• 제목/요약/키워드: VP2

검색결과 642건 처리시간 0.03초

Effects of vacuum and high-oxygen modified atmosphere packaging on physico-chemical and microbiological properties of minced water buffalo meat

  • Jaberi, Rahimeh;Kaban, Guzin;Kaya, Mukerrem
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권3호
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    • pp.421-429
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    • 2019
  • Objective: In this study, the effects of vacuum (VP) and high-oxygen modified atmosphere ($80%\;O_2+20%\;CO_2$) packaging (HiOx-MAP) on physico-chemical and microbiological properties of minced water buffalo meat were investigated. Methods: After minced meat preparation, samples were packaged under VP and HiOx-MAP and stored at $2^{\circ}C{\pm}0.5^{\circ}C$ for 14 days. Samples taken on certain days were subjected to total aerobic mesophilic bacteria, total aerobic psychrotrophic bacteria, lactic acid bacteria, Pseudomonas, Enterobacteriaceae and yeast-mold counts as well as pH, color ($L^*$, $a^*$, and $b^*$) and thiobarbituric acid reactive substances (TBARS) analyses. Results: In minced water buffalo meat packaged under HiOx-MAP, TBARS value exceeded 1 mg malondialdehyde/kg on the 4th day of the storage. In VP samples, TBARS value remained close to initial TBARS value during storage. According to the findings, $a^*$ value was determined to be high in the HiOx-MAP samples within initial days of the storage. However, no significant changes in $a^*$ value were observed in VP samples during storage. In contrast, the mean value of $L^*$ was detected as higher in HiOx-MAP sample than VP samples. The count of psychrotrophic bacteria increased more than that of mesophilic bacteria during storage. The growth of Enterobacteriaceae and Pseudomonas was delayed in both the packaging methods. However, lactic acid bacteria exhibited more growth in VP samples compared to MAP samples. Conclusion: The lipid oxidation proceeded faster than expected in minced water buffalo meat packed with HiOx-MAP method. This situation adversely affected the $a^*$ value. On the other hand, similar microbiological results were obtained in both packing methods.

Influence of CBCT parameters on image quality and the diagnosis of vertical root fractures in teeth with metallic posts: an ex vivo study

  • Larissa Pereira Lagos de Melo;Polyane Mazucatto Queiroz;Larissa Moreira-Souza;Mariana Rocha Nadaes;Gustavo Machado Santaella;Matheus Lima Oliveira;Deborah Queiroz Freitas
    • Restorative Dentistry and Endodontics
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    • 제48권2호
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    • pp.16.1-16.11
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    • 2023
  • Objectives: The aim of this study was to evaluate the influence of peak kilovoltage (kVp) and a metal artifact reduction (MAR) tool on image quality and the diagnosis of vertical root fracture (VRF) in cone-beam computed tomography (CBCT). Materials and Methods: Twenty single-rooted human teeth filled with an intracanal metal post were divided into 2 groups: control (n = 10) and VRF (n = 10). Each tooth was placed into the socket of a dry mandible, and CBCT scans were acquired using a Picasso Trio varying the kVp (70, 80, 90, or 99), and the use of MAR (with or without). The examinations were assessed by 5 examiners for the diagnosis of VRF using a 5-point scale. A subjective evaluation of the expression of artifacts was done by comparing random axial images of the studied protocols. The results of the diagnoses were analyzed using 2-way analysis of variance and the Tukey post hoc test, the subjective evaluations were compared using the Friedman test, and intra-examiner reproducibility was evaluated using the weighted kappa test (α = 5%). Results: The kVp and MAR did not influence the diagnosis of VRF (p > 0.05). According to the subjective classification, the 99 kVp protocol with MAR demonstrated the least expression of artifacts, while the 70 kVp protocol without MAR led to the most artifacts. Conclusions: Protocols with higher kVp combined with MAR improved the image quality of CBCT examinations. However, those factors did not lead to an improvement in the diagnosis of VRF.

