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Association between SNPs within Prolactin Gene and Milk Performance Traits in Holstein Dairy Cattle

  • He, Feng;Sun, Dongxiao;Yu, Ying;Wang, Yachun;Zhang, Yuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.10
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    • pp.1384-1389
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    • 2006
  • Prolactin plays an important role in mammary gland development, milk section initiation and maintenance of lactation, so the bovine prolactin gene is considered as a potential quantitative trait locus affecting milk performance traits in dairy cattle. In this study, to determine the association between prolactin and milk performance traits, the genetic polymorphisms of a part of the prolactin gene were detected in a population of 649 cows of Chinese Holstein Dairy Cattle. Three SNPs in the promoter and one SNP in the intron1 of prolactin were identified, which was A/C (-767), G/T (-485), C/A (-247), and C/T (427), respectively. Statistical results indicated that one of SNP within promote, CHBP2, was significantly associated with milk yield (p<0.01), fat yield (p<0.05), protein yield (p<0.01), and protein percentage (p<0.05). The cows with genotype BB of CHBP2 had significantly higher milk yield (p<0.01), fat yield (p<0.05), and protein yield (p<0.01) than those of cows with genotype AA, while cows with genotype AA showed the highest protein percentage (p<0.05). In addition, based on the nine major haplotypes constructed from the four SNPs, the association analysis between diplotypes and milk performance trait was carried out. Results showed that the least square mean for fat yield of diplotype H2H8 was significantly higher than those of other eleven diplotypes (p<0.05). Our findings implied that CHBP2 and H2H8 of prolactin would be useful genetic markers in selection program on milk performance traits in Holstein Dairy Cattle.

A Study on Extendable Instruction Set Computer 32 bit Microprocessor (확장 명령어 32비트 마이크로 프로세서에 관한 연구)

  • 조건영
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.36D no.5
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    • pp.11-20
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    • 1999
  • The data transfer width between the mocroprocessor and the memory comes to a critical part that limits system performance since the data transfer width has been as it was while the performance of a microprocessor is getting higher due to its continuous development in speed. And it is important that the memory should be in small size for the reduction of embedded microprocessor's price which is integrated on a single chip with the memory and IO circuit. In this paper, a mocroprocessor tentatively named as Extendable Instruction Set Computer(EISC) is proposed as the high code density 32 bit mocroprocessor architecture. The 32 bit EISC has 16 general purpose registers and 16 bit fixed length instruction which has the short length offset and small immediate operand. By using and extend register and extend flag, the offset and immediate operand could be extended. The proposed 32 bit EISC is implemented with an FPGA and all of its functions have been tested and verified at 1.8432MHz. And the cross assembler, the cross C/C++ compiler and the instruction simulator of the 32 bit EISC shows 140-220% and 120-140% higher code density than RISC and CISC respectively, which is much higher than any other traditional architectures. As a consequence, the EISC is suitable for the next generation computer architecture since it requires less data transfer width compared to any other ones. And its lower memory requirement will embedded microprocessor more useful.

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Perfomance Analysis for the IPC Interface Part in a Distributed ATM Switching Control System (분산 ATM 교환제어시스템에서 프로세서간 통신 정합부에 대한 성능 분석)

  • Yeo, Hwan-Geun;Song, Kwang-Suk;Ro, Soong-Hwan;Ki, Jang-Geun
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.35S no.6
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    • pp.25-35
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    • 1998
  • The control system architecture in switching systems have undergone numerous changes to provide various call processing capability needed in telecommunication services. During call processing in a distributed switching control environment, the delay effect due to communication among main processors or peripheral controllers is one of the limiting factors which affect the system performance. In this paper, we propose a performance model for an IPC(Inter Processor Communication) interface hardware block which is required on the ATM cell-based message processing in a distributed ATM exchange system, and analyze the primary causes which affect the processor performance through the simulation. Consequently, It can be shown that the local CPU of the several components(resources) related to the IPC scheme is a bottleneck factor in achieving the maximum system performance from the simulation results, such as the utilization of each processing component according to the change of the input message rate, and the queue length and processing delay according to input message rate. And we also give some useful results such as the maximum message processing capacity according to the change of the performance of local CPU, and the local CPU maximum throughput according to the change of average message length, which is applicable as a reference data for the improvement or expansion of the ATM control system.

