• Parasites, Hosts and Diseases
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• v.55 no.3
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• pp.233-238
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• 2017

Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp., a defined, slightly modified liquid media is used. In this study, in order to generate pure N. fowleri cysts, a liquid encystment medium (buffer 1) modified from Page's amoeba saline was applied for encystation of N. fowleri. N. fowleri cysts were well induced after 24 hr with the above defined liquid encystment medium (buffer 1). This was confirmed by observation of a high expression of differential mRNA of nfa1 and actin genes in trophozoites. Thus, this liquid medium can replace the earlier non-nutrient agar medium for obtaining pure N. fowleri cysts. In addition, for cyst formation of Acanthamoeba spp., buffer 2 (adjusted to pH 9.0) was the more efficient medium. To summarize, these liquid encystment media may be useful for further studies which require axenic and pure amoebic cysts.

• Korean Journal of Plant Resources
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• v.30 no.6
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• pp.699-706
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• 2017

For rapid production of freesia 'Shiny Gold' shoots by using a bioreactor, several culture conditions were investigated. Young shoots (< 1 cm) obtained from freesia corm section in vitro were used as plant materials for this experiment. As a basic experimental environment, 20 young shoots were inoculated into a 5 L balloon type bubble reactor which contained 1 L 1/2 strength MS medium supplemented with 30 g sucrose (3%), and the aeration was 0.1 vvm (vessel volumes per minute). The bioreactors were placed in a growth room with $23^{\circ}C$ temperature, 60% relative humidity and $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light condition (16 h/8 h, day/night). The concentrations of MS media were set with 1/4, 1/2, 1 strength, medium volume 10, 20, 40%, sucrose concentration 3, 6, 9%, and aeration 0.1, 0.2, 0.4 vvm. After 4 weeks of cultivation, the growth indexes including the fresh and dry weight, and plant height were evaluated. At the same time, the consumption, pH, and EC of medium were estimated 4 weeks after incubating. The best results were achieved when 40 young shoots were incubated in a bioreactor in which 1 L of 1/2 strength MS medium supplemented with 6% sucrose was used for the rapid production of freesia shoots. The shoots were 17 cm in plant height and 1.0 g in fresh weight only 4 weeks after incubation which could be a good plant material suitable for corm enlargement in vitro. No correlation was observed between the growth of freesia shoots and the consumption, pH or EC of medium.

• Nuclear Engineering and Technology
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• v.56 no.5
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• pp.1747-1753
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• 2024

The accurate determination of formation density and the physical properties of rocks is the most critical logging tasks which can be obtained using gamma-ray transport and detection tools. Though the simulation works published so far have considerably improved the knowledge of the parameters that govern the responses of the detectors in these tools, recent studies have found considerable differences between the results of using a conventional model of a homogeneous mixture of formation and fluid and an inhomogeneous fractured medium. It has increased concerns about the importance of the complexity of the model used for the medium in simulation works. In the present study, we have suggested two various models for the flow of the fluid in porous media and fractured rock to be used for logging purposes. For a typical gamma-gamma logging tool containing a ¹³⁷Cs source and two NaI detectors, simulated by using the MCNPX code, a simplified porous (SP) model in which the formation is filled with elongated rectangular cubes loaded with either mineral material or oil was investigated. In this model, the oil directly reaches the top of the medium and the connection between the pores is not guaranteed. In the other model, the medium is a large 3-D matrix of 1 cm³ randomly filled cubes. The designed algorithm to fill the matrix sites is so that this realistic random (RR) model provides the continuum growth of oil flow in various disordered directions and, therefore, fulfills the concerns about modeling the rock textures consist of extremely complex pore structures. For an arbitrary set of oil concentrations and various formation materials, the response of the detectors in the logging tool has been considered as a criterion to assess the effect of modeling for the distribution of pores in the formation on simulation studies. The results show that defining a RR model for describing heterogeneities of a porous medium does not effectively improve the prediction of the responses of logging tools. Taking into account the computational cost of the particle transport in the complex geometries in the Monte Carlo method, the SP model can be satisfactory for gamma-gamma logging purposes.

