• 대한미생물학회지
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• 제22권3호
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• pp.301-307
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• 1987

A total of 42 strains of Candida albicans were examined for susceptibility to three antifungal agents, amphotericin B(AMB), 5-fluorocytosine(5-FC), and ketoconazole(KTZ), using defined medium, synthetic amino acid medium-fungal(SAAM-F), supplemented yeast nitrogen base(SYNB) and undefined medium Sabouraud's dextrose broth(SDB) and Kimmig broth media. A tube dilution method was used with minimum inhibitory concentrations(MICs) determined after incubation for 24 hour and 48 hours. All testes were performed in duplicate. In general, MICs were more reproducible after 48 hour of incubation. Forthermore, MICs determined after incubation for 48 hours were significantly higher than those determined after 24 hours. The actural MICs obtained with the different antifungal agents were clearly influenced by the test medium used. The rank order of AMB MICs according to the test medium was as follows: SAAM-F>SYNB>SDB>Kimmig broth. With 5-FC, the following pattern was observed: SYNB>SAAM-F>SDB>Kimmig borth. For ketoconazole, the MICs according to the test medium was SAAM-F>SDB>SYNB> Kimmig broth. In amphotericin B, the MICs mean value with the test medium was as follows: SDB, 0.24 mcg/ml; Kimmig broth, 0.29 mcg/ml; SYNB, 0.21 mcg/ml and SAAM-F, 0.15mcg/ml. The actural value of 5-FC was; SDB, 37.20 mcg/ml; Kimmig broth, 67.41mcg/ml; SYNB, 21.29 mcg/ml and SAAM-F, 24.61 mcg/ml and in ketoconazole, the MICs value was; SDB, 1.83 mcg/ml; Kimmig broth, 4.08 mcg/ml; SYNB, 1.95 mcg/ml and SAAM-F, 1.41 mcg/ml. The results of this investigation suggested that broth dilution susceptibility testing of yeast and yeast-like fungi are best performed with an incubation period of 48 hours. Furthermore, medium composition can significantly influence the results of such testing.

• 환경영향평가
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• 제21권5호
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• pp.609-615
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• 2012

In recent years, the development of computer technique was possible to the simulation analysis of the structure caused by ground vibration. Generally, finite element method(FEM) has been used in these structural analysis. In this study, it was calculated to the vibration energy as measuring vibration waveform, and estimated about principal stress due to medium characteristics of the ground as processing dynamic analysis by the vibration energy. The results are as follows : Firstly, the principal stress distribution in all mediums was different due to a medium condition, and the principal stress at concrete medium was represented to difference due to physical characteristics. Secondly, the principal stress by time increasing was represented to maximum amplitude within 0.03 second. And also, the principal stress after maximum amplitude was very large at concrete medium, which was considered to be formed compression or tension range at a medium boundary. Thirdly, the variation of principal stress at concrete medium was represented in the order of RC medium, NC=H medium, NC=S medium. It was considered that the vibration energy propagated fast when a medium have a big elasticity and density.

• Journal of Plant Biology
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• 제36권3호
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• pp.211-218
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• 1993

Plant regeneration was accomplished from protoplast culture of rice (Oryza sativa L. cv. Taebaeg). Embryogenic callus was induced from mature seed on MS medium containing 5 mM proline, 2.5 mg/L 2,4-D, 30 g/L sucrose in the dark at 28$^{\circ}C$ and used to establish embryogenic cell suspension culture. Suspension cells were subcultured every one week in N6 medium supplemented with 5 mM proline, 200 mg/L casein hydrolysate, 2.5 mg/L 2,4-D and amino acids of AA medium. Suspension cultures were composed of cells that were densely cytoplasmic, potentially embryogenic and were at least maintained for more than 6 months in liquid medium. Protoplasts were isolated from fast-growing suspension culture cells and cultured in a slightly modified KpR medium by mixed nurse culture. Isolated protoplasts began to divide within 5~7 days and thereafter, protoplast-derived calli were sequentially transferred to callus proliferating medium that soft agar MS medium contained 2 mg/L 2,4-D and produced distinct embryogenic cells. Microcolonies were then transferred to solid medium which consisted of MS medium containing 5 mg/L kinetin, 1 mg/L NAA, 1 mg/L ABA, 30 g/L sucrose and 10 g/L sorbitol under fluorescent light. Mulitple shoots of 4~5 per callus emerged and were transferred to hormone-free MS medium for root initiation. Thereafter, The plantlets were transferred to pots of soil to mature in the culture room.

• Journal of Information Technology Applications and Management
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• 제10권3호
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• pp.109-120
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• 2003

This paper proposes an e-business maturity model for small and medium-sized enterprises. The model consists of five maturity levels, which are on-line access, on-line business, matured on-line business, and full on-line business from least matured level to most matured level. The model can be used to assess e-business maturity level of individual enterprises. individual enterprise's maturity level can be assessed by examining sample check lists. Additionally, this paper summarizes the results of an application of the proposed model to a couple of small and medium-sized enterprises. This model can also be used to suggest what individual enterprises need to be upgraded towards higher maturity level.

