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Lactic Acid Fermentation of Gamju Manufactured Using Medicinal Herb Decoction (약초 추출액을 사용하여 제조한 감주의 젖산발효)

  • Cho, Kye-Man;Ahn, Byung-Yong;Seo, Weon-Taek
    • Korean Journal of Food Science and Technology
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    • v.40 no.6
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    • pp.649-655
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    • 2008
  • In this study, the characteristics of the lactic fermentation of gamju manufactured using a medicinal herb decoction were assessed. A bacterial strain, LAB19, which is used for the induction of lactic fermentation into gamju, was isolated from dried persimmon and identified as Leuconostoc mesenteroides on the basis of morphological, physiological, and chemotaxonomical features, and 16S rRNA sequencing analysis. After 60 hours of lactic fermentation with Leuconostoc mesenteroides LAB19 at $25^{\circ}C$, the gamju was determined to contain 141.3 g/L of reducing sugar, 5.33 g/L of acids, and 1.19 g/L of soluble phenolics. Approximately 90% of reducing sugar and 58% of acids were maltose and lactic acid, respectively. Free radical scavenging activities were retained at levels between 76.6 to 75.7% during the lactic fermentation of gamju.

입자 가속기 MC50과 C30 사이클로트론의 운영 현황 및 RI 빔 인출 연구

  • Yu, In-Gong;Lee, Ji-Seop;Park, Hyeon;Han, Jun-Yong;Jo, Seong-Jin;Lee, Min-Yong;Hwang, Won-Taek;Yang, Tae-Geon;Kim, Jae-Hong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.173-173
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    • 2012
  • 사이클로트론은 암진단에 사용되는 방사성동위원소를 생산하기 위한 중요한 입자 가속장치이다. 현재 핵의학 의료진단에 필요한 방사성동위원소를 제공하기 위해 세계적으로 사이클로트론의 활용도가 점점 증가하고 있다. 한국원자력의학원에 설치된 MC50 (양성자 최대 가속에너지 50 MeV, 60 uA)과 C30 (양성자 최대 에너지 30 MeV, 250 uA) 사이클로트론은 생명의학, 반도체 검출기, 핵자료 데이터, 방사성동위원소 개발 등 다양한 분야의 연구를 지원하고 있다. MC50 사이클로트론은 수소 입자를 포함하여 중양자, 알파 입자를 가속할 수 있으며 중성자 빔을 인출 할 수 있다. 수소 음이온 또는 양이온을 가속 할 수 있으며 표적에는 고에너지의 양이온이 조사되며, 핵반응을 통해 방사성동위원소가 생성된다. 양성자 빔을 이용하여 암세포를 사멸 시키는 치료법, 돌연변이로 새로운 종의 개발 등 다양한 응용성이 있다. 하전입자를 가속하는 사이클로트론의 주요 구성요소는 (1) 진공시스템, (2) 전자석 시스템, (3) 고주파 시스템, (4) 이온원 (5) 빔 인출장치 (6) 빔전환 장치 (수직에서 수평 방향으로 전환), (7) 빔 집속 및 진단 장치 등 이다. 본 발표에서는 85년부터 운영한 MC50 사이클로트론과 02년부터 가동된 사이클로트론의 운영 현황 및 다양한 응용분야와 향후 RI 빔 인출을 위한 계획을 소개하고자 한다.

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Mineralogy of Clinopyroxene from the Geodo Mine (거도광산의 단사휘석에 관한 광물학적 연구)

  • 최진범;김수진
    • Journal of the Mineralogical Society of Korea
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    • v.2 no.1
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    • pp.26-36
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    • 1989
  • Clinopyroxene in the Geodo mine belongs to diopside-hedenbergite series. It is widely distributed throughout the mine area together with garnet and is also closely related with Fe-mineralization. Clinopyroxenes in the Geodo mine including two samples from the sangdong and Ulchin Mines are studied using polarized microscope, EPMA, XRD, and IR spectroscopy for occurrence, chemistry, structure, and crystal chemistry. Especially, variations in unit-cell parameters are examined in relation with the substitution scheme between Fe and Mg cations. Clinopyroxenes in the Geodo mine occur in both endoskarn and exoskarn zone. It is mostly anhedral to subhedral with fine- to medium-grained in texture, but some have bigger crystals of short prismatic or columnar habits. Clinopyroxene occurs as monomineralic or is associated with mostly garnet and sometimes with actinolite, magnetite, epidote, and chlorite. Chemical analysis reveals that the Geodo clinopyroxene is diopsidic in composition (Di: 65-96%). This fact is in good contrast with garnet chemistry showing mostly andraditic (An: 41-82%). Especially, clinopyroxene coexisting with magnetite belongs to nearly end member diopside (Di: 97-99%). Thus, diopside-andradite pair indicates that Geodo skarns were formed under the reduced environment. X-ray diffraction analysis shows unit-cell parameters vary with increase of Fe contents: a = 9.765-9.838$\AA$, b = 8.943-9.020$\AA$, c= 5.240-5.253$\AA$.$\beta$ = 105.70-104.83$^{\circ}$, and V =440.64-448.19$\AA$3. It is noted from the least square regression that a, b and V increase linearly with increase of Fe content, while $\beta$ slightly decreases and c remains nearly unchanged as change in Fe content. These trends are to difference between synthetic and natural clinopyroxenes. This fact is also recognized in IR spectra which show a slight shift of several absorption bands toward lower wavenumber region with increasing Fe content.

