• Journal of Gastric Cancer
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• 제20권4호
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• pp.385-394
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• 2020

Purpose: Patients with gastric cancer who receive neoadjuvant therapy are staged before treatment (cStage) and after treatment (ypStage). We aimed to compare the prognostic reliability of cStage and ypStage, alone and in combination. Materials and Methods: Data for all patients who received neoadjuvant therapy followed by surgery for gastric adenocarcinoma from 2004 to 2015 were extracted from the National Cancer Database. Kaplan-Meier (KM)curves were used to model overall survival based on cStage alone, ypStage alone, cStage stratified by ypStage, and ypStage stratified by cStage. P-values were generated to summarize the differences in KM curves. The discriminatory power of survival prediction was examined using Harrell's C-statistics. Results: We included 8,977 patients in the analysis. As expected, increasing cStage and ypStage were associated with worse survival. The discriminatory prognostic power provided by cStage was poor (C-statistic 0.548), while that provided by ypStage was moderate (C-statistic 0.634). Within each cStage, the addition of ypStage information significantly altered the prognosis (P<0.0001 within cStages I-IV). However, for each ypStage, the addition of cStage information generally did not alter the prognosis (P=0.2874, 0.027, 0.061, 0.049, and 0.007 within ypStages 0-IV, respectively). The discriminatory prognostic power provided by the combination of cStage and ypStage was similar to that of ypStage alone (C-statistic 0.636 vs. 0.634). Conclusions: The cStage is unreliable for prognosis, and ypStage is moderately reliable. Combining cStage and ypStage does not improve the discriminatory prognostic power provided by ypStage alone. A ypStage-based prognosis is minimally affected by the initial cStage.

• 운동영양학회지
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• 제25권3호
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• pp.16-22
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• 2021

[Purpose] Exercise-associated hyponatremia (EAH) is a well-known condition among endurance athletes at low altitudes. The incidence of EAH during ultramarathons at high altitudes warrants further investigation. This prospective observational study was conducted on the participants of the Leadville Trail 100 run, a 161-km race held at a high altitude (2,800 m-3,840 m). [Methods] Venous blood samples were collected before and immediately after the race. The participants completed an electronic survey after the race. Our main outcome measure was the post-race serum sodium ([Na⁺]) level. [Results] Of the 672 athletes who started the race, 351 (52%) successfully completed the event within the 30-hour cut-off. Post-race blood samples were collected from 84 runners (66 finishers). Both pre- and post-race blood samples were collected from 37 participants. Twenty percent of the post-race participants had EAH. Only one post-race participant had a [Na⁺] level of <130 mmol/L. All participants with EAH were asymptomatic. One participant had an abnormal pre-race [Na⁺] level (134 mmol/L). Female participants had a significantly higher rate of EAH than male participants (40% vs. 16%; p=0.039). Age, body mass index, weight changes, race completion status, nonsteroidal anti-inflammatory drug use, and urine specific gravity were not associated with the development of EAH. Lower postrace [Na⁺] levels were associated with higher serum creatine kinase values (R²=0.1, p<0.005). [Conclusion] High altitude (3,840 m peak) does not appear to enhance the incidence of EAH after an ultramarathon footrace. This suggests that ambient temperature (low temperatures reduce risk), sex (female predilection), endurance running, and overhydration are more prominent risk factors for EAH than high altitude.

• The Korean Journal of Physiology
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• 제24권2호
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• pp.345-352
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• 1990

To determine the role of sulfhydryl group in transport of organic ions across the basolateral membrane of renal proximal tubules, effect of p-chloromercuribenzoic acid (PCMB) on the transport of tetraethylammonium (TEA) and p-aminohippurate (PAH) was studied in rabbit renal cortical slices. PCMB caused irreversible inhibition of TEA and PAH uptake in a dose-dependent manner, with $I_{50}$ value (concentration for 50% inhibition) of $30\;{\mu}M$ for TEA and $75\;{\mu}M$ for PAH. Kinetic analysis of TEA and PAH uptakes showed that PCMB decreased Vmax $(62.35\;vs.\;28.32\;n\;mole/g{\cdot}min\;fur\;TEA:\;385.24\;vs.\;170.36\;n\;mole/g{\cdot}min\;for\;PAH)$ without changing Km. The inhibitory action of PCMB on TEA and PAH uptakes was independent of pH of the pretreatment medium. The inhibitory effect of PCMB on the uptake of TEA or PAH was prevented by dithiothreitol, but not by the substrate. PCMB inhibited Na-K-ATPase activity in a dose-dependent manner with $I_{50}$ value of $50\;{\mu}M$, which is similar to those for TEA and PAH uptake. These results suggest that PCMB inhibits the transport of organic cations and anions in the renal basolateral membrane by directly affecting the SH-group in the transporter molecules or secondly by altering the Na-K-ATPase activity.

