• Journal of Animal Science and Technology
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• v.64 no.3
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• pp.481-499
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• 2022

This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose-galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.

• The Korean Journal of Physiology
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• v.27 no.1
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• pp.37-49
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• 1993

Ventilatory responses to inhaled $CO_2$ were measured during continuous negative pressure breathing (CNPB) in awake dogs. End expiratory lung volume (EELV) decreased linearly with pressure level during CNPB (correlation coefficient= 0.81, p<0.005) during air breathing. When CNPB was applied during 5% $CO_2$ inhalation, the decrease in EELV was not significantly different (p<0.5) from that during air breathing. As a result of a lowered EELV, tidal volume ($V_T$) significantly decreased by 22% and breathing frequency ($f_B$) increased by 68% in the steady state during air breathing (p<0.0001). These responses were similar during 5% $CO_2$ inhalation, thus the $CO_2$ response curve measured during CNPB shifted upward without a change in sensitivity (p>0.05). These results indicate additive effects of CNPB and $CO_2$ inhalation. The degree of hyperventilation during CNPB at eupnea was estimated to be 63% of that during control ventilation and was significantly greater than zero (p<0.0001), which suggests an alveolar hyperventilation due to CNPB. These results suggest that the mechanical alterations associated with n decrease in lung volume could play an important role in ventilatory control independently of chemical regulation of breathing. Thus, exercise hyperpnea, which is associated with a lowered functional residual capacity (FRC), may in part be explained by this mechanical stimulation of breathing.

• International Journal of Automotive Technology
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• v.1 no.1
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• pp.17-25
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• 2000

The electromechanical valve train (EMV) technology allows for a reduction in fuel consumption while operating under a stoichiometric air-fuel-ratio and preserves the ability to use conventional exhaust gas aftertreatment technology with a 3-way-catalyst. Compared with an engine with a camshaft-driven valve train, the variable valve timing concept makes possible an additional optimization of cold start, warm-up and transient operation. In contrast with the conventionally throttled engine, optimized control of load and in-cylinder gas movement can be used for each individual cylinder and engine cycle. A load control strategy using a "Late Intake Valve Open" (LIO) provides a reduction in start-up HC emissions of approximately 60%. Due to reduced wall-wetting, the LIO control strategy improves the transition from start to idle. "Late Exhaust Valve Open" (LEO) timing during the exhaust stroke leads to exhaust gas afterburning and, thereby, results in high exhaust gas temperatures and low HC emissions. Vehicle investigations have demonstrated an improved accuracy of the air-fuel-ratio during transient operation. Results in the New European Driving Cycle have confirmed a reduction in fuel consumption of more than 15％ while meeting EURO IV emission limits.

• Proceedings of the KIPE Conference
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• 2011.07a
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• pp.97-98
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• 2011

The HVAC(Heating Ventilation and Air conditioning) system is controlled by two ways, one is ON/OFF control and the other is PWM inverter with V/F. Control of blower with the use of PWM inverter has quite some benefits such as the capability of changing speed, high efficiency and reduced noise level compared with ON/OFF control. But if blower is operated at low speed, high THD generated by decrease of ma, and output voltage lowered in proportion to frequency. To solve these problems, filter should be installed at the output stage of inverter, which can decrease THD but has problems such as increase of volume size and additional braking resistance. This paper proposes the PWAM method which can reduce THD instead of installing the filter at the output stage of inverter. The proposed PWAM method is an inverter modulation method that fixes the modulation index of inverter to reduce THD by varying DC link voltage of inverter unlike conventional PWM method. Finally, the validity of proposed PWAM methods verified by experiments.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.8
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• pp.1115-1119
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• 2013

The carbon nanotube/poly-vinylidene fluoride (CNT/PVDF) composite films for the use of electronic devices were fabricated by spray coating method using the CNT/PVDF solution, which was prepared by adding PVDF pellets into the CNT dispersed N-Methyl-2-pyrroli-done (NMP) solution. The CNT/PVDF composite films were peeled off from the glass substrate and were investigated by the scanning electron microscopy, which revealed that the CNTs were uniformly dispersed in the PVDF films and thickness of the films were approximately $20{\mu}m$. The capacitance of the CNT/PVDF films increased dramatically by adding CNTs into the PVDF matrix, and finally saturated approximately 1880 pF. However, the I-V curves didn't show any saturation effect in the CNT concentration range of 0 ~ 0.04 wt%. Therefore we can control the performance of the devices from the CNT/PVDF composite film by adjusting the current level resulted from the CNT concentration with the uniform capacitance value.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.40 no.2
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• pp.162-172
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• 1991

In proportion to the capacity enlargement of the induction motor system controlled by current source inverter, the capacitance of the commutating capacitor is enlarged and then the spike value of output voltage is increased at the moment of charge and discharge. Moreover, the output currnet includes a number of harmonic components. Such voltage spike and harmonics generate the torque ripple and lead to bad effects on the performance of the induction motor. In this study, all the harmonics excluding 17th and 19th harmonics were mostly elimunated by adopting 18-phase Triple High Frequency Current Source Inverter(HFCSI), and the spike component of output voltage was reduced by adding the Voltage Clamping Circuit(VCC). As a result, the torque ripple and the commutation loss were reduced and the performance of the system was improved. Experiments for speed control were carried out in the tripple current source inverter system for induction motor drive. Overall system was controlled by ONE CHIP MICROCOMPUTER(INTEL 8751). Control circuits were simplified and good experimental results in the constant V/F control were obtained due to the flexibility of the microcomputer.