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Implementation of High-Throughput SHA-1 Hash Algorithm using Multiple Unfolding Technique (다중 언폴딩 기법을 이용한 SHA-1 해쉬 알고리즘 고속 구현)

  • Lee, Eun-Hee;Lee, Je-Hoon;Jang, Young-Jo;Cho, Kyoung-Rok
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.47 no.4
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    • pp.41-49
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    • 2010
  • This paper proposes a new high speed SHA-1 architecture using multiple unfolding and pre-computation techniques. We unfolds iterative hash operations to 2 continuos hash stage and reschedules computation timing. Then, the part of critical path is computed at the previous hash operation round and the rest is performed in the present round. These techniques reduce 3 additions to 2 additions on the critical path. It makes the maximum clock frequency of 118 MHz which provides throughput rate of 5.9 Gbps. The proposed architecture shows 26% higher throughput with a 32% smaller hardware size compared to other counterparts. This paper also introduces a analytical model of multiple SHA-1 architecture at the system level that maps a large input data on SHA-1 block in parallel. The model gives us the required number of SHA-1 blocks for a large multimedia data processing that it helps to make decision hardware configuration. The hs fospeed SHA-1 is useful to generate a condensed message and may strengthen the security of mobile communication and internet service.

Detecting near-duplication Video Using Motion and Image Pattern Descriptor (움직임과 영상 패턴 서술자를 이용한 중복 동영상 검출)

  • Jin, Ju-Kyong;Na, Sang-Il;Jenong, Dong-Seok
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.48 no.4
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    • pp.107-115
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    • 2011
  • In this paper, we proposed fast and efficient algorithm for detecting near-duplication based on content based retrieval in large scale video database. For handling large amounts of video easily, we split the video into small segment using scene change detection. In case of video services and copyright related business models, it is need to technology that detect near-duplicates, that longer matched video than to search video containing short part or a frame of original. To detect near-duplicate video, we proposed motion distribution and frame descriptor in a video segment. The motion distribution descriptor is constructed by obtaining motion vector from macro blocks during the video decoding process. When matching between descriptors, we use the motion distribution descriptor as filtering to improving matching speed. However, motion distribution has low discriminability. To improve discrimination, we decide to identification using frame descriptor extracted from selected representative frames within a scene segmentation. The proposed algorithm shows high success rate and low false alarm rate. In addition, the matching speed of this descriptor is very fast, we confirm this algorithm can be useful to practical application.

A molecular systematic study of Korean Iris (Iridaceae) based on RAPD analysis (RAPD에 의한 한국산 붓꽃속(Iris)의 계통분류학적 연구)

  • Park, Seon-Joo;Sim, Jeong-Ki;Park, Hong-Duok
    • Korean Journal of Plant Taxonomy
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    • v.32 no.4
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    • pp.383-396
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    • 2002
  • RAPD analyses were compared for 17 taxa of Korean Iris including the subgenus Sisyrinchium and Belamcanda. Eighty scorable RAPD markers were formed from the PCR reactions using 10 random oligoprimers. In this systematic analyses which used neighbor-joining methods including bootstrapping analyses with genetic coefficients, the Korean Iris were divided into three subgenera (Limniris, Crossiris, Pardanthopsis), or two genera (Limniris, Pardanthopsis). The molecular data agree with the previous classification system that recognized two sections and six series for the subgenus Limniris because the subgenus is comprised of four clades in the RAPD analyses. According to the molecula data, the series Chinensis should be divided into two groups. The minutoaurea group is composed of I. koreana, I. odaesanensis, and I. minitoaurea, while the rossi group is comprised of two varieties of I. rossi. The series Tripetalae is closely allied with the series Sibiricae, whereas the series Ensatae is recognized as a sister group to the series Ruthencae. The molecular phylogeny, which was based on RAPD analysis, for the most part agreed with the data proposed by previous authors. This is because the basis of morphological and ITS sequence data suggests that the RAPD markers should be very useful in addressing phylogenetic questions about the genus Iris.

