• 대한수의학회지
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• 제48권3호
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• pp.287-293
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• 2008

The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.

• 한국어병학회지
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• 제22권3호
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• pp.211-218
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• 2009

경북 울진 지역에서 채집된 양식 강도다리와 충남 태안 및 부산 지역 넙치 시료로부터 분리한 MABV에 대한 유전자 비교를 위해 VP2-NSPhylogenetic VP3 region (432 bp)을 phylogenetic 분석에 이용했다. Sequence 확인 결과 MABV (08-KU)는 일본 방어에서 최초 분리 보고된 YTAV와 98%의 nucleotide 유사성이 나타났으며, 이전 보고된 다 른 여러 strain들과는 76%이상 유사한 것으로 확인되었다. 그리고 MABV (06-KP)와 MABV (08-KC)도 YTAV와 97-98%의 높은 sequence 유사성을 보였다. 또한 다양한 MABV strain들과의 비교를 위해 충남태안 및 부산지역 넙치 시료에서 분리한 MABV (08-KC)와 MABV (06-KP)에 대한 phylogenetic 분석도 실시하였다. 그 결과 분석에 사용된 MABV (08-KU0, MABV (06-KP), MABV (08-KC)는 모두 일본 방어에서 분리된 MABV Y6와 동일한 genogroup VII에 포함 되었다.

• 한국수산과학회지
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• 제47권5호
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• pp.556-561
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• 2014

We applied a combination of most probable number-polymerase chain reaction (MPN-PCR) methods using a PCR procedure targeting the H-NS (VP1133) gene to detect Vibrio parahaemolyticus presence and density in seawater as well as within short-necked clam Ruditapes philippinarum tissues collected from Gomso Bay, Korea. In 30 seawater samples, V. parahaemolyticus levels ranged from less than 1.8 to $1.1{\times}10^3MPN/100mL$, and samples from August showed higher than those from other months. Furthermore, the levels of V. parahaemolyticus in six short-necked clam samples ranged from $7.8{\times}10^2$ to $2.1{\times}10^3MPN/100g$, approximately 2.5 times higher than in seawater samples from the corresponding month. Our results provide data on V. parahaemolyticus contamination in seawater and short-necked clam tissues, and help to improve quantitative methods of assessing V. parahaemolytcius levels.

• 한국어병학회지
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• 제22권3호
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• pp.229-237
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• 2009

The causative agent for the tunic softness syndrome of the cultured ascidian Halocynthia roretzi from Jan 1999 to Feb 2009 was identified using virus isolation and polymerase chain reaction (PCR). The pathogenicity of the isolated virus MABV UR-1 strain was determined by experimental infection trials. The cytopathic effects was observed in CHSE-214 cell line at a level 5.1% (4/78) in normal ascidian and 1.8% in abnormal ascidian showing tunic softness syndrome signs. MABV gene was detected in 16.8% (18/107) of normal and 13.1% (5/38) of abnormal organisms by PCR. The ratio of MABV isolation and gene detection was similar level in normal and soft tunic diseased ascidian. Based on the VP2/NS junction region sequences, eight strains of virus isolated from ascidian, were included in the same genogroup with MABV which is originally isolated in wide ranges of marine fish and shellfish species. The UR-1 strain caused 60% mortality (36.5% mortality in control group) by immersion infection and 37% mortality (same mortality in control group) in injection infection indicating no significant differences in infected and control groups. These results suggest that ascidian can act as reservoir of the MABV, and this virus is not directly related with the ascidian mortality.