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Stereoselective Microbial Hydroxylation of Progestin, Norethisterone by Using Aspergillus niger and Penicillium citrinum

  • Azizuddin, Azizuddin;Iqbal, Muhammad;Musharraf, Syed Ghulam;Shahzad, Saleem
    • Natural Product Sciences
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    • v.26 no.4
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    • pp.283-288
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    • 2020
  • Microbial transformation of a potent progestin, norethisterone (17��-hydroxy-19-nor-17α-pregn-4-en-20-yn-3-one) (1) was carried out by using two filamentous fungi Aspergillus niger and Penicillium citrinum. Biotransformation of 1 with A. niger yielded a hydroxylated transformed product 10��,17��-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (2) whereas 11��,17��-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (3) was obtained through microbial transformation of 1 by P. citrinum. It is the first report of their production from 1 by using A. niger and P. citrinum with complete 1H- and 13C-NMR assignment. The structures of both metabolites were characterized by various spectroscopic techniques and reported data.

Exploring the Catalytic Significant Residues of Serine Protease Using Substrate-Enriched Residues and a Peptidase Inhibitor

  • Khan, Zahoor;Shafique, Maryam;Zeb, Amir;Jabeen, Nusrat;Naz, Sehar Afshan;Zubair, Arif
    • Microbiology and Biotechnology Letters
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    • v.49 no.1
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    • pp.65-74
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    • 2021
  • Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.

The effects of traditional frying method on proximate composition and energetic values of fish species from Karachi coast of Pakistan

  • Zafar, Fatima Hayat Shaheen;Zahid, Mohammad;Bat, Levent
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.2
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    • pp.35-43
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    • 2019
  • The effect of pan frying method was evaluated for proximate composition of selected fish species Pampus argenteus, Lethrinus nebulosus and Acanthopagarus arabicus which were determined by standard procedures. Proximate compositions found in the frying fillets were different to the raw fish samples. The moisture content seems to decrease in all fried samples. The reason of moisture loss at the time of frying produced is excessive protein amount in fried fish as compare to raw fish. Protein content seemed to increase in Pampus argentus > Lethrinus nebulosus and >Acanthopagrus arabicus in their particular fried form. The outcomes shown that protein content was in the ranges of 14.83-25.07 g/100g in fried samples, while the fat content was between 3.76-9.77 g/100 g. The energy content was the highest in the fried fish Lethrinus nebulosus (236 kcal/100 g), Pampus argentus (161 kcal/100 g) and Acanthopagrus arabicus (135 kcal/100 g). The information achieved in this study would be very important to fish consumers, processors and nutritionists to academically increase their awareness regarding the nutrients contents in selected fish species.

ON PSEUDO-SLANT SUBMANIFOLDS OF A NEARLY (ε, δ)-TRANS SASAKIAN MANIFOLD

  • Jun, Jae-Bok;Rahman, Shamsur
    • Communications of the Korean Mathematical Society
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    • v.34 no.3
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    • pp.935-949
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    • 2019
  • The purpose of the paper is to study the notion of pseudo-slant submanifolds and the existence of some structures on a pseudo-slant submanifolds of nearly (${\varepsilon},{\delta}$)-trans-Sasakian manifold. Totally umbilical proper-slant submanifolds are worked out. We discuss the integrability of distributions on pseudo-slant submanifolds of nearly (${\varepsilon},{\delta}$)-trans-Sasakian manifold.

BRIEF HISTORY OF TRANSLATION IN UNANI MEDICINE WITH MAJOR MILESTONES: A GLIMPSE

  • Ansari, Shabnam
    • CELLMED
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    • v.9 no.3
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    • pp.1.1-1.6
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    • 2019
  • Unani medicine has been used extensively as preventive and therapeutic healthcare in India. It mostly utilizes herbal drugs for the treatment of various conditions and ailments. It is based upon the humoural and temperament theory of Buqrat (Hippocrates). Unani medicine is one of the oldest traditional system rooted within the Greek, Iranian, Arabic, and Islamic medical knowledge and has developed as a scientific healthcare system. It is highly practiced and popular in certain parts of the world, and the World Heritage Centre, part of the United Nations Educational, Scientific, and Cultural Organization (UNESCO), and the United Nations Foundation list it as an authentic and still-living form of traditional medicine. But in the past, its survival required utmost efforts from different dynasties, scholars and organization around the world. The efforts of promotion, preservation, translation, upgradation and publication of medical knowledge has crucially given new life to Unani medicine in each era. This letter will enlighten the efforts of translations of medical knowledge in Unani medicine from its arising need in the past till present.

