• Korean Journal of Plant Taxonomy
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• v.49 no.4
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• pp.334-344
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• 2019

The genome size is defined as the amount of DNA in an unreplicated gametic chromosome complement and is expressed as the 1C value. It is a fundamental parameter of organisms that is useful for studies of the genome, as well as biodiversity and conservation. The genome sizes of Korean plants, including Carex (Cyperaceae), have been poorly reported. In this study, we report the genome sizes of 43 species and infraspecific taxa of Korean Carex using flow cytometry, and these results represent about 24.4% of the Carex species and infraspecific taxa distributed on the Korean peninsula. The Plant DNA C-Value Database (release 7.1) updated with and now including our data (a total of 372 Carex accessions) shows that the average genome size of members of the Carex species is 0.47 pg (1C), and the largest genome (C. cuspidate Bertol.; 1C = 1.64 pg) is 8.2 times larger than the smallest (C. brownii Tuck., C. kobomugi Ohwi, C. nubigena D. Don ex Tilloch & Taylor, and C. paxii Kuk.; 1C = 0.20 pg). The large genomes are frequently found in the subgen. Carex, especially in sect. Aulocystis, sect. Digitatae, sect. Glaucae, sect. Paniceae, and sect. Siderostictae. Our data updates the current understanding of genome sizes in Carex. This will serve as the basis for understanding the phylogeny and evolution of Carex and will be especially useful for future genome studies.

• KSBB Journal
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• v.15 no.4
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• pp.337-341
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• 2000

The decanter is very useful to harvest biomass from plant cell cultures in large-scale process. It is very important to obtain high yield and low moisture content in recovered biomass so as to minimize solvent usage in subsequent extraction steps. Effluent clarity was also affected by the differential speed although this affect was more dramatic at higher flow rates than at lower flow rates. Moisure content was largely unaffected by flow rate. A decrease in moisture content was evident as differential speed decreased.

• Journal of Plant Biology
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• v.33 no.2
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• pp.97-104
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• 1990

In order to investigate the host range of Agrobacterium tumefaciens KU12 containing pTi-12, 28 species of dicotyledonous plants were infected with KU12, A136 without Ti plasmid and A348 containing pTi A6, respectively. KU12 and A348 induced tumor in 20 species and 14 species, respectively. This results showed that KU12 has a wide host range. Therefore, it was confirmed that KU12 and pTi-12 are very useful for developing plant vector system having a broad host range.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.586-592
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• 2000

There is currently no satisfactory way to optimize supplemental lighting in a greenhouse-type plant factory especially concerning plant production. In a commercial plant factory, we got outside radiation data, inside radiation data and lamp running data. They have a correlation, but have much disorder. By using regression, tendency between the outside and the inside including supplemental lighting was found. We could estimate the average transmittance of this plant factory. From this estimation, we could admit the amount of inside radiation was supplied as much supplied compared to natural radiation. Then we are trying to investigate of the production amount and the supplemental lighting. Plant factory is environmentally controlled, the temperature and humidity are not actually controlled stable. We propose a design of neural network model could be useful to estimate the profit resulting from the operation of supplemental lighting.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.14 no.1
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• pp.37-43
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• 2005

Plant pipe has a fatigue life which is induced by repeated stress come from the variation of temperature and pressure. To avoid the fatigue crack of plant pipe which is produced by long term repeated stress, plant operator has to limit the mont of operating transient. This paper introduced the study result about discrimination methodology of plant transient by using the fuzzy pattern recognition. As result of applying the fuzzy pattern recognition to actual plant operation data, it is confirmed that fuzzy pattern recognition methodology can be useful for the comparison of similarity for the transients of similar output but has different time pattern.

• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.53-62
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• 2019

The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 mg/L BA with 0.1 mg/L NAA, 0.1, 0.5, 1.0, 2.0 mg/L TDZ with 0.1 mg/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 mg/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 mg/L BA in H. minor (11.1), 1.0 mg/L BA and 0.1 mg/L TDZ in H. venusta (8.1), and 0.5 mg/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.89-103
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• 2011

Two rice varieties, viz. Nonabokra and Pokkali, have been evaluated for their responses to salinity in terms of some physiological and biochemical attributes. During the exposure to salinity (200 mM concentration of sodium chloride for 24, 48, and 72 h), a significant increase in sodium was recorded which was also concomitant with the changes of other metabolic profiles like proline, phenol, polyamine, etc. The protein oxidation was significantly increased and also varied between the two cultivars. The changes in activities of anti-oxidative enzymes under stress were significantly different to the control. The detrimental effects of salinity were also evident in terms of lipid peroxidation, chlorophyll content, protein profiles, and generation of free radicals; and these were more pronounced in Pokkali than in Nonabokra. The assessment and analysis of these physiological characters under salinity could unravel the mechanism of salt responses revealed in this present study and thus might be useful for selection of tolerant plant types under the above conditions of salinity.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.277-283
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• 2015

Plant genome analyses, including Arabidopsis thaliana showed a large gene family of plant receptor kinases with various extracellular ligand-binding domain. Now intensively studies to understand physiological and cellular functions for higher plant receptor kinases in diverse and complex biological processes including plant growth, development, ligands perception including steroid hormone and plant-microbe interactions. Brassinosteroids (BRs) as a one of well know steroid hormone are plant growth hormones that control biomass accumulation and also tolerance to many biotic and abiotic stress conditions and hence are of relevance to agriculture. BRI1 receptor kinase, which is localized in plasma membrane in the cell sense BRs and it bind to a receptor protein known as BRASSINOSTEROID INSENSITIVE 1 (BRI1). Recently, we reported that BRI1 and its co-receptor, BRI1-ASSOCIATED KINASE (BAK1) autophosphorylated on tyrosine residue (s) in vitro and in vivo and thus are dual-specificity kinases. Other plant receptor kinases are also phosphorylated on tyrosine residue (s). Post-translational modifications (PTMs) can be studied by altering the residue modified by directed mutagenesis to mimic the modified state or to prevent the modification. These approaches are useful to not only characterize the regulatory role of a given modification, but may also provide opportunities for plant improvement.

• Journal of Biosystems Engineering
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• v.21 no.3
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• pp.315-324
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• 1996

This study was one of a series of studies on application of machine vision and image processing to extract the geometrical features of plants and to analyze plant growth. Several algorithms were developed to measure morphological properties of plants and describing the growth development of in-situ lettuce(Lactuca sativa L.). Canopy, centroid, leaf density and fractal dimension of plant were measured from a top viewed binary image. It was capable of identifying plants by a thinning top viewed image. Overlapping the thinning side viewed image with a side viewed binary image of plant was very effective to auto-detect meaningful nodes associated with canopy components such as stem, branch, petiole and leaf. And, plant height, stem diameter, number and angle of branches, and internode length and so on were analyzed by using meaningful nodes extracted from overlapped side viewed images. Canopy, leaf density and fractal dimension showed high relation with fresh weight or growth pattern of in-situ lettuces. It was concluded that machine vision system and image processing techniques are very useful in extracting geometrical features and monitoring plant growth, although interactive methods, for some applications, were required.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.200-210
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• 1997

The understanding on the plant genome is accelerated with the fast advance of molecular biological techniques. The molecular dissecting of the plant genome has made possible the precise genotyping the plants, which can be utilized for molecular breeding program. As well, the molecular cloning of genes interested can facilitate the process of gene transfer between intra-and inter-generic taxa. Moreover, the manipulation of the agronomically important QTL genes, which can be rarely performed by the conventional genetic methods, is also possible by the utilization of molecular markers. In addition to these genetical applications, molecular markers are useful in the areas of plant taxonomy and management of germplasm by fingerprinting analysis. This paper describes the theoretical aspects marker technologies and practical applications of each marker technique.