• Asian-Australasian Journal of Animal Sciences
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• 제14권9호
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• pp.1216-1220
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• 2001

Effect of hydroquinone (HQ) on rumen urease activity was studied. Hydroquinone at concentrations of 0.01 ppm, 0.1 ppm, 1 ppm, and 10 ppm inhibited urease activity of intact rumen microbes in vitro by 25%, 34%, 55% and 64% respectively. In the presence of low concentrations of $\beta$-mercaptoethanol, rumen urease could be solubilized and partially purified. The Km for the enzyme was $2{\times}10^{-3}$ M with Vmax of $319.4{\mu}moles/mg$ min. The kinetics of inhibition with partially purified rumen urease was investigated. The result showed that the inhibitory effect was not eliminated by increasing urea concentrations indicating a noncompetitive effect in nature with an inhibition constant $1.2{\times}10^{-5}$ M. Hydroquinone at the concentration of 10 ppm produced 64% urease inhibition, did not affect ruminal total dehydrogenase and proteolytic enzyme (p>0.05), but increased cellulase activity by 28% (p<0.05) in vitro. These results indicated that hydroquinone was a effective inhibitor of rumen urease and could effectively delay urea hydrolysis without a negative effect. The inhibitor appeared to offer a potential to improve nitrogen utilization by ruminants fed diets containing urea.

• Archives of Pharmacal Research
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• 제20권3호
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• pp.275-279
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• 1997

Helicobacter pylori (H. pylorl) infection is now established as the major pathogenic factor in chronic gastritis and peptic ulcer disease. in addition, there is accumulating evidence that H. pylori plays an important role in the process of gastric carcinogenesis. On the other hand, oriental traditional medicines have been used for stomach disease for thousands of years. In the present study, methanol extract from the stem bark of Magnolia sieboldii (M. sieboldii) and its components were investigated on their inhibitory effects against urease activity and growth of H. pylori in vitro. The methanol extract of M. sieboldii significantly inhibited the growth of H. pylori ATCC 43504 at 5 mg/ml. From the further fractionation, the chloroform fraction inhibited the bacterial growth dose-dependently. Among four fractions separated from the chloroform fraction by silica gel column chromatography, MS-C-2 was the most potent. Costunolide was isolated from the MS-C-2 subtraction by preparative TLC and recrystallization using n-hexane. Anti-H. pylori effect of costunolide was investigated using one commercial strain (H. pylori ATCC 43504) and three clinical strains (H. pylon 4, 43, 82548). Costunolide exhibited potent anti-H. pylori activity, and the MIC was around $100-200{\mu}g/ml$. However, costunolide had no inhibitory effect of H. pylori urease activity at the concentration used for the growth inhibition assay. From these results, we conclude that costunolide inhibits the, growth of H. pylori by the independent manner of H. pylori urease inhibition.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.140-145
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• 2006

The behaviour of alginate immobilized and soluble watermelon (Citrullus vulgaris) urease in water miscible organic solvents like, acetonitrile, dimethylformamide (DMF), ethanol, methanol, and propanol is described. The organic solvents exhibited a concentration dependent inhibitory effect on both the immobilized and the soluble urease in the presence of urea. Pretreatment of soluble enzyme preparations with organic solvents in the absence of substrate for 10 min at $30^{\circ}C$ led to rapid loss in the activity, while similar pretreatment of immobilized urease with 50% (v/v) of ethanol, propanol, and acetonitrile was ineffective. Time-dependent inactivation of immobilized urease, both in the presence and in the absence of urea, revealed stability for longer duration of time even at very high concentration of organic solvents. The soluble enzyme, on the other hand, was rapidly inactivated even at fairly lower concentrations. The results suggest that the immobilization of watermelon urease in calcium alginate make it suitable for its application in organic media. The observations are discussed.

• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.1057-1065
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• 2002

The antagonistic activities of 30 strains of lactobacilli against Helicobacter pylori were determined and Lactobacillus helveticus CU631 has been selected as the strain which possesses the strongest inhibitory effect in the disc diffusion assay showing inhibition zone diameter of $10{\pm}1.5mm$, whereas those of L. plantarum and L. fermentum have been shown to be $4.0{\pm}0.6mm$. H. pylori G88016 revealed the highest vacuolating toxin producing activity among the 8 strains, the inhibitory activity of L. helveticus CU631 in vacuolating toxin producing activity of H. pylori manifested in the co-culture of two strains and in the 5:5 mixture of supernatant of the two strains. Both L. helveticus CU631 and cell free culture supernatant had a strong inhibitory activities in urease and cytotoxin producing activities of H. pylori NCTC11637 and CJH12. An accelerated proteolytic activity of water soluble peptides by L. helveticus CU631 during the refrigeration storage has been manifested in the cream cheese. DNA seqences of 16S-23S ribosomal RNA spacer region showed typical pattern among the various strains of L. helveticus, which could be used in the identification of L. helveticus CU 631.

• Archives of Pharmacal Research
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• 제19권6호
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• pp.447-449
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• 1996

Inhibitory effects of the mushrooms on the growth and urease of Helicobacter pylori (HP), which is associated with human gastroduodenal diseases such as gastritis, peptic ulcer and gastric carcinoma, were investigated. Most of the mushroom extracts did not show inhibitory effect on HP urease except Coriolus versicolar, Auricularia auricular Sarcodon aspratus and Flammulina velutipes. The extract of Ganoderma lucidum, Coriolus versicolar, Gyropora esculenta and Agaricus bisporus var. albidus inhibited the growth of HP. When their extracts were fractionated, the ether fraction of Ganoderma lucidum and Agaricus bisporus var. albidus were the most effective. Among seven components separated from the ether fraction of G. lucidum extract by silica gel column chromatography, P3 was the most potent: MIC was $200{\mu}g/ml$. However, P3 did not inhibit the urease.

• 미생물학회지
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• 제50권1호
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• pp.60-66
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• 2014

본 연구에서는 Lactobacillus brevis HLJ59 균주를 이용하여 오미자 당침액의 발효특성을 조사하였다. 발효에 따른 생균수와 PPH radical 소거활성, 환원력 및 SOD 유사활성을 조사하였다. 또한 오미자 당침액의 ACE 저해활성 및 Urease 저해활성을 조사하였다. 생균수의 경우 2배, 5배, 10배 희석액의 경우 $2.05{\pm}0.21{\times}10^8$, $6.31{\pm}0.56{\times}10^{11}$, $8.14{\pm}0.14{\times}10^9$으로 각각 조사되었으며, DPPH radical 소거활성은 2배, 5배, 10배 희석액의 경우 60.3%, 71.8%, 44.5%로 각각 조사되었다. 환원력은 2배, 5배, 10배 희석액의 경우 0.92, 1.19, 0.73 (700 nm에서의 OD값)로 각각 조사되었고, SOD 유사활성은 2배, 5배, 10배 희석액의 경우 50.4%, 53.7%, 33.4%로 각각 조사되었다. 그리고 ACE 저해활성 및 Urease 저해활성은 2배, 5배, 10배 희석액의 경우 47.4%, 78.2%, 56.4% 및 58.1%, 83.4%, 63.2%로 각각 조사되었다. 이상의 결과에서 오미자 당침 희석액 발효물은 우수한 항산화활성과 ACE 및 Urease 저해활성을 지님으로서 이를 기능성 식품소재로 활용할 수 있다고 사료된다.

• 한국식품과학회지
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• 제35권6호
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• pp.1182-1187
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• 2003

