlation between the isolated pathogens and external factors of dogs such as breed, age, gender, ear mite, hair in ears and experience with antibiotic therapy. Thirty three strains of bacteria were isolated from 26 of the 68 heads of dogs with OE. The most isolated bacteria were Enterococcus faecalis (E. faecalis) followed by Staphylococcus aureus (Sta. aureus), Sta. pseudointermedius, E. faecium, E. avium and Streptococcus canis (Strep. canis) in order of frequency of occurrence. Isolation frequency of Enterococcus spp. and Staphylococcus spp. were 51.5% and 45.5%, respectively. E. faecalis and E. faecium isolates showed VanB phenotype, which is resistant to vancomycin but sensitive to teicoplanin were 58% and 25%, respectively. Nine isolates among total twelve isolates of E. faecalis were isolated from the dogs treated with antibiotics. There was no methicillin-resistant Sta. aureus (MRSA), but were MR-Sta. pseudointermedius (MRSP) (57.1%) and vancomycin-resistant (VR)-Sta. pseudointermedius (14.3%) (VRSP) showing VanB phenotype. However, vanA, vanB and vanC genes were not detected in VR isolates from the dogs. Taken together, VR-Enterococcus spp. (VRE) is one of the major pathogens in domestic animals, as well as community-and hospital-acquired infection.

A Study on the Relation Between SOLO Taxonomy and van Hele Theory (SOLO 분류법과 van Hiele의 기하학습 수준 이론의 관련성에 대한 고찰)

  • 류성림
    • The Mathematical Education
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    • v.39 no.2
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    • pp.151-166
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    • 2000
  • The purpose of this study is to understand what two models of SOLO taxonomy and van Hiele theory suggest and find out what relation there is between the category system of the SOLO taxonomy and the thinking level of the van Hiele theory. The van Hiele theory describes in line of ranking level so that it may increase the teaching effects by putting together a class, which takes into consideration the students thoughts. The SOLO taxonomy focused on the response mode of the students rather than the thinking level or the developmental stage of them to pursuit the method that can describe the students understanding in depth quality-wise. Although the SOLO taxonomy and the van Hiele model seem to have different form and character from outside in terms of their goals, a closer examination reveals that the two stances have much in common and that the models are complementary. Although the van Hiele placed more focus on the thoughts, because the conclusion was based on the students responses, the van Hiele theory can be interpreted within the structure identified in the SOLO model. In this study, we have tried to understand how the response structure form the SOLO taxonomy and the thinking level of the van Hiele theory are related, based on the studies of Pegg and Davery1998). If you briefly look at them, there are following corresponding relation between the SOLO taxonomy and the van Hiele theory. a) The relational level(R) in iconic moe is van Hiele level 1. b) The multisturctural level(M$_2$) in the second cycle of concrete-symbolic mode is van Hiel level 2. c) The relation level(R$_2$) in the second cycle of concrete-symbolic mode is van Hiele level 3. d) The unistructural level(U$_2$) in the second cycle of formal mode is van Hiele level 4. e) The postformal mode is van Hiele levle 5. Though it would be difficult to conclude that these correspondences were perfectly done, if you look at their relation, you can see that the learning process of the students were not carried out uniformly. Therefore, by studying the students response structure, using the SOLO taxonomy, and identifying the learning cycle and understand the geometrical concept more in depth.

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A NEW q-ANALOGUE OF VAN HAMME'S (G.2) SUPERCONGRUENCE FOR PRIMES p ≡ 3 (mod 4)

  • Victor J. W. Guo;Xiuguo Lian
    • Bulletin of the Korean Mathematical Society
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    • v.60 no.3
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    • pp.775-783
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    • 2023
  • Van Hamme's (G.2) supercongruence modulo p4 for primes p ≡ 3 (mod 4) and p > 3 was first established by Swisher. A q-analogue of this supercognruence was implicitly given by the first author and Schlosser. In this paper, we present a new q-analogue of Van Hamme's (G.2) supercongruence for p ≡ 3 (mod 4).

A Method For Solving the Problem of Existing EDI System by Using XML (XML을 이용한 기존 EDI 시스템의 문제점 극복방안)

  • 장민석;김상중
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2001.05a
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    • pp.275-278
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    • 2001
  • The importance of EDI is growing more and more as the trend of E-Commerce is changing to B2B from B2C. So far WEB-EDI system using HTML has substituted for existing traditional VAN-EDI system. But the critical problem of WEB-EDI is that integrating among the internal systems in the company is difficult. In this paper we suggests a method for integrating the internal data and thus internal systems by using XML, a second generation markup language.

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Rotational State Distributions of I2(B) from Vibrational Predissociation of I2(B)-Ne

  • Cho, Sung-Sil;Sun, Ho-Sung
    • Bulletin of the Korean Chemical Society
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    • v.25 no.9
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    • pp.1397-1402
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    • 2004
  • The vibrational predissociation of triatomic, i.e., atom-diatom, van der Waals complexes in transient electronic excited state has been widely investigated. The predissociation rates or lifetimes are major concerns of the previous studies. Experimentally rotational state distributions of diatomic product are hardly investigated and few theoretical stuides on rotational state distributions have appeared in literature. In this work, choosing the frequently studied $I_2(B)-Ne$ complex as an example, we investigate the change of rotational state distributions of $I_2(B)-Ne$ produced from predissociation of the various initial states of $I_2(B)-Ne$. The present study on the rotational distributions indicates that rotational state distributions depend significantly on the predissociation energy and the van der Waals vibrational modes of $I_2(B)-Ne$. That is, the initial state dependency of rotational state distributions is extensively discussed.