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Purification and Utilization of Industrial Waste Water Using Microorganism -(Part 1) Isolation of the yeast strain from organic waste water and its use on waste water treatment- (산업폐수의 처리 및 이용에 관한 연구 -(제 1 보) 효모균주의 분리와 이에 의한 유기성폐수의 처리에 관하여-)

  • Lee, Kang-Heup;Yim, Sung-Sam;Park, Tai-Won
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.228-235
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    • 1977
  • The yeast strain was isolated from food industry waste water and its identification and biological characteristics were investigated. The optimum condition for cultivations and its activities for the reduction of B.O.D. on the food industry waste water were also confirmed. The results are as follows; 1) The isolated was identified as Candida curvata. 2) Candida curvata grew well in all of the experimented media, so and it can be regarded as a useful strain in the treatment of food industry waste water. 3) There was only a slight difference in the induction period between sterilized cultivation and unsterilized cultivation. But in the ice cream waste water, the period was considerably longer in unsterilized cultivation. 4) Specific rate of growth of Candida curvata in sugar waste water was 0.50/hr, ice cream waste water 0.50/hr, and beer waste water 1.0/hr. 5) Increasing of innoculum reduced the induction period in unsterilized cultivation. 6) The amount of dried yeast from sugar waste water were $175mg/{\ell}$, ice cream waste water $628mg/{\ell}$, and beer waste water $857mg/{\ell}$. Crude protein content in the dried yeast from sugar waste water were 52%, ice cream waste water 54%, and beer waste water 54%. 7) The rate of BOD reduction in sugar waste water were 49%, ice cream waste water 80%, and beer waste water 64%.

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Biological Function of Single Chain Glycoprotein Hormone Mutants

  • Min, Kwan-Sik;Chang, Yoo-Min;Chang, Sun-Hwa;Lee, Hyen-Gi;Lee, Yun-Gun;Chang, Won-Kyong;Cheong, Il-Cheong
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.54-54
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    • 2001
  • Human chorionic gonadotropin (hCG) is a member of the glycoprotein hormone family which includes FSH, hCG, TSH. These hormone family is characterized by a heterodimeric structure composed a common $\alpha$-subunit noncovalently linked to a hormone specific $\beta$-subunit. The correct conformation of the heterodimer is also important for efficient secretion, hormone-specific post-translational modifications, receptor binding and signal transduciton. To determine $\alpha$ and $\beta$-subunits can be synthesized as a single polypeptide chain (tethered-hCG) and also display biological activity, the tethered-hCG molecule by fusing the carboxyl terminus of the hCG $\beta$-subunit to the amino terminus of the $\alpha$-subunit was constructed and transfected into chinese hamster ovary (CHO-K1) cells. We also constructed C-terminal deletion mutants (D9l, D89, D88, D87, D86, D84, D83) of single chain hCG to determine the biological function (secretion, LH-activity, receptor binding, cAMP production) of these mutants. Between six and eight stably transfected pools of cells expressing wild type and mutant hCGs were selected for neomycin resistant. The hCGs secreted by the stably transfected cells into serum-free media were collected and quantified by radioimmunoassay, as described in protocol (DPC(hCG IRMA). LH activity was in terms of testosterone production and aromatase activity in primary cultured rat Leydig cells. The tethered-wthCG was efficiently secreted and showed similar LH-like activity to the dimeric hCG. The D83hCG mutant was not detected in this assay. It is suggest that hCG C-terminal part is very important for hCG secretion. Now, we checking the LH-like activity of these mutant hCGs. These data indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion.

