• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.17 no.1
• /
• pp.638-645
• /
• 2016

The purpose of this study was to develop land use planning methods for carbon reduction of transportation and verify the effectiveness. Therefore, this study derived carbon reduction design elements, such as high-density suburb, mixed-use development, pedestrian network and community corridor, which can be applied in the land-use planning stage by examining previous research. The carbon reduction design elements utilized the actual site during the research process. The carbon reductions were estimated using VISUM. Consequently, when carbon reduction design is applied to the site, the carbon emissions declined in the year. As a result of estimating the carbon reduction, approximately 450.7tCO2/yr was reduced.

• KSBB Journal
• /
• v.14 no.5
• /
• pp.578-584
• /
• 1999

The lectin was purified through 0.15 M NaCl extraction, ammonium sulfate precipitation, sepharose 4B affinity chromatography and gel filtration using sephadex G-150 from the leaves of Visum album collected in Mt. Duk Yu. The final gel filtration step resulted in 11.64 folds purification with 0.14% of recovery yield. We also performed biochemical characterization of the purified Visum album lectin. HPLC analysis of lectin purified by gel filtration revealed a singel peak. The analysis of the purified lectin by SDS-PAGE showed a tetramer composed of two identical subunits with molecular weights of 32 and 30 kDa. The lectin was a glycoprotein containing 14.4% carbohydrate, which consist of glucose, fructose, arabinose and xylose, and the amino acids such as phenylalanine, lysine and tyrosine. The purified lectin agglutinated human red blood cell types with similar potency, but when tested against red blood cells from mouse, bovine, rabbit, chicken and porcine, significant difference in potency were observed. Hemaggluting activity was inhibited by D-galactose, D-mannose, D-lactose and D-raffinose, but not by D-glucose, D-glucosamine, D-mannosamine, L-fructose, D-xylose, D-arabinose, D-galacturonic acid, D-fructose, L-rhamnose and N-acetyl-D-galactosamine. The optimal pH and thermal stability of the purified lectin were pH 4.0-7.0 and 20-5$0^{\circ}C$, respectively.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.16 no.1
• /
• pp.78-89
• /
• 2017

This study derived the algorithm of a public transportation route search that adds safety and environmental costs according to user preference. As the means of an algorithm application and evaluation, Macro Simulation, VISUM was conducted for an analysis. The route using the subway, which is relatively low in safety and environment resistance value was preferred, and it was analyzed to select the safe and environmental route even though it detours. This study can be applicable when to verify the algorithm of route search considering safety and environment, and when introducing the algorithm of route search according to user preference in the smart-phone application in the future, it can provide users with very useful information by choosing a route as for safety and environment, and through this, the quality of user-friendly information provision can be promoted.