• Journal of Plant Biotechnology
• /
• v.8 no.1
• /
• pp.43-50
• /
• 2006

The goal of this research was to develop an efficient cryopreservation protocol for germplasms of yam (Diosorea batatas), that were cultivated in Korea. Comparative studies with four other cryogenic techniques and subsequent experiments for shoot regrowth were conducted. in vitro-grown shoot-apices of the D. batatas were successfully cryopreserved by encapsulation-dehydration. The maximum survival of shoot-apices could be achieved when the precultured (with 0.3 M of sucrose for one day) and encapsulated (with a 3%(w/v) Na-alginate solution) apices were dehydrated for $3.5{\sim}4\;h$ prior to direct immersion in LN (liquid nitrogen). The frequency of regrowth rate of cryopreserved apices was not decreased during 3-month storage period. The thawing method markedly affected survival of the cryopreserved apices, and thawing at $40^{\circ}C$ for 3 min produced the best results. When cryopreserved apices were post-cultured on the post-culture medium (MS), supplemented with $0.2mgl^{-1}$ of BA ($N_6$-benzyladenine) and $0.2mgl^{-1}$ of kinetin, they showed direct shooting without callusing.

• 한국신재생에너지학회:학술대회논문집
• /
• 2011.05a
• /
• pp.90.1-90.1
• /
• 2011

연료전지 분리판은 연료, 공기, 수분이 흐를 수 있는 채널들이 포함되어 있으며, 전지들에 의해서 생산되는 전류를 흐르게 할 수 있는 전기전도성을 가져야 할 필요가 있다. 일반적인 금속판들은 연료전지 스택 내의 산성 분위기에 존재해야 하기 때문에 표면 부식이 쉽게 발생한다. 그라핀(graphene)은 우수한 전기전도성을 가지고 있을뿐만 아니라 물리화학적 내식성 및 내구성을 가지고 있어 연료전지 분리판으로서 응용이 가능할 것으로 판단된다. 본 연구에서는 일반적으로 널리 사용하고 있는 스테인리스강(stainless steel)을 모재로 사용하였으며, 그라핀을 전기분무법(electro spray coating)으로 코팅하여 스테인리스강의 내식성 및 전기전도성을 동시에 향상시키고자 하였다. 그라핀은 에탄올을 용매로 사용하여 분산하였으며, 분산제로 소량의 다이페닐다이에톡시실란(diphenyldiethoxysilane)을 첨가하여 코팅용액을 제작하였다. 코팅공정은 15kV 전압을 가하여 1시간동안 코팅을 진해하였으며, 그라핀-스테인리스강 모재의 미세구조를 전자현미경과 광학현미경을 통하여 관찰하였다. 또한 X-선 회절분석법을 이용하여 그라핀의 결정구조를 분석하였다. 한편 스택의 내부와 유사한 산화성 분위를 모사하기 위해 $80^{\circ}C$의 0.1N $H_2SO_4+2ppm\;F^-$ 용액에서 내식성 실험을 수행하였고, 면간접촉저항도 측정하였다. 그라핀이 코팅된 스테인리스강 시편은 고분자전해질 연료전지 분리판의 요구조건을 만족하였으며, 연료전지 분리판으로서의 적용가능성을 확인하였다.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.2
• /
• pp.236-244
• /
• 2014

The gene encoding ${\alpha}-\small{L}$-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that AbfA is an enzyme consisting of only a catalytic domain, and that the enzyme has significant similarity to AFases classified into the family 51 of the glycosyl hydrolases. abfA was subcloned into the pQE60 expression vector to fuse it with a six-histidine tag and the recombinant AFase (rAbfA) was purified to homogeneity. The specific activity of the recombinant enzyme was 96.7 U/mg protein. Determination of the apparent molecular mass by gel-filtration chromatography indicated that AbfA has a tetrameric structure. The optimal pH and temperature of the enzyme were 6.0 and $60^{\circ}C$, respectively. The enzyme activity was completely inhibited by 1 mM $HgCl_2$. rAbfA was active only towards p-nitrophephenyl ${\alpha}-\small{L}$-arabinofuranoside and exhibited $K_m$ and $V_{max}$ values of 3.5 mM and 306.1 U/mg, respectively. rAbfA showed a synergistic effect in combination with endoxylanase on the degradation of oat spelt xylan and wheat arabinoxylan.