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Comparison of Anthocyanin Content in Seed Coats of Black Soybean [Glycine max(L.) Merr.] Cultivars Using Liquid Chromatography Coupled to Tandem Mass Spectrometry

  • Shin, Sung-Chul;Lee, Soo-Jung;Lee, Sung-Joong;Chung, Jong-Il;Bae, Dong-Won;Kim, Soo-Taek;Sung, Nak-Ju
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1470-1475
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    • 2009
  • The seed coat of the black soybean contains 3 main anthocyanins such as delphinidin-3-O-$\beta$-glucoside, cyanidin-3-O-$\beta$-glucoside, and petunidin-3-O-$\beta$-glucoside. As a part of our effort on discovering and breeding new black soybean cultivars which possesses specific anthocyanin component rich, we determined the anthocyanin profiles of the 2 cultivars recently developed soybean cv. Gaechuck #1 and cv. Gyeongsang #1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared their content and identity with those of previously known 10 cultivar controls. The Cosmosil-$5C_{18}$-AR-II column were selected for the analysis because of the best peak separation. The column temperature was set up at $35^{\circ}C$. The mobile phase consisting of water containing 0.5%(v/v) formic acid and methanol gave good separation between the 3 anthocyanin analytes and internal standard (quercetin 3-O-$\beta$-rutinoside) and peaks with suppressed tail. The MS/MS spectra of each individual anthocyanin standard were detected in positive electron spray ionization (ESI) modes. It was disclosed that the anthocyanin contents of the soybean cv. Gaechuck#1 and cv. Gyeongsang#1 are roughly higher than those of the 10 controls.

Electrochemical Characteristics and Physical Properties of Poly(ethylene oxide)-Li based Polymer Electrolyte (Poly(ethylene oxide)-Li계 고분자 전해질의 전기화학적 특성 및 물리적 성질)

  • Kim, Hyung-Sun;Cho, Byung-Won;Yun, Kyung-Suk;Chun, Hai-Soo
    • Applied Chemistry for Engineering
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    • v.7 no.3
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    • pp.433-442
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    • 1996
  • Electrochemical characteristics and physical properties of polymer electrolyte which immobilized lithium salts such as $LiClO_4$ and $LiCF_3SO_3$ and plasticizers such as ethylene carbonate(EC) and propylene carbonate(PC) in high molecular weight poly(ethylene oxide)[PEO] polymer was investigated. PEO-Li based polymer electrolyte with plasticizers showed ionic conductivity of $10^{-4}S/cm$ at room temperature and high electrochemical stability up to 4.5 V(vs. $Li^+/Li$), so it can be applied to lithium secondary battery. The crystallinity of PEO decreased with the addition of lithium salts and plasticizers, especially $LiClO_4$ and PC showed more effective than and $LiCF_3SO_3$ and EC. Glass transition temperature($T_g$) of polymer electrolyte increased with increasing lithium salt concentration whereas melting temperature ($T_m$) decreased. Polymer electrolyte with plasticizers crystallized at $6^{\circ}C$.

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Oospore Production in Broth Media and Oospore Germination of Phytophthora capsici (고추 역병균(Phytophthora capsici)의 액체배지에서 난포자 형성과 발아)

  • Kim, Byung-Sup;Rin, Ernest James;Coffey, Michael D.
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.114-116
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    • 2009
  • In this study, we selected suitable broth media for mass production of Phytophthora capsici oospore, investigated oospore germination and secured $F_1$ progeny. Carrot broth and V8C broth were determined most effective for oospore formation by calculating and comparing oospore concentration produced from 8 different liquid media. Eleven strains were selected from P. capsici (CapA)/P. tropicalis (CapB) and 9 crosses were formed. Oospore progeny were produced, isolated and germinated from A1 and A2 combinations of P. capsici (CapA) with P tropicalis (CapB). This resulted in a total number of 129 $F_1$ isolates of P. capsici/P. tropicalis with a 0.64-4.0% (mean 1.85%) oospore germination.

