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Occurrence and treatment of mixed infections with Vbrio anguillarum and Ichthyobodo sp. from cultured oliver flounder (Paralichthys olivaceus) (양식 넙치에서 Vbrio anguillarum 및 Ichthyobodo sp.에 의한 혼합 감염증의 발생 및 치료)

  • Jang, Hwan;Moon, Jin-San;Joh, Seong-Joon;Kim, Ji-Yeon;Son, Seong-Wan
    • Korean Journal of Veterinary Research
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    • v.47 no.3
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    • pp.303-308
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    • 2007
  • The high mortality (65.5%) of young olive flounder (Paralichthys olivaceus) showing skin ulcers occurred in fish obtained from a commercial cultured farm at Donghae on the eastern coast of Korea at March 2006. The dorsal skin showed ulcer lesions and large amount of mucus. We isolated Ichthyobodo sp. from the gill, and Vibrio anguillarum from the liver and kidney of dead and diseased fish. All of the fish infected with the V anguillarum and Ichthyobodo sp. were treated with 100 ppm formalin for 1 h, and treated with oxolinic acid for 4 h at $22^{\circ}C$, respectively. The formalin treated group was observed the low mortality (2.9%) when compared with high mortality of non-treated (82.9%), and of antibiotic treated group (85.9%) against mixed infections by Vibrio anguillarum and Ichthyobodo sp. organisms. We confirmed complete elimination against mixed infections of Vibrio anguillarum and Ichthyobodo sp. with 100 ppm formalin treatment.

Antimicrobial Effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogen (발효 금은화 및 황련의 유해균 억제 효과)

  • Lee, Sin-Ji;Lee, Myeong-Jong;Jung, Ji-Eun;Kim, Ho-Jun;Bose, Shambhunath
    • Journal of Korean Medicine for Obesity Research
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    • v.11 no.1
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    • pp.35-46
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    • 2011
  • Objectives This study was designed to examine antimicrobial effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogens. Methods Lactobacilli MRS broth was added to 200mL glass bottle containing 20% herb powder(w/v) followed by 30 minute sonication and then shaking at 70 rpm in $70^{\circ}C$ water bath for 3 hours in order to extract fermented herb. Fermented herb extract was autoclaved at $121^{\circ}$ for 15minutes. $2{\times}10^7$ CFU/mL subcultured bacteria was inoculated and cultured for 24 hours and centrifuged at 3000 rpm for 5 minutes. After transferring to 15 mL conical tube, the viable cells were counted. Results and Conclusion Fermented Coptidis rhizoma and Lonicerae Flos both showed antimicrobial effect on pathogens especially when Fermented Coptidis rhizoma was experimented against Staphylococcus aureus.

Phosphoric Acid-doped SDF-F/poly(VI-co-MPS)/PTFE Membrane for a High Temperature Proton Exchange Membrane Fuel Cell

  • Lee, Jong-Won;Yi, Cheol-Woo;Kim, Keon
    • Bulletin of the Korean Chemical Society
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    • v.32 no.6
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    • pp.1902-1906
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    • 2011
  • Sulfonated poly(fluorinated arylene ether)s (SDF-F)/poly[(N-vinylimidazole)-co-(3-methacryloxypropyl-trimethoxysilane)] (poly(VI-co-MPS))/poly(tetrafluoroethylene) (PTFE) is prepared for a high temperature proton exchange membrane fuel cell (PEMFC). The reaction of the membrane with phosphoric acid forms silicate phosphor, as a chemically bound proton carrier, in the membrane. Thus-formed silicate phosphor, nitrogen in the imidazole ring, and physically bound phosphoric acid act as proton carriers in the membrane. The physico-chemical and electrochemical properties of the membrane are investigated by various analytical tools. The phosphoric acid uptake and proton conductivity of the SDF-F/poly(VI-co-MPS)/PTFE membrane are higher than those of SDF-F/PVI/PTFE. The power densities of cells with SDF-F/poly(VI-co-MPS)/PTFE membranes at 0.6 V are 286, 302, and 320 mW $cm^{-2}$ at 150, 170, and 190 $^{\circ}C$, respectively. Overall, the SDFF/poly(VI-co-MPS)/PTFE membrane is one of the candidates for anhydrous HT-PEMFCs with enhanced mechanical strength and improved cell performance.

