• Title/Summary/Keyword: VR299

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Characterization of the Infectious Pancreatic Necrosis Virus (IPNV) isolated from Pan-Cultured Rainbow Trout in Korea (한국산 양식송어에서 분리된 전염성 췌장괴저 바이러스의 특성)

  • 박정우;이정진;정가진;하영칠
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.225-230
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    • 1989
  • Infectious Pancreatic Necrosis Virus (IPNV) is one of the most important pathogens for inland fish farming and had been first reported in Korea from returning adult chum salmon (Oncorhynchus keta) at hatcheries on the east coast. During the past years, several viruses identified as IPNV were isolated not only from chum salmon, but also from gold fish (Carassius auratus), eel(Anguilla japonica), and rainbow trout (Salmo gairdneri). An isolate, coded DRT, from fingerlings of pan-cultured rainbow trout in Daechung Dam showed different serotype from three known reference serogroups of IPNV such as VR-299, Sp, and Ab. Antisera to three of these serotypes, however, partially neutralized the infectivity of this isolate. Anti-Sp type was rather effective than either anti-VR-299 or anti-Ab, implying DRT could be more closely related to Sp. DRT has been purified and its RNA genome segments were compated showing that the isolate does not belong to any of known serogroups even with some common antigenicity.

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Isolation and identification of infectious pancreatic necrosis virus form rainbow trout cultured in Korea (Isolation and Identification of Infectious Pancreatic Necrosis Virus from Rainbow Trout Cultured in Korea)

  • Lee, Jin-Hee;Heo, Gang-Joon;Lee, Chan-Hee
    • Journal of Microbiology
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    • v.34 no.2
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    • pp.124-130
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    • 1996
  • A survey was conducted to determine the prevalance of infectious pancreatic necrosis virus (IPNV) on fish farms in Korea and the epidemiology of IPNV infection in the farmed rainbow trout. In total, 43 pools of rainbow trout with apparent signs of viral infection from five provinces were obtained and analyzed. Evident cytopathic effects, including karyopycnosis and cell destruction, were observed in CHSE (chinook samlmon embyro)-214 cells infected with the virus isolates. Of these, ten viral isolates were assumed to be IPNV based on biophysical properties. RNA analysis revealed that the isolates contained two-segmented RNA genomes, further indicating that the viral isolates are IPNV. Antigenic comparison of the IPNV isolates identified three distinct serological groups separable by the cross-neutralization test. Of the ten IPNV isolates, six could be classified as strain DRT, two as strain Ab, and two as strain VR299. We were not able to isolate new strain of IPNV or any isolate serologically similar to the standard strain Sp.poraceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

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A Study of Introducing Virtual Reality for Fire Disaster Preparedness Training (화재재난 대비훈련을 위한 가상현실의 도입방안 연구)

  • Kim, Jong Kouk;Han, Dong-Ho
    • The Journal of the Convergence on Culture Technology
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    • v.4 no.1
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    • pp.299-306
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    • 2018
  • As recent Jecheon and Milyang fire cases show, the need for fire disaster training to prepare for a fire disaster continues to grow. In the event of a disaster, people become mentally confused and are called disaster personalities. In order to survive in a disaster, it is necessary to develop the power to overcome the disaster personality by experiencing the disaster situation in advance. Therefore, training to overcome disaster personality is needed, and virtual reality can be a good training means in that it can experience without physical space. In addition, periodic actual disaster evacuation drills should be carried out to compensate for the shortcomings of virtual reality. In order to introduce fire disaster drill using virtual reality and to spread it to the public, the Korea National VR project should be introduced which benchmarked national PC project which succeeded in the past informatization project. Besides, the Korea National Safety Point system should be integrated to cover disaster preparedness training and building reinforcement. If the national VR project and the national safety point system are introduced successfully, Korea will be the basis for escaping the disgraceful nickname of 'disaster republic'.

Biological and Serological Characteristics of Birnavirus Isolated from Cultured Japanese Flounder in 1999 (양식산 넙치 치어로부터 분리한 버나바이러스의 Marine Birnavirus(MBV)와 Infectious Pancreatic Necrosis Virus(IPNV)와의 연관성)

  • Oh, Myung-Joo;Jung, Sung-Ju;Kim, Hyeung-Rak
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.56-62
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    • 1999
  • Since 1998, mass mortality of the Japanese flounder has widely occurred in the south and west coastal area of Korea. A new serotype birnavirus was isolated during the investigation of the cause of the disease. By the electromicroscopic examination, the isolated virus particles appeared hexagonal and unenveloped with an average diameter 52 to 56 nm. Birnavirus specific fragment was amplified by RT-PCR. High yield of virus (10.3 to 10.8 log $TCID_{50}/ml$) was produced in CHSE-214. RTG-2 and RTE-2 cells. Typical birnavirus CPE was observed in these cells. On the contrary, the virus CPE was not shown in the FHM and EPC cells. By a cross-neutralization test with IPNV Ab, IPNV Sp, IPNV VR-299 and MBV Y6, the isolated virus was closely related to marine birnavirus (MBV).