• Journal of Trauma and Injury
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• 제34권2호
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• pp.87-97
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• 2021

Purpose: This study examined emergency medical service (EMS) transportation patterns for adult trauma patients before and after establishing a level 1 regional trauma center (RTC) and to evaluate the transportation approach after prehospital severity screening. Methods: This was a retrospective observational study of trauma patients aged ≥18 years admitted via EMS to the emergency department or a level 1 RTC, 1 year before to 3 years after RTC establishment. Patients with an Injury Severity Score (ISS) in the patient registration system were selected. Analyses were performed to determine transportation pattern changes by comparing patients pre- and post-RTC establishment and by yearly comparisons over the 4-year study period using the Mann-Whitney U test and chi-square test. Results: Overall, 3,587 patients were included. The mean ISS was higher in the post-RTC group (n=2,693; 10.63±8.90, median 9.00) than in the pre-RTC group (n=894; 9.44±8.20, median 8.00; p<0.001). The mean transportation distance (9.84±13.71, median 5.80 vs. 13.12±16.15 km, median 6.00; p<0.001) was longer in the post-RTC group than in the pre-RTC group. Furthermore, proportionally fewer patients were transported from an area in the same city as the RTC after establishment (86.1% vs. 78.3%; p<0.001). Yearly comparisons revealed a gradually increasing trend in the hospital death rate (ptrend=0.031). Conclusions: After establishing a level 1 RTC, the EMS transportation of severe trauma patients increased gradually along with the long-distance transportation of minor trauma patients. Therefore, improved prehospital EMS trauma severity assessments and level 1 RTC involvement in patient classification in the prehospital phase are necessary.

• Journal of Ecology and Environment
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• 제46권1호
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• pp.76-84
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• 2022

Background: Climate and livestock grazing are key agents in determining current Mongolian steppe vegetation communities. Together with plant coverage or biomass, palatability of steppe community is regarded as a useful indicator of grassland degradation, in particular, at grazing hotspots in arid and semi-arid grasslands. This study analyzed relationships between livestock grazing pressure and steppe vegetation palatability at three summer pastures with different aridity (dry, xeric, and mesic) and livestock numbers (1,100, 1,800, and 4,100 sheep units, respectively). At each site, it was surveyed coverage, biomass, and species composition of different palatability groups (i.e., palatable [P], impalatable [IP], and trampling-tolerant [TT]) along a 1-km transect from grazing hotspots (i.e., well) in every July from 2015 to 2018. Results: In results, total vegetation coverage increased with wetness, 7 times greater at mesic site than dry one in averages (33.1% vs. 4.5%); biomass was 3 times higher (47.1 g m^-2 vs. 15.7 g m^-2). Though P was the dominant palatability group, the importance of IP in total coverage increased with aridity from mesic (0.6%) to dry (40.2%) sites. Whereas, TT increased with livestock numbers across sites. Locally, IP was observed more frequently near the wells and its spatial range of occurrence becomes farther along the transects with aridity across sites from mesic (< 100 m) to dry (< 700 m from the well). Conclusions: Our results showed that the importance of IP and its spatial distribution are different at both local and regional scales, indicating that the palatability parameters are sensitive to discern balance between selective-grazing demand and climate-driven foraging supply in Mongolian rangelands.