대장균에서 발현된 A군 로타바이러스 VP6 단백질을 이용한 로타바이러스 감염의 혈청학적 진단의 유용성 (Usefulness of Escherichia coli-expressed Recombinant VP6 Proteins of Group A Rotavirus in Serodiagosis of Rotavirus Infection)

  • 서지현;김소영;박지숙;임재영;박찬후;우향옥;윤희상;김원용;강형련;백승철;이우곤;조명제;이광호
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제13권2호
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    • pp.134-145
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    • 2010
  • 목 적: 로타바이러스 감염역학의 변화를 연구하기 위해 A군 로타바이러스의 VP6 유전자를 대장균에 발현시켜 확보한 rVP6 단백질이 항원성이 있는지를 확인하고 이것을 항원으로 한 효소면역측정법이 로타바이러스 IgG, IgA와 IgM 항체를 정량적으로 평가할 수 있는지 확인하고자 하였다. 방 법: 경상대학교병원에서 로타바이러스 감염을 진단받은 소아들 중 진단 받기 전, 진단 당시, 회복기 이후의 연속적인 혈청을 확보할 수 있었던 22명에게서 100개의 혈청을 경상대학교병원 인체자원은행으로부터 제공받아 로타바이러스 VP6 유전자를 클로닝 하여 대장균에 발현시켜 제조한 rVP6 항원으로 한 효소면역측정법으로 IgG, IgA와 IgM 항체 역가를 측정하였다. 이 중 건강한 신생아 4명에서는 면역 블로팅을 같이 시행하였다. 결 과: 건강한 신생아와 영유아 17명에서 감염 후 확보된 혈청에서 IgG, IgA, IgM 항체 중 최소한 한 종류의 항체 역가 증가가 동반되어 있었다. 면역이 저하된 소아 5명 중 4명에서는 IgG 항체 역가는 증가되었으나 IgA 항체 역가는 2명에서만 증가하였고, IgM 항체 역가는 5명 모두 증가하지 않았다. 신생아 4명에서 시행된 면역 블로팅 검사에서는 IgM 항체인 경우는 효소면역측정법보다 예민하게 진단 초기부터 4명 모두 양성으로 판정되었다. 결 론: A군 로타바이러스의 VP6 유전자를 대장균에 발현시켜 확보한 rVP6 단백질은 항원성이 있으며 이것을 항원으로 한 효소면역측정법은 로타바이러스 감염후 IgG, IgA, IgM 항체 역가 증가를 정량적으로 평가할 수 있어 지역사회에서 발생한 로타바이러스 감염역학의 변화를 연구하는데 유용할 것으로 판단된다.

기능성 프탈로시아닌 물질의 제조 및 대전방지/UV 차단 특성 (Preparations and Antistatic/UV Blocking Properties of Dual Functional Phthalocyanine Materials)

  • 강영구;임대우;김시석;박병기
    • 한국안전학회지
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    • 제23권1호
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    • pp.66-71
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    • 2008
  • $PcFe(4-VP)_2$[Pc: Phthalocyanine, 4-VP: 4-Vinylpyridine]와 $PcFe(VIM)_2$[VIM: 1-Vinylimidazole]의 제조를 재래식 방법인 유기용제 사용에 따른 폭발 및 유해증기 발생 위험성을 차단하기 위해 무용제 방식으로 ligand인 4-VP와 VIM을 반응매체로 하여 PcFe를 밀폐용기에서 용융시키는 새로운 방법을 도입, 제조하여 분말상 진청록색의 물질을 제조하였다. 이들 물질은 $CH_2Cl_2$에 가용성인 $PeFeL_2$형태의 물질이다. 제조된 물질을 ATR-FTIR, UV/Vis, DSC/TGA, Surface Resistivity Meter를 이용하여 분석한 결과 film 상태에서 UV/Vis 차단 특성이 뛰어남과 동시에 대전 방지특성을 나타내며 또한 내열성이 우수한 물질이 제조되어 사용 및 응용이 가능할 것으로 사료된다.

흉부(胸部) 및 복부(腹部)X선촬영시(線撮影時) 환자(患者)의 골수선량(骨髓線量)에 대한 연구(硏究) (A Study of the Bone Marrow Dose in Chest and Abdomen Radiography)