• Journal of Korean Society of Forest Science
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• v.80 no.4
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• pp.436-444
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• 1991

To increase both the production of mushrooms and the usages of the residues (used media) for roughage, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The contents of crude proteins(1.82-4.55%) in the used media were higher than those in woods (c.a. 0.7%), and the total digestible nutrients (44.0-46.0%) in the used medium were not much different from those in rice straws (c.a.48.0%). 4. The contents of some essential amino acids (methionine in the used medium of the tannic acid added PC or S.D. and isoleucine, phenylalanine and lysine in the used medium of the untreated PC or SD) were higher than those in the control (raw media). The contents of a few other essential amino acids (threonine, valine and arginine) were lower in the used media. Most nonessential amino acids in the used media, particularly in the used bed-log, appeared to decrease than those in the control.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.4
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• pp.692-697
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• 2016

This study examined the effects of salinity and medium used on the induction of sexual reproduction for the mass production of the resting eggs in the marine rotifer Brachionus rotundiformis for 9 days. After exposure to various salinities, the mixis rate in all treatments began to increase on the third day and decreased after the 4th or 5th day. The resting eggs appeared on the 4th day at 10‰, 15‰, 20‰, 25‰. In particular, the fertilization rates with 33.3% and 31.3% at 20‰ and 25‰ on day 6 were higher than those of the others, respectively. Mean mixis rate for 9 day at 0‰ and 20‰ were the highest with 25.3% and 20.2%, respectively, and the fertilization rate (14.0%) at 25‰ was the highest. In the medium experiment used, the mixis rate in all treatments were similar to the results of the salinity experiment. The resting eggs were only found at 0% and 25% of the used medium treatments. While there were no significant differences in the mean fertilization rate of all treatments, the mean mixis rate (22.3%) in the 25% treatment was higher than that of the control (0% treatment). As a result, salt stress was more effective than using the used medium to induce sexual reproduction in B. rotundiformis. The optimal salinity was considered to be 20-25‰, which caused a high mixis and fertilization rate in the rotifer. These results provide basic data for the mass production of rotifer resting eggs.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.15 no.2
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• pp.1-8
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• 2007

In this research, algorithm and software for the medium frequency signal analysis of DGPS(Differential Global Positioning System) station were developed. Based on new MF(Medium Frequency) algorithm, the software of NDGPS(National DGPS) signal analysis was developed for coverage analysis. Predicted MF propagation data from this software was compared to the measurement data for the verification of a developed MF algorithm. GIS(Geographic Information System) techniques including digital map with elevation data were used because MF propagation is closely related to ground conductivity, mountains, building intensity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.18 no.36
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• pp.193-203
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• 1995

The production management system software which can be applied In the medium and small companies is developed. Since the medium and small companies hesitate to install the computer system for production management owing to the lack of funds and the complexities of operation, the system is made to be used in PC and only requires minimum input data. This system is composed of interrelated modules for receive/order release, inventory management MRP, new product registration, document management and production scheduling. In this paper the architecture of the system, functions of each module, and information processing procedures of each funtion are discussed.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2007.04a
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• pp.317-321
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• 2007

"The project for production of medium classification landcover maps using satellite images" has been completed from 1998 until 2005 in Korea. As the 5th project was finished in 2005, medium classification landcover maps for all areas of South Korea have been produced. The products of project currently is used in lots of fields such as public governments, universities and research institutes for policy application and scientific research. But final results of the project have several problems which is insufficiency of reliability, discordance of classification codes and many others because each project was progressed year by year. In this study, problems of existing production methods about medium classification landcover maps are extracted and solution of problems is offered. Therefore, this study will make it possible to efficiently produce and update medium classification landcover maps.

• Coupled systems mechanics
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• v.1 no.4
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• pp.297-309
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• 2012

As a result of the medium coupling, propagation characteristics of ultrasonic waves guided by a multi-phase medium can be different from those in a homogeneous system. This phenomenon becomes prominent for a medium consisting of phases with considerably distinct material and physical properties (e.g., submerged structures or human bones covered with soft tissues). In this study, the coupling effect arising from both fluid and soft tissues on wave propagation in engineering structures and human bone phantoms, respectively, was explored and calibrated quantitatively, with a purpose of enhancing the precision of ultrasonic-wave-based non-destructive evaluation (NDE) and clinical quantitative ultrasound (QUS). Calibration results were used to rectify conventional NDE during evaluation of corrosion in a submerged aluminium plate, and QUS during prediction of simulated healing status of a mimicked bone fracture. The results demonstrated that with the coupling effect being appropriately taken into account, the precision of NDE and QUS could be improved.

• Asian Journal of Turfgrass Science
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• v.12 no.4
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• pp.195-202
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• 1998

The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory.