• Title/Summary/Keyword: Used medium

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Improvement of Regeneration Efficiency from Mature Embryo and Leaf Base Segment in Korean Oat Genotypes

  • Kim Kyung-Hee;Moon Jung-Hun;Lee Sang-Kyu;Lee Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.349-353
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    • 2004
  • Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4­D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at $25^{\circ}C$ in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin $(91.8\%)$. In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.

Investigation of Selective Medium for Isolation and Enumeration of Bacteroides sp. from the Feces of the Korean People (한국인의 분변으로 부터 Bacteroides를 분리하기 위한 선택 배지 조사)

  • Ji, Geun-Eog;Kim, In-Hee;Lee, Se-Kyung
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.295-299
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    • 1994
  • Vancomycin inhibited the growth of the most large intestinal bacteria other than Bacteroides sp. and E. coli. Since Bacteroides are predominant compared with E. coli in the large intestinal tract of the adult, vancomycin-added medium(VA) was shown to be an effective selective medium for Bacteroides. VA showed comparable or better selectivity for Bacteroides sp. to NBGT which is being used currently as a most frequently used selective medium for Bacteroides in Japan.

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EVALUATION 01 OIL DISPERSION AGENT BY ASSESSMENT 01 COLOR STRENGTH 01 ORGANIC PIGMENT

  • H., Young-Chan;R., Seo-Joon;L., Dong-Wook;H., Soon-Taek
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.24 no.3
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    • pp.73-80
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    • 1998
  • This Study was performed to get the suitable oil dispersion agent by assessment of color strength of organic pigment in non-aqueous systems. Organic pigment is used as a color expression material with other body pigments in the make-up products. But occasionally aggregation or agglomeration occurs for the lack of affinity with medium, This function is the cause of disturbing homogeneous dispersion, and then bring about an instability of products. Our study, research of dispersion mechanism between the pigment and oil phase, has been executed to solve this problem, and find a oil dispersion agent having optimum dispersion condition. Generally dispersion is related to between the solid-liquid mutual properties and electrical phenomena associated with solid-liquid interface. This factor is determined to input energy, milling time, optical properties, particle size, rheological properties, etc. Ideal dispersion state is told that coloring primary solid particle is homogeneously dispersed in medium. Good dispersed colorants are strongly and clearly appeared. We are already known that the particle size of organic pigment, chemical properties and viscosity of medium, refractive index. Consequently We determine the affinity of medium and organic pigment by measuring of color strength in the same mechanical condition. UV-VISIBLE RECORDING SPECTRO PHOTOMETER is used for measuring apparatus. We can decided the dispersion level of oil dispersion agent by measuring absorbance of color strength in the visible range that diluted medium for colloid colorant particles.

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A novel two-dimensional approach to modelling functionally graded beams resting on a soil medium

  • Chegenizadeh, Amin;Ghadimi, Behzad;Nikraz, Hamid;Simsek, Mesut
    • Structural Engineering and Mechanics
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    • v.51 no.5
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    • pp.727-741
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    • 2014
  • The functionally graded beam (FGB) is investigated in this study on both dynamic and static loading in case of resting on a soil medium rather than on the usual Winkler-Pasternak elastic foundation. The powerful ABAQUS software was used to model the problem applying finite element method. In the present study, two different soil models are taken into account. In the first model, the soil is assumed to be an elastic plane stress medium. In the second soil model, the Drucker-Prager yield criterion, which is one of the most well-known elastic-perfectly plastic constitutive models, is used for modelling the soil medium. The results are shown to evaluate the effects of the different soil models, stiffness values of the elastic soil medium on the normal and shear stress and free vibration properties. A comparison was made to those from the existing literature. Numerical results show that considering real soil as a continuum space affects the results of the bending and the modal properties significantly.

Diagnosis of Medium Voltage Cables for Nuclear Power Plant

  • Ha, Che-Wung;Lee, Do Hwan
    • Journal of Electrical Engineering and Technology
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    • v.9 no.4
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    • pp.1369-1374
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    • 2014
  • Most accidents of medium-voltage cables installed in nuclear power plants result from the initial defect of internal insulators or the initial failure due to poor construction. However, as the service years of plants increase, the possibility of cable accidents is also rapidly increases. This is primarily caused by electric, mechanical, thermal, and radiation stresses. Recently, much attention is paid to the study of cable diagnoses. To date, partial discharge and Tan${\delta}$ measurements are known as reliable methods to diagnose the aging of medium-voltage cables. High frequency partial discharge measurement techniques have been widely used to diagnose cables in transmission and distribution systems. However, the on-line high frequency partial discharge technique has not been used in the nuclear power plants because of the plant shutdown risk, degraded measurement sensitivity, and application problems. In this paper, the partial discharge measurement with a portable device was tried to evaluate the integrity of the 4.16kV and 13.8kV cable lines. The test results show that the high detection sensitivity can be achieved by the high frequency partial discharge technique. The present technique is highly attractive to diagnose medium voltage cables in nuclear power plants.

A New Selective Medium for Detecting Acidovorax avenae subsp. avenae in Rice Seeds

  • Song, Wan-Yeob;Kang, Mi-Hyung;Kim, Hyung-Moo
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.236-241
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    • 2000
  • A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.