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A Design and Implementation of the Management Sever for the Gateway Supporting Home Networking Using the UML (UML을 이용한 흠 네트워킹 지원 게이트웨이 관리 서버 설계 및 구현)

  • 권진혁;민병조;강명석;남의석;김학배
    • Journal of the Korea Computer Industry Society
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    • v.5 no.3
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    • pp.393-404
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    • 2004
  • Recently, public home have used a more than two computer connected with network, and several home appliances using independently with internet or network are developing to be related closely with the network. Therefore, the home utilized for a simple terminal of the global network in the past is being expanded to another part of the sub network. For a variety of connecting home-area protocols with the existing existing network, we require a new Residential Gateway(RG) that does not only make the home-area network operating in the sub network but also connects to the external network. In this paper, RG has intrinsic limits against flexible service due to IP address assignment and hardware capacity. In order to solve this problem in the RG, we propose a Management Server(MS). The MS that offers the integrated managements and control services for a variety of devices connected the RG in the home-area. It can not only solve the dynamic IP address assigning problem but also assigns private IP addresses to the home network devices through the Network Address Translation(NAT). It also provides somewhat useful functions for the home network and the RG for other additional services. <중략> The MS is using a SNMP protocol for managing the RG in the domain, a polling method of the MS and the RG compose a sequence polling method, a polling method using a multi-process and a multi-thread. In this paper, we introduce a problem with polling method separately, show a polling method between the MS and the RG using a multi-thread.

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Influence of the Fire on Emergency Evacuation Support System (대공간용 비상피난지원 시스템에 화재가 미치는 영향 분석)

  • Kim, JiTae;Sung, Kun Hyuk;Park, Won Hee;Lee, Duck Hee;Woo, Jun You;Ro, Kyoung-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.5
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    • pp.626-631
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    • 2018
  • An emergency evacuation support system is used to maintain evacuation routes by pressurizing a space inside screens. In cases of fire, it is important to understand the thermal distributions in the tunnel for preventing system failure. In this study, we numerically investigated the effect of fire on an emergency evacuation support system in a large fabric store with some fire scenarios with different combustibles. The critical temperature for system failures was assumed to be $200^{\circ}C$. As a result, the highest temperature was predicted in the ceiling part due to the effect of a ceiling jet, and the fire safety of the screen was secured at distances of 20 to 30 m according to the heat release rate. To prevent the inflow of smoke into the system, it is necessary to maintain more than 5 Pa if positive pressure inside the smoke screen. The results of this study could be useful for designing an emergency evacuation support system.

Analysis on Kindergarten Teachers' Stage of Concerns about Software Education: An Application of the Concerns-Based Adoption Model(CBAM) (유치원 교사의 소프트웨어 교육에 대한 관심도 분석: 관심중심수용모형(CBAM)을 중심으로)

  • Park, Sun-Mi;Jung, Ji-Hyun;Kang, Min-Jeng
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.462-471
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    • 2018
  • Even though software (SW) education has not been considered part of the current national kindergarten curriculum, in practice there is growing interest in adopting it. Teachers would be expected to play a key role in the successful introduction and implementation of new educational changes, such as SW education. In preparation for its adoption in ECE, it would be useful to determine ECE teachers' perception and attitudes toward early childhood software education. For this study, 219 ECE teachers' level of concern toward SW education was surveyed using the Stages of Concern Questionnaire. It was found that the teachers' level of concern was the highest at stage 0-Unconcerned and that they had high levels of stage management, personal and informational concern. Thus, a non-user pattern was mostly indicated. However, compared to the typical non-user pattern, the pattern aspect close to critical non-user ever appeared to some extent. In addition, a significant difference in the level of concern was shown at all stages depending on the awareness of the necessity for SW education. The teachers with SW training experience showed a higher intensity only at stage 0. There was a statistically significant difference in stages 0 and 6 depending on the future implementation decision. These results will be utilized as a resource in building an ECE teachers' support system according to their level of concern about SW education.

Raising Visual Experience of Soccer Video for Mobile Viewers (이동형 단말기 사용자를 위한 축구경기 비디오의 시청경험 향상 방법)