An Arabic Script Recognition System

  • Alginahi, Yasser M.;Mudassar, Mohammed;Nomani Kabir, Muhammad
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.9
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    • pp.3701-3720
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    • 2015
  • A system for the recognition of machine printed Arabic script is proposed. The Arabic script is shared by three languages i.e., Arabic, Urdu and Farsi. The three languages have a descent amount of vocabulary in common, thus compounding the problems for identification. Therefore, in an ideal scenario not only the script has to be differentiated from other scripts but also the language of the script has to be recognized. The recognition process involves the segregation of Arabic scripted documents from Latin, Han and other scripted documents using horizontal and vertical projection profiles, and the identification of the language. Identification mainly involves extracting connected components, which are subjected to Principle Component Analysis (PCA) transformation for extracting uncorrelated features. Later the traditional K-Nearest Neighbours (KNN) algorithm is used for recognition. Experiments were carried out by varying the number of principal components and connected components to be extracted per document to find a combination of both that would give the optimal accuracy. An accuracy of 100% is achieved for connected components >=18 and Principal components equals to 15. This proposed system would play a vital role in automatic archiving of multilingual documents and the selection of the appropriate Arabic script in multi lingual Optical Character Recognition (OCR) systems.

Logistics Capability, Logistics Outsourcing and Firm Performance in Manufacturing Companies in Pakistan

  • AZIZ, Abdul;MEMON, Javed Ahmed;ALI, Shuaib
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.8
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    • pp.435-444
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    • 2020
  • This study investigates the role of logistics capability and logistics outsourcing on the performance of manufacturing companies in Pakistan. It examines how logistics capability affects firm's performance, why outsourcing is essential and how firms benefited if they outsource the service rather than establishing their own logistics capability. This research is based on a survey using structured a questionnaire to collect the primary data. The target population is logistics specialists of manufacturing companies in Pakistan with head offices based on Karachi, that have their own logistics or outsource their logistics. The questionnaire has been distributed to 500 respondents in 113 manufacturing companies in Pakistan. Confirmatory factor analysis has been used as statistical techniques to check the factor loading of the components, and SEM (Structural Equation Model) is used to check the impact of logistics capability on firm's performance as well as the role of logistics outsourcing as a mediator. The findings of the research suggest logistics capability has positive impact on the performance of the manufacturing companies in Pakistan, and logistics capability has also an impact on logistics outsourcing. On the other hand, the study found that logistics outsourcing has no significant impact on the manufacturing companies in Pakistan.

Bloom to Gloom-Emotional Intelligence and Employee Silence: An Empirical Study from Pakistan

  • SAEED, Sadia;AKHTAR, Naveed;HUSSAIN, Shariq
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.12
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    • pp.497-507
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    • 2021
  • The objective of the current research study is to examine those elements/factors that can reduce silence so that organizations can become more efficient, innovative, and adaptable by sharing knowledge and work-related problems. The purpose behind conducting this research was to explore the effects of emotional intelligence on silent behavior. The study also focuses on other individual negative aspects that can increase counterproductive behavior like silence and examines the effects of emotional intelligence on silence through moral disengagement. Data was collected from 400 employees using stratified sampling to ensure adequate representation of males and females. Data was collected from nurses and young doctors using the adopted measurement scale through a self-administered questionnaire. Since the sample included nursing staff and they usually are not well versed in English, the instrument was translated into Urdu. Data were analyzed using SEM to assess the direct and indirect effects of EI on employee silence. The result indicates that emotional intelligence has a positive impact on employee silence and moral disengagement. In contradiction to theory, the findings suggest that people with high emotional intelligence tend to get morally disengaged. As a result, they will remain silent and withhold information regarding work-related issues and problems.

Application of Deep Learning: A Review for Firefighting

  • Shaikh, Muhammad Khalid
    • International Journal of Computer Science & Network Security
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    • v.22 no.5
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    • pp.73-78
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    • 2022
  • The aim of this paper is to investigate the prevalence of Deep Learning in the literature on Fire & Rescue Service. It is found that deep learning techniques are only beginning to benefit the firefighters. The popular areas where deep learning techniques are making an impact are situational awareness, decision making, mental stress, injuries, well-being of the firefighter such as his sudden fall, inability to move and breathlessness, path planning by the firefighters while getting to an fire scene, wayfinding, tracking firefighters, firefighter physical fitness, employment, prediction of firefighter intervention, firefighter operations such as object recognition in smoky areas, firefighter efficacy, smart firefighting using edge computing, firefighting in teams, and firefighter clothing and safety. The techniques that were found applied in firefighting were Deep learning, Traditional K-Means clustering with engineered time and frequency domain features, Convolutional autoencoders, Long Short-Term Memory (LSTM), Deep Neural Networks, Simulation, VR, ANN, Deep Q Learning, Deep learning based on conditional generative adversarial networks, Decision Trees, Kalman Filters, Computational models, Partial Least Squares, Logistic Regression, Random Forest, Edge computing, C5 Decision Tree, Restricted Boltzmann Machine, Reinforcement Learning, and Recurrent LSTM. The literature review is centered on Firefighters/firemen not involved in wildland fires. The focus was also not on the fire itself. It must also be noted that several deep learning techniques such as CNN were mostly used in fire behavior, fire imaging and identification as well. Those papers that deal with fire behavior were also not part of this literature review.