• 사상체질의학회지
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• 제34권1호
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• pp.46-57
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• 2022

Background Adherence to healthy diet acts as a key role to modify sedentary lifestyle in real life setting. Constitution type of traditional Korean medicine has been prediagnosed to risk factors of cardiometabolic diseases. This study aims to evaluate the associations between body composition and healthy eating status in Korean adults by their constitution type. Methods Of 4046 participants from Korean Medicine Daejeon Citizen Cohort study, Korean Medicine Daejeon Citizen Cohort (KDCC) study were included for analysis. Demographic, health-related behaviors and Korean Medicine (KM) type were surveyed based on a general health-related questionnaire. Anthropometric measurements and dietary factors by using Korean Healthy Eating Index (KHEI) were assessed only in the half of the original participants. Results 50.8% of Taeeum was observed from eligible 1967 participants (66.7%, women). The highest KHEI score was observed in soyang group (52.0±0.3, p=0.006) compared to other two groups. In taeeum group, lower appendicular skeletal muscle mass (ASM)(%)(Taeeum: 38.7±0.1 vs. Soeum/Soyang: 39.2±0.1, p < 0.05), and higher percent of body fat (PBF)(%) (Taeeum: 32.9±0.2 vs. Soeum/Soyang: 29.0±0.2, p < 0.05) by the lowest tertile (T1) of the KHEI score, respectively. When the KHEI score increased by 1 point in taeeum group, a positive relationship was observed, which increased by 0.015% of ASM, however, a negatively related to in which body fat mass (BFM) decreased by 0.022 kg and PBF decreased by 0.024%. Conclusion Customized nutritional management is required that could help maintaining physical health and diet by their constitution type.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.153-153
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• 2022

Large-scale and accurate observations at fine spatial resolution through a means of remote sensing offer an effective tool for capturing rainfall variability over the traditional rain gauges and weather radars. Although satellite rainfall products (SRPs) derived using two major estimation approaches were evaluated worldwide, their practical applications suffered from limitations. In particular, the traditional top-down SRPs (e.g., IMERG), which are based on direct estimation of rain rate from microwave satellite observations, are mainly restricted with their coarse spatial resolution, while applications of the bottom-up approach, which allows backward estimation of rainfall from soil moisture signals, to novel high spatial resolution soil moisture satellite sensors over South Korea are not introduced. Thus, this study aims to evaluate the performances of a state-of-the-art bottom-up SRP (the self-calibrated SM2RAIN model) applied to the C-band SAR Sentinel-1, a statistically downscaled version of the conventional top-down IMERG SRP, and their integration for a targeted high spatial resolution of 0.01° (~ 1-km) over central South Korea, where the differences in climate zones (coastal region vs. mainland region) and vegetation covers (croplands vs. mixed forests) are highlighted. The results indicated that each single SRP can provide plus points in distinct climatic and vegetated conditions, while their drawbacks have existed. Superior performance was obtained by merging these individual SRPs, providing preliminary results on a complementary high spatial resolution SRP over central South Korea. This study results shed light on the further development of integration framework and a complementary high spatial resolution rainfall product from multi-satellite sensors as well as multi-observing systems (integrated gauge-radar-satellite) extending for entire South Korea, toward the demands for urban hydrology and microscale agriculture.

• ALGAE
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• 제30권2호
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• pp.89-101
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• 2015

Ecklonia cava Kjellman is a common kelp found in shallow subtidal in warm-temperate waters in the northwest Pacific Ocean. This species has shown substantial morphological variation along with subsistence in different locations and local environments. We quantified the magnitude of morphological variation of E. cava from six populations along ~700 km of coastline from Jeju Island to Dokdo in Korea. In addition, we examined genetic distance among the populations using random amplified polymorphic DNA (RAPD) analysis. Most morphological characteristics investigated were significantly different among locations. Multivariate analyses indicated two phenetically distinct groups (nearshore, sheltered vs. offshore, exposed), indicating wave exposure with turbidity are presumably major factors for the separation. With RAPD data, results of Nei's diversity (H) and AMOVA showed considerable variations in within- and between-populations. Pairwise ${\Phi}_{ST}$ and $N_m$ values indicated moderate gene flow between the six locations. Results of Nei's analysis revealed three genetically distinct groups, not consistent with the morphological groupings, indicating that a time gap may exist between morphological and genetic variations. This study also suggests dispersal distance of this kelp may be longer than what is commonly thought and genetic similarity in the populations was largely reflected by the direction of ocean current rather than just geographical distance.

