• The Korean Journal of Physiology and Pharmacology
• /
• v.21 no.2
• /
• pp.251-257
• /
• 2017

Inhibition of $K^+$ outward currents by linopirdine in the outer hair cells (OHCs) of circling mice (homozygous (cir/cir) mice), an animal model for human deafness (DFNB6 type), was investigated using a whole cell patch clamp technique. Littermate heterozygous (+/cir) and ICR mice of the same age (postnatal day (P) 0 -P6) were used as controls. Voltage steps from -100 mV to 40 mV elicited small inward currents (-100 mV~-70 mV) and slow rising $K^+$ outward currents (-60 mV~40 mV) which activated near -50 mV in all OHCs tested. Linopirdine, a known blocker of $K^+$ currents activated at negative potentials ($I_{K,n}$), did cause inhibition at varying degree (severe, moderate, mild) in $K^+$ outward currents of heterozygous (+/cir) or homozygous (cir/cir) mice OHCs in the concentration range between 1 and $100{\mu}m$, while it was apparent only in one ICR mice OHC out of nine OHCs at $100{\mu}m$. Although the half inhibition concentrations in heterozygous (+/cir) or homozygous (cir/cir) mice OHCs were close to those reported in $I_{K,n}$, biophysical and pharmacological properties of $K^+$ outward currents, such as the activation close to -50 mV, small inward currents evoked by hyperpolarizing steps and TEA sensitivity, were not in line with $I_{K,n}$ reported in other tissues. Our results show that the delayed rectifier type $K^+$ outward currents, which are not similar to $I_{K,n}$ with respect to biophysical and pharmacological properties, are inhibited by linopirdine in the developing (P0~P6) homozygous (cir/cir) or heterozygous (+/cir) mice OHCs.

• Journal of Radiation Protection and Research
• /
• v.10 no.1
• /
• pp.14-28
• /
• 1985

The development of mono energetic photon sources using $K_{\alpha}$ fluorescence X-ray of pure material was carried out in the energy range below 100 keV. The monoenergetic photons are very useful in the calibration of the radiation measuring instruments and can be produced as the $K_{\alpha}$ fluorescence X-ray by irradiating the bremsstrahlung to the thin pure metal foils called ‘radiators’. In this experiment, several radiators such as $_{47}Ag,\;_{50}Sn,\;_{68}Er,\;_{70}Yb,\;and\;_{82}Pb$ provide the wide monoenergetic photon energy ranging from 20 keV to 80 keV. By the spectrometry with HpGe LEPS, spectral purity factors which measure the monochrometicity for the $K_{\alpha}$ fluorescence X-ray, were determined as $0.64{\sim}0.94$. Dosimetry for the purpose of the determination of the exposure rate with a 600cc thin window ionization chamber, which was calibrated by the standard free-air ionization chamber, was performed. Exposure rates ranging $8.3{\sim}232.5mR/h$ was obtained according to the $K_{\alpha}$ fluorescence X-ray energy for each radiator.

• Journal of fish pathology
• /
• v.20 no.3
• /
• pp.237-247
• /
• 2007

In 1999, Vibrio harveyi infection occurred among cultured marine finfishes including olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), and turbot (Scophtalmus maximus) in the province of Gyeongsang, Korea. We examined the various microbiological characteristics of this V. harveyi strains. V. harveyi was grown well in the 3% NaCl at 30℃. The swarming activity appeared to be an one of the characteristic properties of the V. harveyi was the highest in the 2% NaCl concentration at TSA medium. These cells were elongated and had the several lateral flagella which is not waved and shorter than polar flagella. It was not luminescent in the all isolated strains. Whole cell of the V. harveyi had a major protein of 50 kDa and presented various band around of 40 kDa in strains.

• Food Science and Preservation
• /
• v.17 no.1
• /
• pp.84-90
• /
• 2010

We investigated methods to minimize patulin content during processing of apple juice in Korea. Ascorbic acid concentration (100, 200, 300, and 400 ppm) and activated carbon concentration (50, 100, 150, and 200 ppm) were the parameters assessed by response surface methodology. Residual patulin was lowest in the presence of 237.77 ppm ascorbic acid and 106.58 ppm activated carbon, and increased as these levels increased. Sugar content was affected more byascorbic acid concentration than by activated carbon level, and the total phenol content was revealed to be the saddle point. In addition, the expected total flavonoid content was maximized with increased levels of ascorbic acid and activated carbon. The data showed that 246-274 ppm (v/v) ascorbic acid and 93-122 ppm (v/v) activated carbon were optimal for juice extraction, and the $R^2$ quality factor for juice processing was significant and within the range of 1-10% for all tested parameters.