  • 최종학;허준
    • 대한방사선기술학회지:방사선기술과학
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    • 제13권2호
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    • pp.31-36
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    • 1990
  • We got the following results from the experiment and examination in order to measure the bone-marrow dose of the patients when we did chest or abdomen radiography in the hospitals located in Seoul City from Jan. 1989 until Feb. 1990. 1. In the exposure factors for chest radiography, tube voltage $60{\sim}69\;kVp$ took 48.3%, $80{\sim}89\;or\;90{\sim}99\;kVp$ took 13.8% respectively, $70{\sim}79\;kVp$ 10.3% and $100{\sim}129\;kVp$ 10.3%. In tube current and exposure times, $6{\sim}10\;mAs$ took 41.4%, $16{\sim}20\;mAs$ took 20.7% and $11{\sim}15\;mAs$ 13.8%, measure under 5mAs 10.4% orderly. 2. In chest radiography, the bone-marrow dose came to the minimum 3.48 mrad, to the maximum 35.67 mrad, to the mean 14.46 mrad, to the standard deviation 8.89 mrad. 3. Comparing bone-marrow doses of the patients when we used Bucky technique and non-Bucky technique, that of Bucky technique was very higher than that of non-Bucky technique. Because the result was that Bucky technique had the span of $6.09{\sim}35.67$ mrad, while non-Bucky technique had the span of $3.48{\sim}17.40$ mrad. 4. In the exposure factors for abdomen radiography, tube voltage of $70{\sim}79\;kVp$ was 63.0%, that of $80{\sim}89\;kVp$ was 22.2%, that of $60{\sim}69\;kVp$ was 11.1 %. Tube current and exposure times of $31{\sim}40\;kVp$ was 33.4%, that of $51{\sim}60\;mAs$ was 29.6% and that of $41{\sim}50\;mAs$ was 22.2%. 5. In abdomen radiography, the bone-marrow dose of the patients came to the minimum of 6.96 mrad, to the maximum of 60.90 mrad, to the mean of 35.73mrad, to the standard deviation of 12.65 mrad.

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관전압과 관전류량이 노출 지수에 미치는 영향 : 원뿔형 피라미드 팬텀 방사선영상 중심으로 (Effects of Tube Voltage and Tube Current on Exposure Index : Focused on Radiographic Images of Cone Pyramid Phantom)

  • 성열훈
    • 한국방사선학회논문지
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    • 제13권5호
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    • pp.749-755
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    • 2019
  • 본 연구에서는 관전압과 관전류량이 디지털 방사선영상에서 적용되고 있는 노출 지수에 미치는 영향을 알아보고자 하였다. 방사선발생장치는 인버터방식의 디지털 X선 발생장치를 사용했으며 영상검출체는 포터블 형태의 무선 디텍터를 그리드 없이 사용하였다. 방사선영상은 3D 프린터를 이용하여 제작한 원뿔형 피라미드 팬텀을 이용하여 획득하였다. X선의 관전압 조사조건은 40 kVp부터 120 kVp까지 10 kVp 씩 증가시켰고 각 관전압에서 관전류량은 1 mAs에서부터 128 mAs까지 배수적으로 증가시켰다. 그 결과 관전압이 EI와 높은 $R^2$ 값으로 로그 함수적 관계가 있었으며 관전류량이 매우 높은 선형적인 관계가 있었다. 또한 영상 검출체의 면적선량과 EI도 $R^2$ 값이 0.76 이상으로 높은 상관관계가 있었다. 결론적으로 관전류량이 EI에 선형적으로 영향을 주었으며, 적절한 영상품질 유지를 위해서는 예측이 용이한 관전류량을 주로 조절하는 것이 유리하다고 판단된다.

Targeted Delivery of VP1 Antigen of Foot-and-mouth Disease Virus to M Cells Enhances the Antigen-specific Systemic and Mucosal Immune Response

  • Kim, Sae-Hae;Lee, Ha-Yan;Jang, Yong-Suk
    • IMMUNE NETWORK
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    • 제13권4호
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    • pp.157-162
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    • 2013
  • Application of vaccine materials through oral mucosal route confers great economical advantage in animal farming industry due to much less vaccination cost compared with that of injection-based vaccination. In particular, oral administration of recombinant protein antigen against foot-and- mouth disease virus (FMDV) is an ideal strategy because it is safe from FMDV transmission during vaccine production and can induce antigen-specific immune response in mucosal compartments, where FMDV infection has been initiated, which is hardly achievable through parenteral immunization. Given that effective delivery of vaccine materials into immune inductive sites is prerequisite for effective oral mucosal vaccination, M cell-targeting strategy is crucial in successful vaccination since M cells are main gateway for luminal antigen influx into mucosal lymphoid tissue. Here, we applied previously identified M cell-targeting ligand Co1 to VP1 of FMDV in order to test the possible oral mucosal vaccination against FMDV infection. M cell-targeting ligand Co1-conjugated VP1 interacted efficiently with M cells of Peyer's patch. In addition, oral administration of ligand-conjugated VP1 enhanced the induction of VP1-specific IgG and IgA responses in systemic and mucosal compartments, respectively, in comparison with those from oral administration of VP1 alone. In addition, the enhanced VP1-specific immune response was found to be due to antigen-specific Th2-type cytokine production. Collectively, it is suggested that the M cell-targeting strategy could be applied to develop efficient oral mucosal vaccine against FMDV infection.

