• The Journal of Internal Korean Medicine
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• v.31 no.3
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• pp.631-649
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• 2010

Objective : The object of this study was to observe the effects of aqueous extracts of Chunglijagam-tang(CLJGT), which has traditionally been used in Korean medicine for treating various diseases, on streptozotocin(STZ)-induced rat diabetes and related complications: diabetic nephropathy, hepatopathy and hyperlipemia. Methods : CLJGT extracts were orally administered once a day for 28 days at a dosage 50, 100 and 200mg/kg from 21 days after STZ treatment, and the changes on body weights, blood glucose levels, kidney and liver weights, serum BUN(blood urea nitrogen), creatinine, AST(aspartate transaminase), ALT(alanine aminotransferase), HDL(high-density lipoprotein), LDL (low-density lipoprotein), total cholesterol and triglyceride levels were observed with pancreatic malondialdehyde(MDA) and glutathione(GSH) contents. The results were compared with silymarin 100mg/kg. Results : Significant decrease of blood glucose levels, kidney and liver weights, serum BUN, creatinine, AST, ALT, LDL, total cholesterol, triglyceride levels, pancreatic malondialdehyde contents and significant increase of body weights, serum HDL levels, pancreatic glutathione contents were detected in CLJGT extracts 50, 100 and 200mg/kg administered groups as compared to the STZ control group. Conclusion : CLJGT extracts showed favorable effects on the STZ-induced diabetes and related complications mediated by their antioxidant effects as similar to silymarin. Therefore, it is expected that DBEH has potential for use in the management of diabetes and various diabetic complications.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.21-29
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• 2001

An experiment was conducted with twenty crossbred male lambs to assess the effect of cotton (Gossypium) seed meal (CSM) on blood constituents and immunity. Lambs were randomly assigned to a reference diet (30% deoiled peanut meal, DPNM) and four test diets containing 40% of either raw, 45 minutes cooked, 1% $Ca(OH)_2$ and iron (1 free gossy-pol, FG : 0.3 Fe) treated CSM (replacing approximately 50%, reference concentrate mixture). These isonitrogenous and isocaloric concentrate mixtures were fed to meet 80% of protein requirements (NRC, 1985) along with ad lib maize hay for 180 days. Blood was collected at 60, 120 and 180 days post feeding. The lambs were sensitized with Brucella abortus S99 antigen after 140 days and were subjected to ELISA and delayed type hypersensitivity. Blood haemoglobin, erythrocyte count, leucocyte count, total protein, total albumin, total globulin, urea, creatinine concentration and aspartate aminotransferase activity in lambs fed on raw or processed CSM were comparable to the values of reference lambs. The higher (p<0.01) blood glucose levels observed in CSM fed lambs at 60 days of feeding was latter reduced to the levels comparable with those on reference diet at 120 and 180 days of feeding. The alanine amino transferase activity was lower in lambs fed raw and cooked CSM containing diets at 120 and 180 days of feeding. A marginal increase in serum iron and alkaline posphatase activity was observed in iron treated group and raw CSM fed lambs, respectively. The humoral immune response and DTH reactivity was lower (p<0.05) in lambs fed raw CSM (consuming 302.83 mg FG/day). Cooking, $Ca(OH)_2$ and iron treatment of raw CSM showed a positive response in alleviating the suppression of immune response owing to the reduced consumption of FG by 40.19, 17.40% and 26.73%, respectively in these diets. The present study thus indicated that consumption of 40% raw CSM (302.83 mg FG/day) though did not affect majority of the haematological and blood biochemical parameters, but markedly suppressed the immune mechanism of lambs.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.10
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• pp.1449-1457
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• 2008

This experiment was carried out to evaluate the carcass characteristics, chemical composition and fatty acid profile of the Longissimus muscle (LM) of three cattle genetic groups (Purunã, PUR, 11; 1/2 Purunã vs. 1/2 British, PUB, 6 and 1/2 Charolais vs. 1/2 Caracu, CHC, 10) finished in pasture systems. The field work took place at the Lapa Research Farm of the Agronomic Institute of Paraná, in the city of Lapa, south Brazil. The animals were fed during the winter with corn silage, cottonseed meal, cracked corn, urea, limestone and mineral salts as sources of protein, as well as an energy supplement, in pasture systems of Brachiaria decumbens Stapf. The animal groups were slaughtered at 20 months of age, at 50122.6 kg live weight. CHC bulls had higher (p<0.05) final weight than PUR and PUB bulls. Hot carcass weight was similar (p>0.10) between PUR and PUB. Hot carcass dressing percentage was higher (p<0.05) for PUB bulls than for PUR and CHC bulls. On the other hand, hot carcass dressing percentage was similar (p>0.05) between PUR and CHC bulls. Fat thickness was similar (p>0.10) among all genetic groups. However, the Longissimus area of CHC bulls was greater (p<0.05) than in PUR and PUB genetic groups. The genetic groups did not affect (p>0.10) the marbling of Longissimus. There was no observed difference (p>0.10) in moisture, ash, crude protein and total cholesterol contents among the three genetic groups. On the other hand, the total lipid percentage was higher (p<0.05) for the PUB genetic group in comparison with PUR and CHC. CLA percentage was highest for PUR animals. However, total CLA amounts were not altered by the different genetic groups.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6721-6726
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• 2014