• Advances in nano research
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• 제4권4호
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• pp.309-326
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• 2016

The buckling response of a single-layered graphene sheet (SLGS) embedded in visco-Pasternak's medium is presented. The nonlocal first-order shear deformation elasticity theory is used for this purpose. The visco-Pasternak's medium is considered by adding the damping effect to the usual foundation model which characterized by the linear Winkler's modulus and Pasternak's (shear) foundation modulus. The SLGS be subjected to distributive compressive in-plane edge forces per unit length. The governing equilibrium equations are obtained and solved for getting the critical buckling loads of simply-supported SLGSs. The effects of many parameters like nonlocal parameter, aspect ratio, Winkler-Pasternak's foundation, damping coefficient, and mode numbers on the buckling analysis of the SLGSs are investigated in detail. The present results are compared with the corresponding available in the literature. Additional results are tabulated and plotted for sensing the effect of all used parameters and to investigate the visco-Pasternak's parameters for future comparisons.

• 한국의류학회지
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• 제2권1호
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• pp.157-166
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• 1978

The purpose of this study was; (1) to examine ready-to-wear dresses to find the characteristics of labels and construction which existed at three different price levels and (2) to make the gathered information available to consumers, designers, manufacturers and the people who are concerned with ready-to-wear garments. A check sheet was made listing a wide variety of features which could cause garments to be different. For the statistical analysis. 87 dresses were used out of 116 dresses examined. Observation was made at the stores in Myung Dong, Suh-Dae Moon and No Ryang Jin in Seoul from February 16, 1978 to March 3, 1978. The findings are; 1. The average prices of high, medium and low priced levels were ${\\}54,728$, ${\\}41,448$ and ${\\}7,225$. The average price discrepancy between high and medium priced levels was ${\\}13,000$ and ${\\}34,194$ between medium and low priced levels. 2. Most of the dresses examined had labels in all three priced levels. The most informative labels were found on medium priced dresses and then high. The low priced dresses had no information which would be of help to the consumer in regard to care, size and Jiber content. 3. Most of the dresses examined were cut on lengthwise grain. The stitching of medium and high priced levels were satisfactory. The chief way of fastening threads at the end of stitching was by backtracking. $28\%$ of the low priced levels was found unappropriate in stitching number because it was too large to the fabric. Many dresses in low priced levels were not pressed well. The average hem width of low priced dresses were less than 3cm, the high 3-4cm and the medium 4-5cm. Hemming was done mostly by hand in high and medium and by machine in low priced dresses. The medium priced dresses used more linings than low and high priced dresses.

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.203-207
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• 1998

Fed-batch fermentations with different feeding media were carried out in order to increase the productivity of invertase expression using a recombinant Saccharomyces cerevisiae containing plasmid pRB58. Two batch cultures showed the expression of the SUC2 gene at a low concentration of glucose, suggesting that glucose concentration could be used as a control variable in a fed-batch operation mode. In the fed-batch culture by feeding the basal medium, cell mass and specific invertase activity did not increase much as compared with the simple batch culture. A series of fed-batch cultures revealed that the sucrose-supplemented medium increased cell mass whereas the enriched medium did specific invertase activity. To capitalize on the synergism of the sucrose-supplemented medium and the enriched medium, the sucrose-supplemented enriched medium was used as a feeding medium. The fed-batch culture using this medium resulted in a 2.4-fold increase in cell mass and a 1.9-fold enhancement in specific invertase activity compared with those of the batch culture. The increase in cell mass and specific invertase activity led to a marked increase in total invertase activity, 250U/ml, which was 6.3 times higher than that of the batch culture.

• 미생물과산업
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• 제26권1호
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• pp.18-30
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• 2000

질소 고정능이 있는 Nostoc pruniforme를 재료로, 3종의 유기물, glucose, oxalic, 및 ethanol을 N-free medium과 NO$^{[-993]}$ riched medium에 처리해 주었을 때 다음과 같은 효과를 얻었다. 1)N-free medium과 NO$^{[-993]}$ riched medium에서 위의 유기물 모두가 질소 고정과정의 energy원으로서 또는 substrate로서의 유효한 결과를 얻었다. 3)oxalic acid를 처리한 실험에서는NO$^{[-993]}$ richedmedium의 경우 고농도 처리구에서 질소고정량이 많았고 N-free medium은 그 반대의 결과였다. 4)비교구인 control의 질소 고정량은 일예를 제회하고는 전반적으로 고농도 처리구와 저농도 처리구의 중간에 위치했다.

• The Plant Pathology Journal
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• 제30권4호
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• pp.432-436
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• 2014

The soil-borne ascomycete fungus Cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin A and radicicol. The slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. In this study, we developed a selective medium for C. destructans using radicicol produced by this fungus. Supplementing 50 mg/L of radicicol to medium inhibited the mycelia growth of other fungi including Botrytis cinerea, Rhizoctonia solani and Alternaria panax, but did not affect the growth of C. destructans. In addition, conidia germination of other fungal species except for C. destructans was inhibited in submerged culture supplemented with radicicol. This medium provides a very efficient tool for isolating C. destructans and also can be used as an enrichment medium for this fungus.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2010년도 춘계학술대회 논문집
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• pp.338-343
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• 2010

This paper's purpose is to support the strategies of the Small and Medium Railroad industries requiring certifications and products inspections. This study's objects are Small and Medium Railroad enterprises pursuing the making products, development and serving services. Research's questions consist of 14 principal questions and some incidental questions which are made for finding out what Small and Medium Railroad enterprises need. This research's result will be used for KRRI Railroad Testing & Certification Center's study. This study will include market research, deciding strategies and setting a range of supporting level for Small and Medium Railroad enterprises who request a test and certification service. After due consideration based on surveys, this paper makes clear what enterprises need actually, also indicates the strategic ways to support by technology transfer and test assistance for Small and Medium Railroad enterprises.