환자에게 주는 선량을 감소시켜주는 니오비움 필터의 대체물로서의 저렴한 알루미늄 필터 (Aluminum Equivalent Filter As an Inexpensive Alternative to the Niobium Filter in Reducing Patient Dose)

  • Kim, Chang-Sean
    • 한국의학물리학회지:의학물리
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    • 제6권2호
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    • pp.3-12
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    • 1995
  • 두께가 50 $\mu\textrm{m}$인 니오비움 필터와 정량적으로 대등하게 결정된 알루미늄 필터를 입사표면 선량, 영상의 질, X-선관에 걸리는 부하를 진단 방사선의 에너지 영역에서 평가하였다. 입사 표면 선량의 감쇠와 선관에 걸리는 부하의 증가를 비교해 볼 때 두 경우에 특이할 만한 큰 차이가 없었고 이 때 필름상의 화상의 대조도는 일정하게 유지 시켰다. 임상에서 알루미늄 필터를 니오비움 필터로 대용하기 위하여 진단 방사선의 에너지 영역의 중간지점, 90 kVp, 에서 대등한 알루미늄 필터의 두께를 측정하였고 이 필터를 73 kVp 와 125 kVp에 적용시켜 입사 표면 선량과 X-선관에 걸리는 부하를 비교 평가하였다. 선택된 두 kVp에서 두 필터의 경우에 특이할 만한 차이는 없었다. 니오비움과 대등한 두께의 알루미늄 필터는 니오비움 필터의 저렴한 대체물로 쓰일 수 있다.

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Effects of Individual Sweating Response on Changes in Skin Blood Flow and Temperature Induced by Heat of Sorption Wearing Cotton Ensemble

  • Tanaka, Kaori;Hirata, Kozo
    • 한국의류산업학회지
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    • 제2권5호
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    • pp.398-404
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    • 2000
  • We examined the effect of individual sweating responses on thermoregulatory responses induced by heat of sorption, immediately after the onset of sweating. The present study consists of two experiments. In experiment 1, made of 100% cotton (C) and 100% polyester (P) clothing were exposed in the chamber at ambient temperature (Ta) of $27.2^{\circ}C$ and relative humidity (rh) raised from 50% to 95% at five different increase rates of environmental vapor pressure (VP). The increase rate of clothing surface temperature (Tcs), peak Tcs and peak time showed significant correlation with the increase rate of environmental VP in C-clothing (p<0.05). In experiment 2, seven female subjects were studied during leg water immersion ($35-41^{\circ}C$) for 70min in Ta of 27.2 and 50%rh. There were significant positive correlations in the increase rate of clothing microclimate VP vs. changes in Tcs, skin blood flow, mean skin temperature and mean body temperature (p<0.05). The present results showed that individual clothing microclimate VP had significant effects on thermoregulatory responses induced by heat of sorption wearing C ensembles.

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Videodensitometry법을 이용한 X선사진의 흑화도와 대조도에 관한 연구 (THE VIDEODENSITOMETRIC ANALYSIS OF THE RADIOGRAPHIC DENSITY AND CONTRAST)

  • 유영선;이상래
    • 치과방사선
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    • 제22권2호
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    • pp.293-303
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    • 1992
  • Generally the patient's absorb dose and readability of radiograms are affected by the exposure time and kVp of which are related with the radiographic density and contrast The investigator carried studies to know the adequate level of exposure time and kVp to obtain the better readability of radiograms. In these studies dried human mandible with selfcuring acrylic resins attached with aluminum step wedge was used and readability of radiograms were compared with each other by videodensitometry among various combination sets of the exposure time, such as 5, 6, 8, 12, 15, 19, 24, 30, 38, 48 and 60, and varing level of kVp, such as 60, 65, 70, 80 and 90 respectively. The obtained results were as follows: 1. As exposure time and kVp were increased, radiographic density of radiograms was increased. 2. The subject contrast was increased where aluminum step wedge was thin and reduced in the reversed condition. At the thin aluminum step wedge, subject contrast was increased at the condition of lower kilovoltage than that of higher kilovoltage. 3. In the case of non-constant radiographic density, the radiographic contrast was reduced with the increment kilovoltage. The radiographic contrast was increased in the lower kilovoltage with the longer exposure time and the higher kilovoltage with the shorter exposure time. 4. At the condition of short exposure time, better readability of each reading item was obtained with the increment of the kilovoltage but at the opposite condition increasing exposure time worsened readability of radiograms. Since X-ray machine in the current dental clinics is fixed between the range of 60-70kVp and 10mA, good radiograms can be obtained by varied exposure time. But according to the conclusion of these studies, better radiograms can be obtained by using filtered high kVp and then the absorb dose to patient and exposure time can be reduced.

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