• Title/Summary/Keyword: V$_{}$ 50/

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Virulence of Environmental Urease-Positive and Kanagawa Phenomenon-Negative Vibrio parahaemolyticus

  • Park, Mi-Yeon
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.330-336
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    • 2004
  • Fifty-two pathogenic Vibrio parahaemolyticus strains were isolated from the environments of Busan and Yeosu, Korea. Forty-three of these strains showed protease activities, whereas 4 strains showed $\alpha / \beta$ hemolysin activities and 6 strains had urease activities. Their pathogenic factors were not overlapping except one strain, which had both protease and hemolysin activities. The 6 urease-positive strains (V. parahaemolyticus YKB4, YKB14, S25, YFB20, YFO21, and YFO22) showed the same biochemical characteristics as a reference strain [V. parahaemolyticus KCTC 2471 (urease-negative)], except for urease production. The 6 urease-positive strains showed different urease activities in their culture supernatant during the growth. The urease activity of S25 increased sharply at the late exponential phase, and was the highest at the initial stationary phase and was kept until the late stationary phase. The other 5 isolates, except C25, showed urease activities at the mid-stationary phase and increased steadily until the late stationary phase, when the urease activity was maximal. To compare the degree of virulence of V. parahaemolyticus with different pathogenic factors, hemolysin, protease, or urease-positive strains were injected into groups of 10 each of ICR mice (7- to l0-week-old males). The lethal rates of urease-positive V. parahaemolyticus, YKB14, YKB4, and S25, were significantly high, being 50, 70, and 80%, respectively. Protease-positive V. parahaemolyticus strains FM39 and FM50 showed 40% and 60% of lethal rate, respectively. Hemolysin-positive V. parahaemolyticus strains S34 and S72 had no mortality, similar to nonpathogenic V. parahaemolyticus FM12.

Phase Analysis of Immiscible V-Cu MA Powders by Neutron and X-ray Diffraction (비고용 V-Cu계 MA합금의 중성자 및 X선 회절에 의한 상분석)

  • Lee Chung-Hyo;Cho Jae-Moon;Lee Sang-Jin;Kim Ji-Soon
    • Korean Journal of Materials Research
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    • v.14 no.5
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    • pp.348-352
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    • 2004
  • The mechanical alloying (MA) effect in immiscible V-Cu system with positive heat of mixing was studied by not only the neutron and X-ray diffraction but also the analysis of DSC spectra. The total energy, ΔHt accumulated during MA for the mixture of $V_{50}$ $Cu_{50}$ / powders increased with milling time and approached the saturation value of 14 kJ/mol after 120 h of milling. It can be seen that the free energy difference between the amorphous phase and the pure V and Cu powders with an atomic ratio 5:5 is estimated to be 11 kJ/mol by Miedema et al. This is thermodynamically taken as one of the evidences for the amorphization. The structural changes of V-Cu MA powders were characterized by the X-ray diffraction and neutron diffraction. We take a full advantage of a negligibly small scattering length of the V atom in the neutron diffraction measurement. The neutron diffraction data definitely indicate that the amorphization proceeds gradually but incompletely even after 120 h of MA and bcc-Cu Bragg peaks appears after 60 h of MA.

Numerical analysis of the effect of V-angle on flying wing aerodynamics

  • Zahir Amine;Omer Elsayed
    • Advances in aircraft and spacecraft science
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    • v.10 no.2
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    • pp.141-158
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    • 2023
  • In current research work, the aerodynamics performance of a newly designed large flying V aircraft is numerically investigated. Three Flying V configurations, with V-angles of 50°, 70° and 90° that represent the minimum, moderate, and maximum configurations respectively, were designed and modeled to assess their aerodynamic performance at cruise flight conditions. The unstructured mesh was developed using ICEM CFD and Ansys-Fluent was used as an aerodynamic solver. The developed models were numerically simulated at cruise flight conditions with a Mach number equal to 0.15. K-ω SST turbulence model was chosen to account for flow turbulence.The authors performed steady flow simulations.The results obtained from the experimentation reveal that the maximum main angle configuration of 90° had the highest CLmax value of 0.46 compared to other configurations. While the drag coefficient remained the same for all three configurations, the 50° V-angle configuration achieved the maximum stall angle of 35°. With limited stall delay benefits, the flying V possesses no sufficient stability, due to the flow separation detected at whole elevon and winglet suction side areas at AoA equal and higher than 30°.

