• 제목/요약/키워드: VEGETATIVE PROPAGATION

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노각나무 천연집단의 특성 및 성숙목의 맹아지 삽목증식 (Vegetative Propagation of Stewartia koreana through Cuttings of Mature Sprouts)

  • 김기철;이성규;이갑연
    • 한국농림기상학회:학술대회논문집
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    • 한국농림기상학회 2001년도 춘계 학술발표논문집
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    • pp.85-88
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    • 2001
  • 차나무과에 속하는 노각나무(Stewartia Koreana)는 내음성이 강하고 공해에 잘견디는 수종으로서 6월말부터 8월에 걸쳐 흰색의 동백꽃 모양의 꽃이 피고, 수피의 모양이 모과나무의 수피와 비슷한 홍황색의 광택이 나는 비단과 같은 얼룩무늬 수피를 가지고 있어 멀리서 보아도 눈에 띄일 정도로 아름다운 모습을 지녔으며, 가을에는 단풍의 아름다움도 커 조경 및 원예적 가치가 높은 수종이다.(중략)

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야생딱총나무(Sambucus williamsii var. coreana Nakai)의 조경식목 화를 위한 기초 연구 (Studies on the Sambucus silliamsii var. coreana Nakai for Landscape use)

  • 김정미;박용진;이기훤
    • 한국조경학회지
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    • 제21권1호
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    • pp.139-148
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    • 1993
  • The present experiments have been conducted to find out the plant's growth environments habitate, mode of life, characteristic of photosynthesis, habit of growth and propagation. The results of this study are as follows: 1. Sambucus williamsii var. Coreana distributes around all sides of native site without having any relation to altitude, inclination and direction. 2. In the native site, Robinia pseudo - acacia and its neighboring species were Prunus yedoensis, Acer pseudo-sieboldianum, Lindera obtusiloba and Staphylea bumalda. From 21 to 41 types of species were located in the vegetation of the quadrat area. 3. According to the variation of leaf temperature with the result of the change of net photosynthetic rate, the optimum temperature for growth is $25^{\circ}C$. 4. The rooted rate of vegetative propagation was the highest at 100ppm IBA plot and the lowest at 200ppm BA plot. 5. The rooted rates of Sambucus williamsii var. coreana in perlite 50% bed, vermiculite bed and peatmoss 50%+sand 50% bed are higher than others.

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In vitro Formation of Tuberous Roots from Root Ends in the Rooted Tuberous stem without shoots in Cyclamen persicum MILL.

  • Lim, Jong-Gu;Junzo, Fujigaki
    • Plant Resources
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    • 제7권3호
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    • pp.222-225
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    • 2004
  • In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

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知母의 生育特性과 部位別 Sarsasapogenin 含量 (Growth Characteristics and Content of Sarsasapogenin in Different Plant Parts of Anemarrhena asphodeloides Bunge)

  • 허일범
    • 한국자원식물학회지
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    • 제10권2호
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    • pp.183-187
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    • 1997
  • 본 실험은 지모의 생리, 생태적 특성과 성숙한 상태의 각 부위별 Sarsasapogenin의 함량을 비교 분석하고자, 5개의 수집종에 대해 1993년부터 '95년까지 충남농촌진흥원 특작포장에 재배하고 성숙된 지모의 각 부위별 Sarsasapogenin 함량을 T.L.C plate 방법에 의해 분석한 결과 다음과 같은 결론을 얻었다. 1. 5개 수집종 중 주로 종실(種實)형을 나타낸 것은 공주, 청양 수집종이고 영양번식(營養繁殖)형은 금산, 연기, 유성종에서 주로 분류되어 있으며, 종실(種實)형이 비종실형보다 생육(生育) 및 형태적(形態的) 특징(特徵)이 양호하였다. 2. BuOH에 엑스를 산(酸)으로 가수분해(加水分解)한 후 T.L.C 용매(溶媒) 전개조건(展開條件)인 $CHCl_3$ : MEK : EtOH의 비율을 11 : 2 : 0.5로 하여 sarsasapogenin 분리(分離)가 가능하였고 Dual wave length T.L.C scanner로 정량(定量)이 가능(可能)하였다. 3. 주근(主根)과 세근(細根)의 sarsasapogenin의 량(量)은 각각(各各) 1.67mg/10g, 1.31mg/10g으로 세근(細根)에 sarsasapogenin의 량(量)이 주근(主根)보다 1.1배(倍)로 높게 나타나 세근(細根)에 sarsasapogenin 계통(系統)의 물질(物質)이 가장 많음을 알 수 있었다. 그러나 지상부(地上部)의 sarsasapogenin 함량은 미미한 것으로 나타났다.