• Journal of Advanced Navigation Technology
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• v.16 no.5
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• pp.731-737
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• 2012

In the present study, a feasibility study on an innovative satellite attitude control actuator is performed. The actuator is specially designed to generate the reaction torque in an arbitrary axis, so that a satellite attitude can be controlled by using itself. It consists of a spherical flywheel and electromagnets for levitation and rotation control of the ball. As the earlier study, a rotating performance test on the spherical actuator is conducted in a single rotating axis and vertical levitation condition. From the test results, it can be confirmed that the maximum speed and torque of the innovative device are 7,200rpm and 0.7Nm, respectively. Using a velocity-voltage characteristic curve of the spherical motor, an open-loop control (V/f constant control) is performed, and the test results show excellent control performance in acceleration and deceleration phases.

• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.1
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• pp.57-61
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• 2007

For developing an integrated eco-friendly package against root knot disease complex of mulberry caused by the association of Meloidogyne incognita with Fusarium solani and F. oxysporum causing serious loss in terms of leaf yield and quality during cultivation, twenty botanical extracts at 5, 10 & 20% concentrations were screened under in vitro conditions. Among the extracts, Allium sativum followed by Lasownia inermis were found to be effective at 20% concentration against both the virulent fungi and nematode. Both the extracts reduced the mycelial growth of virulent fungi to an extent of 76-100%, inhibited the hatching of nematode eggs by 80-90% and 76-85% larval mortality over the control. The other extracts were found either moderately or poorly effective in reducing the growth of fungi, hatching of nematode eggs and enhancing the mortality of larvae. The two effective botanical extracts, which rated as strong inhibitors against both nematode and virulent fungi, can be utilized in developing an integrated ecofriendly technology for better management of root knot disease complex in mulberry.

• Journal of Korean Society of Water and Wastewater
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• v.32 no.1
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• pp.19-25
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• 2018

The objectives of this study were to evaluate the removal characteristics of total nitrogen, the influence factor of denitrification and the optimum operating condition in the pigment wastewater treatment using PAC-A/O process. The operating conditions of PAC-A/O process were mean BOD volumetric loading $0.86kgBOD/m^3/day$, mean F/M ratio 0.072~0.13 kgBOD/kgMLVSS/day and mean C/N ratio 3.47, respectively. The conditions of anoxic process in the field plant test were mean pH 8.3~8.7 and mean temperature $34.1{\sim}44.0^{\circ}C$. The ORP bending point knee was eventually appeared in the ORP -107 mV and $NO_3{^-}-N$ removal efficiency was increased according to the ORP decrease. In the ORP -107 mV below condition, the removal efficiency of T-N and $NO_3{^-}-N$ was 92.3~95.0% and 98.5~99.7%. Denitrification rate was calculated to be 1.581~1.791 mg $NO_3{^-}-N/gMLSS/hr$. The experimental results showed that the ORP control in the PAC-A/O process could be an effective method for treatment of pigment wastewater.

• Proceedings of the KIEE Conference
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• 2006.07b
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• pp.974-975
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• 2006

본 논문에서는 대용량 전력 변환 장치이며 출력 전압 가변이 가능한 H-브릿지 멀티레벨 인버터의 제어 알고리즘을 제안한다. H-브릿지 멀티레벨 인버터는 독립적으로 절연된 직류 부를 갖는 저압의 단상 인버터(셀 유닛)를 다수 직렬 접속하여 고압 3상 전압을 출력하는 Cascaded H-bridge 전압형 인버터이다. 다수의 독립 절연 직류부를 만들기 위해 입력 측에 다권선 변압기를 사용하여 입력 측 고조파 함유율을 축소시킬 수 있다. 인버터를 사용하는 제어방법 중 가장 오래되고 일반적인 방법으로 출력전압과 출력 주파수의 비를 일정하게 제어하여 전동기의 자속 크기를 일정하게 제어하고, 별도의 센서 없이도 운전이 가능한 V/F 제어모드는 저속 모드에서 제어 성능이 떨어지는 것을 실험을 통해 확인할 수 있었다. 정격용량 180kVA이고 출력 전압 480V인 H-브릿지 멀티 레벨 인버터 실험을 통해 벡터제어와 센서리스 벡터제어를 구현하여 저속 모드에서 제어 성능의 향상을 입증하였다.