Mechanical Properties of Electro-Discharge-Sintered Porous Titanium Implants (전기방전소결에 의해 제조된 다공성 Titanium 임플란트의 기계적 특성)

  • Hyun, C.Y.;Huh, J.K.;Lee, W.H.
    • Korean Journal of Materials Research
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    • v.16 no.3
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    • pp.173-177
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    • 2006
  • Porous surfaced Ti implant compacts were fabricated by electro-discharging-sintering (EDS) of atomized spherical Ti powders. Powders of $50-100{\mu}m$ in diameter were vibratarily settled into a quarts tube and subject to a high voltage and high density current pulse in Ar atmosphere. Single pulse of 0.7 to 2.0 kJ/0.7 gpowder, from 150, 300, and $450{\mu}F$ capacitors was applied in less than $400{\mu}sec$ to produce twelve different porous-surfaced Ti implant compacts. The solid core formed in the center of the compact shows similar microstructure of cp Ti which was annealed and quenched in water. Hardness value at the solid core was much higher than that at the particle interface and particles in the porous layer, which can be attributed to both heat treatment and work hardening effects induced by EDS. Compression tests were made to evaluate the mechanical properties of the EDS compacts. The compressive yield strength was in a range of 12 to 304MPa which significantly depends on input energy. Selected porous-surfaced Ti-6Al-4V dental implant compacts with a solid core have much higher compressive strengths compared to the human teeth and sintered Ti dental implants fabricated by conventional sintering process.

Characterization of Si/Mo Multilayer Anode for Microbattery (박막전지용 Si/Mo 다층박막 음극의 전기화학적 특성)

  • 이기령;정주영;문희수;이승원;이유기;박종완
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.11a
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    • pp.209-209
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    • 2003
  • The adventages of Li alloys have attracted the attention of many research groups, many of which have investigated tin-based alloys [1-2], Despite interesting performances of these, the irreversible capacity loss systematically observed on the first cycle for these compounds is a main drawback for their use as anode materials in lithium ion cells. Not only Sn is efficient in forming alloys with Li, Si can also react with Li to form alloys with a high Li/Si ratio, like Li$\_$22/Si$\_$5/ at 400$^{\circ}C$. It corresponds to a capacity of 4200mAh/g. Electrochemical Li-Si reaction occurs between 0 and 0.3 V against Li/Li$\^$+/, so that high-energy density battery can be realized. Despite the high theoretical capacity of elements like Si, however, particles of the alloys crack and fragment due to the repeated alloying and do-alloying which occurs as cell are charged and discharged. The research groups of Muggins [3] and Besenhard [4] have proposed that the volume expansion due to the insertion of Li can be reduced in micro- and submicro-structured matrix alloys. For this reason, the research group of J.R. Dahn investigated Sn/Mo sequential sputter deposition to prepare nanocomposites [5]. In this study, we investigated the characterization and the electrochemical characteristics of sequentially sputtered Si/Mo multilayer for microbattery anode.

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The Properties of Acetolactate Synthase Isozyme Produced by Serratia marcescens ATCC 254 19 (Serratia marcescens ATCC 25419가 생산하는 Acetolactate Synthase Isozyme의 특성)

  • 김종탁;김승수
    • Microbiology and Biotechnology Letters
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    • v.20 no.1
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    • pp.25-33
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    • 1992
  • One acetolactate synthase isozyme which has Rf value of 0.83 on polyacrylamide gel electrophoresis was purified from Sewatia marcescens ATCC 25419 by ammonium sulfate fractionation, DEAE-Sephacel chromatography, Phenyl-Sepharose chromatography, Sephacryt S-400 gel filtration followed by native gel elution. The native molecular weight of the enzyme was determined to be 531,400 by gel filtration method, and SDS-polyacrylamide gel electrophoresis separated the native enzyme into two polypeptides having molecular sizes of 55,000 and 38,900 respectively. In kinetic parameters, $K_m$ value for pyruvate was 2.54 mM, and $V_{max}$ was 21.75 nmoie/min/mg. The enzyme showed maximal activity around pH 8.0 and optimal temperature of the acetolactate formation was $37^{\circ}C$. Feedback inhibition studies indicate that the purified enzyme is rather resistant to branched chain amino acids when compared with acetolactate synthase isozymes of plants or other enterobacteria.

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A Study of the Relationship Analysis of Power Conversion and Changed Capacitance in the Depletion Region of Silicon Solar Cell

  • Kim, Do-Kyeong;Oh, Yeong-Jun;Kim, Sang-Hyun;Hong, Kyeong-Jin;Jung, Haeng-Yeon;Kim, Hoy-Jin;Jeon, Myeong-Seok
    • Transactions on Electrical and Electronic Materials
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    • v.14 no.4
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    • pp.177-181
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    • 2013
  • In this paper, silicon solar cells are analyzed regarding power conversion efficiency by changed capacitance in the depletion region. For the capacitance control in the depletion region of silicon solar cell was applied for 10, 20, 40, 80, 160 and 320 Hz frequency band character and alternating current(AC) voltage with square wave of 0.2~1.4 V. Academically, symmetry formation of positive and negative change of the p-n junction is similar to the physical effect of capacitance. According to the experiment result, because input of square wave with alternating current(AC) voltage could be observed to changed capacitance effect by indirectly method through non-linear power conversion (Voltage-Current) output. In addition, when input alternating current(AC) voltage in the silicon solar cell, changed capacitance of depletion region with the forward bias condition and reverse bias condition gave a direct effect to the charge mobility.