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Composition of Secondary Metabolites in Various Parts of 'Seolhyang' Strawberry Plants

  • Kim, Dong Sub;Na, Haeyoung;Kwack, Yurina;Kim, Sung Kyeom;Heo, Jeong Wook;Chun, Changhoo
    • 원예과학기술지
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    • 제31권2호
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    • pp.224-230
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    • 2013
  • The objective of this study was to identify the content of phenolic and volatile organic compounds in edible and non-edible parts of 'Seolhyang' strawberry plants. We performed a comparative chemical analysis of the compounds found in roots, leaves, petioles, runners, and unripe and ripe fruits during vegetative propagation and reproductive growth. The contents of ellagic and gallic acids in the leaves of runner plants during vegetative propagation were $7.36{\pm}1.10$ and $5.07{\pm}3.66mg{\cdot}g^{-1}$ FW, respectively, and were higher than those in the other parts. The main volatile organic compound was identified as 3-hexen-1-ol, and it was mostly detected in leaves. The content of ellagic acid in leaves during reproductive growth was $12.96{\pm}2.30mg{\cdot}g^{-1}$ FW, while that in the other parts was below $6.00mg{\cdot}g^{-1}$ FW. The content of gallic acid in unripe fruits was $2.75{\pm}0.48mg{\cdot}g^{-1}$ FW and was higher than that in the other parts. Ripe fruits contained the lowest contents of ellagic and gallic acids but contained the most diverse volatile organic compounds, including sesquiterpenes, among the tested plant parts. The results indicate that non-edible parts (e.g., leaves and unripe fruits) of strawberry plants can be used as a raw material for antioxidant and anti-inflammatory agents, and edible parts (i.e., ripe fruits) can be available for making an essential oil.

Mycelial Propagation and Molecular Phylogenetic Relationships of Commercially Cultivated Agrocybe cylindracea based on ITS Sequences and RAPD

  • Alam, Nuhu;Kim, Jeong-Hwa;Shim, Mi-Ja;Lee, U-Youn;Lee, Tae-Soo
    • Mycobiology
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    • 제38권2호
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    • pp.89-96
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    • 2010
  • This study evaluated the optimal vegetative growth conditions and molecular phylogenetic relationships of eleven strains of Agrocybe cylindracea collected from different ecological regions of Korea, China and Taiwan. The optimal temperature and pH for mycelial growth were observed at $25^{\circ}C$ and 6. Potato dextrose agar and Hennerberg were the favorable media for vegetative growth, whereas glucose tryptone was unfavorable. Dextrin, maltose, and fructose were the most effective carbon sources. The most suitable nitrogen sources were arginine and glycine, whereas methionine, alanine, histidine, and urea were least effective for the mycelial propagation of A. cylindracea. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR. The sequence of ITS2 was more variable than that of ITS1, while the 5.8S sequences were identical. The reciprocal homologies of the ITS sequences ranged from 98 to 100%. The strains were also analyzed by random amplification of polymorphic DNA (RAPD) using 20 arbitrary primers. Fifteen primers efficiently amplified the genomic DNA. The average number of polymorphic bands observed per primer was 3.8. The numbers of amplified bands varied based on the primers and strains, with polymorphic fragments ranging from 0.1 to 2.9 kb. The results of RAPD analysis were similar to the ITS region sequences. The results revealed that RAPD and ITS techniques were well suited for detecting the genetic diversity of all A. cylindracea strains tested.

상자파종에 의한 규격화된 잔디의 생산 (Standardized Sod Production Using Box Seeding)

  • 구자형;김태일;전대우;최종명
    • 아시안잔디학회지
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    • 제9권3호
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    • pp.187-198
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    • 1995
  • The objective of this research was to produce sod by box seeding for zoysiagrass or by vegetative propagation for zoysiagrass and manilagrass.1 Various ratio of peatmoss to sand(v /v) were prepared to find idea[ medium for fast and light weight sod production. Then, the days required for sod formation, the effect of growth regulators on the growth of turfgrass, and the various storage methods for winter keeping of sods were also investigated. 1.The mixed medium of sand and peatmoss(v /v, 1 : 2) showed more biomass production than that of sand. 2.In comparison of seeding rate of zoysiagrass, the amount of log /$m^2$ was most effective in the fast and dense sod formation. The amount of 20g /$m^2$ also showed fast sod formation. But, it resulted in weak plant and less tillering. During April to June, about 100 days were required to form sod with seeding rate of 5g /$m^2$ regardless of seeding time. Whereas 80 days were required to form sod in the rate log /$m^2$, which was 20 days shorter than that of 5g /$m^2$. 3.More than 85% of shoots in sod stored in field or plastic house during the winter time resumed the growth in good appearance after transplanting. The whole covering of ground with sod resulted in less weeds and faster formation of lawn. 4.Vegetative propagation of manilagrass showed about 7 to 15 days faster formation of sod than that of zoysiagrass. Application of GA increased shoot growth and BA increased the total number of tillering. However, the effects of the combined application of GA and BA were negligable.