Solid Substrate and Submerged Culture Fermentation of Sugar Cane Bagasse for the Production of cellulase and Reducing Sugars by a Local Isolate, Aspergillus terreus SUK-1

  • Wan Mohtar, Yusoff;Massadeh, Muhannad Illayan;Kader, Jalil
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.770-775
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    • 2000
  • Several process parameters were studied to ascertain the effect on degradation of sugar cane bagasse in relation to the production of cellulase enzyme and reducing sugars by Solid Substrate Fermentation (SSF) and Submerged Culture Fermentation (SCF) of Aspergillus terreus SUK-1. The effect of air-flow rate (0-1.3 v/v/m), of different ratios of substrate weight to liquid volume (1:6, 1:10, 1:20, and 1:30 w/v, g/ml), scale-up effect (10, 20, and 100 times of 1:10 ration, w/v) and the effect of temperature (30, 40, 50, and $60^{\circ}C$) in SSF were studied. Air-flow rate of 1.0 v/v/m gave the highest enzyme activity (FPase 0.25 IU/ml, CMCase 1.24 IU/ml) and reducing sugars concentration (0.72 mg/ml). Experiment using 1:10 ratio (w/v) was found to support maximum cellulase activity (FPase 0.58 IU/ml, CMCase 1.97 IU/ml) and reducing sugar concentration (1.23 mg/ml). Scaling-up the ratio of 1:10(w/v) by a factor of 20 gave the highest cellulase activity (FPase 0.71 IU/ml, CMCase 2.25 IU/ml) and reducing sugar concentration (3.67 mg/ml). The optimum temperature for cellulase activity and reducing sugar production was $50^{\circ}C$(FPase 0.792 IU/ml, CMCase 2.25 IU/ml and 3.85 mg/ml for reducing sugar concentration). For SCF, the activity of cellulase enzyme and reducing sugar concentration was found to be lower than that obtained for SSF. The highest cellulase activity obtained in SCF was 50% lower than the highest cellulase activity in SSF, while for reducing sugar concentration, the highest concentration obtained in SCF was 90% lower than that obtained in SSF.

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A Integrated Circuit Design of DC-DC Converter for Flat Panel Display (플랫 판넬표시장치용 DC-DC 컨버터 집적회로의 설계)

  • Lee, Jun-Sung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.10
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    • pp.231-238
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    • 2013
  • This paper describes a DC-DC converter IC for Flat Panel Displays. In case of operate LCD devices various type of DC supply voltage is needed. This device can convert DC voltage from 6~14[V] single supply to -5[V], 15[V], 23[V], and 3.3[V] DC supplies. In order to meet current and voltage specification considered different type of DC-DC converter circuits. In this work a negative charge pump DC-DC converter(-5V), a positive charge pump DC-DC converter(15V), a switching Type Boost DC-DC converter(23V) and a buck DC-DC converter(3.3V). And a oscillator, a thermal shut down circuit, level shift circuits, a bandgap reference circuits are designed. This device has been designed in a 0.35[${\mu}m$] triple-well, double poly, double metal 30[V] CMOS process. The designed circuit is simulated and this one chip product could be applicable for flat panel displays.

A Study on the E-TDLNN Method for the Behavioral Modeling of Power Amplifiers (전력 증폭기의 Behavioral 모델링을 위한 E-TDLNN 방식에 관한 연구)

  • Cho, Suk-Hui;Lee, Jong-Rak;Cho, Kyung-Rae;Seo, Tae-Hwan;Kim, Byung-Chul
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.18 no.10
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    • pp.1157-1162
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    • 2007
  • In this paper, E-TDLNN(Expanded-Tapped Delay Line Neural Network) method is suggested to make the model of power amplifier effectively. This method is the one for making the model of power amplifier through the study in neural network to the target value, the measured output spectrum of power amplifier, after adding the external value factor, gate bias, as an invariant input to the TDLNN method which suggested the memory effect of power amplifier effectively. To prove the validity of suggested method, the data at 2 points, 3.45 V and 3.50 V of gate bias range $3.4{\sim}3.6V$ with the 0.01 V step change, are studied and the predicted results at the gate bias 3.40 V, 3.48 V, 3.53 V and 3.60 V shows good coincidence with the measured values.

Characteristics of Vibrio mimicus K-1 Isolated from Coastal Sea Water (연안 해수에서 분리된 Vibrio mimicus K-1의 특성)

  • KOH Byeong-Ho;LEE Won-Jae;LEE Myung-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.3
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    • pp.292-298
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    • 1994
  • An environmental study was done to examine the distribution of Vibrio mimicus in aquatic environments of Kwangan and Minrak beach, Pusan, Korea. Moreover, both bacteriological characteristics and lethal effects of isolated V. mimicus were observed. Sea water samples were collected monthly from January to September, 1993, and quantitatively analyzed for V. mimicus. This organism was isolated from April(water temperature was $16.3^{\circ}C$), whereas it was not isolated when the water temperature fell below $15^{\circ}C$. V. mimicus counts were not remarkably high, however this study at least describes the distribution and occurrence of the possible highest density in aquatic environments of this region. Among the confirmed V. mimicus strains, the author chose the strongest antibiotic resistant bacterium and named it V. mimicus K-1. This strain has antibiotic resistance to colistin, erythromycin, tetracycline, and penicillin, and most isolates had a higher level of antibiotic resistance than V. mimicus ATCC 33653. The optimum growth for V. mimicus K-1 was observed at $37^{\circ}C$, pH 7.5, and $1\%$ NaCl, respectively. This organism was mostly inactivated by Ultra Violet irradiation (30W, $50^{\circ}C$) for 70 seconds and death lethality increased in proportion to treatment temperature ($D_{50}=5.7min,\;D_{60}=\;2.1min,\;and\;D_{70}=0.7min$).

