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Preparation of fermentation broth of Sparassis latifolia containing soluble β-glucan using four Lactobacillus species (수용성 β-glucan을 함유한 꽃송이버섯 발효액의 제조)

  • Jo, Han-Gyo;Choi, Moon-Hee;Shin, Hyun-Jae
    • Journal of Mushroom
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    • v.13 no.1
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    • pp.50-55
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    • 2015
  • Glucan has been shown to have a significant role in the activation of the immune system, including increased activity of macrophages and so on. Sparassis latifolia (formerly S. crispa) is an edible mushroom abundant in dietary fiber and widely known to contain high levels of ${\beta}$-glucan. In the present study, fermentation broths containing soluble ${\beta}$-glucan were prepared by fermentation with mushrooms with four Lactobacillus species (L. plantarum subsp. Plantarum, L. acidophilus, L. helveticus, and L. delbrueckii subsp. Bulgaricus). After culturing four Lactobacillus spp. in MRS broth, each Lactobacillus was inoculated into MRS broth containing S. latifolia powder 5% (w/v) at $37^{\circ}C$ in an anaerobic incubator for five days. It showed that the ${\beta}$-glucan contents were different in each fermentation sample. The suitable conditions for the preparation of mushroom fermentation broths were investigated and discussed.

Effects of dietary Alisma canaliculatum(Alismatis rhizoma), Viscum album (Mistletoe) and Cornus officinalis (Corni fructus) probiotics as feed additives on growth performance and immunity in growing pigs (사료내 택사, 겨우살이 및 산수유 생균제 첨가가 비육돈의 생산성 및 면역성에 미치는 영향)

  • Kim, Ki-Soo;Kim, Gwi-Man;Ji, Hoon;Park, Sung-Wook;Yang, Jeong-Seung;Yang, Chul-Ju
    • Korean Journal of Veterinary Service
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    • v.33 no.4
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    • pp.375-385
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    • 2010
  • An investigation was conducted to evaluate the effects of supplementing Alisma canaliculatum, Viscum album and Cornus officinalis probiotics on the growth performance and immune response in growing pigs. This experiment was conducted using 120 pigs (crossing of Landrace${\times}$Yorkshire and castrated) which were assigned to 5 treatments in 3 replications with 8 pigs per replications. The dietary treatments were NC group (without antibiotics), PC group (basal+Oxytetracycline 50ppm), AC group (basal+A. canaliculatum 0.5%), VA group (basal+V. album 0.5%) and COP group (basal+C. officinalis probiotics 0.5%). The initial body weights of pigs were 35kg on average and the experiment lasted for 9 weeks. The experimental animals were kept in the pens following a completely randomized design. They were provided the diets adequate for grower stage as recommended by NRC (ME:3,265 kcal/kg and CP:16%). COP fed pigs showed lower weight gain up to 6 weeks of age compared to NC group and other groups without significant differences (P>0.05). The carcass weights of pigs fed VA and COP were significantly higher compared to NC group (P<0.05), Back fat thicknesses groups fed three different additives were higher than NC group and lower then PC group (P<0.05). Crude fat contents in loin meat were significantly lower in groups fed three different additives while moisture contents of those three groups were higher than other groups (P<0.05). The thiobarbituric acid reaction substance (TBARS) value measured at fresh and $2^{nd}$ weeks was lower in additives fed groups but no statistical differences were observed among the treatments (P>0.05). Significantly highest PUFA (16.42g/100g) and ${\omega}$-3 fatty acids (ALA, EPA and DHA) content of meat were observed in COP fed pigs compared to NC group (P>0.05), which might mean that three additives function to enhance serum IgG in pigs. In consequence, it can be suggested that AC, VA and COP may have a potential to replace antibiotics as growth promoter and immune enhancer in the diets for growing pigs.