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토천궁(土川芎)의 줄기삽목에 의한 대양번식(大量繁殖) (Mass Propagation by Stem Cutting in Ligusticum chuangxiong Hort.)

  • 유홍섭;방진기;김영국;이봉호
    • 한국약용작물학회지
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    • 제7권3호
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    • pp.200-204
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    • 1999
  • 토천궁의 줄기마디를 이용한 효율적인 삽목번식 기술을 개발하고자 수행한 시험결과를 요약하면 다음과 같다. 1. 삽목시는 8월30일 $1{\sim}2$마디삽목에서 발근율이 81%로 가장 높았고 발근상태도 양호하였다. 2. 삽수채취에 따른 수량감소는 주당경수의 50%채취에서 9%이하 였으나 100% 채취에서는 $10{\sim}23%$로 비교적 높았다 3. 삽목묘의 포장정식후 활착율은 9월25일 및 10월25일 정식에서 각각 85%, 87%로 높았으나 11월25일 정식에서는 28%로 낮았다. 4. 10a당 수량은 9월25일및, 10월25일 정식에서 각각 127kg, 120kg으로 높았다.

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Vegetative Propagation of Amaryllis (Hippeastrum × johnsonii ) by Different Cutting Methods

  • Kharrazi, Mahdiyeh;Tehranifar, Ali;Nemati, Hossein;Bagheri, Abdol-Reza
    • 원예과학기술지
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    • 제35권3호
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    • pp.373-380
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    • 2017
  • Amaryllis ($Hippeastrum{\times}johnsonii$) is commonly propagated by three methods: seeds, offset bulblets, and twin scaling. Since the number and size of bulblets produced by these methods are low, we conducted an experiment to evaluate different bulb cutting methods for propagation. For this purpose, bulbs (circumference of 30 - 35 cm) were sectioned or notched into 8, 12, or 16 segments or twin-scaled into 48, 72, or 96 segments for bulblet formation. Our results show that the largest number of bulblets was produced by the twin scaling method, but they take longer to grow to a final, commercial size. Sectioning and notching resulted in larger, but fewer bulblets than twin-scaling. Compared to notching, sectioning provided more space for the bulblets to grow, and is therefore the recommended method. While increasing the number of sections cut from a single bulb resulted in a larger number of bulblets, the diameter of the bulblets decreased. Therefore, sectioning the bulb into 8 segments was the best method for producing an acceptable number of vigorous bulblets.

Monosiphonous growth and cell-death in an unusual Bostrychia (Rhodomelaceae, Rhodophyta): B. anomala sp. nov.

  • West, John A.;Loiseaux de Goer, Susan;Zuccarello, Giuseppe C.
    • ALGAE
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    • 제28권2호
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    • pp.161-171
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    • 2013
  • A morphologically distinct lineage within the Bostrychia moritziana-B. radicans species complex is described as a new species. Bostrychia anomala has thalli with branched monosiphonous filaments with apical cell divisions. The species has terminal tetrasporangial stichidia, each subtending cell bearing tetrasporangia with 2 cover cells. Discharged spores divide transversely, the lower cell first forming a narrow rhizoid and the upper cell forming a monosiphonous shoot. Females have subterminal procarps and males have terminal spermatangial stichidia. Carposporophytes are spherical. Isolates in culture show a pattern of cell death not associated with injury, reminiscent of programmed cell death. Bostrychia anomola shows cell death at intervals along the filaments resulting in division of adjacent cells on either side of the dead cell re-joining the filament; cell division of only one adjacent cell resulting in branching at that site; or filaments fragmenting at the cell death point with adjacent cells forming new apical cells, a means of thallus propagation. The cell death pattern could be a method of filament propagation in the mangrove environment where sexual reproduction is rare.