  • Ahn, Il-Koo;Ko, Jae-Seung;Kim, Won-Jun;Kim, Chang-Ick
    • Journal of KIISE:Computing Practices and Letters
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    • v.13 no.3
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    • pp.165-178
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    • 2007
  • The recent progress in multimedia signal processing and transmission technologies has contributed to the extensive use of multimedia devices to watch sports games with small LCD panel. However, the most of video sequences are captured for normal viewing on standard TV or HDTV, for cost reasons, merely resized and delivered without additional editing. This may give the small-display-viewers uncomfortable experiences in understanding what is happening in a scene. For instance, in a soccer video sequence taken by a long-shot camera techniques, the tiny objects (e.g., soccer ball and players) may not be clearly viewed on the small LCD panel. Moreover, it is also difficult to recognize the contents of the scorebox which contains the elapsed time and scores. This renuires intelligent display technique to provide small-display-viewers with better experience. To this end, one of the key technologies is to determine region of interest (ROI) and display the magnified ROI on the screen, where ROI is a part of the scene that viewers pay more attention to than other regions. Examples include a region surrounding a ball in long-shot and a scorebox located in the comer of each frame. In this paper, we propose a scheme for raising viewing experiences of multimedia mobile device users. Instead of taking generic approaches utilizing visually salient features for extraction of ROI in a scene, we take domain-specific approach to exploit unique attributes of the soccer video. The proposed scheme consists of two modules: ROI determination and scorebox extraction. The experimental results show that the proposed scheme offers useful tools for intelligent video display on multimedia mobile devices.

Studies on Microbial Penicillin Amidase (Part 6) Immobilization of Penicillin Amidase from Bacillus megaterium by Adsorption and Acrylamide Gel Entrappment (미생물 페니실린 아미다제에 관한 연구 (제 6 보) 흡착효소의 아크릴아마이드젤 포괄방법에 의한 Bacillus megaterium의 변이주가 생산하는 페니실린 아미다제의 고정화에 관한 연구)

  • Seong, Baik-Lin;Son, Hyeung-Jin;Mheen, Tae-Ick;Moon H. Han
    • Microbiology and Biotechnology Letters
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    • v.9 no.4
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    • pp.197-205
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    • 1981
  • Penicillin amidase of Bacillus megaterium was recovered from the fermentation broth by adsorption on celite and immobilized by entrapping the adsorbed enzyme in acrylamide gel. The operational stability in column reactor was greatly increased by entrappment as compared with that of without entrappment. The optimum pH of the immobilized enzyme was 8.7 with broader activity profile than that of the free enzyme, while the most stable pH range appeared to be between pH 7.5 and 8.0. The optimum temperature was shifted to 5$0^{\circ}C$ from 45$^{\circ}C$ for the soluble enzyme. The values of Km and the inhibition constants for 6-APA( $K_{ia}$ ) and phenylacetic acid ( $K_{ip}$ ), were 4.55 mM, 36.5mM, and 10.5mM, respectively. No significant internal pore diffusion limitation was found since the value of effectiveness factor was 0.95. The operational half life in a column reactor at pH 8.0 was 6.8 days at 4$0^{\circ}C$ and 47 days at 3$0^{\circ}C$, whereas that of without entrappment was only 1 day and 4 days, respectively. The performance of a batch and a column reactor was also discussed with respect to the productivity. The results demonstrated that the entrappment of an adsorbed enzyme for the enhancement of the operational stability of the immobilized enzyme was useful especially when an extracellular enzyme was used.

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Flight Test of Helicopter Landing System Using Real-time DGPS (실시간 DGPS를 이용한 헬리콥터 착륙 시스템 개발)

  • Park, Sung-Min;Kim, Jung-Han;Whang, Duk-Ho;Jang, Jae-Gyu;Kee, Chang-Don;Park, Hyoung-Taek;Park, Hong-Man;Lee, Chang-Hyo
    • Journal of Advanced Navigation Technology
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    • v.3 no.2
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    • pp.108-119
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    • 1999
  • In recent, there has been remarkable progress in the field of GPS applications. In a few years, an appreciable number of aircraft will adopt GPS as a landing guidance system because GPS is more economic, more reliable and more accurate than any other aviation systems. In this respect, we have performed several helicopter landing flight tests based on the real-time DGPS system made in SNUGL (Seoul National University GPS Laboratory). From the experimental results, we found several problems Which should be fixed to adopt DGPS as a aircraft landing guidance system. In this paper, we will introduce the problems found in tests and also suggest modifications to solve the problems. Our modifications can be classified into three parts. The first is about the attitude determination with single GPS antenna. The second deals with the cockpit display module. The display was devised to integrate the Instrument Landing System(ILS) with tunnel-the-sky using virtual reality. With the display, pilot can achieve more safe landings. The last part is the digital map. We inserted digital map into our system and put direction indicator on the map using position information from GPS. It is very useful for pilot to find airports even in bad weather. Using the newly designed DGPS landing system, we conducted flight test at Kimhae International Airport, Pusan, Korea. It was successful! Our system can also satisfy Category-I criterion for aircraft landing approach and determine attitude angle with a high level of reliability. It is supported by video materials.