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The Effects of MPPA (Mileages/Points to Purchase Amount) Ratio on Consumers' Preference (구매금액 대비 마일리지/포인트의 비율이 소비자 선호에 미치는 영향)

  • Park, Sang-June;Byun, Ji-Yeon
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2008.10a
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    • pp.179-190
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    • 2008
  • Consumers earn a number of points for every purchase and then they can exchange a specified number of points for a desired reward in a typical loyalty program. The immediate payoff of their effort given as points is not the real reward they actually care about. It is merely an instrument (or medium) which has no value in itself. In a real world, consumers frequently choose the option with a bigger medium even though the economic value of the option is not changed by the medium. We call it 'medium effect'. In this study we explored if the size of medium affects on consumers' preferences. For this we controlled the reward options with three types of medium (small, medium, big) and measured the magnitude of preference difference among the three types of reward options. In addition, we manipulated comparability of reward options with wine and gas discount coupon. We confirmed that choosing one of two wines was easier than that of the two gas coupons. 164 respondents were allocated into three experimental groups and one control group. In three experimental groups, the ratios of the focused reward option's medium to the compared reward option's medium were different. For example, the focused reward option has 10 million points whereas the compared reward option has 10 million points for 1 million won purchase amount in the first group. Then each respondent was asked to choose one of two loyalty programs (focused program vs. compared program) in two different conditions (comparability between reward options: easiness vs. difficulty). To compare the medium effects among the experiment conditions we used chi-squares tests. The empirical results show the medium effect increases and then decreases as the ratio of reward mileages/points to purchase amount increases. Additionally, they let us know that comparability of alternatives affects on the medium effects depending on the ratio of reward mileages/points to purchase amount.

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Transcervical Embryo Recovery in Korean Black Goats: A Preliminary Experiment

  • Lee, Doo-Soo;Kim, Dong-Woo;Shin, Sang Tae
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.171-174
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    • 2015
  • Four pluriparous Korean black goat does were superovulated with FSH and mated with fertile bucks. Anesthetized animals were placed in lateral recumbency, then size 8 Foley catheter was inserted into the uterus through the cervix under the vaginal speculum and the balloon was inflated to fix the catheter in the uterine body. The opposite end of the catheter was connected to a 3-way and a flushing medium was infused into the uterus. Modified Dubecco's PBS with 1% FBS was used as the flushing medium. Four goats were allocated in two groups depending on the type of medium infusion into uterus. Injection group; the flushing medium was injected into uterus and the infused medium was collected by to-and-fro method using a syringe. Gravity-flow group; the flushing medium was allowed to enter the uterus by gravity flow by lifting the medium bottle and drained out of the uterus into a collecting tube. All four goats had catheter inserted through the cervix and uteri flushed successfully. The volume (recovery rate) of recovered medium varied considerably from 87 ml/200 ml (43.5%) to 148 ml/160 ml (92.5%). Nine embryos/ova in total were recovered from Gravity-flow group goats. Although the embryo recovery rate was low, the possibility of a transcervical embryo recovery in Korean black goat had been proven in this preliminary experiment.

1H-NMR Analysis according to the Storage Period after the Use of Iopamidol and Ioversol Formulations Contrast Agents (Iopamidol과 Ioversol 제제 조영제의 사용 후 보관 기간에 따른 1H-NMR 분석)

  • Han, Beom-Hee
    • Journal of radiological science and technology
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    • v.44 no.3
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    • pp.239-245
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    • 2021
  • Since the side effects of contrast agents occur in many ways, hospitals must clearly identify the contrast agents and know how to manage them. Storage of contrast media It is common practice to store contrast media in a warm room to reduce viscosity and improve patient comfort. However, it is important to recognize that long-term storage at high temperatures can shorten the shelf life of a product. Therefore, in this study, the presence or absence of chemical changes according to the storage period after using the contrast medium was analyzed. As the analysis equipment, a 500 MHz Nuclear Magnetic Resonance Spectrometer of Bruker Avance (Germany) possessed by Korea Basic Science Institute (KOREA BASIC SCIENCE INSTITUTE; KBSI) was used. For the X-ray iodide contrast medium, Pamiray contrast medium of Iopamidol and Optiray contrast medium of Ioversol, samples remaining after 7 days, 20 days, 30 days, and 1 year were collected, By acquiring the spectrum from the 1H-NMR spectrometer of the contrast medium stimulated by It was compared and analyzed with the standard sample. In conclusion, depending on the storage period of the contrast medium, no peaks of physical and chemical changes were observed in both the Pamiray contrast medium of Iopamidol and the Optiray contrast medium of Ioversol after 7 days, 20 days, 30 days, and 1 year after use.

Effect of Substrates on the Growth and Flowering of Freesia hybrid 'Gold Rich' in Nutrient Culture (프리지아(Freesia hybrida) '골드리치'의 양액재배 시 인공배지별 생육 및 개화특성)

  • Lee, Jin-Jae
    • Horticultural Science & Technology
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    • v.35 no.1
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    • pp.30-37
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    • 2017
  • This study was conducted to determine the effects of various growth substrates on the growth and flowering of hydroponically grown Freesia hybrid 'Gold Rich'. Perlite, peat moss and a perlite: peat moss mixture (1 : 1 ratio, v / v) were used as the growing media. The greatest plant height before flower bud differentiation was attained using mixed medium compared to the others. The type of medium used did not influence leaf number, mineral content or SPAD value in leaves. Flowering began at 137 days after planting in mixed medium, which was 13 days earlier than in perlite medium. The whole plant fresh weight was 21.3 g heavier in mixed medium than in perlite medium (40.9 g). A similar result was obtained for shoot length, with the highest value (96.6 cm) obtained in mixed medium, i.e., 20 cm higher than in perlite medium (76.6 cm). Floret number per plant was also the highest in mixed medium (14.4), i.e., 1.7 - times higher than in perlite medium. Therefore, among the substrates tested in this experiment, we recommend using mixed perlite: peat moss medium (1 : 1 ratio, v / v) for hydroponic culture of freesia, as the use of this medium improved the physical properties of the plants, producing the best results in terms of plant growth and cut-flower quality.