위질환 원인균으로 알려진 H. pylori 생육을 저해하는 물질을 탐색하기 위하여 호장근 메탄을 추출물 및 분획물을 이용하여 H. pylori에 대한 항균실험 및 urease 활성 억제효과를 검토하였다. 호장근 메탄올 추출물의 항균실험을 실시한 결과 5mg/cylinder의 농도일 때 inhibition zone이 17mm 정도로 높은 항균성을 가지는 것으로 나타났으며, 분획물의 경우 1mg/cylinder의 농도일 때 hexane 분획과 chloroform 분획물에서 각각 24mm, 17mm로 높은 항균 활성을 보였다. H. pylori의 crude urease를 분리하여 urea broth에서 흡광도와 pH를 조사한 결과, 반응 2시간 후 pH가 8.35까지 증가하였고 $O.D._{560nm}$에서의 흡광도는 0.859까지 증가하였다. 호장근에 의한 urease 활성억제는 호장근의 메탄올 추출물의 농도가 0.5 mg/mL에서 대조구에 비해 35% 억제효과를 보였고, 2 mg/mL에서는 85% 이상으로 매우 높은 억제활성을 보였다. 또한. 각 분획물의 저해 효과를 10배 농도의 메탄올추출물과 비교하였을 때. $20\;{\mu}g/mL$의 농도에서 water 분획을 제외한 모든 분획물들이 반응 2시간 후 $30{\sim}40%$의 저해를 보여 $200\;{\mu}g/mL$ 농도의 메탄올추출물이 20% 이하의 저해를 보인 것과 비교해 높은 활성을 보였다. 호장근 메탄올 추출물 처리에 의한 H. pylori의 형태변화를 조사하기 위하여 주사 및 투과전자 현미경으로 균의 형태를 관찰하였다. 주사전자 현미경 관찰 시 대조구는 균체 표면이 깨끗하고 완전한 구형을 이루고 있는 반면, 호장근 처리구는 균체 표층 구조가 심하게 허물고 떨어져 나간 것을 볼 수 있었고 또한 세포가 팽대하였고 일부 세포벽은 완전히 파괴되어 균체내 성분이 모두 유출되어 균 형태가 없어져 버린 것도 관찰되었다.

• Journal of Applied Biological Chemistry
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• 제58권1호
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• pp.89-95
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• 2015

Helicobacter pylori infection causes gastrointestinal diseases such as chronic gastritis, peptic ulcers, and may lead to gastric cancer. Several studies have reported that lactobacilli present on broccoli show inhibitory activity against H. pylori. Here, we evaluated aqueous broccoli, fermented by Lactobacillus plantarum MG208, for its fermentation characteristics and anti-H. pylori activities including antibacterial activity, growth inhibition, anti-adhesion, and urease inhibition. The results indicated that the fermentation characteristics changed significantly depending on the amount of aqueous broccoli used for fermentation (p <0.05). There was no significant difference between the samples before fermentation (p >0.05). However, a significant concentration-dependent difference was noted in antibacterial activity and urease inhibition (p <0.05) following the addition of aqueous broccoli. Growth inhibition in the 10 mg/mL sample was significantly higher as compared to the negative control and similar to that with amoxicillin (positive control) (p <0.05). Anti-adhesion activity of aqueous broccoli was also significantly different (p <0.05) from the negative control. Therefore, aqueous broccoli fermented by L. plantarum MG208 could prove useful as a functional diet for protection of the gastric environment against H. pylori infection.

• 생약학회지
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• 제49권3호
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• pp.255-261
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• 2018

The aim of this study was to evaluate the anti-helicobacter activity and anti-inflammatory activity of Angelica dahurica (AD). The minimum inhibitory concentration(MIC) of AD against Helicobacter pylori(H. pylori), expression of the H. pylori cagA gene in the presence of AD was determined. Inhibition of H. pylori urease by AD, inhibition of nitric oxide (NO) production in AGS cells was measured. IL-8 mRNA expression in AGS cells which were infected with H. pylori and IL-8 level was measured. The MIC of MeOH Ex. of AD was $250{\mu}g/mL$ and the expression of cagA gene was decreased about 88% in the presence of AD. The activity of H. pylori urease was inhibited 70% by AD. mRNA expression of IL-8 and the production of NO and IL-8 were significantly decreased in the presence of AD. In conclusion, AD showed anti-Helicobacter activity and has potent anti-inflammatory effect on H. pylori-induced inflammation in human gastric epithelial AGS cells.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.532-534
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• 2000

The inhibitory effects of different Bifidobacterium spp. on the growth of Helicobacter pylori (HP) were investigated. A significant suppression of HP growth occurred only when HP was inoculated onto a petri dish containing 0.1 mg/ml of Bifidobacterium spp. When HP was separately cultured with B. breve K-110, B. catenulatum K-309, B magnum K-311, B. magnum K-321, and B. cuniculi K-513, the urease activity was also inhibited by these Bifidobacterium spp. Therefore, it appears that these Bifidobacterium spp. excrete a heat-labile inhibitory component for HP growth into the culture medium. Although most organic acids produced by the Bifidobacterium spp. inhibited the growth of HP, the HP growth was not inhibited by the physiological concentrations of organic acids produced in bifidobacteria-cultured media. Accordingly, these results suggest that some Bifidobacterium spp. may produce antibiotic-like compounds (bacteriocins).