Background: The aim of this preliminary study was to address variations of responses observed with different starting tumor sizes of 10 and 15 mm, and the effects of different doses of tamoxifen (TAM) on experimental rat mammary tumors. Materials and Methods: Thirty-five inbred female Sprague Dawley rats aged 43 days were administered with three weekly doses of N-methyl-N-nitrosourea (NMU) intraperitoneally (ip) at 50 mg/kg body weight. Animals were randomized (beginning from 10 mm tumor size) into four TAM-treated (50, 100, 200 and $500{\mu}g/day$) groups of six animals each, and another group (n=6) treated with TAM $100{\mu}g/day$ at starting tumour size of 15 mm. The animals were treated by oral gavage daily for 8 weeks before sacrifice. Results: Serum urea and creatinine, and overall physical tumor burden were significantly modulated in animals treated with variable doses of TAM compared to the untreated controls (n=5). Final body weight and tumor number were significantly different in the 10 mm-treated animals compared to those treated at 15 mm. There were no significant differences in histopathological features among all the groups. Conclusions: Our findings suggest the importance of standardizing tumour size and drug doses before initiation of treatment, particularly in the direct comparison of basic end-tumour physical parameters.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.157-167
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• 2000

A thermostable chitosanase gene from the isolated strain, Bacillus sp. CK4, was cloned, and its complete DNA sequence was determined. The thermostable chitosanase gene was composed of an 822-bp open reading frame which encodes a protein of 242 amino acids and a signal peptide corresponding to a 30 kDa enzyme in size. The deduced amino acid sequence of the chitosanase from Bacillus sp. CK4 exhibits 76.6%, 15.3%, and 14.2% similarities to those from Bacillus subtilis, Bacillus ehemensis, and Bacillus circulans, respectively. C-terminal homology analysis shows that Bacillus sp. CK4 belongs to the Cluster III group with Bacillus subtilis. The size of the gene was similar to that of a mesophile, Bacillus subtilis showing a higher preference for codons ending in G or C. The functional importance of a conserved region in a novel chitosanase from Bacillus sp. CK4 was investigated. Each of the three carboxylic amino acid residues were changed to E50D/Q, E62D/Q, and D66N/E by site-directed mutagenesis. The D66N/E mutants enzymes had remarkably decreased kinetic parameters such as $V_{max}$ and k$\sub$cat/, indicating that the Asp-66 residue was essential for catalysis. The thermostable chitosanase contains three cysteine residues at position 49, 72, and 211. Titration of the Cys residues with DTNB showed that none of them were involved in disulfide bond. The C49S and C72S mutant enzymes were as stable to thermal inactivation and denaturating agents as the wild-type enzyme. However the half-life of the C211S mutant enzyme was less than 60 min at 80$^{\circ}C$, while that of the wild type enzyme was about 90 min. Moreover, the residual activity of C211S was substantially decreased by 8 M urea, and fully lost catalytic activity by 40% ethanol. These results show that the substitution of Cys with Ser at position 211 seems to affect the conformational stability of the chitosanase.

• Korean Journal of Veterinary Research
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• v.48 no.3
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• pp.347-355
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• 2008

Perfluorooctanoic acid (PFOA), a member of the perfluoroalkyl acids that have wide commercial applications, is persistent organic pollutants widely spread throughout the environment and human population. But little is known about the adverse biological effects of the PFOA. In the present study, the toxicological effects of PFOA were investigated in rats. Sprague-Dawley rats (N = 10 in each group) were orally administered with PFOA in drinking water for 4 weeks (0, 100, 200, or 400 ppm in male, and 0, 200, 400, or 800 ppm in female). Three female rats given 800 ppm died during the study. PFOA treatment decreased the body weight gain and increased the liver weights in both genders. Serum biochemical investigations revealed significant increases in the aspartate aminotransferase, alanine transaminase, alkaline phosphatase, blood urea nitrogen, total cholesterol, and total bilirubin in male but in female. Serum estradiol (E2) levels were increased in all treated rats. Histopathologically, hepatocellular hypertrophy around central vein was noted in the liver of treated rats. No significant histopathological changes were noted in other organs. In conclusion, PFOA induced toxicological changes in the liver and increased serum E2 level which was not related to histopathological changes of endocrine and reproductive system.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.6
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• pp.1253-1259
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• 2002