Pressure Effect on the Dissociation Reactions of Some Weak Acids (약산의 해리반응에 미치는 압력의 영향)

  • J. U. Hwang;J. J. Chung;Y. T. Park;J. G. Jee;E. S. Park
    • Journal of the Korean Chemical Society
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    • v.27 no.5
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    • pp.311-319
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    • 1983
  • The dissociation constants of anilinium ion, o-toluidinium ion, phenol and o-chlorophenol were measured spectroscopically in aqueous solution over the temperature range of 10∼40$^{\circ}C$ and at the pressures up to 2,000 bars. The effects of temperature and pressure on the dissociation of the phenols are the same as those of the ordinary weak acids. Meanwhile the dissociation constants of anilinium ions are increased with temperature raising and decreased with pressure elevation. The effects of pressure on the constants can be explained by taking account of change of charge during dissociation reaction; there are increase in charge in the dissociation of phenols but anilinium ions are not. Several thermodynamic properties, ${\Delta}H^{circ}$,${\Delta}G^{circ}$, ${\Delta}S^{circ}$,${\Delta}V^{circ}$ and ${\beta}$ are calculated from those constants, and the dissociation reactions are discussed by them.

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Effects of PCB ENIG and OSP Surface Finishes on the Electromigration Reliability and Shear Strength of Sn-3.5Ag PB-Free Solder Bump (PCB의 ENIG와 OSP 표면처리에 따른 Sn-3.5Ag 무연솔더 접합부의 Electromigration 특성 및 전단강도 평가)

  • Kim, Sung-Hyuk;Lee, Byeong-Rok;Kim, Jae-Myeong;Yoo, Sehoon;Park, Young-Bae
    • Korean Journal of Materials Research
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    • v.24 no.3
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    • pp.166-173
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    • 2014
  • The effects of printed circuit board electroless nickel immersion gold (ENIG) and organic solderability preservative (OSP) surface finishes on the electromigration reliability and shear strength of Sn-3.5Ag Pb-free solder bump were systematically investigated. In-situ annealing tests were performed in a scanning electron microscope chamber at 130, 150, and $170^{\circ}C$ in order to investigate the growth kinetics of intermetallic compound (IMC). Electromigration lifetime and failure modes were investigated at $150^{\circ}C$ and $1.5{\times}10^5A/cm^2$, while ball shear tests and failure mode analysis were conducted under the high-speed conditions from 10 mm/s to 3000 mm/s. The activation energy of ENIG and OSP surface finishes during annealing were evaluated as 0.84 eV and 0.94 eV, respectively. The solder bumps with ENIG surface finish showed longer electromigration lifetime than OSP surface finish. Shear strengths between ENIG and OSP were similar, and the shear energies decreased with increasing shear speed. Failure analysis showed that electrical and mechanical reliabilities were very closely related to the interfacial IMC stabilities.

Characteristics of mycelial growth and fruit body of Sparassis latifolia strains and selection of suitable incubation conditions in liquid spawn

  • Lee, Yunhae;Gwon, Heemin;Jeon, Daehoon;Choi, Jongin;Lee, Youngsoon
    • 한국균학회소식:학술대회논문집
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    • 2018.05a
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    • pp.29-29
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    • 2018
  • Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

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Kinetic Studies on Hydration of Traditional and High-Yielding Rice Varieties (일반쌀 및 다수확 쌀의 수화속도)

  • Lee, Soon-Ock;Kim, Sung-Kon;Lee, Sang-Kyu
    • Applied Biological Chemistry
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    • v.26 no.1
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    • pp.1-7
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    • 1983
  • The hydration of two japonica(Akibare and Milyang 15) and four indica(Milyang 30, Suweon 287, Suweon 294 and Iri 342) rice varieties was investigated in terms of mathematical rate equation. The hydration rate at temperatures of $4{\sim}32^{\circ}C$ was examined by a weighing method. The absorption of water was directly proportiponal to the square root of the hydration time(t) and was described by the diffusion equation: $1-\bar{M}=(2/\sqrt{\pi})(S/V)\;\sqrt{Dt},\;where\;\bar{M}$ is dimensionless moisture ratio, S/V is the surface-to-volume ratio and D is diffusion coefficient. The average D value was given by the Arrhenius relation: $D=D_0\;\exp(-E_a/TR)$. The activation energy was $4{\sim}5kcal/mole$. The rice samples could be classified into three groups based on hydration kinetics: Milyang 30-Suweon 287; Akibare-Milyang 15; and Suweon 294-Iri 342.

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