A Study on the effects of Ginseng Radix extract on rat with nephrotoxicity (인삼(人蔘)이 Cyclosporin A로 유발(誘發)된 백서(白鼠)의 신손상(腎損傷)에 미치는 영향(影響))

  • Seo, Ho-Suk;Han, Yang-Hi;Park, Jong-Hyung;Jun, Chan-Yong;Kim, Dong-U;Park, Se-Gi;Lee, Chung-Jung-Hye;Go, Jae-Chul;Choi, You-Kyung;Baek, Eun-Gi;Hong, Ui-Sil;Park, Ji-Yun;Go, Seung-Hi
    • The Journal of Internal Korean Medicine
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    • v.22 no.1
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    • pp.45-52
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    • 2001
  • Purpose : This study is to find out the Ginseng Radix extract on rats nephrotoxicity when the rates were induced by Cs A. Methods : The experimental animals were divided into 3 groups and treated as follows: nothing was given to Sample A, Sample B was given normal saline after an I.V. injection of Cs A, and Sample C was given Ginseng Radix extract after a Cs A injection. The groups were observed for 14 days. After the prescription of the medicines, the following levels were tested: serum BUN, creatinine, total protein, sodium, potassium, and chloride ions. Results : 1. Changes in serum level. The levels of GPT, GOT, and BUN were significantly reduced in the experimental group when compared with those of the control group. The total protein level showed significant elevation when compared with those of the control group. The chloride level in the serum in the sample group was insignificantly reduced. The creatinine level was insignificantly increased. The Potassium level decreased, mildly. The Sodium and The Potassium levels in the serum in the sample group showed insignificantly lower levels than those of the control group. 2. Changes in the urine level. Urine specific gravity showed a significant increase, on the 14th day, in the experimental group when compared with that of the control group. The urinary ceatinine levels showed and insignificant increase, followed by an insignificant decrease. Conclusion : It can be inferred that Ginseng Radix may improve nephrotoxicity and hepatoxicity in rats when induced by Cyclosporin A.

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A Study on Men's Costumes of the Parthian Period in Persia (페르시아 파르티아[Parthia]왕조 남자복식 연구)

  • YiChang Young-Soo
    • Journal of the Korean Society of Costume
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    • v.56 no.4 s.103
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    • pp.65-81
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    • 2006
  • This is the study on the costumes of Parthian Period in Persia which had powerful influences on the Occidental and Western cultures in B.C 3 to A.D 3. With different types of Parthian costumes in each period, this study shows what types of costumes people used to wear and how they were co-related with its surrounding cultures. The ancient costumes of Korea had something to do with the elements of the Northern part at that time. To make an intensive study of Korean ancient costumes, these Parthian costumes which are known to those of northern nomadic tribes are studied to provide basis elements for study between the Korean styles and the exotic styles. The basic forms of the Parthian styles were jackets and trousers. They consisted of three kinds of jackets such as those to be adjusted In the front, tunics and coats. As for trousers, they were divided into three types such as those with horizontal pleats, round-side pleats and vertical pleats. 1) The jacket to be adjusted in the front was long enough to reach the hip and fit the body reasonably tight, and its neckline was shaped into V-type. It was a very popular style among the people of high and low classes. 2) The tunics had narrow sleeves. It was a one-piece pattern which reached the knees. It was settled with a belt on the waist. Its neckline was shaped into the round but its slit was not cleat. Its styles were into tight-fit and drapery ones. 3) The coats were almost similar to the jacket to be adjusted in the front with short length, but they were entirely long and open. They appeared later than the short jackets and the people of high class seemed to wear these styles. 4) 1'rousers with horizontal pleats - They had almost horizontal pleats on them, and they were a little tight. They seemed to be the early style in the Parthian period. 5) Trousers with round side-pleats - With saggy side pleats on them, these trousers had round pleats like a drapery style. This pattern was also considered Hellenistic elements shown in the Parthian costumes. The trousers consisted of the underdrawers and the leggings (called salwar or shalwar), which is thought to emphasize its functionality for its wearer to mount a horse with more ease. 6) Trousers with vertical pleats - With straight vertical pleats on them, these trousers had some volumes and bias decorations in the middle of them. These styles were thought to be worn by the ruling class of the Parthia from early to late period.