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A new serotype confirmed by partial physical mapping of cDNA clones from the infectious pancreatic necrosis virus (IPNV) isolated in Korea (한국에서 분리된 전염성 췌장괴저 바이러스의 새로운 혈청형에 대한 유전자 분석)

  • 이정진;박정우;정가진
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.231-236
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    • 1989
  • The larger segment of double stranded RNA genome from a new serotype of Infectious Pancreatic Necrosis Virus (IPNV), DRT, has been partially cloned at Sma I site in pUC19 and compared with the restriction maps of VR-299 and Sp. Restrction sites found in DRT was distinct and hence a new serotype. The cDNA clones of DRT were about 800, 850, and 1, 400 bp long each and do not share any common restiction site. It is not clear yet if there exist any overlapping sequences among them. This partial cloning, however, was sufficient for the comparison of restriction maps with the other serotypes.

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Isolation of Infectious Pancreatic Necrosis Virus from Goldfish (Carassius auratus) and Chum Salmon (Oncorhynchus keta) in Korea. (금붕어(Carassius auratus)와 연어(Oncorhynchus keta)에서 췌장괴저 바이러스(IPNV)의 분리에 대하여)

  • Hah, Youg-Chil;Hong, Soon-Woo;Kim, Mi-Hee;Fryer, J.L.;Winton, J.R.
    • Korean Journal of Microbiology
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    • v.22 no.2
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    • pp.85-90
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    • 1984
  • Two viruses were isolated from kidney and spleen tissues of goldfish(Carasius auratus) and the ovarian fluid of chum salmon(Oncorhynchus keta). Both viruses replicated and produced cytopathic effect in EPC, CHSE-214, and CHH-1 cell lines at $15^{\circ}C$. The isolates were resistant to pH 3 and choloroform. Antiserum to infections pancreatic necrosis virus(IPNV) serotype VR 299 neutralized the infectivity of both of the isolates. Electron microscopy showed that the particles had typical IPNV particle morphology with average diameters of 55nm, This paper describes the first isolation of viruses infecting cultured fish in Korea.

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The Study on Information Visualization Methods Using 3D interactive Animation (3차원 인터랙티브 애니메이션을 활용한 정보시각화 방법에 관한 연구)

  • 김성곤
    • Archives of design research
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    • v.17 no.1
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    • pp.299-308
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    • 2004
  • The methods of presenting information are more variable with advanced computer media technology. However, when we visualize information of database, it is more difficult to understand data meaning by complex date, which is not required by user. To compose new information, user needs to combine own his memory and new information presenting result. To be quickly explored new information and be exactly understood relationship of data structure, the data presenting of visualized information is more structured and presented with meaning form. It is possible to make information visualization system, which users can explore database by oneself, with supporting interaction to be able to control and combine relationship of three-dimensional scene graphic structure, presenting factors of animation and data structure of database. There are several diagrams, such as VR diagram, Form structure diagram and simulated diagram as the case study.

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Detection of Birnavirus from Cultured Marine Fish Using Polymerase Chain Reaction (PCR) (중합효소연쇄반응법(Polymerase Chain Reaction, PCR)에 의한 남해안 양식산 어류로부터 Birnavirus의 검출)

  • Oh, Myung-Joo;Jung, Sung-Ju;Kim, Young-Jin
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.49-55
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    • 1999
  • To detect birnavirus from cultured marine fish, RT-PCR assay was developed. This method was specific for aquatic birnaviruses that include IPNV Sp., IPNV Ab, IPNV VR-299 and MBV Y6. The birnavirus gene was detected (birnavirus positive samples detected 46/50) from clinical samples signed with abdominal distension and overall darkening even though the samples gave negative results in virus isolation (birnavirus isolate with CHSE-214 cell showed 12/50).