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Energy Perspective of Sugar Industries in Pakistan: Determinants and Paradigm Shift

  • Siddiqui, Muhammad Ayub;Shoaib, Adnan
    • Journal of Distribution Science
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    • v.10 no.2
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    • pp.7-17
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    • 2012
  • The aim of this study is to empirically explore micro and macroeconomic factors affecting the Pakistani sugar industries and searching the energy potential of this industry, through the survey of literature. The empirical part has been explored by employing Vector Autoregression (VAR), Granger Causality tests and simultaneous equation models through quarterly data for the period of 1991q2-2008q4. The study also aims to devise policies for the development of sugar industries and identify its growing importance for the energy sector of Pakistan. Empirical tests applied on the domestic prices of sugar, domestic interest rates, and exchange rate, productive capacities of sugar mills, per capita income, world sugar prices on cultivable area and sugar production reveal very useful results. Results reveal an improvement of productive capacity of the sugar mills of Pakistan on account of increasing crushing capacity of this sector. Negative effect of rising wholesale prices on the harvesting area was also observed. Profit earnings of the sugar mills significantly increase with the rise of sugar prices but the system does not exist for the farming community to share the rising prices of sugar. The models indicate positive and significant effect of local prices of sugar on its volume of import. Another of the findings of this study positively relates the local sugar markets with the international prices of sugar. Additionally, the causality tests results reveal exchange rate, harvesting area and overall output of sugarcane to have significant effects on the local prices of sugar. Similarly, import of sugar, interest rate, per capita consumption of sugar, per capita national income and the international prices of sugar also significantly affect currency exchange rate of Pakistani rupee in terms of US$. The study also finds sugar as an essential and basic necessity of the Pakistani consumers. That is why there are no significant income and price effects on the per capita consumption of sugar in Pakistan. All the empirical methods reiterate the relationship of variables. Economic policy makers are recommended to improve governance and management in the production, stock taking, internal and external trading and distribution of sugar in Pakistan using bumper crop policies. Macroeconomic variables such as interest rate, exchange rate per capita income and consumption are closely connected with the production and distribution of sugar in Pakistan. The cartelized role of the sugar industries should also be examined by further studies. There is need to further explore sugar sector of Pakistan with the perspective of energy generation through this sector; cartelized sugar markets in Pakistan and many more other dimensions of this sector. Exact appraisal of sugar industries for energy generation can be done appropriately by the experts from applied sciences.

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The Effect of C Language Output Method to the Performance of CGI Gateway in the UNIX Systems (유닉스 시스템에서 C 언어 출력 방법이 CGI 게이트웨이 성능에 미치는 영향)

  • Lee Hyung-Bong;Jeong Yeon-Chul;Kweon Ki-Hyeon
    • The KIPS Transactions:PartC
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    • v.12C no.1 s.97
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    • pp.147-156
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    • 2005
  • CGI is a standard interface rule between web server and gateway devised for the gateway's standard output to replace a static web document in UNIX environment. So, it is common to use standard I/O statements provided by the programming language for the CGI gateway. But the standard I/O mechanism is one of buffer strategies that are designed transparently to operating system and optimized for generic cases. This means that it nay be useful to apply another optimization to the standard I/O environment in CGI gateway. In this paper, we introduced standard output method and file output method as the two output optimization areas for CGI gateways written in C language in the UNIX/LINUX systems, and applied the proposed methods of each area to Debian LINUX, IBM AIX, SUN Solaris, Digital UNIX respectively. Then we analyzed the effect of them focused on execution time. The results were different from operating system to operating system. Compared to normal situation, the best case of standard output area showed about $10{\%}$ improvement and the worst case showed $60{\%}$ degradation in file output area where some performance improvements were expected.