• Progress in Medical Physics
• /
• v.3 no.1
• /
• pp.9-18
• /
• 1992

In this study, beta-eucryptite is fabricated and the thermal parameters of this material have been investigated. The thermoluminescence from 4MeV X-ray irradiated beta-eucryptite have been measured over the temperature range of 300K-600K. Thermoluminescence curve from X-ray irradiated beta-eucryptite shows five peaks located at 342K, 392K, 438K, 474K, and 527K. $\tau$, $\delta$ and $\omega$ of peak at 527K are 35K, 39K and 74K, respectively and this peak is found to be 2nd order kinetics. The activation energy of peak shape method is calculated to be 1.03eV and the frequency factor for 527K curve is calculated to be 3.9x10$\^$8/sec$\^$-1/. Based on the various heating rates methods, the activation energy of the peak is computed to be 1.02${\pm}$0.05eV that is similar to 1.19${\pm}$0.03eV of initial rise method. The linearity of thermoluminescence intensity and radiation flux is valid up to 50Gy and beyond higher dose the supralinearity and saturation come out.

• Journal of IKEEE
• /
• v.22 no.3
• /
• pp.613-618
• /
• 2018

In this paper, an active snubber type flyback converter was applied to a 60W class adapter and its test results are reported by producing a prototype. Especially, the prototype of the adapter was designed for both input voltage of 110V and 220V. The output voltage was designed for 60W with 19V class which is the most commonly used in laptops. The prototype was designed to a volume of about $50cm^3$ and a power density of about $1.22W/cm^3$ to minimize the size and verify portability. The maximum efficiency of the prototype was 90.88% at its maximum load of 60W against input voltage change and 91.04% was observed at 60W under input voltage of 220V. Furthermore, the stability of the output voltage against load power was observed to be within about 0.07%.

• Resources Recycling
• /
• v.13 no.4
• /
• pp.32-38
• /
• 2004

Recently, Orimulsion (a bitumen-in emulsion) has received increasing attention as an alternative fuel. Orimulsion combusion produces an ash rich in V, Ni and Mg which are processed to recover metals. As a basic study to recover V from Orimulsion ash, physico-chemcial properties and leaching behaviours were investigated. Orimulsion ash was fine size grains ($d_{50}$ 5.9 $\mu\textrm{m}$) with 16% V, 4 % Ni and 9% S. Vanadium was easily leached in water because Orimulsion ash was mainly constituted of metal sulfates. However, the increase of leaching temperature decreased the extraction percentage of vanadium because of hydrolysis of V(V) to vanadium pentoxide. The addition of sulfuric acid could increase the leaching percentage vanadium. In case of alkaline leaching for selective recovery of vanadium, the oxidzing agent such as $H_2$$O_2$ is required to improve the leaching per-centage

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.1023_1024
• /
• 2009

In this paper, a novel 10kV, 50A, 50kHz pulsed power supply based on IGBT stacks is proposed. Proposed scheme consists of series connected 12 IGBT to generate maximum 10kV output pulse and 10kW full bridge phase-shifted zero voltage switching converter to charge DC capacitor voltage. Each IGBTs are sustain the 830V of capacitor voltage at turn off interval. By turn on the each IGBT for the same time it gives the path for the series connection of charged capacitor. From above turn on and off procedure, high voltage repetitive pulse is applied to the load. The synchronization of gating signal is important of series operation of IGBTs. For gating signal synchronization, specially designed gate power circuit using full bridge inverter and pulse transformer is developed to generate IGBT gating signal.

• Microbiology and Biotechnology Letters
• /
• v.27 no.5
• /
• pp.370-377
• /
• 1999

Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow ad produce xylanase at the culture temperature of 5$0^{\circ}C$. The maximum xylanase production was obtained when 1%(w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH8.0 to 9.0 and from 50 to 6$0^{\circ}C$ and the enzyme was highly heat-stable, whose activity remained was over 50% at 8$0^{\circ}C$, and was quite stable from pH5.0 to 10.0.

• Bulletin of the Korean Mathematical Society
• /
• v.50 no.3
• /
• pp.993-1005
• /
• 2013

In this paper, to include more generalized cases, the authors present a modified concept for the tripled and quadruple fixed point of the mixed monotone mappings. Also, they investigate the existence and uniqueness of fixed point of the ordered monotone operator with the Matkowski contractive conditions in the partial ordered metric spaces. As the direct consequences, the existence of coupled fixed point, tripled fixed point and quadruple fixed point are explored at the common framework and some previous results in [T. G. Bhaskar and V. Lakshmikan-tham, Fixed point theory in partially ordered metric spaces and applications, Nonlinear Anal. 65 (2006), 1379-1393; V. Berinde and M. Borcut, Tripled fixed point theorems for contractive type mappings in partially ordered metric spaces, Nonlinear Anal. 74 (2011), no. 15, 4889-4897; E. Karapinar and N. V. Luong, Quadruple fixed point theorems for nonlinear contractions, Computers and Mathematics with Applications (2012), doi:10.1016/j.camwa.2012.02061] are improved. Finally, some fixed point theorems are proved.