• Plant Breeding and Biotechnology
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• v.5 no.2
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• pp.134-142
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• 2017

Resource plants are important and have strong potential for a variety of utilities as crops or pharmaceutical materials. However, most resource plants remain wild and thus their utility for breeding and biotechnology is limited. Molecular markers are useful to initiate genetic study and molecular breeding for these understudied resource plants. We collected various wild collections of Peucedanum japonicum which is indigenous resource plants utilized as oriental medicine and leafy vegetables in Korea. In this study, we produced two independent whole genome sequences (WGSs) from two collections and identified large scale polymorphic simple sequence repeat (pSSR) based on our pipeline to develop SSR markers based on comparison of two WGSs. We identified a total of 452 candidate pSSR contigs. To confirm the accuracy and utility of pSSR, we designed ten SSR primer pairs and successfully applied those to seven collections of P. japonicum. The WGS and pSSR candidates identified in this study will be useful resource for genetic research and breeding purpose for the valuable resource plant, P. japonicum.

• Journal of Plant Biology
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• v.9 no.1_2
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• pp.22-24
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• 1966

1. Number of accessory chromosomes in the progenies of rye which were obtained from mother plants having 2, 4, and 6 accessory chromosomes by open pollination were observed. The result was shown in the Table 1. 2. Cross between the plant with 14 ordinary chromosomes having chromsome breakage and normal plant with 14 ordinary chromosomes was carried out. A plant among the progeny of this cross was found to have two accessory chromosomes in addition to 14 ordinary chromosomes. This phenomenon may be useful to postulzte the possible origin of accessory chromosome in rye.

• Journal of Plant Biology
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• v.28 no.4
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• pp.329-332
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• 1985

A simple new device for obtaining very clear epidermal imprints for light microscopic studies is discussed. This new device is developed from“Britfix”(polystyrene cement) which is non-toxic to the plant organs. It involves direct application of the material on the desired surface of the plant organ to obtain thin, transparent replica. From the present investigation“Britfix”is found to be useful for the study of epidermal anatomy, morphology and physiology. Epidermal imprints can be mounted on the microscope slide without a mounting medium. Permanent slide of these imprints can be kept for any desired period without any deterioration of the replica.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.99-104
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• 2013

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amplified the targets, and gradient PCRs indicated that the optimal temperature for the 14 useful primer sets was $51.9^{\circ}C$. Some primer sets worked well regardless of annealing temperature while others required a very specific annealing temperature. A primer specificity test using plant total RNAs and cDNAs of other plant virus-infected samples demonstrated that the designed primer sets were highly specific and generated reproducible results. The newly developed RT-PCR primer sets would be useful for quarantine inspections aimed at preventing the entry of exotic plant viruses into Korea.

• Current Research on Agriculture and Life Sciences
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• v.31 no.2
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• pp.75-82
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• 2013

Haploids are plants with a gametophytic number of chromosomes in their sporophytes. Androgenesis occurs from asymmetric division of pollen grains into generative cells and vegetative cells, followed by re-entry of the vegetative cell during S-phase, which causes microspores progress into G2/M transition in culture. One of the most interesting features of haploids is the possibility to produce doubled haploid (DH) individuals. Doubled haploidy is extremely useful to plant breeders because it enables shortened breeding periods and efficiency in selection of useful recessive agronomic traits. Doubled-haploid technology is not only applicable to breeding, but also to transformation programs of desired genes. In addition to practical breeding programs, DH lines provide useful materials of fundamental genetics including exploitation of QTLs and genes conferred with various agronomic traits by establishing DH populations. This paper provides historical overviews on androgenesis and describes several mechanisms associated with pollen embryogenesis, including mode of actions in pollen embryogenesis, mechanisms of chromosome doubling and factors affecting androgenesis. We also discuss recent progress in application of haploids to breeding, genes associated with in vitro response and drawbacks to anther culture for application of doubled haploids in crop breeding.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.32-56
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• 2005