The Solution Structure of 18 residue YH motif Peptide within the Second fas-1 domain of ${\beta}ig-h3$

  • Han, Kyung-Doo;Son, Woo-Sung;Kim, Won-Je;Lee, Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.11 no.1
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    • pp.24-29
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    • 2007
  • [ ${\beta}ig-h3$ ] is an extracellular matrix protein that mediates cell adhesion through interaction with integrins. The 18 residue YH motifs within each fas-1 domain are known to be responsible for the interaction with the ${\alpha}_v{\beta}_5$ integrin, and the synthetic YH motif peptides are known to inhibit endothelial tube formation and reduces the number of blood vessels, and so expected to be an effective inhibitor of angiogenesis. In this study, we solved the 3D structure of the 18 residue YH motif peptide (EALRDLLNNHILKSAMCA; D2 peptide) within the second fas-1 domain of ${\beta}ig-h3$ using NMR. The Peptide has ${\alpha}-helix$ structure at the C terminal region but the N terminal region is flexible. The present structural information may be helpful for developing more effective peptide drug candidate for the treatment of diseases dependent on angiogenesis.

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A Design and Control of Bi-directional Non-isolated DC-DC Converter with Coupled Inductors for Rapid Electric Vehicle Charging System

  • Kang, Taewon;Kim, Changwoo;Suh, Yongsug;Park, Hyeoncheol;Kang, Byungil;Kim, Daegyun
    • Proceedings of the KIPE Conference
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    • 2011.07a
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    • pp.429-430
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    • 2011
  • This paper presents a simple and cost-effective stand-alone rapid battery charging system of 30kW for electric vehicles. The proposed system mainly consists of active front-end rectifier of neutral point clamped 3-level type and non-isolated bi-directional dc-dc converter of multi-phase interleaved half-bridge topology with coupled inductors. The charging system is designed to operate for both lithium-polymer and lithium-ion batteries. The complete charging sequence is made up of three sub-interval operating modes; pre-charging mode, constant-current mode, and constant-voltage mode. The pre-charging mode employs the staircase shaped current profile to accomplish shorter charging time while maintaining the reliable operation of the battery. The proposed system is able to reach the full-charge state within less than 16min for the battery capacity of 8kWh by supplying the charging current of 67A. The optimal discharging algorithm for Vehicle to the Grid (V2G) operation has been adopted to maintain the discharging current of 1C. Owing to the simple and compact power conversion scheme, the proposed solution has superior module-friendly mechanical structure which is absolutely required to realize flexible power expansion capability in a very high-current rapid charging system.

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The Analysis of Optical and Electrical Properties by On-Off Stress in Ceramic Metal-halide Lamp (세라믹 메탈할라이드 램프의 On-Off 스트레스에 의한 광학적, 전기적 특성 분석)

  • Kim, Woo-Young;Jang, Hyeok-Jin;Yang, Jong-Kyung;Lee, Se-Il;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1625_1626
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    • 2009
  • 최근 세라믹 메탈할라이드 램프의 사용이 증가함에 따라 고연색성, 장수명에 대한 기대치가 높아지고 있다. 이런 추세에 의하여 현재 정격수명이 15,000 ~ 20,000시간의 세라믹 메탈할라이드 램프가 개발되고 있으며 신뢰성에 대한 문제 또한 부각되고 있는 실정이다. 본 논문에서는 이러한 세라믹 메탈할라이드 램프의 수명 특성을 알아보기 위하여 "신뢰성기준 RS C 0085"를 바탕으로 램프를 20분 점등후 20분 소등을 한 주기로 하여 1,200회를 반복한 후 광학적 특성 및 전기적 특성을 측정하여 실험 전 초기 특성과 비교 분석하였다. 광학적 특성으로 광속과 효율은 3.1 [%] 감소, 연색성은 2.3 [%] 감소, 색온도 변화를 가져왔다. 전기적 특성으로는 램프 열화로 인한 내부저항의 변화로 인해 전압이 1.4 [V] 증가, 전류가 0.02 [A] 감소하였다.

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