• 생명과학회지
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• 제25권5호
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• pp.507-514
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• 2015

D-Xylulose는 nonoxidative pentose phosphate 경로를 통해 glycolysis 과정으로 들어가기 전에 D-xylulose kinase에 의해서 D-xylulose-5-phosphate로 인산화 된다. K. gwangalliensis strain SJ2로부터 D-xylulose kinase (XK)를 암호화하는 유전자는 E. coli를 이용하여 서열분석 및 발현 하였으며, XK 유전자의 염기서열 1,419 bp로 구성되어 있으며 463개의 아미노산 잔기를 암호화하고 있다. 분석결과를 통해 XK 유전자가 진화과정 동안 잘 보존되었음을 보여 주었다. XK 유전자의 발현을 위해 pCold-II 발현 벡터에 클로닝 하였으며 클로닝 된 플라스미드는 E. coli strain BL21 (DE3)에 형질전환 하여 IPTG를 이용해 발현을 유도하였다. 재조합 된 XK 단백질의 크기는 약 48 kDa이었다. 이 발현된 단백질은 affinity chromatography를 이용하여 정제하였으며 D-xylulose kinase에 따른 enzymatic activity를 분석하였다. D-xylulose와 ATP로 실행한 XK enzyme kinetic 연구는 각각 250±20 μM과 1,300±50 μM의 Km value를 보였다. 본 연구를 통해 얻어진 결과는 분자적 수준에서 D-xylulose kinase의 특성연구의 보다 넓은 지식적 기초를 제공할 것으로 사료된다.

• The Korean Journal of Physiology
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• 제27권2호
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• pp.209-215
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• 1993

The aim of the present study was to derive regression equations for $\dot{V}o_{2max}\;vs.\;\dot{V}o_{2peak},\;and\;\dot{V}o_2\;vs.$ heart rate, exercise time, and other variables from maximal exercise tests on a treadmill using the Bruce and inclined protocols. Twelve male and 10 female Korean college students aged between 19 and 23 years voIunteered for this study. After the resting measurements, the subjects performed a maximal exercise on a treadmill according to the Bruce protocol. When the resting conditions were restored, the subjects performed another maximal exercise according to an inclined protocol where the speed was fixed at 8.05 $km{\cdot}h^{-1}$ and the grade was incremented starting from 09t by 2.5% for every 2 min. Peak $\dot{V}o_2$ observed during the Bruce exercise $(\dot{V}o_{2peak})$ was $37.7{\pm}2.4\;and\;31.7{\pm}1.8\;ml\;kg^{-1}\;min^{-1}$ in the male and female groups, respectively. Peak $\dot{V}o_2$ observed during the inclined exercise was higher than $\dot{V}o_{2peak}$ during the Bruce exercise. Maximum $\dot{V}o_2$ value observed during the tyro exercises $(\dot{V}o_{2max})$ was $43.0{\pm}2.8\;and\;36.2{\pm}1.4\;ml\;kg^{-1}\;min^{-1}$ in the male and female groups, respectively. Thus, $\dot{V}o_{2peak}$ by the Bruce protocol was about 12% (male) or 13% (female) lower than $\dot{V}o_{2max}$, and a linear relationship was found between $\dot{V}o_{2peak}$ and $\dot{V}o_{2max}$. The peak values of % $\dot{V}o_{max}$ with the Bruce protocol were $89.2{\pm}3.3\;and\;87.5{\pm}3.6%$ and those with the inclined protocol $97.7{\pm}1.8\;and\;96.9{\pm}2.0%$ in the male and female groups, respectively. In the female group, $%\dot{V}o_{2max}$ at a given workload was higher than in the male group, while $\dot{V}o_{2}$ per kg body weight was the same. Maximum HR observed during the two exercises was $204{\pm}2\;and\;195{\pm}3\;beat\;min^{-1}$ in the male and female groups, respectively. Linear relationships were found, excluding the last points, between 1) $\dot{V}o_{2}$ and exercise time, 2) $\dot{V}o_{2}$ and $%\dot{V}o_{2max}\;and\;%HR_{max}$.