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Immunohistochemical Detection of N-myc Gene Product by Using Antiserum Against Synthetic Peptide (항-펩타이드 항체를 이용한 암유전자 N-myc 산물의 면역조직화학적 검출)

  • Lee, Hyun-Chul;Lee, Wan-Joo;Ahn, Tai-Hew
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.2
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    • pp.167-174
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    • 1987
  • N-myc, a DNA sequence related to the oncogene c-myc, was found to be amplified in untreated primary neuroblastomas and the amplification appeared to be associated with advanced disease at diagnosis and rapid tumor progression. Synthetic peptides have been useful immunogens for generating antisera and monoclonal antibodies to a number of native proteins. In order to identify myc-related protein in the tumor cells, an antiserum against a synthetic hexapeptide (-Glu-Asp-Ile-Trp-Lys-Lys-), whose sequence corresponds to a part of the exon 2 of oncogene N-myc, was prepared by immunizing a rabbit with BSA-conjugated peptide. After ammonium sulfate precipitation and affinity column chromatography, it appeared to be specific to the peptide. Strong nuclear staining in immunoperoxidase method using this serum was observed in both human promyeloid leukemic cell line, HL-60(containing high c-myc copy number), and human neuroblastoma cell line, LA-N-5 (containing high N-myc copy number), whereas LA351 (human lymphoid cell line) cells did not react with the serum. This reaction was completely abrogated by incubating the antiserum with soluble excess peptide. These data suggest that the protein encoded by N-myc could be localized in the nucleus as c-myc protein and this antiserum can be used to detect myc-related tumor cells in clinical samples and to determine if the N-myc expression correlates with genomic amplification in cell lines, untreated primary tumors, and untreated metastases.

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Comparison of Soil Physical and Chemical Properties between Coniferous and Deciduous forests in Mt. Palgong (침.활엽수림에서 산림토양의 이.화학적 특성 비교)

  • Hur, Tae-Chul;Joo, Sung-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.39-47
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    • 2002
  • This study was carried out in order to produce useful material for the forest multiple use and forest protection by soil physico-chemical analysis of studied area in Mt. Palgong. The results of soil physico-chemical analysis and statistical analysis represented as following 2 points. 1. Soil depth was in the range of average 61.1 cm and soil texture was loamy sand and sandy loam except Donghwasa area. The part of solid phase and gaseous phase were higher than other areas, but liquid phase was less in verse. Soil water content was in an average 49.5%, penetrability was average $1.95{\times}E^{-2}cm/sec$ and the average of soil hardness was $1.64Kg/cm^2$. This data showed that soil water content, penetrability and soil hardness were good at Mt. Palgong forest soils. 2. Soil pH was the range of 3.4 to 6.0, organic carbon content was 2.8% that is nearly mean of the Korea brown forest soils, total N content is somewhat smaller than that of other places, and total average C/N ratios was 13.9. Average available $P_2O_5$ concentration was 5.05 mg/kg that is lower than that of any others. The concentration of available P of coniferous forests is higher than that of deciduous forests. Exchangeable cations content is similar to those of the Korea brown forest soil and the order of the cation content extent is $Ca^{2+}$ > $Mg^{2+}$ > $Na^+$ > $K^+$.

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Gender Wage Differentials in the Arts and Cultural Sector (문화예술분야에서 성별임금격차 분석연구)

  • Heo, Shik;Sa, Myung-Cheol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.11
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    • pp.4151-4160
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    • 2010
  • Recently the arts and cultural sector is drawing attention as a new growth engine of the Korean economy. The paper is empirically analyzed on the gender wage differentials in the arts and cultural sector and specially focused on decomposing the wage differentials between the manufacturing sector and the arts and cultural sector. Our results may be partly useful for explaining the existing phenomenon of the arts and cultural sector in Korean labor markets, for example, young, highly educated, part-time contracts, less gender discrimination, etc. The results might provide somehow the policy directions of reducing the gender wage differentials.