Stratigraphy and Paleoenvironment of Domi-1 and Sora-1 Wells, Domi Basin (도미분지 도미-1, 소라-1공의 층서와 고환경)

  • Yun, Hye-Su;Byun, Hyun-Suk;Oh, Jin-Yong;Park, Myong-Ho;Lee, Min-Woo
    • Economic and Environmental Geology
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    • v.45 no.4
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    • pp.407-429
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    • 2012
  • There has been much debates on the geologic age of the sediments of the Domi Basin, since age results varied after research methods and fossil groups. This study carried out palynological analysis and seismic interpretation to establish a stratigraphy and environmental reconstruction mainly based on fossil dinoflagellates and Seismic data from the Domi-1 and Sora-1 wells. The dinocyst assemblages found enabled zonation of the well sediment sequence resulting in 4 ecozones. Index fossils among dinocysts and palynomorphic substances indicate geologic age of the well ranges from Eocene to Pleistocene, and paleoenvironment varies from freshwater to inner-neritic marine. The fossil association also suggests strong relationship to Japanese Tertiary basins in Kyushu area in terms of stratigraphy and basin developmental history.

Effects of absorbents on growth performance, blood profiles and liver gene expression in broilers fed diets naturally contaminated with aflatoxin

  • Liu, J.B.;Yan, H.L.;Cao, S.C.;Hu, Y.D.;Zhang, H.F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.2
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    • pp.294-304
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    • 2020
  • Objective: The study was conducted to evaluate the effects of the absorbent (a mixture of activated carbon and hydrated sodium calcium aluminosilicate) on growth performance, blood profiles and hepatic genes expression in broilers fed diets naturally contaminated with aflatoxin. Methods: A total of 1,200 one-day-old male chicks were randomly assigned to 6 treatments with 10 replicate cages per treatment. The dietary treatments were as follows: i) control (basal diets); ii) 50% contaminated corn; iii) 100% contaminated corn; iv) control+1% adsorbent; v) 50% contaminated corn+1% absorbent; vi) 100% contaminated corn+1% absorbent. Results: During d 1 to 21, feeding contaminated diets reduced (p<0.05) body weight (BW), average daily gain (ADG), and average daily feed intake (ADFI), but increased (p<0.05) feed-to-gain ratio (F/G). The absorbent supplementation increased (p<0.05) BW, ADG, and ADFI. There were interactions (p<0.05) in BW, ADG, and ADFI between contaminated corn and absorbent. Overall, birds fed 100% contaminated diets had lower (p<0.05) final BW and ADG, but higher (p<0.05) F/G compared to those fed control diets. The absorbent addition increased (p<0.05) serum albumin concentration on d 14 and 28 and total protein (TP) level on d 28, decreased (p<0.05) alanine transaminase activity on d 14 and activities of aspartate aminotransferase and alkaline phosphatase on d 28. Feeding contaminated diets reduced (p<0.05) hepatic TP content on d 28 and 42. The contaminated diets upregulated (p<0.05) expression of interleukin-6, catalase (CAT), and superoxide dismutase (SOD), but downregulated (p<0.05) glutathione S-transferase (GST) expression in liver. The absorbent supplementation increased (p<0.05) interleukin-1β, CAT, SOD, cytochrome P450 1A1 and GST expression in liver. There were interactions (p<0.05) in the expression of hepatic CAT, SOD, and GST between contaminated corn and absorbent. Conclusion: The results suggest that the naturally aflatoxin-contaminated corn depressed growth performance, while the adsorbent could partially attenuate the adverse effects of aflatoxin on growth performance, blood profiles and hepatic genes expression in broilers.

