• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.397-397
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2001년도 춘계 학술대회지
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• pp.64-65
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• 2001

1. The cDNA-AFLP was thought to be useful to identify genes associated with early nodulation genes. 2. A total of 37 DNA fragments were found to be differentially expressed between two soybean genotypes. 3. DNA fragments will be sequenced and their function will be identified.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.448-451
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• 2005

This paper presents the methodology to analyze flow duration characteristics and performance prediction for small hydro power(SHP) plants and its application. The flow duration curvecan be decided by using monthly rainfall data at the most of the SHP sites with no useful hydrological data. It was proved that the monthly rainfall data can be characterized by using the cumulative density function of Weibull distribution and Thiessen method were adopted to decide flow duration curve at SHP plants. And, the performance prediction has been studied and development. One SHP plant was selected and performance characteristics was analyzed by using the developed technique. Primary design specfications such as design flowrate, plant capacity, operational rate and annual electricity production for the SHP plant were estimated. It was found that the methodology developed in this study can be a useful tool to predict the performance of SHP plants and candidate sites in Korea.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2005년도 춘계 학술발표대회
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• pp.180-182
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• 2005

• 대한임상검사과학회지
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• 제42권3호
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• pp.116-124
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• 2010

This study was aimed to clerify the cytotoxicity of some plant extracts such as Hosta longissima HONDA (HL), Hemerocallis fulva var. Kwanso REGL (HFVK), Hemerocallis fulva L (HF), Macrocapium officinale NAKAI (MO) and Mentha canadensis var. piperascens HARA (MCVP), the cultured NIH3T3 fibroblasts were treated with 25, 50, 100, 150 and $200{\mu}g/mL$ of five kinds of plant extracts for 48 hours, respectively. The cytotoxicity of plant extracts was measured by MTT and NR assays for the cell viability, and XTT assay for the cell adhesion activity. In this study, HL, MO and FHVK extracts showed the range of midtoxic-non toxic by the criteria of chemical cytotoxicity. While, the HF and MCVP extracts showed midtoxic. In the extract cytotoxicity, HL, MO and FHVK extracts showed non-toxic by the criteria of extract cytotoxicity. While, HF extract was determined as lower-toxic. In the responsive sensitivity of each plant extract on colorimetric assays, HF extract was sensitive to mitochondrial enzyme by MTT assay, lysosomal enzyme by NR assay and mitochondrial nucleus by XTT assay. While, MCVP extract was sensitive to mitochondrial enzyme by MTT assay and lysosomal enzyme by NR assay than other assays. While, HL, HFVK and MO extracts were most sensitive to NR assay. Cell culture is one of useful materials in the screening of cytotoxic and recovary effect on the putative chemical agents or plant extract. And also, colorimetric assay is regarded as very useful tools for quantitative measurement of cytotoxic effect on plant extracts in vitro.

• 생명과학회지
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• 제19권6호
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• pp.809-817
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• 2009

식물에서 유전자의 기능을 연구하는데 있어서 유전자가 과발현 되거나 발현이 억제되는 형질전환체는 해당 유전자의 기능과 관련되어 매우 유용한 정보를 제공한다. 본 연구에서는 modified CaMV 355, UBQ3, UBQ10 프로모터를 pPZP211 벡터에 각각 클로닝 하여 Agrobacterium을 매개로 한 과발현형질전환 식물체 제작에 유용하게 이용할 수 있는 pFGL571, pFGL846, pFGL847을 제조하였다. 이 벡터들은 크기가 작고, 박테리아 내에 high copy로 존재하며, 다중 클로닝 부위에 다양한 제한효소 부위를 가지고 있고, 전체 서열이 알려져 있는 등의 장점을 가지고 있다. GUS 또는 sGFP 리포터 유전자를 포함하는 형질전환 식물체를 제조하여 modified CaMV 35S, UBQ3, UBQ10 프로모터의 활성을 분석한 결과, 세 프로모터 모두 발아 후 대부분의 발달단계와 성숙한 식물체의 꽃 기관에서 높은 활성을 보였다. 한편, 식물에서 유전자 발현 억제에 이용할 수 있는 RNAi 기본 벡터인 pFGL727을 제조하였고, pFGL727을 이용한 벼 RNAi 형질전환체의 분석을 통해 이벡터가 유전자의 발현 억제에 유용하게 이용될 수 있음을 확인하였다. 연구 결과를 종합해 보면, 본 연구에서 제조한 벡터들은 식물에서 유전자 과발현과 발현 억제에 유용하게 이용될수 있을 것으로 기대된다.