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Studies on electrocardiogram of the normal Korean native cattle I. Standard limb leads (정상적(正常的)인 한우(韓牛)의 심전도(心電圖)에 관(關)한 연구(硏究) I. 표준지유도(標準肢誘導))

  • Choi, In-hyuk;Jung, In-sung;Kim, Nam-soo;Suh, Doo-seok
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.719-734
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    • 1993
  • The electrocardiographic(ECG) parameters on the standard limb leads in the normal Korean native cattle have been measured with a 3 channel Electrocardiograph built in a computed analysis. The study was conducted on the animals 98 heads of mean age of 17.7 months. Conduction parameters, waves, intervals and segments have been recorded. The recordings were analyzed as to shape and amplitude of the P and T waves and the components of the QRS complex. Heart rate was recorded by the Electrocardiogram which were a mean of $80.4{\pm}11.6beats/min$. And the younger had a higher heart rate than the older one. Average conduction times in the RP, the QRS complex and the QTc interval recorded $166.7{\pm}23.1msec.$, $79.7{\pm}8.8msec.$ and $395.5{\pm}30.4msec.$, in the P and T wave duration recorded $70.1{\pm}13.5msec.$ and $97.6{\pm}16.9msec.$, and in the PR and ST segment duration recorded $97.9{\pm}23.5msec.$ and $173.9{\pm}40.3msec.$, respectively. The wave forms in each lead observed various types. The amplitudes of wave type showed the highest frequency in each lead that were analyzed as follow : 1. In P wave, amplitudes of the positive type showed the frequency of 65.3%, 82.7% and 52.0% in leads I, II and III that were $103.1{\pm}47.8{\mu}V$, $115.2{\pm}37.3{\mu}V$ and $67.4{\pm}26.9{\mu}V$, and it showed the frequency of 54.1% and 85.7% in the leads aVL and aVF that were $63.7{\pm}23.0{\mu}V$, $88.0{\pm}83.6{\mu}V$, respectively. Average amplitude of the negative type showed the frequency of 78.6% in lead aVR which was $99.3{\pm}38.0{\mu}V$. 2. Average amplitude of the QRS complex were from $362.8{\pm}177.7{\mu}V$ to $532.8{\pm}253.9{\mu}V$(mean of $449.1{\pm}57.2{\mu}V$) that in all leads except lead I were manifested the Low-Voltage QRS complex(below 0.5mV). Average amplitudes of each wave type in the QRS complex aere $-50.2.4{\pm}258.2{\mu}V$ and $-428.6{\pm}195.1{\mu}V$ in the QS groups type that showed a frequency of 66.3%, 70.4% in the leads I and aVL, were $451.1{\pm}20.4.0{\mu}V$, $387.6{\pm}175.8{\mu}V$ and $299.3{\pm}146.5{\mu}V$ in the R groups type that showed a frequency of 48.0%, 53.1% and 34.7% in the leads III, aVR and aVF, and were $-307.5{\pm}180.3{\mu}V$, $201.4{\pm}77.2{\mu}V$ in the QR wave type which showed a frequency of 39.8% in lead II, respectively. 3. In T wave, amplitude of the positive type showed the frequency of 50.0%, 82.7%, 51.0% and 57.1% in leads II, III aVR and aVF which were $214.9{\pm}115.6{\mu}V$, $188.5{\pm}119.3{\mu}V$, $191.0{\pm}93.7{\mu}V$ and $165.7{\pm}91.9{\mu}V$, and the negative type showed a frequecny of 66.3% and 72.5% in leads I and aVL. that were $221.3{\pm}112.5{\mu}V$, $-173.6{\pm}86.7{\mu}V$, respectively. 4. Amplitude of ST segment in leads I, II and III were a mean of $-12.2{\pm}37.2{\mu}V$, $17.5{\pm}42.6{\mu}V$ and $28.3{\pm}40.4{\mu}V$, in leads aVR, aVL and aVF were $-3.9{\pm}32.5{\mu}V$, $-15.9{\pm}35.6{\mu}V$ and $26.2{\pm}37.5{\mu}V$, respectively.

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A Study on the Awareness and Preparation of the Forth Industrial Revolution of Some Health Department College Students (일부 보건계열학과 대학생의 4차 산업혁명 인식 및 준비도 연구)

  • Cho, Hye-Eun
    • Journal of the Korea Convergence Society
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    • v.11 no.12
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    • pp.291-299
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    • 2020
  • The purpose of this study was to be used as basic data for the development of future-type curriculum in health. The awareness and preparation of the forth industrial revolution were surveyed on 280 college students in health departments preparing medical technicians. A self-written structured questionnaire was used for data collection, and the recognition of the forth industry revolution was 2.74, 3D printing (3.59) was high, and neural network machine learning(2.33) was the lowest. Students majoring in Physiotherapy (3.00) had the highest perception, and those majored in Dental engineering(2.37) had the lowest perception, and there was a difference in the degree of perception of IoT by major (p=0.024). For the forth industrial revolution, 54.5% of students are preparing, and lack of interest (42.9%) is the most difficult reason to prepare, and 50.6% of educational experience and 60.9% of VR&AR game experience have experience. In the era of the forth industrial revolution, job loss (38.7%) was high, and the required competency was creative capacity (50.6%). Therefore, it is necessary to develop a curriculum related to the fourth industrial revolution and apply teaching methods that can increase the awareness and preparation of health college students in the era of the fourth industrial revolution.