Analytical Method Development of Avicularin and Quercitrin in Astilbe chinensis Extract Using HPLC (HPLC를 이용한 노루오줌 추출물의 Avicularin 및 Quercitrin 분석법 개발)

  • Kwon, Jin Gwan;Jung, Yeon Woo;Seo, Changon;Hong, Seong Su;Shin, Hyun Tak;Jung, Su Young;Choi, Jeong June;Kim, Jin Kyu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.4
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    • pp.333-340
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    • 2019
  • This study attempted to eatablish a high performance liquid chromatography (HPLC) analysis method for the determination of avicularin, quercitrin as a part of the quality control for the development of functional cosmetic materials from Astilbe chinensis extract. HPLC was performed on a Capcell Pak C18 MGII column with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at 30 ℃. The analyte was detected at 254 nm. The HPLC method was performed in accordance with the international conference on harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation of avicularin and quercitrin were 0.094 and 0.285 mg/mL, 0.031 and 0.095 mg/mL respectively. Calibration curves showed good linearity r2 > 0.99990 for avicularin and r2 > 0.99994 for quercitrin. Precision of analysis was satisfied with less than 0.59% for avicularin and 0.63% for quercitrin. Recoveries of quantified compounds ranged from 100.97 to 101.77% for avicularin and 100.18 to 100.32% for quercitrin. These result indicated that the established HPLC method is very useful for the determination of marker compounds in A. chinensis extracts.

Anti-apoptotic Activity of Ginsenoside Rb1 in Hydrogen Peroxide-treated Chondrocytes: Stabilization of Mitochondria and the Inhibition of Caspase-3

  • Na, Ji-Young;Kim, Sok-Ho;Song, Ki-Bbeum;Lim, Kyu-Hee;Shin, Gee-Wook;Kim, Jong-Hoon;Kim, Bum-Seok;Kwon, Young-Bae;Kwon, Jung-Kee
    • Journal of Ginseng Research
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    • v.36 no.3
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    • pp.242-247
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    • 2012
  • Chondrocyte apoptosis has been recognized as an important factor in the pathogenesis of osteoarthritis (OA). Hydrogen peroxide ($H_2O_2$), which produces reactive oxygen species, reportedly induces apoptosis in chondrocytes. The ginsenoside $Rb_1$ (G-$Rb_1$) is the principal component in ginseng and has been shown to have a variety of biological activities, such as anti-arthritis, anti-inflammation, and anti-tumor activities. In this study, we evaluated the effects of G-$Rb_1$ on the mitochondrial permeability transition (MPT) and caspase-3 activity of chondrocyte apoptosis induced by $H_2O_2$. Cultured rat articular chondrocytes were exposed to $H_2O_2$ with or without G-$Rb_1$ and assessed for viability, MPT, Bcl-xL/Bax expression, caspase-3 activity, and apoptosis. The co-treatment with G-$Rb_1$ showed an inhibition of MPT, caspase-3 activity, and cell death. Additionally, the levels of the apoptotic protein Bax were significantly lower and the levels of the anti-apoptotic protein Bcl-xL were higher compared with $H_2O_2$ treatment alone. The results of this study demonstrate that G-$Rb_1$ protects chondrocytes against $H_2O_2$-induced apoptosis, at least in part via the inhibition of MPT and caspase-3 activity. These results demonstrate that G-$Rb_1$ is a potentially useful drug for the treatment of OA patients.