• Structural Engineering and Mechanics
• /
• v.44 no.2
• /
• pp.239-255
• /
• 2012

This work presents a contribution to understand the inverted trussed beams behavior. The system has a main beam and struts with rectangular cross section associated to a wire rope enlaced to the main beam. It is an unpublished system with the advantage of easy positioning of the wire rope, once it is a continuous and connected by turnbuckles. It is a system that can be used as support for concrete formworks or for rehabilitation wooden beams proposal. The enlacement of the cable demands a small notch at the top of the cross section and a cross pin at the bottom. Six inverted trussed beams were tested, with spans of 180 cm with cables diameter of 1/4". Additionally, four simple beams without any external steel cable were also tested with material from the same lot of wood, allowing a comparison in rupture. The results showed capacity gain of around 60% compared to a simple beam. Once the wire rope characteristics and anchoring are very important for structure response, some improvement suggestions for the efficiency of the cables are also presented.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.6
• /
• pp.897-904
• /
• 2007

The aim of this paper is to discuss the methodology of our investigation of the dynamics of protein degradation and the total in situ protealytic activity in meso/eutrophic, eutrophic, and hypereutrophic freshwater environments. Analysis of the kinetics and rates of enzymatic release of amino acids in water samples preserved with sodium azide allows determination of the concentrations of labile proteins $(C_{LAB})$, and their half-life time $(T_{1/2})$. Moreover, it gives more realistic information on resultant activity in situ $(V_{T1/2})$ of ecto- and extracellular proteases that are responsible for the biological degradation of these compounds. Although the results provided by the proposed method are general y well correlated with those obtained by classical procedures, they better characterize the dynamics of protein degradation processes, especially in eutrophic or hypereutrophic lakes. In these environments, processes of protein decomposition occur mainly on the particles and depend primarily on a metabolic activity of seston-attached bacteria. The method was tested in three lakes. The different degree of eutrophication of these lakes was clearly demonstrated by the measured real proteolytic pattern and confirmed by conventional trophic state determinants.

• Journal of Powder Materials
• /
• v.22 no.5
• /
• pp.331-336
• /
• 2015

We report on the succesful fabrication of ZnO nanorod (NR)-based robust piezoelectric nanogenerators (PNGs) by using Cu foil substrate. The ZnO NRs are successfully grown on the Cu foil substrate by using all solution based method, a two step hydrothermal synthesis. The ZnO NRs are grown along c-axis well with an average diameter of 75~80 nm and length of $1{\sim}1.5{\mu}m$. The ZnO NRs showed abnormal photoluminescence specrta which is attributed from surface plasmon resonance assistant enhancement at specific wavelength. The PNGs on the SUS substrates show typical piezoelectric output performance which showing a frequency dependent voltage enhancement and polarity dependent charging and discharging characteristics. The output voltage range is 0.79~2.28 V with variation of input strain frequency of 1.8~3.9 Hz. The PNG on Cu foil shows reliable output performance even at the operation over 200 times without showing degradation of output voltage. The current output from the PNG is $0.7{\mu}A/cm^2$ which is a typical out-put range from the ZnO NR-based PNGs. These performance enhancement is attributed from the high flexibility, high electrical conductivity and excellent heat dissipation properties of the Cu foil as a substrate.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 1993.10a
• /
• pp.1043-1052
• /
• 1993