Analysis of Furnace Conditions with Waste Plastics Injection into Blast Furnace (폐플라스틱의 吹入에 따른 高爐 爐況解析)

  • 허남환;백찬영;임창희
    • Resources Recycling
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    • v.9 no.6
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    • pp.23-30
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    • 2000
  • Since most of the waste plastics are incinerated and landfilled for the plastic treatment, the environmental friendly processes must be introduced. The plastic utilization of plastic to the blast furnace as a substitutional fuel was developed as a useful recycling method of waste plastics, and commercialized in several ironmaking company in Europe and Japan. Present study was carried out to understand the effect of plastic injection on blast furnace process continuously by using the foundry blast furnace in POSCO. The coke replacement ratio turned out to be 0.98 with the waste plastic injection up to 13.8 kg/thm of injection rate, and there were no significant effect of the kinds of injection plastics on the replacement ratio in this test operation. The permeability in the furnace became worse and the heat load in the lower part of blast furnace was increased with increasing the injection rate of waste plastics. As the rate of plastic injection were increased, the top gas utilization and shaft efficiency were also decreased from the Rist diagram analysis.

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A Markup Language for Describing the Linkage between Sensor Data and Service in the Ubiquitous Environment (유비쿼터스 환경에서 센서 데이터와 서비스의 연계를 표현하는 마크업 언어)

  • Lee, Hun-Soon;Jin, Seung-Il
    • The KIPS Transactions:PartD
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    • v.15D no.2
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    • pp.247-256
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    • 2008
  • In the ubiquitous environment, it is scattered all over our neighboring in many smart objects. These smart objects constantly produce the information and the amount of the generated information is massive. As the internet search engine came out to help us to find the useful data from the sea of the information connected to the internet, the sensor data stream processing middleware is appearing to make us to develop the ubiquitous service easily by extracting the meaningful information from the massive sensor data and delivering the extracted information to the application which makes our life convenient. We have to inform the information relating to the provided service to a middleware so that the ubiquitous service can be provided by using sensor data stream processing middleware. In this paper, we classify the information which is needed to express the ubiquitous service which uses sensor data for the service providing. And we propose a distinct markup language called Context-driven Service Markup Language (CSML) to effectively describe this information. We can easily express the various ubiquitous services which have to be provided in the various situations using proposed CSML.

A Case Study on Selection and Improvement of SLA Evaluation Metrics (SLA 평가 지표 선정과 개선 방안에 관한 사례 연구)

  • Shin, Sung-Jin;Rhew, Sung-Yul;Kim, Yoo-Ri
    • The KIPS Transactions:PartD
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    • v.16D no.4
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    • pp.541-548
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    • 2009
  • Many companies have recently apply SLA and execute IT service by using SLA. However, there are no objective standards for selection and improvement of SLA evaluation metrics. We derive and present measurement attributes that are criteria for selection and improvement of SLA evaluation metrics as measurement metrics. We execute a case study based on D company in order to verify whether the measurement metrics are applicable. We apply and evaluate the measurement metrics that are applicable to D company, and then we designate an improvement line. We propose improvement guidelines of the measurement metrics which score is less than the improvement line's and derive SLA evaluation metrics. We prove that the way of selection and improvement is useful by applying SLA evaluation metrics to D company.

The Regional Folk Plants in Ulleung Island, Korea (울릉도지역의 민속식물)

  • Jeong, Hye-Ran;Choi, Kyung;Moon, Hyun-Shik
    • Journal of agriculture & life science
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    • v.46 no.5
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    • pp.25-35
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    • 2012
  • This study was carried out to classify the folk plants in Ulleung island. According to the survey results from 553 sheets of 55 residents at 20 places from march 2009 to september 2010, the folk plants in Ulleung island consisted of a total 113 taxa; 51 families, 93 genera, 98 species, 2 subspecies and 13 varieties. Among the investigated 113 taxa; 5 Korean endemic plants, 14 rare plants and 5 naturalized plants were also included. The use by usage of 113 taxa was; 72 as medicinal, 65 as edible, 5 as ornamental, etc., respectively, so the medicinal use is the highest. The most useful part was leaf, followed by fruit and root. The consistency comparison between the korean name and the local name were the highest in the people in 40's and the lowest in those in 70's.

Clay mineral distribution and provenance in surface sediments of Central Yellow Sea Mud

  • Koo, HyoJin;Lee, YunJi;Kim, SoonOh;Cho, HyenGoo
    • Geosciences Journal
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    • v.22 no.6
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    • pp.989-1000
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    • 2018
  • The provenance of the Central Yellow Sea Mud (CYSM) in the Yellow Sea has been attracted a great deal of attention over the last three decades, but a consensus is not yet reached. In this study, 101 surface sediment samples collected from the CYSM were investigated to determine provenance and transport mechanisms in the area using the clay minerals and major element components. The Huanghe sediments are characterized by higher smectite, but the Changjiang sediments are more abundant illite contents. Western Korean rivers contain more kaolinite and chlorite than do Chinese rivers. The Chinese rivers have higher $Fe_2O_3$, MgO, and CaO than the Korean rivers at the same $Al_2O_3$ concentration. Therefore, the clay minerals and major element concentrations can be useful indicator for the source. Based on our results, we suggest that the surface sediments in CYSM were composed mainly of Changjiang sediments, mixed a partly with sediments from the Huanghe and the western Korean rivers. Although the northwestern part of CYSM is proximate to the Huanghe, the contents of smectite and CaO were extremely low. It could be evidence that the Huanghe materials do not enter directly into the CYSM due to the Shandong Peninsula Front. Considering the oceanic circulation in the Yellow Sea, the Changjiang sediments could be transported eastward with the Changjiang Diluted Water and then mixed in CYSM via the Yellow Sea Warm Current (YSWC). Huanghe sediments could be provided by coastal currents (Shandong Coastal Current and Yellow Sea Coastal Current) and the YSWC. In addition, sediments from western Korean rivers might be supplied into the CYSM deposit via the Korean Coastal Current, Transversal Current, and YSWC.

Nondestructive Bending Strength Evaluation of Woodceramics Made from Woody Part of Broussonetia kazinoki Sieb. -Effect of Carbonization Temperature- (닥나무의 목질부로 만든 우드세라믹의 비파괴휨강도평가 -소성온도의 영향-)

  • Byeon, Hee-Seop;Won, Kyung-Rok;Lee, Ho-Young;Oh, Seung-Won
    • Journal of agriculture & life science
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    • v.46 no.1
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    • pp.35-41
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    • 2012
  • Nondestructive evaluation (NDE) technique method using a resonance frequency mode was carried out for woodceramics made by different carbonizing temperature (600, 800, 1000, $1200^{\circ}C$) for Broussonetia kazinoki Sieb. Dynamic modulus of elasticity increased with increasing carbonizing temperature. There was a close relationship of dynamic modulus of elasticity and static bending modulus of elasticity to MOR. Therefore, the dynamic modulus of elasticity using resonance frequency mode is useful as a nondestructive evaluation method for predicting the MOR of woodceramics made by different carbonizing temperature for B. kazinoki Sieb.

Geological Application of Lineaments from Satellite Images - A Case Study of Euiseong Sub-basin (위성 영상선구조의 지질학적 응용 - 의성소분지의 경우)

  • 김원균;김상완;원중선;민경덕;김정우
    • Korean Journal of Remote Sensing
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    • v.16 no.1
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    • pp.25-36
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    • 2000
  • To evaluate the feasibility of using lineaments for the interpretation of regional geological structures, the extracted lineaments from satellite image and surveyed surface geological features mapped in the field were analyzed for the Euiseong Sub-basin. The lineaments extracted from Landsat-5 TM images show primary directions of N20$^{\circ}$~30$^{\circ}$E, N60$^{\circ}$~70$^{\circ}$E, N60$^{\circ}$~70$^{\circ}$W, which represent the trends of faults, strikes, and joints. In the sedimentary formation in the northern part of Palgongsan Uplift Zone, primary directions of the lineaments are NNE and NWW, and NEE in southern parts. The analysis of satellite lineaments is proved to be very useful to study the large-scale structures and surface geology of the Euiseong Sub-basin, whereas the previous research using brittle tectonics approach was advantaged in the outcrop scale in interpretation.