Differentiation of Border Cells During Oogenesis in Drosophila melanogaster (노랑초파리 난자 형성과정 동안의 경계세포의 분화)

  • Gye, Myung-Chan;Cho, Kyoung-Sang;Lee, Chung-Choo
    • Development and Reproduction
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    • v.2 no.1
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    • pp.45-52
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    • 1998
  • An enhncer detector line(EDL) having P[1ArB] insertion in X chromosome with expression of reporter gene (lacZ) in the polar cells and border cell of egg chamber was established and used to monitor the differentiation and migration of border cells during the oogenesis of Drosophila. differentiation of border cell from the anterior polar follicle cells was evident in stage-9 egg chamber of EDL149 which was characterized by migration of columnar follicle cells toward posterior of egg chamber surrounding the oocyte. Migration of border cells was observed in the stage-9 and -10 egg chambers. \beta -galactosidase activities were rapidly increased during the first 4 days after eclosion, and it coincided with the timing of border cell differentiation in the ovary during adult life. Homozygote of EDL149 showed some retardation of border cell migration , resulting absence of migration of some border cells in the anterior part of egg chamber or delayed migration of some border cells in the stage-10 egg chamber. These results suggest that the P[1ArB] of EDL149 is inserted at the locus of the structural gene required for the border cell migration. In addition to the expression in egg chambers, lacZ expression was also detected in the meiotic germ cells of testis and antenna, suggesting the possible requirement of the trapped gene function in these organ. this EDL and enhancer trapped gene might be useful for the study of developmentally regulated cell migration.

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Estimation of the relationship between below-ground root and above-ground canopy development by measuring dynamic change of soil ammonium-N concentration in rice

  • Fushimi, Erina;Yoshida, Hiroe;Tokida, Takeshi;Nakagawa, Hiroshi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.183-183
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    • 2017
  • In the early part of rice growth, root volume primarily limits the amount of plant-accessible nitrogen (N). Therefore, knowledge of the root development is important for modeling N uptake of rice. The timing when the volume of rhizosphere cover the whole soil is also important to carry out timely top dressing. However, information about initial root expansion and associated N uptake is limited due to intrinsic technical difficulties in assessing below-ground processes. Some studies, however, showed a close relationship between below-ground root and above-ground leaf development, suggesting a possibility that above-ground attributes could serve as surrogates for the root processes. In this study, we investigated the relationship between below-ground and above-ground development of rice. Field experiments were conducted where we cultivated Koshihikari (a leading cultivar in Japan) for four different cropping schedules in 2012. In 2016, Gimbozu (HEG4) and three flowering time mutant lines of Gimbozu (X61 (se13), HS276 (ef7), DMG9 (se13, ef7)) were examined for a single season. Experiments were performed with three replications in a completely randomized design. We monitored ammonium-N concentration ([NH4+-N]) in soil solution by repeatedly taking samples from a porous tubing (10-cm long) vertically inserted at the most distant point from surrounding rice hills. Samples were taken in triplicate (= triplicate tubes) and every three days from transplanting in each experimental unit. For above-ground attributes, leaf area index (LAI) was measured in 2012, whereas soil coverage ratio was estimated by image processing in 2016. Results showed that [NH4+-N] increased gradually after transplanting and then rapidly decreased from a certain day. This distinct drop in [NH4+-N] informed us the timing at which the rice root system reached the point of porous tubing and thus essentially covered the whole soil volume. The LAI at the dropping point was about 0.43 regardless of the cropping schedules in 2012 experiment. In 2016, the coverage ratio at the N dropping point was within the range of 0.12 to 0.19 for four genotypes having different growth durations. In addition, the coverage ratios at seven weeks after the transplanting showed a good correspondence to LAI across the four genotypes. We therefore conclude that both LAI and coverage ratio may serve as robust indicators for root development and might be useful to estimate the timing when the root system fully cover the soil volume. Results obtained here will also contribute to develop models that can predict not only above-ground canopy development but also associated below-ground processes.

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