Field experiments were conducted to determine the optimum combination of performance parameters of a single-shank, tractor-mounted oscillating subsoiler. Tests were conducted at frequencies of oscillation of 3.7 , 5.67, 7.58, 9.48 and 11.456Hz ; amplitudes of 18, 21, 23.5, 34 and 36.5 mm ; and forward speeds of 1.84, 2.19 and 3.42 kmph at moisture content close to the plastic limit of the soil. It was observed that there was a reduction in average draft but an a increase in average total power requirement for oscillating than non-oscillating subsoiling. The draft and power ratios were significantly affected by the forward speed, frequency and amplitude. Their combined interaction expressed in terms of the velocity ratio parameter( the ratio of peak tool velocity and forward speed) however has the strongest influence. At the same velocity ratio, the draft reduction and power increase were less at higher amplitude of oscillation . As the oscillating frequency is increased toward the soil resonance the draft requirement becomes less. For the field conditions tested. the optimum operation was obtained at an amplitude of 36.5mm, frequency of 9.48Hz and speed of 2.19 kmph with a draft ratio of 0.33 and a power ratio of only 1.24.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.3
• /
• pp.354-357
• /
• 1999

Hypothyroidism was induced in three age groups (8 months, 1 year and 2 years) of Black Bengal goats by administering thiourea subcutaneously for 30 days. From fourth day onwards the animals were observed to be in hypothyroid state. For 8 months age group, a control group of similar age was taken. For the 1 year and 2 years age groups there was no control group but the values obtained on the pre-treatment day were considered as control values. For confirming the establishment of hypothyroidism, peripheral plasma triiodothyronine $(T_3)$ and thyroxine $(T_4)$ were estimated in all the animals before and during the treatment period by radioimunoassay. In the thiourea treated 8 months age group the $T_3$ and $T_4$ levels were significantly (p<0.001) lower than those in the control group during the treatment period. In all the thiourea treated groups the $T_3$ and $T_4$ levels observed during the treatment period were significantly (p<0.01) lower than the pre-treatment levels. A nonsignificant loss in the body weight was observed in all the thiourea treated groups whereas there was a significant growth in the control group of animals. The study indicates that hypothyroidism has a negative effect on growth in goats.

• 한국연소학회:학술대회논문집
• /
• 2007.05a
• /
• pp.19-24
• /
• 2007

The deposition behavior of soot particles in a diffusion flame along a solid wall was examined experimentally by getting rid of the effect of natural convection utilizing microgravity environment. The microgravity environment was realized by using a drop tower facility. The fuel for the flame was an ethylene ($C_2H_4$) and the surrounding oxygen concentration 35% with the surrounding air velocity of $V_a$=2.5, 5, and 10 cm/s. Laser extinction method was adopted to measure the soot volume fraction distribution between the flame and burner wall. The results show that observation of soot deposition in normal flame was difficult from buoyancy and the relative position of flame and solid surface changes with time. The soot particle distribution region moves closer to the surface of the wall as the surrounding air velocity is increased. And the experiments determined the trace of the maximum soot concentration line. It was found that the distance between soot line and flame line is around 5 mm. That is, the soot particle near the flame zone tends to move away from flame zone because of thermophoretic force and to concentrate at a certain narrow area inside of the flame, finally, to adhere the solid wall.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.10 no.1
• /
• pp.51-59
• /
• 2005

Genetic diversity within and between populations of Antheraea mylitta Drury was studied using thirty individuals from three ecoraces using 12 ISSR and 10 RAPD primers. Rally, Daba and Modal ecoraces were collected from Chattisgarh, Jharkhand and Orissa states of India respectively. The ISSR and RAPD primers generated $94.7\%$ and $95.6\%$ polymorphism among the 30 individuals. The cluster analysis grouped these individuals according to their ecorace. The intra-ecoracial heterozygosity estimated with ISSR markers were $0.123{\pm}0.18,\;0.169{\pm}0.17\;and\;0.214{\pm}0.17$ respectively for Modal, Raily and Daba ecoraces. Like wise, with RAPD markers the intraecoracial heterozygosity was $0.17{\pm}0.22$ in Modal, $0.229{\pm}0.17$ in Raily and $0.23{\pm}0.19$ in Daba ecoraces. However, the significantly low genetic differentiation (GST) (0.182 for ISSR and 0.161 for RAPD) and the high gene flow (Nm) (2.249 for ISSR and 2.60 for RAPD markers) among the ecoraces revealed that the amount of genetic diversity present among the ecoraces is not significant enough to make drastic genetic drifts among these ecoraces in the near future.