Long-Term Monitoring of Noxious Bacteria for Construction of Assurance Management System of Water Resources in Natural Status of the Republic of Korea

  • Bahk, Young Yil;Kim, Hyun Sook;Rhee, Ok-Jae;You, Kyung-A;Bae, Kyung Seon;Lee, Woojoo;Kim, Tong-Soo;Lee, Sang-Seob
    • Journal of Microbiology and Biotechnology
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    • v.30 no.10
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    • pp.1516-1524
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    • 2020
  • Climate change is expected to affect not only availability and quality of water, the valuable resource of human life on Earth, but also ultimately public health issue. A six-year monitoring (total 20 times) of Escherichia coli O157, Salmonella enterica, Legionella pneumophila, Shigella sonnei, Campylobacter jejuni, and Vibrio cholerae was conducted at five raw water sampling sites including two lakes, Hyundo region (Geum River) and two locations near Water Intake Plants of Han River (Guui region) and Nakdong River (Moolgeum region). A total 100 samples of 40 L water were tested. Most of the targeted bacteria were found in 77% of the samples and at least one of the target bacteria was detected (65%). Among all the detected bacteria, E. coli O157 were the most prevalent with a detection frequency of 22%, while S. sonnei was the least prevalent with a detection frequency of 2%. Nearly all the bacteria (except for S. sonnei) were present in samples from Lake Soyang, Lake Juam, and the Moolgeum region in Nakdong River, while C. jejuni was detected in those from the Guui region in Han River. During the six-year sampling period, individual targeted noxious bacteria in water samples exhibited seasonal patterns in their occurrence that were different from the indicator bacteria levels in the water samples. The fact that they were detected in the five Korea's representative water environments make it necessary to establish the chemical and biological analysis for noxious bacteria and sophisticated management systems in response to climate change.

Studies on the Methods for the Isolation of salmonella from Pigs (돼지에서 살모넬라균(菌)을 분리(分離)하는 방법(方法)에 관한 연구(硏究))

  • Chung, G.T.
    • Korean Journal of Veterinary Research
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    • v.9 no.1
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    • pp.63-69
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    • 1969
  • Various methods for the isolation of salmonellae were compared by examining mesenteric lymph nodes and faeces of 395 pigs at slaughter. The conclusions were as follows. (i) Tetrathionate broth was significantly superior to selenite broth for the examination of mesenteric lymph nodes. (ii) There was no significant difference between tetrathionate broth and selenite broth for the examination of faeces. The use of both tetrathionate broth and selenite broth as enrichment media for faeces produced far better results than the use of any single enrichment medium. (iii) Brilliant green agar was significantly superior to SS agar for the examination of mesenteric lymph nodes. (iv) There was no significant difference between brilliant green agar and SS agar when faeces were examined. The use of both media produced far better results than the use of either singly. (v) Brilliant green MacConkey broth was much inferior to tetrathionate broth as an enrichment broth. Direct culture of faeces on brilliant green MacConkey agar yielded less isolates than prior enrichment in tetrathionate or selenite broth. (vi) The optimum incubation period of enrichment was 24 hours for faeces and 48 hours for mesenteric lymph nodes when either tetrathionate broth or selenite broth was used as enrichment media.

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Behcet`s Syndrome with Aortic Aneurysm: A Case Report (Bechet`s 병과 합병된 상부대동맥류: 치험 1례 보고)