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Optimization of Tube Voltage according to Patient's Body Type during Limb examination in Digital X-ray Equipment (디지털 엑스선 장비의 사지 검사 시 환자 체형에 따른 관전압 최적화)

  • Kim, Sang-Hyun
    • Journal of the Korean Society of Radiology
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    • v.11 no.5
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    • pp.379-385
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    • 2017
  • This study identifies the optimal tube voltages depending on the changes in the patient's body type for limb tests using a digital radiography (DR) system. For the upper-limp test, the dose area product (DAP) was fixed at $5.06dGy{\ast} cm^2$, and for the lower-limb test, the DAP was fixed at $5.04dGy{\ast} cm^2$. Afterwards, the tube voltage was changed to four different stages and the images were taken three times at each stage. The thickness of the limbs was increased by 10 mm to 30 mm to change in the patient's body type. For a quantitative evaluation, Image J was used to calculate the contrast to noise ratio (CNR) and signal to noise ratio (SNR) among the four groups, according to the tube voltage. For statistical testing, the statistically significant differences were analyzed through the Kruskal-Wallis test at a 95% confidence level. For the qualitative analysis of the images, the pre-determined items were evaluated based on a 5-point Likert scale. In both upper-limb and lower-limb tests, the more the tube voltage increased, the more the CNR and SNR of the images decreased. The test on the changes depending on the patient's body shape showed that the more the thickness increased, the more the CNR and SNR decreased. In the qualitative evaluation on the upper limbs, the more the tube voltage increased, the more score increased to 4.6 at the maximum of 55kV and 3.6 at 40kV, respectively. The mean score for the lower limbs was 4.4, regardless of the tube voltage. The more either the upper or lower limbs got thicker, the more the score generally decreased. The score of the upper limps sharply dropped at 40kV, whereas that of the lower limps sharply dropped at 50kV. For patients with a standard thickness, the optimized images can be obtained when taken at 45kV for the upper limbs, and at 50kV for the lower limbs. However, when the thickness of the patient's limbs increases, it is best to set the tube voltage at 50 kV for the upper limbs and at 55 kV for the lower limbs.

50% Flashover Voltage and V-t Characteristics for $SF_6-N_2$ Mixtures under Positive & Negative Ligtning Impulse Voltage ($SF_6-N_2$ 혼합가스의 정.부극성 뇌충격전압에 대한 50% FOV와 V-t 특성)

  • Song, W.P.;Kim, J.D.;Koh, H.S.;Kim, D.O.;Kwon, Y.H.;Cho, K.B.;Lee, C.H.
    • Proceedings of the KIEE Conference
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    • 1991.07a
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    • pp.300-303
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    • 1991
  • Sulphur-hexafluoride has extensively been used as an insulating gas of electric power equipment in substation for its high dielectric strength, non-toixity and non-flammable properties. But it is very expensive and is liquidizable under the condition of low temperature and high pressure. And it is very sensible to the locally high electric field strength. To resolve these problems, we studied 50% FOV and V-t characteristics of $SF_6-N_2$ mixtures under positive and negative lightning impulses using a 25mm rod-plane gap. 50% FOV of $SF_6-N_2$ 50% mixture was about 85% of pure $SF_6$ and it was known that $SF_6-N_2$ mixtures can be used as an economic substitution insulating gas for $SF_6$.

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Comparative chloroplast genomics and phylogenetic analysis of the Viburnum dilatatum complex (Adoxaceae) in Korea

  • PARK, Jongsun;XI, Hong;OH, Sang-Hun
    • Korean Journal of Plant Taxonomy
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    • v.50 no.1
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    • pp.8-16
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    • 2020
  • Complete chloroplast genome sequences provide detailed information about any structural changes of the genome, instances of phylogenetic reconstruction, and molecular markers for fine-scale analyses. Recent developments of next-generation sequencing (NGS) tools have led to the rapid accumulation of genomic data, especially data pertaining to chloroplasts. Short reads deposited in public databases such as the Sequence Read Archive of the NCBI are open resources, and the corresponding chloroplast genomes are yet to be completed. The V. dilatatum complex in Korea consists of four morphologically similar species: V. dilatatum, V. erosum, V. japonicum, and V. wrightii. Previous molecular phylogenetic analyses based on several DNA regions did not resolve the relationship at the species level. In order to examine the level of variation of the chloroplast genome in the V. dilatatum complex, raw reads of V. dilatatum deposited in the NCBI database were used to reconstruct the whole chloroplast genome, with these results compared to the genomes of V. erosum, V. japonicum, and three other species in Viburnum. These comparative genomics results found no significant structural changes in Viburnum. The degree of interspecific variation among the species in the V. dilatatum complex is very low, suggesting that the species of the complex may have been differentiated recently. The species of the V. dilatatum complex share large unique deletions, providing evidence of close relationships among the species. A phylogenetic analysis of the entire genome of the Viburnum showed that V. dilatatum is a sister to one of two accessions of V. erosum, making V. erosum paraphyletic. Given that the overall degree of variation among the species in the V. dilatatum complex is low, the chloroplast genome may not provide a phylogenetic signal pertaining to relationships among the species.