• Journal of Plant Biotechnology
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• 제1권1호
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• pp.56-60
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• 1999

Sexual reproduction is an essential process in the propagation of flowering plants. Recent advances in plant cell biology and biotechnology have brought new and powerful methodologies to investigate and manipulate the reproductive processes of angiosperms including agronomically important crop plants. Successful cryopreservation of maize, rye and triticale pollen and young embryos of microspore-and zygote-origine contributes to long term preservation of important plant germ-lines in gene banks. Discovering morphogenetic characteristics of the different developmental pathways taking place in wheat and maize androgenesis in vitro helps to influence the procedure to produce genetically and phenotipically stable homozygous doubled haploid plants for breeding purposes. Detailed ultrastructural and cell-biological studies on the developmental sequences of male and female gametophyte development in wheat, experimental protocols developed to isolate and micromanipulate egg cell protoplasts, make it possible to use plant gametes and the sexual route itself to produce genetically improved organisms. Plant gametes can become useful tools for crop improvement in the near future. Recent achievements by our laboratory in this field are reviewed in the present paper

• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.1-9
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• 1999

Plant biotechnology including plant tissue culture is the most important science to rescue human beings from the crisis of biosphere of the earth which they will face in the 21st century. Global area other than Asia of trangenic crops increased more than 16 times from 1996 to 1998. In Asian countries, micropropagation using tissue culture techniques has been well developed and has contributed to agricultural production. Although researches on levels of laboratory and test field trial of transgenic crops have been performed actively in some Asian countries, areas of growing transgenic crops are still small in Asia countries except in China. In this paper, the status of plant biotechnology in global and Asian countries are reviewed and the future prospect of plant biotechnology in Asia and roles of Korean and Japanese plant scientists in it are discussed.

• Journal of Plant Biotechnology
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• 제41권1호
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• pp.33-37
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• 2014

Lychnis wilfordii (Regel) Maxim is a rare and valued ornamental plant. Germination rate reached 46.6% when seeds were treated with $100mg{\cdot}l^{-1}$ GA (Gibberellic acid). The highest callus induction was observed in the leaf explants of the seedlings on MS medium containing specific concentrations of $0.5mg{\cdot}l^{-1}$ BA ($N^6$-benzyladenine) and $3.0mg{\cdot}l^{-1}$ NAA (a-naphthalene acetic acid). The adventitious shoot was formed in 97.3% of calli on 1/2 WPM (Woody Plant Medium) medium. Shoot elongation of in vitro propagated plantlets was no difference among various medium. The plantlets grew well after transferring to the pot. This in vitro propagation protocol should be useful for conservation of this endangered plant.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 국제학술회의
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• pp.100-113
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• 2005

Leaf color and plant vigor are key indicators of crop health. These visual plant attributes are frequently used by greenhouse managers, producers, and consultants to make water, nutrient, and disease management decisions. Remote sensing techniques can quickly quantify soil and plant attributes, but it requires humans to translate such data into meaningful information. Over time, scientists have used reflectance data from individual wavebands to develop a series of indices that attempt to quantify things like soil organic matter content, leaf chlorophyll concentration, leaf area index, vegetative cover, amount of living biomass, and grain yield. The recent introduction of active sensors that function independent of natural light has greatly expanded the capabilities of scientists and managers to obtain useful information. Characteristics and limitations of active sensors need to be understood to optimize their use for making improved management decisions. Pot experiments involving sand culture were conducted in 2003 and 2004 in a green house to evaluate corn and red pepper biomass. The rNDVI, gNDVI and aNDVI by ground-based remote sensors were used for evaluation of corn and red pepper biomass. The result obtained from the case study was shown that ground remote sensing as a non-destructive real-time assessment of plant nitrogen status was thought to be a useful tool for in season crop nitrogen management providing both spatial and temporal information.