This study aims to investigate the distribution of the useful resources plants and the flora of Deogyusan. The flora of Deogyusan was investigated from April in 2002 to November in 2003. Vascular plants of this area consist of total 549 taxa; 9 forma, 64 varieties, 476 species, 281 genera, 90 families. It corresponds to $13.5\%$ of totally 4,071 taxa appeared in Korea. Useful plants of this area were investigated 399 taxa. It corresponds to $72.5\%$ of collected plants in this area under our study. Among the useful plants, there are 205 taxa of edible source, 179 taxa of pasture source, 159 taxa of medicinal source, 70 taxa of ornamental source, 15 taxa of timber, and 17 taxa of industrial raw materials. Korean endemic plants were composed 18 families, 23 genera, 19 species, 4 varieties and totaling 25 taxa.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.49 no.9
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• pp.504-511
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• 2000

The reliability of system is to become a important concern in developed industry. The controller based on the reliability is so important position. PPC(Plant Protection Controller) is for plant protection and human life by fault detection and control action against the transient condition of plant. The protection system of the nuclear reactor and chemical reactor are representative of PPC. This paper presents analysis of PPC relaibility formal problem statement of optimal redundancy based on the reliability for PPC. And the problem is optimized by genetic algorithm, The genetic algorithms is useful algorithm in case of large searching complex gradient existence local minimum. The genetic algorithms is useful algorithm is case of large searching complex gradient existence local minimum. The ability and effectiveness of the proposed optimization is demonstrated by the target reliability of one channel. PPC. using the failure rate based on the MIL-HDBK-217

• Korean Journal of Plant Resources
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• v.23 no.3
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• pp.256-260
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• 2010

We performed the floristic study of Carpathian mountains in Ukraine from April 2008 till October 2009. We designated eight important areas to investigate in Ukraine Carpathians including Svydovets, Chornohora, Kuziy-Trybushany, Maramorosh, Uholka-Shyrokyi Luh, Narcissi Valley, Chorna Hora, and Yulivska Hora. The total numbers of vascular plants in investigated area were 1,349 speices from 502 genera and 112 families. The endemic species to Carpathians numbered to 65 from 49 genera belonging to 24 families, and it was corresponded to 4.8% of the 1,349 vascular species. We prepared the list of vascular plants with compiling database on useful plants. Ukraine Carpathians endemic species could be applied as high value products in research and practice. Moreover, the flora of Carpathian mountains which is distributed in five nations in eastern Europe and famous for Primeval Beech Forests (Fagus sylvatica), is useful to compare the floristic study with Korean flora.

• Korean Journal Plant Pathology
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• v.10 no.1
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• pp.1-6
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• 1994

Randomly chosen recombinant clones of Fusarium oxysporum were analysed to select useful probes for relatedness analysis of Fusarium oxysporum. Genomic DNA of F. oxysproum f. sp. cubense, digested with HindIII, was ligated to pUC118 and used to transform Escherichia coli strai DH5$\alpha$. Three clones were identified that hybridized to mutiple restriction fragments of some formae speciales of F. oxysporum. These probes detected repetitive sequences in HindIII or EcoRI digested DNAs. Repeated copy clone pFC46, pFC52 and pFC54 showed evident polymorphisms among ten formae speciales of this fungus. Since clone pFC 52 strongly hybridized to multiple EcoRI-digested restriction fragments of f. sp. cubense, it may be useful as a probe for analysis of other genetic characteristics of this forma specialis. The results suggest that our clones might be very useful as probes for relatedness analysis between or within formae speciales of Fusarium oxysporum.

• Journal of Ginseng Research
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• v.30 no.3
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• pp.153-157
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• 2006

This study was carried out to identify the age of red ginseng using the stem vestige counting methods and annual ring staining method. The samples were soaked, humidified, and restored before identifying the age. Root tissue was removed from rhizome after soaking treatment in $50^{\circ}C$ water for three days. It was found to be useful for precise identification of age. Safranine staining for counting the annual ring in sliced ginseng was not useful due to the poor staining. However, annual ring was clearly revealed whenr humidified sliced ginseng or soaked sliced ginseng were dried mechanically. These two method was useful to identify the age of red ginseng.