  • Gang, Jeong-Ho;Lee, Jeong-Ho;Yu, Hoe-Seong
    • Journal of Chest Surgery
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    • v.10 no.1
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    • pp.98-105
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    • 1977
  • A 36 year old blindman, engineer was admitted with chief complaints of hemoptysis, recurrent sore throat, pyoderma in genital organ, uveitis and thrombophlebitis for 10 years. Above the chief complaints were remission or exacerbation during hospitalization. Physicalexamination showed that left radial, ulnar & brachial pulse was not palpable. No bruit or murmur was obtained over the mass. Neurologic examination revealed no significant finding.On admission, chest P-A showed hen egg sized round & oval compact hazy density on left upper lung field. Bronchogram revealed no pathological finding and Lt. tomogram showed well define large,ovoid mass density in the superior mediastinum. Fluoroscopy finding showed nonpulsatile on left upper lung field. Pre-op. aortography was not taken, under the impression of lung Ca. rule out .sortie aneurysm, exploratory operation was performed through the 2nd intercostal space, Lt. It was performed that the mass was ascending sortie aneurysm of saccular type. Direct aneurysmectomy with multiple figure of eight suture were done without any prosthetic graft. Post-op. control I.V.C graphy showed completely obstruction sign. Postopcontrol aortography revealed good surgical result. Final, histopathological answered non-specific sortie aneurysm, saccular type. Post-op. courses were uneventful except mild neurologic disturbance with subclavian steal syndrome and associated with both lower leg pitting edema due to inferior vena cava obstruction. After op, 3 month later, discharged to home, with big systemic problem. Behcet`s syndrome reviewed with related literatures. The coexistence of mouth and genital ulceration with hypopyon mentioned by hippocrates and described by various workers in the early part of this century was first defined as a syndrome by Behcet in 1937. In 1937 Behcet described a chronic relapsing triple symptom complex of oral ulceration, genital ulceration, and ocular inflammation. The place of the syndrome as part of a systemic disorder in now clearer, and the under lying pathology appears to be a vasculitis. The disease runs a- chronic course, blindness being the greatest disability and control nervous system involvement a cause of death. Thrombophlebitis is fairly frequent, france et al [1951] giving an incidence of 25% and Dowling [1961] 12%, superficial thrombophlebitis migrans and thrombosis of large veins, including venae cavae [Thomas, 1947: Boolukos 1960] are recorded. Little attention has been paid to arterial involvement. Mishima et al. [1961] described resection cf an aortic aneurysm in a 38 year old man with Behcet`s syndorme. Mounsey in a clinicopathological conference described a case [Brit, med. J., 1966] of ruptured aortic aneurysm in Bechcet`s syndrome treated by aorto-iliac graft. Also, Shikano and Oshima et al [1963] recorded two aneyrysm of smaller arteries. Unfrequently, aortic aneurysm was presumed to be secondary to osteomyelitis of the lumber spine, though the possible association between aortic aneurysm and Behcet`s syndrome was raised. A further case is reported here, in which ascending aortic aneurysm with Behcet`s Ds. appeared to form part of this generalized disease. This is a case report of surgical experience of Behcet`s Ds. with ascending aortic aneurysm which had nearly all the typical clinical features. Above mentioned and was reviewed with related literatures.

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A Study on the Horizontal Consolidation and Permeability Characteristics of Decomposed Mudstone Soil in Pohang (이암풍화토의 횡방향압밀 및 투수특성)

  • 김영수;김기영;백영식
    • Journal of the Korean Geotechnical Society
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    • v.16 no.1
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    • pp.31-42
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    • 2000
  • Consolidation and permeability are major engineering properties of soil. In clay, coefficient of permeability and consolidation can be calculated by incremental loading consolidation test. However, it is known that the incremental loading test has several deficiencies including long testing time, non-uniform stress state, very soft clay and problem of back pressure saturation. Specially, it is not performed with horizontal consolidation test. Several methods have been proposed for obtaining reliable values of $C_v$. Among these, the square root of time-fitting method proposed by Taylor(1948) and logarithm of time-fitting method, also called Casagrande's method, are used extensively in soil engineering practice. But these methods are not amenable for the absence of initial linear portion and have the difficulties involved in distinguishing secondary compression from primary compression. Rowecell consolidation tests were carried out in this study with different trimming axis and sample size. The results were compared with those of other methods; Casagrande,$Taylor,\; Casagrande,\; Hyperbolic,\; \delta/t-logt$. From the results, we explained a relationship between horizontal coefficient of permeability and void ratio was obtained. Finally, the directly measured horizontal coefficient of permeability obtained by using the Rowecell was compared with the permeability derived indirectly from the consolidation test result.

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