• Journal of Life Science
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• v.27 no.4
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• pp.417-422
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• 2017

The purpose of this study was to investigate the role of the Vaccinum oldhami fruit extract as a cosmetic additive. As a result of having macrophage (RAW 264.7) measured a cell toxicity effects of 70% ethanol extract from Vaccinum oldhami fruit, it shown 118% with toxicity at $500{\mu}g/ml$ concentration. In nitric oxide synthesis inhibition effect, 70% ethanol extracts from Vaccinum oldhami fruit shown 47.3% at $1,000{\mu}g/ml$ concentration. The iNOS, COX-2 protein expression inhibitory effect by western blot of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 36.13%, 29.61% at $500{\mu}g/ml$ concentration. And iNOS, COX-2 mRNA expression inhibitory effect by reverse-transcription-PCR of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 62.25%, 90.07% at $500{\mu}g/ml$ concentration. All these finding that extract from Vaccinum oldhami fruit could prove that their have effects anti-inflammatory efficacy. And extract from Vaccinum oldhami fruit has potential as a cosmetic ingredients.

• Journal of Life Science
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• v.33 no.2
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• pp.115-128
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• 2023

The fruit of Vaccinum oldhami was separated and purified to obtain the compounds chlorogenic acid (CA), quercetin (QT), and quercitrin (QR). The electron-donating abilities of CA, QT, and QR at 1,000 ㎍/ml were 91.9%, 89.9%, and 77.4%, respectively QT and QR showed 99.5% and 91.4% ABTS⁺ radical scavenging ability at a 1,000 ㎍/ml concentration, respectively, and CA showed a 95% ability or higher at 100 ㎍/ml. Regarding tyrosinase inhibitory activity, CA, QT, and QR exhibited 29.5%, 34.7%, and 23.7% efficacy, respectively, at 1,000 ㎍/ml. Regarding the cell viability for melanoma cells (B16F10) assessed through MTT assay, CA, QT, and QR showed cell a viability of 80% or more at 100 ㎍/ml. To measure the deterrent of protein expression, CA affected TRP-1 and TRP-2 in accordance with increases in concentration. The protein expression inhibition rate of QT was excellent for TRP-1, TRP-2, and tyrosinase. CA was confirmed to have an excellent mRNA expression inhibitory effect against MITF, and the amount of mRNA expression of TRP-1, TRP-2, and tyrosinase decreased with an increase in the CA concentration. As the concentration of QT increased, the mRNA expression of MITF, TRP-2, and tyrosinase decreased. QR decreased the amount of mRNA as the QR concentration increased. The excellent antioxidant and whitening effects of CA, QT, and QR were thus confirmed.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.392-399
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• 2018

Fruits of 10 cultivars ('Blue Gold', 'Brigitta', 'Coville', 'Duke', 'Nelson', 'North Blue', 'Rancocas', 'Sierra', Sunrise, and 'Weymouth') of blueberries (Vaccinum corymbosun) were analyzed for characteristics, contents of total phenolic compounds and flavonoids and antioxidant activity in this study. Fruit weights ranged from 0.83 to 1.88 g. Total soluble solids concentration varied from 9.7 in 'Duke' to $16.6^{\circ}Brix$ in 'Sierra' with titratable acidities of 0.94 % in 'Sunrise' and 1.75% in 'Brigitta'. There are relatively high contents in 'North Blue' ($23.75mg\;GAE{\cdot}g^{-1}\;FW$) and low contents in 'Coville' ($17.15mg\;GAE{\cdot}g^{-1}\;FW$) in total phenolic compounds. Contents of total phenolic compounds were high in 'Nelson'($14.1mg\;QE{\cdot}g^{-1}\;FW$) and low in 'Duke' ($10.1mg\;QE{\cdot}g^{-1}\;FW$). Analysis of antioxidant activity of blueberry fruits showed that there were high acitiviites of ABTS+ radical scavenging in 'Rancoccas' (82.2%), 'Bluegold' (79.6%), and 'Nelson' (77.8%), and high activities of DPPH radical scavenging in 'Rancocca' (76.0%), and high in hydroxy radical scavenging in 'Nelson' (73.0%). Quantification analysis method of qualitative data showed that 'Bluegold', 'Nelson', 'Northblue', and 'Rancocas' had high contents of phenol and flavonoid compounds, and activity antioxidants of berries. Blueberry cultivars selected by statistical quantification analysis can be utilized as valuable genetic resources for breeding of blueberry with high antioxidant activities in the future.