This study was carried out to investigate the effects of Bambusae Caulis in Liquamen from different production process on blood sugar of the diabetic mice induced with Streptozotocin(STZ). The original Bambusae Caulis in Liquamen filtered and refined. Bambusae Caulis in Liquamen D(H-BCL.D) extracted at high temperature(1000℃), Bambusae Caulis in Liquamen A(L-BCL.A) and Bambusae Caulis in Liquamen B(L-BCL.B) extracted at low temperature (250～450℃) were administerd to mice for 4weeks and its anti-diabetic effect examined. Mice used in this experiment were divided into four groups(Control, H-BCL.D, L-BCL.A and L-BCL.B). Experemental groups were observed in terms of blood sugar, Creatinine, BUN and GPT. The amount of glucose was significantly decreased in the Bambusae Caulis in Liquamen-treated groups compared with the control(P < 0.05). The amount of Creatinine did not show any differences among four groups. The amount of Blood Urea Nitrogen did not show any differences in the L-BCL.A and B-treated groups, but did show slightly decrease(P<0.05) at H-BCL.D-treated group. The amount of GPT did not show any differences among four groups. In conclusion, it was found that Bambusae Caulis in Liquamen from extracted at high or low temperature were effective on murine hyperglycemia mice induced with STZ respectively.

• Toxicological Research
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• v.30 no.2
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• pp.99-107
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• 2014

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.4
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• pp.337-342
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• 1995

Two feeding trials were conducted to determine the effect of molasses feeding on the growth performances of bulls. In the first experiment, the straw of a control diet of straw and urea was replaced by molasses of 150 g ($M_{150}$) or $300g{\cdot}kg^{-1}$ ($M_{300}$). The three diets were fed ad libitum to three groups of bulls having five in each and the dietary responses were statistically analyzed in a simple design. The bulls were given fishmeal ($30g{\cdot}hd^{-1}{\cdot}d^{-1}$), wheat bran (0.5% of Liveweight, LW) and allowed grazing for $5hrs{\cdot}d^{-1}$. Molasses supplementation significantly (p < 0.001) increased the dry matter intake (DMI) of the bulls of $M_{150}$ (35.8g) and of $M_{300}$ (42.9g) than the control ($30.4g{\cdot}kg^{-0.75}$). The daily LW gain of the bulls of the $M_{150}$ (982g) was significantly (p < 0.05) higher than the bulls of the control or of the $M_{300}$ diet. In the second experiment, a control group of four bulls was fed a straw diet. Treatment two groups, having four bulls in each, were fed a mixed diet of Leucaena leucocephala and Setaria splendida(1:1, DM basis) with (LSM) and without (LS) molasses at $100g{\cdot}kg^{-1}$. The dietary responses were statistically analyzed in a simple design. The bulls of the LSM diet had significantly (p < 0.05) higher DMI, DM digestibility and LW gain ($128g{\cdot}kg^{-0.75}$, $663g{\cdot}kg^{-1}$ and $419g{\cdot}d^{-1}$, respectively) than the bulls of the LS diet ($98.3g{\cdot}kg^{-0.75}$, $583g{\cdot}kg^{-1}$ and $292g{\cdot}d^{-1}$, respectively). But the differences between the LSM and the control were nonsignificant. It may be concluded that molasses may be utilized as supplement to straw at $150g{\cdot}kg^{-1}$ or forages at $100g{\cdot}kg^{-1}$ diet for the profitable beef production from young growing bulls.

• The Journal of Natural Sciences
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• v.9 no.1
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• pp.39-44
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• 1997

This study was aimed to observe the effects of municipal sewage sludge as nutrients on the growth of Lettuce. The effects of heavy metal components of the sludge were also observed. Municipal sewage sludge from Taejon City were treated to the soil of PaiChai University Farm in order to make gradient of nitrogen concentration in soil among 6 experimental groups. Results are as follows ;The longest leaves and roots were produced in control pot, in which urea was supplied as nitrogen source to the soil. Any other sludge-treated groups produced shorter leaves and roots than control group, but the more sewage sludge treated the longer leaves and heavier vegetables were observed. No differences were observed among from 6 experimental groups in the view of N, K, Ca and Mg contents. But P contents in leaves from 6 groups varied. Zn content of Lettuce when was 65.8 mg/kg when it grew with sludge 200%. The groups treated with sludge 200% produced the vegetables of highest Zn contents. Accumulation of Zn, Mn and Cd was more heavier in Lettuce than in Altari radish. Accumulation of Zn, Mn, Cu and Cd in lettuce was not heavier than natural contents of those in market vegetables.